{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T09:49:53Z","timestamp":1769161793614,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,1,1]],"date-time":"2006-01-01T00:00:00Z","timestamp":1136073600000},"content-version":"vor","delay-in-days":1280,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal Orthopaedic Research"],"published-print":{"date-parts":[[2002,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Static and dynamic compression are known to modulate the metabolism of articular cartilage. The present study focused on determining the effects of compressive loading on the metabolism of sulfated glycosaminoglycans (S\u2010GAG) and protein in tissue engineered cartilage constructs. Cartilage constructs were subjected to static or dynamic compression for 24 h and radiolabeled with <jats:sup>35<\/jats:sup>SO<jats:sub>4<\/jats:sub> and <jats:sup>3<\/jats:sup>H\u2010proline to assess the total synthesis and percentage retention of S\u2010GAG and total protein, respectively. Static compression at an amplitude of 50% suppressed the synthesis of both S\u2010GAG and protein by 35% and 57%, respectively. Dynamic compression at an amplitude of 5% had stimulatory effects on synthesis that were dependent on the static offset compression amplitude (10% or 50%) and dynamic compression frequency (0.001 or 0.1 Hz). Thus, tissue engineered cartilage demonstrated the ability to respond to mechanical loading in a manner similar to that observed with articular cartilage. Mechanical loading may therefore potentially be used to modulate the growth of cartilaginous tissues in vitro, potentially facilitating the culture of functional cartilage tissues suitable for implantation. \u00a9 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.<\/jats:p>","DOI":"10.1016\/s0736-0266(01)00160-7","type":"journal-article","created":{"date-parts":[[2002,10,14]],"date-time":"2002-10-14T20:05:35Z","timestamp":1034625935000},"page":"842-848","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":212,"title":["Static and dynamic compression modulate matrix metabolism in tissue engineered cartilage"],"prefix":"10.1002","volume":"20","author":[{"given":"Twana","family":"Davisson","sequence":"first","affiliation":[]},{"given":"Sabine","family":"Kunig","sequence":"additional","affiliation":[]},{"given":"Albert","family":"Chen","sequence":"additional","affiliation":[]},{"given":"Robert","family":"Sah","sequence":"additional","affiliation":[]},{"given":"Anthony","family":"Ratcliffe","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,1]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9290(95)00103-4"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100110514"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.108.4.1497"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1999.1197"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19990120)62:2<166::AID-BIT6>3.0.CO;2-K"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19991105)65:3<274::AID-BIT4>3.0.CO;2-E"},{"key":"e_1_2_1_8_2","unstructured":"ChenAC BaeWC SchinaglRM SahRL. Depth\u2010 and strain\u2010dependent mechanical and electromechanical properties of fullthickness articular cartilage.J Biomech2000."},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100170612"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100160504"},{"key":"e_1_2_1_11_2","first-page":"199","article-title":"Composition\u2010function relationships for tissue engineered cartilage","volume":"25","author":"Davisson T","year":"2000","journal-title":"Trans Orthop Res Soc"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9290(87)90283-1"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1998.4010"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-199910001-00006"},{"key":"e_1_2_1_15_2","unstructured":"FreedLE Vunjak\u2010NovakovicG. Tissue engineering of cartilage. In: Bronzino J editor.The Biomedical Engineering Handbook 1995. p.1788\u2013806."},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100060602"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1063-4584(05)80059-7"},{"key":"e_1_2_1_18_2","unstructured":"GuilakF SahR SettonL.Physical regulation of cartilage metabolism. In: Mow V Hayes W editors. Basic Orthopaedic Biomechanics 1997. p.179\u2013207."},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100090102"},{"key":"e_1_2_1_20_2","unstructured":"HelminenH JurvelinJ KivirantaI PaukkonenK SaamanenA TammiM.Joint loading effects on articular cartilage: a historical review. In: Helminen H Kiviranta I Saamanen A et al. editors. Joint Loading 1987. p.1\u201346."},{"key":"e_1_2_1_21_2","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1097\/00003086-198205000-00043","article-title":"The effect of continuous mechanical pressure upon the turnover of articular cartilage proteoglycans in vitro","volume":"165","author":"Jones IL","year":"1982","journal-title":"Clin Orthop"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1996.0181"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1994.1201"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(88)90532-5"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0934-8832(11)80193-9"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100150205"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.3109\/03008208509152388"},{"key":"e_1_2_1_28_2","unstructured":"MartinI ObradovicB TreppoS GrodzinskyAJ LangerR FreedLE et al.Modulation of the mechanical properties of tissue engineered cartilage. Biorheology2000;37."},{"key":"e_1_2_1_29_2","unstructured":"MauckRL WongDD SoltzMA SeruyaM AteshianGA ValhumaWB et al.Characterization of alginate constructs for articular cartilage tissue engineering. In: 46th Annual Meeting. Orthopaedic Research Society;2000. p.200."},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780270611"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1993.1062"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(91)90004-3"},{"key":"e_1_2_1_33_2","unstructured":"SahR GrodzinskyA PlassA SandyJ. Effects of static and dynamic compression on matrix metabolism in cartilage explants. In: Kuettner K editor.Articular Cartilage and Osteoarthritis 1992. p.373\u201392."},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100070502"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1999.tb08522.x"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.7641"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1053\/joca.1998.0204"},{"key":"e_1_2_1_38_2","doi-asserted-by":"crossref","unstructured":"ValhmuWB StazzoneEJ BachrachNM Saed\u2010NejadF FischerSG MowVC et al. Load\u2010controlled compression of articular cartilage induces a transient stimulation of aggrecan gene expression.Arch Biochem Biophys1998;353.","DOI":"10.1006\/abbi.1998.0633"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100170119"}],"container-title":["Journal of Orthopaedic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2FS0736-0266(01)00160-7","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/S0736-0266%2801%2900160-7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T12:38:27Z","timestamp":1696682307000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1016\/S0736-0266%2801%2900160-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,7]]},"references-count":38,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,7]]}},"alternative-id":["10.1016\/S0736-0266(01)00160-7"],"URL":"https:\/\/doi.org\/10.1016\/s0736-0266(01)00160-7","archive":["Portico"],"relation":{},"ISSN":["0736-0266","1554-527X"],"issn-type":[{"value":"0736-0266","type":"print"},{"value":"1554-527X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,7]]},"assertion":[{"value":"2001-10-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}