{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:30:49Z","timestamp":1773657049115,"version":"3.50.1"},"reference-count":34,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[1996,12,1]],"date-time":"1996-12-01T00:00:00Z","timestamp":849398400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gene"],"published-print":{"date-parts":[[1996,12]]},"DOI":"10.1016\/s0378-1119(96)00535-5","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T18:13:42Z","timestamp":1027620822000},"page":"137-143","source":"Crossref","is-referenced-by-count":18,"title":["Isolation, sequencing and overproduction of the single-stranded DNA binding protein from Pseudomonas aeruginosa PAO"],"prefix":"10.1016","volume":"182","author":[{"given":"Jochen","family":"Genschel","sequence":"first","affiliation":[]},{"given":"Lidia","family":"Litz","sequence":"additional","affiliation":[]},{"given":"Hubert","family":"Thole","sequence":"additional","affiliation":[]},{"given":"Ute","family":"Roemling","sequence":"additional","affiliation":[]},{"given":"Claus","family":"Urbanke","sequence":"additional","affiliation":[]}],"member":"78","reference":[{"key":"10.1016\/S0378-1119(96)00535-5_BIB1","doi-asserted-by":"crossref","first-page":"2587","DOI":"10.1093\/nar\/19.10.2587","article-title":"Influence of the incorporation of (S)-9-(3,4-dihydroxybutyl)adenine on the enzymatic stability and base-pairing properties of oligodeoxynucleotides","volume":"19","author":"Augustyns","year":"1991","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB2","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0014-5793(85)81128-5","article-title":"Variability in the nucleic acid binding site size and the amount of single-stranded DNA-binding protein in Escherichia coli","volume":"181","author":"Bobst","year":"1985","journal-title":"FEBS Lett."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB3","doi-asserted-by":"crossref","first-page":"5095","DOI":"10.1093\/nar\/13.14.5095","article-title":"Analysis of the regulatory region of the ssb gene of Escherichia coli","volume":"13","author":"Brandsma","year":"1985","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB4","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/0003-2697(74)90360-1","article-title":"Tryptophan analysis of proteins in 6 M guanidine hydrochloride: Modification for more general application","volume":"61","author":"Bredderman","year":"1974","journal-title":"Anal. Biochem."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB5","first-page":"669","article-title":"High level production of biologically active human \u00c2(1) antitrypsin in E. coli","volume":"81","author":"Courtney","year":"1984"},{"key":"10.1016\/S0378-1119(96)00535-5_BIB6","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1111\/j.1432-1033.1994.tb18756.x","article-title":"Single-stranded-DNA-binding proteins from human mitochondria and Escherichia coli have analogous physicochemical properties","volume":"221","author":"Curth","year":"1994","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB7","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/0378-1119(93)90614-9","article-title":"Cloning and sequencing of the Serratia marcescens gene encoding a single-stranded DNA binding protein (SSB) and its promoter region","volume":"127","author":"de Vries","year":"1993","journal-title":"Gene"},{"key":"10.1016\/S0378-1119(96)00535-5_BIB8","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1099\/00221287-140-4-889","article-title":"Cloning and sequencing of the Proteus mirabilis gene for a single-stranded DNA binding protein (SSB) and complementation of Escherichia coli ssb point and deletion mutations","volume":"14","author":"de Vries","year":"1994","journal-title":"Microbiology"},{"key":"10.1016\/S0378-1119(96)00535-5_BIB9","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1111\/j.1432-1033.1994.00613.x","article-title":"The single-stranded DNA-binding proteins (SSB) of Proteus mirabilis and Serratia marcescens","volume":"224","author":"de Vries","year":"1994","journal-title":"Eur. J. Biochem."},{"issue":"1","key":"10.1016\/S0378-1119(96)00535-5_BIB10","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1128\/JB.140.1.14-19.1979","article-title":"Mutant single-strand binding protein of Escherichia coli: genetic and physiological characterization","volume":"140","author":"Glassberg","year":"1979","journal-title":"J. Bacteriol."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB11","series-title":"Protein-Nucleic Acid Interaction","first-page":"61","article-title":"The single-stranded DNA binding protein of Escherichia coli. Physicochemical properties and biological functions","author":"Greipel","year":"1989"},{"key":"10.1016\/S0378-1119(96)00535-5_BIB12","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1146\/annurev.mi.40.100186.000455","article-title":"Genome organization in Pseudomonads","volume":"40","author":"Holloway","year":"1986","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB13","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/0378-1119(94)90841-9","article-title":"Cloning and sequencing of the Haemophilus influenzae ssb gene encoding single-stranded DNA-binding protein","volume":"146","author":"Jarosik","year":"1994","journal-title":"Gene"},{"key":"10.1016\/S0378-1119(96)00535-5_BIB14","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/BF00268691","article-title":"Genetic mapping of the lexC-113 mutation","volume":"157","author":"Johnson","year":"1977","journal-title":"Mol. Gen. Genet."