{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T02:56:03Z","timestamp":1761101763727},"reference-count":34,"publisher":"Elsevier","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005]]},"DOI":"10.1016\/s0076-6879(05)98016-6","type":"book-chapter","created":{"date-parts":[[2005,11,8]],"date-time":"2005-11-08T12:12:00Z","timestamp":1131451920000},"page":"175-195","source":"Crossref","is-referenced-by-count":18,"title":["Large\u2010Scale Purification of the Vertebrate Anaphase\u2010Promoting Complex\/Cyclosome"],"prefix":"10.1016","author":[{"given":"Franz","family":"Herzog","sequence":"first","affiliation":[]},{"given":"Jan\u2010Michael","family":"Peters","sequence":"additional","affiliation":[]}],"member":"78","reference":[{"key":"10.1016\/S0076-6879(05)98016-6_bib0001","doi-asserted-by":"crossref","first-page":"8965","DOI":"10.1021\/bi973101r","article-title":"Ionization\u2010reactivity relationships for cysteine thiols in polypeptides","volume":"37","author":"Bulaj","year":"1998","journal-title":"Biochemistry"},{"key":"10.1016\/S0076-6879(05)98016-6_bib0002","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1038\/10049","article-title":"Temporal and spatial control of cyclin B1 destruction in metaphase","volume":"1","author":"Clute","year":"1999","journal-title":"Nature Cell Biol."},{"key":"10.1016\/S0076-6879(05)98016-6_bib0003","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1083\/jcb.129.3.725","article-title":"The Drosophila cell cycle gene fizzy is required for normal degradation of cyclins A and B during mitosis and has homology to the CDC20 gene of Saccharomyces cerevisiae","volume":"129","author":"Dawson","year":"1995","journal-title":"J. Cell Biol."},{"key":"10.1016\/S0076-6879(05)98016-6_bib0004","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1083\/jcb.153.1.121","article-title":"Cyclin A is destroyed in prometaphase and can delay chromosome alignment and anaphase","volume":"153","author":"den Elzen","year":"2001","journal-title":"J. Cell Biol."},{"key":"10.1016\/S0076-6879(05)98016-6_bib0005","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/0003-9861(59)90090-6","article-title":"Tissue sulfhydryl groups","volume":"82","author":"Ellman","year":"1959","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/S0076-6879(05)98016-6_bib0006","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S1097-2765(00)80126-4","article-title":"Direct binding of CDC20 protein family members activates the anaphase\u2010promoting complex in mitosis and G1","volume":"2","author":"Fang","year":"1998","journal-title":"Mol. Cell"},{"key":"10.1016\/S0076-6879(05)98016-6_bib0007","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1083\/jcb.153.1.137","article-title":"Anaphase\u2010promoting complex\/cyclosome\u2010dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint","volume":"153","author":"Geley","year":"2001","journal-title":"J. Cell Biol."},{"key":"10.1016\/S0076-6879(05)98016-6_bib0008","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/S1097-2765(01)00234-9","article-title":"Three\u2010dimensional structure of the anaphase\u2010promoting complex","volume":"7","author":"Gieffers","year":"2001","journal-title":"Mol. Cell"},{"key":"10.1016\/S0076-6879(05)98016-6_bib0009","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1038\/349132a0","article-title":"Cyclin is degraded by the ubiquitin pathway","volume":"349","author":"Glotzer","year":"1991","journal-title":"Nature"},{"key":"10.1016\/S0076-6879(05)98016-6_bib0010","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1083\/jcb.200111001","article-title":"Human securin proteolysis is controlled by the spindle checkpoint and reveals when the APC\/C switches from activation by Cdc20 to Cdh1","volume":"157","author":"Hagting","year":"2002","journal-title":"J. Cell Biol."