{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T02:31:29Z","timestamp":1778034689668,"version":"3.51.4"},"reference-count":57,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,7,17]],"date-time":"2013-07-17T00:00:00Z","timestamp":1374019200000},"content-version":"vor","delay-in-days":4886,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunity"],"published-print":{"date-parts":[[2000,3]]},"DOI":"10.1016\/s1074-7613(00)80184-3","type":"journal-article","created":{"date-parts":[[2004,5,1]],"date-time":"2004-05-01T09:50:07Z","timestamp":1083405007000},"page":"313-322","source":"Crossref","is-referenced-by-count":142,"title":["Lck Activity Controls CD4\/CD8 T Cell Lineage Commitment"],"prefix":"10.1016","volume":"12","author":[{"given":"Gabriela","family":"Hern\u00e1ndez-Hoyos","sequence":"first","affiliation":[]},{"given":"Sue J","family":"Sohn","sequence":"additional","affiliation":[]},{"given":"Ellen V","family":"Rothenberg","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9","family":"Alberola-Ila","sequence":"additional","affiliation":[]}],"member":"78","reference":[{"key":"10.1016\/S1074-7613(00)80184-3_BIB1","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1084\/jem.173.6.1421","article-title":"Delayed thymocyte development induced by augmented expression of p56lck","volume":"173","author":"Abraham","year":"1991","journal-title":"J. Exp. Med."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1084\/jem.184.1.9","article-title":"Positive and negative selection invoke distinct signaling pathways","volume":"184","author":"Alberola-Ila","year":"1996","journal-title":"J. Exp. Med."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB3","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1084\/jem.187.8.1249","article-title":"CD3 ligation on immature thymocytes generates antagonist-like signals appropriate for CD8 lineage commitment, independently of T cell receptor specificity","volume":"187","author":"Basson","year":"1998","journal-title":"J. Exp. Med."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB4","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1002\/eji.1830270516","article-title":"Signals through CD8 or CD4 can induce commitment to the CD4 lineage in the thymus","volume":"27","author":"Bommhardt","year":"1997","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB5","doi-asserted-by":"crossref","first-page":"715","DOI":"10.4049\/jimmunol.163.2.715","article-title":"Activation of the extracellular signal-related kinase\/mitogen-activated protein kinase pathway discriminates CD4 versus CD8 lineage commitment in the thymus","volume":"163","author":"Bommhardt","year":"1999","journal-title":"J. Immunol."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB6","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/0092-8674(92)90453-J","article-title":"Exclusion and inclusion of \u03b1 and \u03b2 T cell receptor alleles","volume":"69","author":"Borgulya","year":"1992","journal-title":"Cell"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB7","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1126\/science.8372352","article-title":"Thymic selection of cytotoxic T cells independent of CD8\u03b1-Lck association","volume":"261","author":"Chan","year":"1993","journal-title":"Science"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB8","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/0092-8674(93)90225-F","article-title":"Another view of the selective model of thymocyte selection","volume":"73","author":"Chan","year":"1993","journal-title":"Cell"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB9","doi-asserted-by":"crossref","first-page":"4482","DOI":"10.1002\/j.1460-2075.1994.tb06770.x","article-title":"Role of coreceptors in positive selection and lineage commitment","volume":"13","author":"Chan","year":"1994","journal-title":"EMBO J."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB10","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/1074-7613(94)90078-7","article-title":"Functional commitment to helper T cell lineage precedes positive selection and is independent of T cell receptor MHC specificity","volume":"1","author":"Corbella","year":"1994","journal-title":"Immunity"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB11","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/0092-8674(93)90226-G","article-title":"Evidence for a stochastic mechanism in the differentiation of mature subsets of T lymphocytes","volume":"73","author":"Davis","year":"1993","journal-title":"Cell"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB12","doi-asserted-by":"crossref","first-page":"5146","DOI":"10.1074\/jbc.274.8.5146","article-title":"The Lck SH3 domain is required for activation of the mitogen-activated protein kinase pathway but not the initiation of T-cell antigen receptor signaling","volume":"274","author":"Denny","year":"1999","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB13","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/S1074-7613(00)80446-X","article-title":"Restoration of thymopoiesis in pT\u03b1\u2212\/\u2212 mice by anti-CD3\u03b5 antibody treatment or with transgenes encoding activated Lck or tailless pT\u03b1","volume":"6","author":"Fehling","year":"1997","journal-title":"Immunity"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB14","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/0952-7915(95)80003-4","article-title":"Positive selection of T cells","volume":"7","author":"Fowlkes","year":"1995","journal-title":"Curr. Opin. Immunol."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0022-1759(98)00038-6","article-title":"A novel method for measuring CTL and NK cell-mediated cytotoxicity using annexin V and two-color flow cytometry","volume":"224","author":"Goldberg","year":"1999","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB16","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/S1074-7613(00)80351-9","article-title":"CD8 lineage commitment in the absence of CD8","volume":"6","author":"Goldrath","year":"1997","journal-title":"Immunity"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB17","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1073\/pnas.92.2.354","article-title":"T cells with two functional antigen-specific receptors","volume":"92","author":"Hardardottir","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB18","doi-asserted-by":"crossref","first-page":"3666","DOI":"10.4049\/jimmunol.160.8.3666","article-title":"Identification of a developmentally regulated phase of postselection expansion driven by thymic epithelium","volume":"160","author":"Hare","year":"1998","journal-title":"J. Immunol."