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB15","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1146\/annurev.bi.63.070194.002523","article-title":"Escherichia coli single-stranded DNA binding protein: Multiple DNA-binding modes and cooperativities","volume":"63","author":"Lohman","year":"1994","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB16","doi-asserted-by":"crossref","first-page":"3594","DOI":"10.1016\/S0021-9258(19)83663-3","article-title":"Two binding modes in E. coli single-strand binding protein (SSB) single-stranded DNA complexes: Modulation by NaCl concentration","volume":"260","author":"Lohman","year":"1985","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB17","series-title":"Expression of the ssb gene under lambda PL control","first-page":"1","article-title":"Large-scale overproduction and rapid purification of the E.coli ssb gene product","volume":"25","author":"Lohman","year":"1986"},{"key":"10.1016\/S0378-1119(96)00535-5_BIB18","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1128\/MR.54.4.342-380.1990","article-title":"The single stranded DNA binding protein of E. coli","volume":"54","author":"Meyer","year":"1990","journal-title":"Microbiol. Rev."},{"issue":"1","key":"10.1016\/S0378-1119(96)00535-5_BIB19","first-page":"19","article-title":"Resolution of common DNA sequencing ambiguities of G\/C-rich DNA-templates by terminal deoxynucleotidyl transferase without dGTP analogues","volume":"15","author":"Ming","year":"1993","journal-title":"Focus"},{"key":"10.1016\/S0378-1119(96)00535-5_BIB20","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1016\/S0022-2836(75)80012-X","article-title":"Properties of the Escherichia coli DNA binding (unwinding) protein: Interaction with nucleolytic enzymes and DNA","volume":"98","author":"Molineux","year":"1975","journal-title":"J. Mol. Biol."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/dnares\/1.1.1","article-title":"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin","volume":"1","author":"Ogasawara","year":"1994","journal-title":"DNA Res."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB22","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1038\/nbt0190-47","article-title":"Use of the Escherichia coli ssb gene to prevent bioreactor takeover by plasmid-less cells","volume":"8","author":"Porter","year":"1990","journal-title":"BioTechnology"},{"key":"10.1016\/S0378-1119(96)00535-5_BIB23","doi-asserted-by":"crossref","first-page":"3199","DOI":"10.1093\/nar\/19.12.3199","article-title":"The impact of two-dimensional pulsed field electrophoresis techniques for the consistent and complete mapping of bacterial genomes: refined physical map of Pseudomonas aeruginosa PAO","volume":"19","author":"Roemling","year":"1991","journal-title":"Nucleic Acid Res."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB24","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1146\/annurev.ge.13.120179.001535","article-title":"Regulatory sequences involved in the promotion and termination of RNA transcription","volume":"13","author":"Rosenberg","year":"1979","journal-title":"Annu. Rev. Genet."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB25","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1002\/prot.340090206","article-title":"Single-stranded DNA binding proteins (SSBs) from procaryotic transmissible plasmids","volume":"9","author":"Ruvolo","year":"1991","journal-title":"Proteins"},{"key":"10.1016\/S0378-1119(96)00535-5_BIB26","first-page":"4274","article-title":"Sequences of the ssb gene and protein","volume":"78","author":"Sancar","year":"1981"},{"key":"10.1016\/S0378-1119(96)00535-5_BIB27","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/0022-2836(75)90213-2","article-title":"A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase","volume":"94","author":"Sanger","year":"1975","journal-title":"J. Mol. Biol."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB28","doi-asserted-by":"crossref","first-page":"4378","DOI":"10.1128\/jb.172.8.4378-4385.1990","article-title":"Signal strains that can detect certain DNA replication and membrane mutants of Escherichia coli: Isolation of a new ssb allele, ssb-3","volume":"172","author":"Schmellik-Sandage","year":"1990","journal-title":"J. Bacteriol."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB29","series-title":"Physico-chemical Investigations","article-title":"Interactions of the Single-Stranded DNA Binding Protein from Escherichia coli and Nucleic Acids","author":"Sindermann","year":"1981"},{"key":"10.1016\/S0378-1119(96)00535-5_BIB30","doi-asserted-by":"crossref","first-page":"30005","DOI":"10.1016\/S0021-9258(18)43981-6","article-title":"Protein interactions in genetic recombination in Escherichia coli","volume":"269","author":"Umezu","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB31","doi-asserted-by":"crossref","first-page":"3450","DOI":"10.1093\/nar\/22.17.3450","article-title":"ECD-a totally integrated database of Escherichia coli K12","volume":"22","author":"Wahl","year":"1994","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB32","doi-asserted-by":"crossref","first-page":"1972","DOI":"10.1016\/S0021-9258(19)41671-2","article-title":"The deoxyribo-nucleic acid unwinding protein of Escherichia coli","volume":"250","author":"Weiner","year":"1975","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB33","doi-asserted-by":"crossref","first-page":"3346","DOI":"10.1016\/S0021-9258(18)32867-9","article-title":"Limited proteolysis studies on the E. coli single-stranded DNA binding protein","volume":"258","author":"Williams","year":"1983","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0378-1119(96)00535-5_BIB34","doi-asserted-by":"crossref","first-page":"5339","DOI":"10.1128\/IAI.61.12.5339-5344.1993","article-title":"Isolation of Brucella abortus ssb and uvrA genes from a genomic library by use of lymphocytes as probes","volume":"61","author":"Zhu","year":"1993","journal-title":"Infect. Immun."}],"container-title":["Gene"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378111996005355?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378111996005355?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,5,9]],"date-time":"2021-05-09T15:49:10Z","timestamp":1620575350000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378111996005355"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,12]]},"references-count":34,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[1996,12]]}},"alternative-id":["S0378111996005355"],"URL":"https:\/\/doi.org\/10.1016\/s0378-1119(96)00535-5","relation":{},"ISSN":["0378-1119"],"issn-type":[{"value":"0378-1119","type":"print"}],"subject":[],"published":{"date-parts":[[1996,12]]}}}