},{"key":"10.1016\/S0076-6879(05)98016-6_bib9001","series-title":"Using antibodies: A laboratory manual","author":"Harlow","year":"1988"},{"key":"10.1016\/S0076-6879(05)98016-6_bib0011","doi-asserted-by":"crossref","first-page":"2179","DOI":"10.1101\/gad.1013102","article-title":"The anaphase\u2010promoting complex: It's not just for mitosis any more","volume":"16","author":"Harper","year":"2002","journal-title":"Genes Dev."},{"issue":"19","key":"10.1016\/S0076-6879(05)98016-6_bib0012","doi-asserted-by":"crossref","DOI":"10.1016\/S0076-6879(05)98019-1","article-title":"Identification of cell cycle dependent phosphorylation sites on the anaphase\u2010promoting complex\/cyclosome by mass spectrometry","volume":"398","author":"Herzog","year":"2005","journal-title":"Methods Enzymol."},{"key":"10.1016\/S0076-6879(05)98016-6_bib0013","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/0092-8674(95)90338-0","article-title":"A 20S complex containing CDC27 and CDC16 catalyzes the mitosis\u2010specific conjugation of ubiquitin to cyclin B","volume":"81","author":"King","year":"1995","journal-title":"Cell"},{"key":"10.1016\/S0076-6879(05)98016-6_bib0014","article-title":"Methods to measure ubiquitin\u2010dependent proteolysis mediated by the anaphase\u2010promoting complex (APC)","author":"Kraft","year":"2005","journal-title":"Methods"},{"key":"10.1016\/S0076-6879(05)98016-6_bib0015","doi-asserted-by":"crossref","first-page":"6598","DOI":"10.1093\/emboj\/cdg627","article-title":"Mitotic regulation of the human anaphase\u2010promoting complex by phosphorylation","volume":"22","author":"Kraft","year":"2003","journal-title":"EMBO J."},{"key":"10.1016\/S0076-6879(05)98016-6_bib0016","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1016\/S0960-9822(07)00510-6","article-title":"Activation of the human anaphase\u2010promoting complex by proteins of the CDC20\/Fizzy family","volume":"8","author":"Kramer","year":"1998","journal-title":"Curr. Biol."},{"key":"10.1016\/S0076-6879(05)98016-6_bib0017","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1091\/mbc.11.5.1555","article-title":"Mitotic regulation of the APC activator proteins CDC20 and CDH1","volume":"11","author":"Kramer","year":"2000","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/S0076-6879(05)98016-6_bib0018","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/S0091-679X(08)60298-8","article-title":"Cell cycle extracts","volume":"36","author":"Murray","year":"1991","journal-title":"Methods Cell Biol."},{"key":"10.1016\/S0076-6879(05)98016-6_bib0019","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1038\/nrm929","article-title":"The spindle checkpoint: Structural insights into dynamic signalling","volume":"3","author":"Musacchio","year":"2002","journal-title":"Nature Rev. Mol. Cell Biol."},{"key":"10.1016\/S0076-6879(05)98016-6_bib0020","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/S0092-8674(03)00034-5","article-title":"Structural basis for phosphodependent substrate selection and orientation by the SCFCdc4 ubiquitin ligase","volume":"112","author":"Orlicky","year":"2003","journal-title":"Cell"},{"key":"10.1016\/S0076-6879(05)98016-6_bib0021","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1016\/S1097-2765(02)00540-3","article-title":"The anaphase\u2010promoting complex: Proteolysis in mitosis and beyond","volume":"9","author":"Peters","year":"2002","journal-title":"Mol. Cell"},{"key":"10.1016\/S0076-6879(05)98016-6_bib0022","doi-asserted-by":"crossref","first-page":"3562","DOI":"10.1128\/MCB.24.8.3562-3576.2004","article-title":"Swm1\/Apc13 is an evolutionarily conserved subunit of the anaphase\u2010promoting complex stabilizing the association of Cdc16 and Cdc27","volume":"24","author":"Schwickart","year":"2004","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/S0076-6879(05)98016-6_bib0023","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1006\/bbrc.1999.0884","article-title":"Phosphorylation of the cyclosome is required for its stimulation by Fizzy\/cdc20","volume":"260","author":"Shteinberg","year":"1999","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/S0076-6879(05)98016-6_bib0024","doi-asserted-by":"crossref","first-page":"4827","DOI":"10.1002\/j.1460-2075.1995.tb00164.x","article-title":"Exit from mitosis is regulated by Drosophila fizzy and the sequential destruction of cyclins A, B and B3","volume":"14","author":"Sigrist","year":"1995","journal-title":"EMBO J."