{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T13:49:32Z","timestamp":1770472172004,"version":"3.49.0"},"reference-count":67,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2002,7,1]],"date-time":"2002-07-01T00:00:00Z","timestamp":1025481600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Trends in Microbiology"],"published-print":{"date-parts":[[2002,7]]},"DOI":"10.1016\/s0966-842x(02)02394-6","type":"journal-article","created":{"date-parts":[[2002,10,11]],"date-time":"2002-10-11T17:19:10Z","timestamp":1034356750000},"page":"318-324","source":"Crossref","is-referenced-by-count":89,"title":["Herpesvirus genetics has come of age"],"prefix":"10.1016","volume":"10","author":[{"given":"Markus","family":"Wagner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zsolt","family":"Ruzsics","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich H.","family":"Koszinowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/S0966-842X(02)02394-6_BIB1","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/978-3-642-66101-3_3","article-title":"Temperature-sensitive mutants of herpesviruses","volume":"70","author":"Schaffer","year":"1975","journal-title":"Curr. Top. Microbiol. Immunol."},{"issue":"Suppl.","key":"10.1016\/S0966-842X(02)02394-6_BIB2","doi-asserted-by":"crossref","first-page":"S42","DOI":"10.1111\/1523-1747.ep12281154","article-title":"Genetics of herpes simplex virus","volume":"83","author":"Schaffer","year":"1984","journal-title":"J. Invest. Dermatol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB3","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/0092-8674(80)90172-5","article-title":"Molecular engineering of the herpes simplex virus genome: insertion of a second L-S junction into the genome causes additional genome inversions","volume":"22","author":"Mocarski","year":"1980","journal-title":"Cell"},{"key":"10.1016\/S0966-842X(02)02394-6_BIB4","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/285333a0","article-title":"Construction in vitro and rescue of a thymidine kinase-deficient deletion mutation of herpes simplex virus","volume":"285","author":"Smiley","year":"1980","journal-title":"Nature"},{"key":"10.1016\/S0966-842X(02)02394-6_BIB5","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/0092-8674(81)90247-6","article-title":"A generalized technique for deletion of specific genes in large genomes: alpha gene 22 of herpes simplex virus 1 is not essential for growth","volume":"25","author":"Post","year":"1981","journal-title":"Cell"},{"key":"10.1016\/S0966-842X(02)02394-6_BIB6","doi-asserted-by":"crossref","first-page":"7213","DOI":"10.1073\/pnas.84.20.7213","article-title":"Insertion and deletion mutagenesis of the human cytomegalovirus genome","volume":"84","author":"Spaete","year":"1987","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB7","first-page":"477","article-title":"Insertional mutagenesis of the murine cytomegalovirus genome: one prominent alpha gene (ie2) is dispensable for growth","volume":"167","author":"Manning","year":"1988","journal-title":"Virology"},{"key":"10.1016\/S0966-842X(02)02394-6_BIB8","doi-asserted-by":"crossref","first-page":"2191","DOI":"10.1128\/JVI.62.6.2191-2195.1988","article-title":"Regeneration of herpesviruses from molecularly cloned subgenomic fragments","volume":"62","author":"van Zijl","year":"1988","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB9","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1006\/viro.1993.1572","article-title":"A cosmid-based system for constructing mutants of herpes simplex virus type 1","volume":"197","author":"Cunningham","year":"1993","journal-title":"Virology"},{"key":"10.1016\/S0966-842X(02)02394-6_BIB10","doi-asserted-by":"crossref","first-page":"7376","DOI":"10.1073\/pnas.90.15.7376","article-title":"Generation of varicella-zoster virus (VZV) and viral mutants from cosmid DNAs: VZV thymidylate synthetase is not essential for replication in vitro","volume":"90","author":"Cohen","year":"1993","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB11","doi-asserted-by":"crossref","first-page":"7298","DOI":"10.1128\/JVI.67.12.7298-7306.1993","article-title":"Epstein\u2013Barr virus recombinants from overlapping cosmid fragments","volume":"67","author":"Tomkinson","year":"1993","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB12","doi-asserted-by":"crossref","first-page":"2044","DOI":"10.1128\/JVI.70.3.2044-2048.1996","article-title":"Defined large-scale alterations of the human cytomegalovirus genome constructed by cotransfection of overlapping