{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T11:19:53Z","timestamp":1781522393296,"version":"3.54.1"},"reference-count":43,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[1998,4,1]],"date-time":"1998-04-01T00:00:00Z","timestamp":891388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gene"],"published-print":{"date-parts":[[1998,4]]},"DOI":"10.1016\/s0378-1119(98)00081-x","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T12:25:34Z","timestamp":1027599934000},"page":"39-47","source":"Crossref","is-referenced-by-count":55,"title":["Properties of exgS, a gene for a major subunit of the Clostridium cellulovorans cellulosome"],"prefix":"10.1016","volume":"211","author":[{"given":"Chi-Chi","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roy H","family":"Doi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/S0378-1119(98)00081-X_BIB1","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/0378-1119(92)90062-T","article-title":"Sequence analysis of a gene cluster encoding cellulases from Clostridium cellulolyticum","volume":"119","author":"Bagnara-Tardif","year":"1992","journal-title":"Gene"},{"key":"10.1016\/S0378-1119(98)00081-X_BIB2","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1128\/JB.162.1.102-105.1985","article-title":"Sequence of a cellulase gene of the thermophilic bacterium Clostridium thermocellum","volume":"162","author":"Beguin","year":"1985","journal-title":"J. Bacteriol."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB3","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1111\/j.1574-6976.1994.tb00033.x","article-title":"The biological degradation of cellulose","volume":"13","author":"Beguin","year":"1994","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB4","unstructured":"Belaich, J.P., Gaudin, C., Belaich, A., Bagnara-Tardif, C., Fierobe, H.P., Reverbel, C., 1994. Molecular organization and biochemistry of the cellulolytic system of Clostridium cellulolyticum. In: Shimada, K., Hoshino, S., Ohmiya, K., Sakka, K., Kobayashi, Y., Karita, S. (Eds.), Genetics, Biochemistry and Ecology of Lignocellulose Degradation. Uni Publishers, Tokyo, pp. 53\u201362."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB5","doi-asserted-by":"crossref","first-page":"87","DOI":"10.3109\/10408419409113548","article-title":"The Clostridium cellulovorans cellulosome","volume":"20","author":"Doi","year":"1994","journal-title":"CRC Rev. Microbiol."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB6","unstructured":"Doi, R.H., Goldstein, M., Park, J.S., Liu, C.C., Matano, Y., Takagi, M., Hashida, S., Foong, F.C.F., Hamamoto, T., Segel, I., Shoseyov, O., 1994b. Structure and function of the subunits of the Clostridium cellulovorans cellulosome. In: Shimada, K., Hoshino, S., Ohmiya, K., Sakka, K., Kobayashi, Y., Karita, S. (Eds.), Genetics, Biochemistry and Ecology of Lignocellulose Degradation. Uni Publishers, Tokyo, pp. 43\u201352."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB7","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/0378-1119(89)90137-6","article-title":"Sequence analysis of the Clostridium cellulolyticum endoglucanase A encoding gene, celCCA","volume":"84","author":"Faure","year":"1989","journal-title":"Gene"},{"key":"10.1016\/S0378-1119(98)00081-X_BIB8","doi-asserted-by":"crossref","first-page":"1729","DOI":"10.1099\/00221287-137-7-1729","article-title":"Nucleotide sequence and characteristics of endoglucanase gene engB from Clostridium cellulovorans","volume":"137","author":"Foong","year":"1991","journal-title":"J. Gen. Microbiol."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB9","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1128\/jb.174.4.1403-1409.1992","article-title":"Characterization and comparison of Clostridium cellulovorans endoglucanases\u2013xylanases EngB and EngD hyperexpressed in Escherichia coli","volume":"174","author":"Foong","year":"1992","journal-title":"J. Bacteriol."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB10","doi-asserted-by":"crossref","first-page":"1891","DOI":"10.1128\/jb.175.7.1891-1899.1993","article-title":"Organization of a Clostridium thermocellum gene cluster encoding the cellulosomal scaffolding protein cipA and a protein possibly involved in attachment of the cellulosome to the cell surface","volume":"175","author":"Fujino","year":"1993","journal-title":"J. Bacteriol."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB11","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/0922-338X(93)90188-E","article-title":"Nucleotide sequences of the celB gene encoding endo-1,4-\u03b2-glucanase-2, ORF1, and ORF2 forming a putative cellulase gene cluster of Clostridium josui","volume":"76","author":"Fujino","year":"1993","journal-title":"J. Ferment. Bioeng."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB12","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1093\/nar\/14.4.1791","article-title":"Sequence of the cellulase gene of Clostridium thermocellum coding for endoglucanase B","volume":"14","author":"Grepinet","year":"1986","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB13","doi-asserted-by":"crossref","first-page":"4582","DOI":"10.1128\/jb.170.10.4582-4588.1988","article-title":"Nucleotide sequence and deletion analysis of the xylanase gene (xynZ) of Clostridium thermocellum","volume":"170","author":"Grepinet","year":"1988","journal-title":"J. Bacteriol."