},{"key":"10.1016\/S0076-6879(05)98016-6_bib0025","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1091\/mbc.6.2.185","article-title":"The cyclosome, a large complex containing cyclin\u2010selective ubiquitin ligase activity, targets cyclins for destruction at the end of mitosis","volume":"6","author":"Sudakin","year":"1995","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/S0076-6879(05)98016-6_bib0026","doi-asserted-by":"crossref","first-page":"3839","DOI":"10.1091\/mbc.12.12.3839","article-title":"APC2 cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase\u2010promoting complex","volume":"12","author":"Tang","year":"2001","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/S0076-6879(05)98016-6_bib0027","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1126\/science.278.5337.460","article-title":"CDC20 and CDH1: A family of substrate\u2010specific activators of APC\u2010dependent proteolysis","volume":"278","author":"Visintin","year":"1997","journal-title":"Science"},{"key":"10.1016\/S0076-6879(05)98016-6_bib0028","doi-asserted-by":"crossref","first-page":"R834","DOI":"10.1016\/S0960-9822(01)00498-5","article-title":"Cell cycle: Waiters serving the Destruction machinery","volume":"11","author":"Vodermaier","year":"2001","journal-title":"Curr. Biol."},{"key":"10.1016\/S0076-6879(05)98016-6_bib0029","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1016\/S0960-9822(03)00581-5","article-title":"TPR subunits of the anaphase\u2010promoting complex mediate binding to the activator protein CDH1","volume":"13","author":"Vodermaier","year":"2003","journal-title":"Curr. Biol."},{"key":"10.1016\/S0076-6879(05)98016-6_bib0030","doi-asserted-by":"crossref","first-page":"4557","DOI":"10.1038\/sj.onc.1203803","article-title":"p55CDC\/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase","volume":"19","author":"Wu","year":"2000","journal-title":"Oncogene"},{"key":"10.1016\/S0076-6879(05)98016-6_bib0031","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0091-679X(08)60269-1","article-title":"Raising Xenopus in the laboratory","volume":"36","author":"Wu","year":"1991","journal-title":"Methods Cell Biol."},{"key":"10.1016\/S0076-6879(05)98016-6_bib0032","doi-asserted-by":"crossref","first-page":"18974","DOI":"10.1074\/jbc.M311060200","article-title":"Requirement of the SCFPop1\/Pop2 ubiquitin ligase for degradation of the fission yeast S phase cyclin Cig2","volume":"279","author":"Yamano","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0076-6879(05)98016-6_bib0033","doi-asserted-by":"crossref","first-page":"2048","DOI":"10.1016\/S0960-9822(02)01331-3","article-title":"Proteomics analysis identifies new components of the fission and budding yeast anaphase\u2010promoting complexes","volume":"12","author":"Yoon","year":"2002","journal-title":"Curr. Biol."}],"container-title":["Methods in Enzymology","Ubiquitin and Protein Degradation, Part A"],"original-title":[],"deposited":{"date-parts":[[2019,4,4]],"date-time":"2019-04-04T11:54:56Z","timestamp":1554378896000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0076687905980166"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005]]},"references-count":34,"URL":"https:\/\/doi.org\/10.1016\/s0076-6879(05)98016-6","relation":{},"ISSN":["0076-6879"],"issn-type":[{"value":"0076-6879","type":"print"}],"subject":[],"published":{"date-parts":[[2005]]}}}