cosmids","volume":"70","author":"Kemble","year":"1996","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB13","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/0166-0934(95)01984-7","article-title":"A facile system for construction of HSV-1 variants: site-directed mutation of the UL26 protease gene in HSV-1","volume":"57","author":"Register","year":"1996","journal-title":"J. Virol. Methods"},{"key":"10.1016\/S0966-842X(02)02394-6_BIB14","doi-asserted-by":"crossref","first-page":"5927","DOI":"10.1128\/JVI.72.7.5927-5936.1998","article-title":"Characterization of drug resistance-associated mutations in the human cytomegalovirus DNA polymerase gene by using recombinant mutant viruses generated from overlapping DNA fragments","volume":"72","author":"Cihlar","year":"1998","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB15","doi-asserted-by":"crossref","first-page":"8972","DOI":"10.1128\/JVI.74.19.8972-8979.2000","article-title":"Generation of mutant murine cytomegalovirus strains from overlapping cosmid and plasmid clones","volume":"74","author":"Ehsani","year":"2000","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB16","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1128\/MCB.5.8.1997","article-title":"Activation of immediate-early, early, and late promoters by temperature- sensitive and wild-type forms of herpes simplex virus type 1 protein ICP4","volume":"5","author":"DeLuca","year":"1985","journal-title":"Mol. Cell Biol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB17","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/BF02921558","article-title":"Yeast artificial chromosome cloning","volume":"1","author":"Ramsay","year":"1994","journal-title":"Mol. Biotechnol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB18","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/0958-1669(95)80007-7","article-title":"Techniques in mammalian genome mapping","volume":"6","author":"Schalkwyk","year":"1995","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB19","doi-asserted-by":"crossref","first-page":"8794","DOI":"10.1073\/pnas.89.18.8794","article-title":"Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector","volume":"89","author":"Shizuya","year":"1992","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB20","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1038\/ng0194-84","article-title":"A new bacteriophage P1-derived vector for the propagation of large human DNA fragments","volume":"6","author":"Ioannou","year":"1994","journal-title":"Nat. Genet."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB21","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1093\/nar\/20.5.1083","article-title":"Stable propagation of cosmid sized human DNA inserts in an F factor based vector","volume":"20","author":"Kim","year":"1992","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB22","doi-asserted-by":"crossref","first-page":"4901","DOI":"10.1093\/nar\/26.21.4901","article-title":"Cloning and stable maintenance of DNA fragments over 300 kb in Escherichia coli with conventional plasmid-based vectors","volume":"26","author":"Tao","year":"1998","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB23","doi-asserted-by":"crossref","first-page":"14759","DOI":"10.1073\/pnas.94.26.14759","article-title":"Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome","volume":"94","author":"Messerle","year":"1997","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB24","doi-asserted-by":"crossref","first-page":"7137","DOI":"10.1128\/JVI.72.9.7137-7143.1998","article-title":"An enhanced packaging system for helper-dependent herpes simplex virus vectors","volume":"72","author":"Stavropoulos","year":"1998","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB25","doi-asserted-by":"crossref","first-page":"8245","DOI":"10.1073\/pnas.95.14.8245","article-title":"Propagation and recovery of intact, infectious Epstein\u2013Barr virus from prokaryotic to human cells","volume":"95","author":"Delecluse","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB26","doi-asserted-by":"crossref","first-page":"2787","DOI":"10.1089\/hum.1998.9.18-2787","article-title":"Herpes simplex virus type 1 DNA amplified as bacterial artificial chromosome in Escherichia coli: rescue of replication-competent virus progeny and packaging of amplicon vectors","volume":"9","author":"Saeki","year":"1998","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB27","doi-asserted-by":"crossref","first-page":"8320","DOI":"10.1128\/JVI.73.10.8320-8329.1999","article-title":"Cloning of the human cytomegalovirus (HCMV) genome as an infectious bacterial artificial chromosome in Escherichia coli: a new approach for construction of HCMV