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB14","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/0378-1119(88)90375-7","article-title":"Conserved reiterated domains in Clostridium thermocellum endoglucanases are not essential for catalytic activity","volume":"69","author":"Hall","year":"1988","journal-title":"Gene"},{"key":"10.1016\/S0378-1119(98)00081-X_BIB15","unstructured":"Halliwell, G., Halliwell, N., 1984. Cellulose. In: Halliwell, G., Halliwell, N. (Eds.), Methods of Enzymatic Analysis, Academic Press, New York, pp. 18\u201341."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB16","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1007\/BF00292718","article-title":"Analysis of functional domains of endoglucanases from Clostridium cellulovorans by gene cloning, nucleotide sequencing and chimeric protein construction","volume":"231","author":"Hamamoto","year":"1991","journal-title":"Mol. Gen. Genet."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB17","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1042\/bj3160695","article-title":"Updating the sequence based classification of glycosyl hydrolases","volume":"316","author":"Henrissat","year":"1996","journal-title":"Biochem. J."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB18","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1007\/BF00265062","article-title":"Sequence analysis of the Clostridium stercorarium celZ gene encoding a thermoactive cellulase (Avicelase I): identification of catalytic and cellulose binding domains","volume":"223","author":"Jauris","year":"1990","journal-title":"Mol. Gen. Genet."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB19","doi-asserted-by":"crossref","first-page":"8605","DOI":"10.1093\/nar\/14.21.8605","article-title":"Nucleotide sequence of the cellulase gene celD encoding endoglucanase D of Clostridium thermocellum","volume":"14","author":"Joliff","year":"1986","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB20","doi-asserted-by":"crossref","first-page":"1641","DOI":"10.1128\/jb.177.6.1641-1644.1995","article-title":"Exoglucanase activities of the recombinant Clostridium thermocellum CelS, a major cellulosome component","volume":"177","author":"Kruus","year":"1995","journal-title":"J. Bacteriol."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB21","unstructured":"Lamed, R., Bayer, E.A., 1988. The cellulosome concept: exocellular\/extracellular enzyme reactor centers for efficient binding and cellulolysis. In: Aubert, J.-P., Beguin, P., Millet, J. (Eds.), Biochemistry and Genetics of Cellulose Degradation. Academic Press, San Diego, CA, pp. 101\u2013116."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB22","doi-asserted-by":"crossref","first-page":"3353","DOI":"10.1128\/jb.175.11.3353-3360.1993","article-title":"Nucleotide sequence of the celG gene of Clostridium thermocellum and characterizaton of its product, endoglucanase CelG","volume":"175","author":"Lemaire","year":"1993","journal-title":"J. Bacteriol."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB23","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1128\/AEM.57.3.694-700.1991","article-title":"Cloning, sequence analysis, and expression in Escherichia coli of a gene coding for a \u03b2-mannanase from the extremely thermophilic bacterium Caldocellum saccharolyticum","volume":"57","author":"Luthi","year":"1991","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB24","doi-asserted-by":"crossref","first-page":"4155","DOI":"10.1128\/jb.173.13.4155-4162.1991","article-title":"Isolation and properties of a major cellobiohydrolase from the cellulosome of Clostridium thermocellum","volume":"173","author":"Morag","year":"1991","journal-title":"J. Bacteriol."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB25","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1016\/0923-2508(91)90002-R","article-title":"Nucleotide sequence of the cellulase gene celF of Clostridium thermocellum","volume":"142","author":"Navarro","year":"1991","journal-title":"Res. Microbiol."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB26","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0021-9258(18)56635-7","article-title":"A submicrodetermination of glucose","volume":"181","author":"Park","year":"1949","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB27","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1099\/00221287-142-4-1013","article-title":"Molecular study and overexpression of the Clostridium cellulolyticum celF cellulase gene in Escherichia coli","volume":"142","author":"Reverbel-Leroy","year":"1996","journal-title":"Microbiology-UK"},{"key":"10.1016\/S0378-1119(98)00081-X_BIB28","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1128\/jb.179.1.46-52.1997","article-title":"The processive endocellulase CelF, a major component of the Clostridium cellulolyticum cellulosome: purification and characterization of the recombinant