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The N\u2010half of the deduced amino acid sequence of 432 amino acids of CROP contains cysteine\/histidine motifs and leucine zipper\u2010like repeats. The C\u2010half consists mostly of charged and polar amino acids: arginine (58 residues or 25%), glutamate (36 residues or 16%), serine (35 residues or 15%), lysine (30 residues, 13%), and aspartate (20 residues or 9%). The C\u2010half is extremely hydrophilic and comprises domains rich in lysine and glutamate residues, rich in alternating arginine and glutamate residues, and rich in arginine and serine residues. The arginine\/serine\u2010rich domain is dominated by a series of 8 amino acid imperfect repetitive motif (consensus sequence, Ser\u2010Arg\u2010Ser\u2010Arg\u2010Asp\/Glu\u2010Arg\u2010Arg\u2010Arg), which has been found in RNA splicing factors. The RNase protection assay and Western blotting analysis indicate that the expression of CROP is about 2\u20133\u2010fold higher in mRNA and protein levels in cisplatin\u2010resistant ACHN\/CDDP cells than in host ACHN cells. CROP is the human homologue of yeast Luc7p, which is supposed to be involved in 5\u2032\u2010splice site recognition and is essential for vegetative growth.<\/jats:p>","DOI":"10.1016\/s0014-5793(99)01744-5","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T01:45:07Z","timestamp":1027647907000},"page":"153-156","source":"Crossref","is-referenced-by-count":24,"title":["CROP\/Luc7A, a novel serine\/arginine\u2010rich nuclear protein, isolated from cisplatin\u2010resistant cell line<sup>1<\/sup>"],"prefix":"10.1002","volume":"465","author":[{"given":"Yoichi","family":"Nishii","sequence":"first","affiliation":[]},{"given":"Masaki","family":"Morishima","sequence":"additional","affiliation":[]},{"given":"Yoshiyuki","family":"Kakehi","sequence":"additional","affiliation":[]},{"given":"Kouji","family":"Umehara","sequence":"additional","affiliation":[]},{"given":"Noriyuki","family":"Kioka","sequence":"additional","affiliation":[]},{"given":"Yoshitake","family":"Terano","sequence":"additional","affiliation":[]},{"given":"Teruo","family":"Amachi","sequence":"additional","affiliation":[]},{"given":"Kazumitsu","family":"Ueda","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2000,3,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-100-5-704"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(87)90198-5"},{"key":"e_1_2_6_4_1","first-page":"4313","volume":"54","author":"Thew K.D.","year":"1994","journal-title":"Cancer Res."},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00361a026"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-84150-7_2"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.6.2076"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00122a002"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00466a003"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.11.3123"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.7.3070"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(81)90411-7"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00413a022"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.25.14981"},{"key":"e_1_2_6_15_1","first-page":"5100","volume":"57","author":"Saleem A.","year":"1997","journal-title":"Cancer Res."},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118668"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(96)01150-7"},{"key":"e_1_2_6_18_1","unstructured":"Arao S. 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