mutants","volume":"73","author":"Borst","year":"1999","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB28","doi-asserted-by":"crossref","first-page":"6405","DOI":"10.1128\/JVI.73.8.6405-6414.1999","article-title":"Construction and transposon mutagenesis in Escherichia coli of a full-length infectious clone of pseudorabies virus, an alphaherpesvirus","volume":"73","author":"Smith","year":"1999","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB29","doi-asserted-by":"crossref","first-page":"7056","DOI":"10.1128\/JVI.73.8.7056-7060.1999","article-title":"Systematic excision of vector sequences from the BAC-cloned herpesvirus genome during virus reconstitution","volume":"73","author":"Wagner","year":"1999","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB30","doi-asserted-by":"crossref","first-page":"6964","DOI":"10.1128\/JVI.74.15.6964-6974.2000","article-title":"Cloning and mutagenesis of the murine gammaherpesvirus 68 genome as an infectious bacterial artificial chromosome","volume":"74","author":"Adler","year":"2000","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB31","doi-asserted-by":"crossref","first-page":"11088","DOI":"10.1128\/JVI.74.23.11088-11098.2000","article-title":"Reconstitution of Marek's disease virus serotype 1 (MDV-1) from DNA cloned as a bacterial artificial chromosome and characterization of a glycoprotein B-negative MDV-1 mutant","volume":"74","author":"Schumacher","year":"2000","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB32","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1006\/mgme.2000.3102","article-title":"Molecular cloning of the guinea pig cytomegalovirus (GPCMV) genome as an infectious bacterial artificial chromosome (BAC) in Escherichia coli","volume":"72","author":"McGregor","year":"2001","journal-title":"Mol. Genet. Metab."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB33","doi-asserted-by":"crossref","first-page":"2921","DOI":"10.1128\/JVI.75.6.2921-2928.2001","article-title":"Spontaneous activation of the lytic cycle in cells infected with a recombinant Kaposi's sarcoma-associated virus","volume":"75","author":"Delecluse","year":"2001","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB34","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1128\/JVI.75.4.1870-1878.2001","article-title":"Human cytomegalovirus with IE-2 (UL122) deleted fails to express early lytic genes","volume":"75","author":"Marchini","year":"2001","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB35","doi-asserted-by":"crossref","first-page":"2316","DOI":"10.1128\/jvi.76.5.2316-2328.2002","article-title":"Construction of a self-excisable bacterial artificial chromosome containing the human cytomegalovirus genome and mutagenesis of the diploid TRL\/IRL13 gene","volume":"76","author":"Yu","year":"2002","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB36","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1046\/j.1439-0450.2002.00534.x","article-title":"Cloning of the genomes of equine herpesvirus type 1 (EHV-1) strains KyA and racL11 as bacterial artificial chromosomes (BAC)","volume":"49","author":"Rudolph","year":"2002","journal-title":"J. Vet. Med. B. Infect. Dis. Vet Public Health"},{"key":"10.1016\/S0966-842X(02)02394-6_BIB37","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1038\/sj.gt.3300887","article-title":"Allele replacement: an application that permits rapid manipulation of herpes simplex virus type 1 genomes","volume":"6","author":"Horsburgh","year":"1999","journal-title":"Gene Ther."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB38","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1128\/JVI.69.1.231-238.1995","article-title":"Immortalization of human B lymphocytes by a plasmid containing 71 kilobase pairs of Epstein\u2013Barr virus DNA","volume":"69","author":"Kempkes","year":"1995","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB39","doi-asserted-by":"crossref","first-page":"5978","DOI":"10.1073\/pnas.100127597","article-title":"An efficient recombination system for chromosome engineering in Escherichia coli","volume":"97","author":"Yu","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB40","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1038\/nbt0997-859","article-title":"Homologous recombination based modification in Escherichia coli and germline transmission in transgenic mice of a bacterial artificial chromosome","volume":"15","author":"Yang","year":"1997","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB41","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1038\/7914","article-title":"Rapid identification of essential and nonessential herpesvirus genes by direct transposon mutagenesis see comments","volume":"17","author":"Brune","year":"1999","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB42","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/2417","article-title":"A new logic for DNA engineering using recombination in Escherichia coli","volume":"20","author":"Zhang","year":"1998","journal-title":"Nat. Genet."