{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T12:28:34Z","timestamp":1780403314653,"version":"3.54.1"},"reference-count":33,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[1998,3,1]],"date-time":"1998-03-01T00:00:00Z","timestamp":888710400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,8,17]],"date-time":"2013-08-17T00:00:00Z","timestamp":1376697600000},"content-version":"vor","delay-in-days":5648,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell"],"published-print":{"date-parts":[[1998,3]]},"DOI":"10.1016\/s0092-8674(00)81403-8","type":"journal-article","created":{"date-parts":[[2004,4,10]],"date-time":"2004-04-10T09:39:29Z","timestamp":1081589969000},"page":"747-758","source":"Crossref","is-referenced-by-count":344,"title":["BiP Maintains the Permeability Barrier of the ER Membrane by Sealing the Lumenal End of the Translocon Pore before and Early in Translocation"],"prefix":"10.1016","volume":"92","author":[{"given":"Brian D","family":"Hamman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda M","family":"Hendershot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur E","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/S0092-8674(00)81403-8_BIB1","doi-asserted-by":"crossref","first-page":"2123","DOI":"10.1126\/science.278.5346.2123","article-title":"Alignment of conduits for the nascent polypeptide chain in the ribosome-Sec61 complex","volume":"278","author":"Beckmann","year":"1997","journal-title":"Science"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB2","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1021\/bi9520848","article-title":"Crystal structure of diphtheria toxin bound to nicotinamide adenine dinucleotide","volume":"35","author":"Bell","year":"1996","journal-title":"Biochemistry"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB3","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1083\/jcb.123.6.1355","article-title":"A Sec63p\u2013BiP complex from yeast is required for protein translocation in a reconstituted proteoliposome","volume":"123","author":"Brodsky","year":"1993","journal-title":"J. Cell Biol."},{"key":"10.1016\/S0092-8674(00)81403-8_BIB4","doi-asserted-by":"crossref","first-page":"9643","DOI":"10.1073\/pnas.92.21.9643","article-title":"BiP and Sec63p are required for both co- and posttranslational protein translocation into the yeast endoplasmic reticulum","volume":"92","author":"Brodsky","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB5","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1038\/335649a0","article-title":"Defective co-translational formation of disulphide-isomerase-deficient microsomes","volume":"335","author":"Bulleid","year":"1988","journal-title":"Nature"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB6","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1083\/jcb.137.7.1483","article-title":"The lumenal domain of Sec63p stimulates the ATPase activity of BiP and mediates BiP recruitment to the translocon in Saccharomyces cerevisiae","volume":"137","author":"Corsi","year":"1997","journal-title":"J. Cell Biol."},{"key":"10.1016\/S0092-8674(00)81403-8_BIB7","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1016\/0092-8674(93)90640-C","article-title":"The signal sequence moves through a ribosomal tunnel into a noncytoplasmic aqueous environment at the ER membrane early in translocation","volume":"73","author":"Crowley","year":"1993","journal-title":"Cell"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB8","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/0092-8674(94)90424-3","article-title":"Secretory proteins move through the endoplasmic reticulum membrane via an aqueous, gated pore","volume":"78","author":"Crowley","year":"1994","journal-title":"Cell"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB9","doi-asserted-by":"crossref","first-page":"6381","DOI":"10.1074\/jbc.270.11.6381","article-title":"Autophosphorylation of grp94 (endoplasmin)","volume":"270","author":"Csermely","year":"1995","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0092-8674(00)81403-8_BIB10","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/S0092-8674(00)81115-0","article-title":"The cotranslational integration of membrane proteins into the phospholipid bilayer is a multistep process","volume":"85","author":"Do","year":"1996","journal-title":"Cell"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB11","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1038\/346623a0","article-title":"Three-dimensional structure of the ATPase fragment of a 70K heat-shock cognate protein","volume":"346","author":"Flaherty","year":"1990","journal-title":"Nature"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB12","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/355033a0","article-title":"Protein folding in the cell","volume":"355","author":"Gething","year":"1992","journal-title":"Nature"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB13","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/S0092-8674(00)80235-4","article-title":"The aqueous pore through the translocon has a diameter of 40\u201360 \u00c5 during cotranslational protein translocation at the ER membrane","volume":"89","author":"Hamman","year":"1997","journal-title":"Cell"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB14","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/S0092-8674(00)81391-4","article-title":"Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation","volume":"87","author":"Hanein","year":"1996","journal-title":"Cell"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB15","doi-asserted-by":"crossref","first-page":"5269","DOI":"10.1073\/pnas.93.11.5269","article-title":"Inhibition of immunoglobulin folding and secretion by dominant negative BiP ATPase mutants","volume":"93","author":"Hendershot","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB16","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/S0962-8924(97)01029-5","article-title":"Protein translocation at the ER membrane","volume":"7","author":"Johnson","year":"1997","journal-title":"Trends Cell Biol."