form","volume":"179","author":"Reverbel-Leroy","year":"1997","journal-title":"J. Bacteriol"},{"key":"10.1016\/S0378-1119(98)00081-X_BIB29","unstructured":"Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB30","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0378-1119(96)00544-6","article-title":"Characterization of engF, a gene for a non-cellulosomal Clostridium cellulovorans endoglucanase","volume":"182","author":"Sheweita","year":"1996","journal-title":"Gene"},{"key":"10.1016\/S0378-1119(98)00081-X_BIB31","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0378-1119(91)90461-J","article-title":"Nucleotide sequence analysis of the endoglucanase encoding gene, celCCD, of Clostridium cellulolyticum","volume":"104","author":"Shima","year":"1991","journal-title":"Gene"},{"key":"10.1016\/S0378-1119(98)00081-X_BIB32","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1111\/j.1432-1033.1992.tb16600.x","article-title":"Structure of the Clostridium thermocellum gene licB and the encoded \u03b2- 1,3-1,4-glucanase","volume":"204","author":"Shimming","year":"1992","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB33","doi-asserted-by":"crossref","first-page":"2192","DOI":"10.1073\/pnas.87.6.2192","article-title":"Essential 170kDa subunit for degradation of crystalline cellulose by Clostridium cellulovorans cellulase","volume":"87","author":"Shoseyov","year":"1990","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/S0378-1119(98)00081-X_BIB34","doi-asserted-by":"crossref","first-page":"3483","DOI":"10.1073\/pnas.89.8.3483","article-title":"Primary sequence analysis of Clostridium cellulovorans cellulose binding protein A (CbpA)","volume":"89","author":"Shoseyov","year":"1992","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/S0378-1119(98)00081-X_BIB35","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1128\/MR.57.1.109-137.1993","article-title":"Protein secretion in Bacillus species","volume":"57","author":"Simonen","year":"1993","journal-title":"Microbiol. Rev."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB36","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/0022-2836(86)90385-2","article-title":"Use of bacteriophage T7 RNA polymerase to direct selective high level expression of cloned genes","volume":"189","author":"Studier","year":"1986","journal-title":"J. Mol. Biol."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB37","doi-asserted-by":"crossref","first-page":"7119","DOI":"10.1128\/jb.175.21.7119-7122.1993","article-title":"The hydrophobic repeated domain of the Clostridium cellulovorans cellulose binding protein (CbpA) has specific interactions with endoglucanases","volume":"175","author":"Takagi","year":"1993","journal-title":"J. Bacteriol."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB38","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/BF00172827","article-title":"celA, another gene coding for a multidomain cellulase from the extreme thermophile Caldocellum saccharolyticum","volume":"43","author":"Te'o","year":"1995","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB39","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/0014-5793(91)81279-H","article-title":"Interaction of the duplicated segment carried by Clostridium thermocellum cellulases with cellulosome components","volume":"291","author":"Tokatlidis","year":"1991","journal-title":"FEBS Lett."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB40","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1093\/protein\/6.8.947","article-title":"Properties conferred on Clostridium thermocellum endoglucanase CelC by grafting the duplicated segment of endoglucanase CelD","volume":"6","author":"Tokatlidis","year":"1993","journal-title":"Prot. Eng."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB41","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1128\/jb.175.5.1293-1302.1993","article-title":"Cloning and DNA sequence of the gene coding for Clostridium thermocellum cellulase SS (CelS), a major cellulosome component","volume":"175","author":"Wang","year":"1993","journal-title":"J. Bacteriol."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB42","first-page":"346","article-title":"Cloning and expression of the Clostridium thermocellum celS gene in Escherichia coli","volume":"42","author":"Wang","year":"1994","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/S0378-1119(98)00081-X_BIB43","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/0378-1119(90)90206-7","article-title":"Nucleotide sequence and deletion analysis of the cellulase-encoding gene celH of Clostridium thermocellum","volume":"89","author":"Yague","year":"1990","journal-title":"Gene"}],"container-title":["Gene"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S037811199800081X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S037811199800081X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,5,5]],"date-time":"2021-05-05T18:24:00Z","timestamp":1620239040000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S037811199800081X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,4]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1998,4]]}},"alternative-id":["S037811199800081X"],"URL":"https:\/\/doi.org\/10.1016\/s0378-1119(98)00081-x","relation":{},"ISSN":["0378-1119"],"issn-type":[{"value":"0378-1119","type":"print"}],"subject":[],"published":{"date-parts":[[1998,4]]}}}