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB43","doi-asserted-by":"crossref","first-page":"6640","DOI":"10.1073\/pnas.120163297","article-title":"One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products","volume":"97","author":"Datsenko","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB44","doi-asserted-by":"crossref","first-page":"8502","DOI":"10.1128\/JVI.72.11.8502-8509.1998","article-title":"Enhancer requirement for murine cytomegalovirus growth and genetic complementation by the human cytomegalovirus enhancer","volume":"72","author":"Angulo","year":"1998","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB45","doi-asserted-by":"crossref","first-page":"10729","DOI":"10.1128\/JVI.74.22.10729-10736.2000","article-title":"Comparison between human cytomegalovirus pUL97 and murine cytomegalovirus (MCMV) pM97 expressed by MCMV and vaccinia virus: pM97 does not confer ganciclovir sensitivity","volume":"74","author":"Wagner","year":"2000","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB46","doi-asserted-by":"crossref","first-page":"2826","DOI":"10.1128\/JVI.74.6.2826-2839.2000","article-title":"Identification of a boundary domain adjacent to the potent human cytomegalovirus enhancer that represses transcription of the divergent UL127 promoter","volume":"74","author":"Angulo","year":"2000","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB47","doi-asserted-by":"crossref","first-page":"11129","DOI":"10.1128\/JVI.74.23.11129-11136.2000","article-title":"The major immediate-early gene ie3 of mouse cytomegalovirus is essential for viral growth","volume":"74","author":"Angulo","year":"2000","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB48","doi-asserted-by":"crossref","first-page":"7720","DOI":"10.1128\/JVI.74.17.7720-7729.2000","article-title":"Fast screening procedures for random transposon libraries of cloned herpesvirus genomes: mutational analysis of human cytomegalovirus envelope glycoprotein genes","volume":"74","author":"Hobom","year":"2000","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB49","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1084\/jem.194.7.967","article-title":"The multiple immune-evasion genes of murine cytomegalovirus are not redundant: m4 and m152 inhibit antigen presentation in a complementary and cooperative fashion","volume":"194","author":"Kavanagh","year":"2001","journal-title":"J. Exp. Med."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB50","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1128\/JVI.75.3.1450-1458.2001","article-title":"Genetic evidence of an essential role for cytomegalovirus small capsid protein in viral growth","volume":"75","author":"Borst","year":"2001","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB51","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1126\/science.291.5502.303","article-title":"A ribonucleotide reductase homolog of cytomegalovirus and endothelial cell tropism","volume":"291","author":"Brune","year":"2001","journal-title":"Science"},{"key":"10.1016\/S0966-842X(02)02394-6_BIB52","doi-asserted-by":"crossref","first-page":"5692","DOI":"10.1128\/JVI.75.12.5692-5696.2001","article-title":"Virus reconstituted from infectious bacterial artificial chromosome (BAC)-cloned murine gammaherpesvirus 68 acquires wild-type properties in vivo only after excision of BAC vector sequences","volume":"75","author":"Adler","year":"2001","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB53","doi-asserted-by":"crossref","first-page":"2973","DOI":"10.1128\/JVI.76.6.2973-2989.2002","article-title":"Viable human cytomegalovirus recombinant virus with an internal deletion of the IE2 86 gene affects late stages of viral replication","volume":"76","author":"Sanchez","year":"2002","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB54","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1086\/323993","article-title":"Secreted virus-encoded proteins reflect murine cytomegalovirus productivity in organs","volume":"184","author":"Brune","year":"2001","journal-title":"J. Infect. Dis."