},{"key":"10.1016\/S0092-8674(00)81403-8_BIB17","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/0092-8674(95)90313-5","article-title":"A posttargeting signal sequence recognition event in the endoplasmic reticulum membrane","volume":"82","author":"Jungnickel","year":"1995","journal-title":"Cell"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB18","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1083\/jcb.126.4.925","article-title":"Binding of ribosomes to the rough endoplasmic reticulum mediated by the Sec61p-complex","volume":"126","author":"Kalies","year":"1994","journal-title":"J. Cell Biol."},{"key":"10.1016\/S0092-8674(00)81403-8_BIB19","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0092-8674(00)80311-6","article-title":"Both lumenal and cytosolic gating of the aqueous ER translocon pore are regulated from inside the ribosome during membrane protein integration","volume":"90","author":"Liao","year":"1997","journal-title":"Cell"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB20","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0092-8674(00)81861-9","article-title":"Binding of secretory precursor polypeptides to a translocon subcomplex is regulated by BiP","volume":"88","author":"Lyman","year":"1997","journal-title":"Cell"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB21","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1083\/jcb.131.6.1377","article-title":"Sac1 mediates the adenosine triphoshate transport into yeast endoplasmic reticulum that is required for protein translocation","volume":"131","author":"Mayinger","year":"1995","journal-title":"J. Cell Biol."},{"key":"10.1016\/S0092-8674(00)81403-8_BIB22","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1016\/0092-8674(93)90276-V","article-title":"Lumenal proteins of the mammalian endoplasmic reticulum are required to complete protein translocation","volume":"73","author":"Nicchitta","year":"1993","journal-title":"Cell"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB23","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1073\/pnas.88.4.1565","article-title":"Binding protein BiP is required for translocation of secretory proteins into the endoplasmic reticulum in Saccharomyces cerevisiae","volume":"88","author":"Nguyen","year":"1991","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB24","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/0092-8674(95)90077-2","article-title":"Posttranslational protein transport in yeast reconstituted with a purified complex of Sec proteins and Kar2p","volume":"81","author":"Panzner","year":"1995","journal-title":"Cell"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB25","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1042\/bj2570657","article-title":"Preparation and characterization of dog pancreas microsomal membranes specifically depleted of protein disulfide isomerase","volume":"257","author":"Paver","year":"1989","journal-title":"Biochem. J."},{"key":"10.1016\/S0092-8674(00)81403-8_BIB26","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/S0960-9822(02)00484-0","article-title":"The nascent polypeptide-associated complex modulates interactions between the signal recognition particle and the ribosome","volume":"6","author":"Powers","year":"1996","journal-title":"Curr. Biol."},{"key":"10.1016\/S0092-8674(00)81403-8_BIB27","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1146\/annurev.bi.65.070196.001415","article-title":"Protein translocation across the eukaryotic endoplasmic reticulum and bacterial inner membranes","volume":"65","author":"Rapoport","year":"1996","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/S0092-8674(00)81403-8_BIB28","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1006\/prep.1994.1049","article-title":"A single purification procedure for the major resident proteins of the ER lumen","volume":"5","author":"Rowling","year":"1994","journal-title":"Protein Expression Purif."},{"key":"10.1016\/S0092-8674(00)81403-8_BIB29","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/0092-8674(92)90415-9","article-title":"Sec61p and BiP directly facilitate polypeptide translocation into the ER","volume":"69","author":"Sanders","year":"1992","journal-title":"Cell"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB30","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/0092-8674(91)90455-8","article-title":"A protein-conducting channel in the endoplasmic reticulum","volume":"65","author":"Simon","year":"1991","journal-title":"Cell"},{"key":"10.1016\/S0092-8674(00)81403-8_BIB31","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1083\/jcb.130.1.41","article-title":"BiP\/Kar2p serves as a molecular chaperone during carboxypeptidase Y folding in yeast","volume":"130","author":"Simons","year":"1995","journal-title":"J. Cell Biol."},{"key":"10.1016\/S0092-8674(00)81403-8_BIB32","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.1083\/jcb.110.6.1885","article-title":"Loss of BiP\/GRP78 function blocks translocation of secretory proteins in yeast","volume":"110","author":"Vogel","year":"1990","journal-title":"J. Cell Biol."},{"key":"10.1016\/S0092-8674(00)81403-8_BIB33","doi-asserted-by":"crossref","first-page":"26670","DOI":"10.1074\/jbc.270.44.26670","article-title":"Characterization of the nucleotide binding properties and ATPase activity of recombinant hamster BiP purified from bacteria","volume":"270","author":"Wei","year":"1995","journal-title":"J. Biol. Chem."}],"container-title":["Cell"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0092867400814038?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0092867400814038?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,2,14]],"date-time":"2019-02-14T05:58:50Z","timestamp":1550123930000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0092867400814038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,3]]},"references-count":33,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1998,3]]}},"alternative-id":["S0092867400814038"],"URL":"https:\/\/doi.org\/10.1016\/s0092-8674(00)81403-8","relation":{},"ISSN":["0092-8674"],"issn-type":[{"value":"0092-8674","type":"print"}],"subject":[],"published":{"date-parts":[[1998,3]]}}}