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB55","doi-asserted-by":"crossref","first-page":"2216","DOI":"10.1074\/jbc.M104178200","article-title":"The glycoprotein gp48 of murine cytomegalovirus. Proteasome-dependent cytosolic dislocation and degradation","volume":"277","author":"Bubeck","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB56","doi-asserted-by":"crossref","first-page":"2316","DOI":"10.1128\/jvi.76.5.2316-2328.2002","article-title":"Construction of a self-excisable bacterial artificial chromosome containing the human cytomegalovirus genome and mutagenesis of the diploid TRL\/IRL13 gene","volume":"76","author":"Yu","year":"2002","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB57","doi-asserted-by":"crossref","first-page":"11307","DOI":"10.1128\/JVI.75.23.11307-11318.2001","article-title":"Glycoproteins E and I of Marek's disease virus serotype 1 are essential for virus growth in cultured cells","volume":"75","author":"Schumacher","year":"2001","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB58","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1099\/0022-1317-83-5-997","article-title":"The products of the UL10 (gM) and the UL49.5 genes of Marek's disease virus serotype 1 are essential for virus growth in cultured cells","volume":"83","author":"Tischer","year":"2002","journal-title":"J. Gen. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB59","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1126\/science.2660262","article-title":"Construction of large DNA segments in Escherichia coli","volume":"244","author":"O'Connor","year":"1989","journal-title":"Science"},{"key":"10.1016\/S0966-842X(02)02394-6_BIB60","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1111\/j.1365-2958.1991.tb00791.x","article-title":"Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon","volume":"5","author":"Blomfield","year":"1991","journal-title":"Mol. Microbiol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB61","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1007\/BF00330901","article-title":"Genetic analysis of sacB, the structural gene of a secreted enzyme, levansucrase of Bacillus subtilis Marburg","volume":"191","author":"Steinmetz","year":"1983","journal-title":"Mol. Gen. Genet."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB62","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/978-1-4615-4199-8_6","article-title":"ET-cloning: think recombination first","volume":"22","author":"Muyrers","year":"2000","journal-title":"Genet. Eng."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB63","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1128\/JVI.76.3.1252-1264.2002","article-title":"Proteolytic processing of human cytomegalovirus glycoprotein B is dispensable for viral growth in culture","volume":"76","author":"Strive","year":"2002","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB64","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1006\/viro.2001.1277","article-title":"Equine herpesvirus type 1 devoid of gM and gp2 is severely impaired in virus egress but not direct cell-to-cell spread","volume":"293","author":"Rudolph","year":"2002","journal-title":"Virology"},{"key":"10.1016\/S0966-842X(02)02394-6_BIB65","doi-asserted-by":"crossref","first-page":"1959","DOI":"10.1128\/JVI.76.4.1959-1970.2002","article-title":"Characterization of Marek's disease virus serotype 1 (MDV-1) deletion mutants that lack UL46 to UL49 genes: MDV-1 UL49, encoding VP22, is indispensable for virus growth","volume":"76","author":"Dorange","year":"2002","journal-title":"J. Virol."},{"key":"10.1016\/S0966-842X(02)02394-6_BIB66","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/0378-1119(95)00193-A","article-title":"Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic\u2013resistance determinant","volume":"158","author":"Cherepanov","year":"1995","journal-title":"Gene"},{"key":"10.1016\/S0966-842X(02)02394-6_BIB67","series-title":"Mobile DNA","first-page":"879","article-title":"Transposable elements and the genetic engineering of bacteria","author":"Berg","year":"1989"}],"container-title":["Trends in Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0966842X02023946?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0966842X02023946?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T14:06:51Z","timestamp":1583417211000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0966842X02023946"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,7]]},"references-count":67,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2002,7]]}},"alternative-id":["S0966842X02023946"],"URL":"https:\/\/doi.org\/10.1016\/s0966-842x(02)02394-6","relation":{},"ISSN":["0966-842X"],"issn-type":[{"value":"0966-842X","type":"print"}],"subject":[],"published":{"date-parts":[[2002,7]]}}}