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB19","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1084\/jem.184.3.931","article-title":"Requirement for p56lck tyrosine kinase activation in T cell receptor-mediated thymic selection","volume":"184","author":"Hashimoto","year":"1996","journal-title":"J. Exp. Med."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB20","doi-asserted-by":"crossref","first-page":"1807","DOI":"10.1084\/jem.178.5.1807","article-title":"Expression of two \u03b1 chains on the surface of T cells in T cell receptor transgenic mice","volume":"178","author":"Heath","year":"1993","journal-title":"J. Exp. Med."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB21","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/0092-8674(94)90169-4","article-title":"T cell receptor antagonist peptides induce positive selection","volume":"76","author":"Hogquist","year":"1994","journal-title":"Cell"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB22","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1084\/jem.183.3.731","article-title":"The cytoplasmic domain of CD4 promotes the development of CD4 lineage T cells","volume":"183","author":"Itano","year":"1996","journal-title":"J. Exp. Med."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB23","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1126\/science.7509083","article-title":"Sequential interactions of the TCR with two distinct cytoplasmic tyrosine kinases","volume":"263","author":"Iwashima","year":"1994","journal-title":"Science"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB24","doi-asserted-by":"crossref","first-page":"26563","DOI":"10.1074\/jbc.274.37.26563","article-title":"Activation of the protein kinase ERK5\/BMK1 by receptor tyrosine kinases. Identification and characterization of a signaling pathway to the nucleus","volume":"274","author":"Kamakura","year":"1999","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB25","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1038\/341746a0","article-title":"Selective development of CD4+ T cells in transgenic mice expressing a class II MHC-restricted antigen receptor","volume":"341","author":"Kaye","year":"1989","journal-title":"Nature"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB26","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1038\/364729a0","article-title":"Helper T-cell development in the absence of CD4-p56lck association","volume":"364","author":"Killeen","year":"1993","journal-title":"Nature"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB27","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.4049\/jimmunol.140.4.1123","article-title":"Evolution of B cell lineage lymphomas in mice with a retrovirus-induced immunodeficiency syndrome, MAIDS","volume":"140","author":"Klinken","year":"1988","journal-title":"J. Immunol."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB28","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1002\/j.1460-2075.1993.tb05812.x","article-title":"A dominant-negative transgene defines a role for p56lck in thymopoiesis","volume":"12","author":"Levin","year":"1993","journal-title":"EMBO J."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB29","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1084\/jem.181.5.1643","article-title":"Intermediate steps in positive selection","volume":"181","author":"Lundberg","year":"1995","journal-title":"J. Exp. Med."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB30","doi-asserted-by":"crossref","first-page":"5305","DOI":"10.1128\/MCB.10.10.5305","article-title":"Cross-linking of T-cell surface molecules CD4 and CD8 stimulates phosphorylation of the lck tyrosine protein kinase at the autophosphorylation site","volume":"10","author":"Luo","year":"1990","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB31","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/S1074-7613(00)80247-2","article-title":"MHC class II\u2013specific T cells can develop in the CD8 lineage when CD4 is absent","volume":"4","author":"Matechak","year":"1996","journal-title":"Immunity"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB32","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1038\/357161a0","article-title":"Profound block in thymocytes development in mice lacking p56lck","volume":"357","author":"Molina","year":"1992","journal-title":"Nature"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB33","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/1074-7613(94)90077-9","article-title":"An activated lck transgene promotes thymocyte development in RAG-1 mutant mice","volume":"1","author":"Mombaerts","year":"1994","journal-title":"Immunity"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB34","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1146\/annurev.iy.11.040193.002315","article-title":"Regulation of lymphocyte function by protein phosphorylation","volume":"11","author":"Perlmutter","year":"1993","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB35","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.4049\/jimmunol.147.6.1779","article-title":"The majority of CD4+8\u2212 thymocytes are functionally immature","volume":"147","author":"Ramsdell","year":"1991","journal-title":"J. Immunol."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB36","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1146\/annurev.iy.12.040194.003331","article-title":"Selective events in T cell development","volume":"12","author":"Robey","year":"1994","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB37","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/0092-8674(91)90212-H","article-title":"Thymic selection in CD8 transgenic mice supports an instructive model for commitment to a CD4 or CD8 lineage","volume":"64","author":"Robey","year":"1991","journal-title":"Cell"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB38","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1084\/jem.179.6.1997","article-title":"Constitutive CD8 expression allows inefficient maturation of CD4+ helper T cells in class II major histocompatibility complex mutant mice","volume":"179","author":"Robey","year":"1994","journal-title":"J. Exp. Med."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB39","doi-asserted-by":"crossref","first-page":"2497","DOI":"10.4049\/jimmunol.151.5.2497","article-title":"The regulation of the expression of gp39, the CD40 ligand, on normal and cloned CD4+ T cells","volume":"151","author":"Roy","year":"1993","journal-title":"J. Immunol."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB40","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1038\/356718a0","article-title":"Signal for T-cell differentiation to a CD4 cell lineages is delivered by CD4 transmembrane region and\/or cytoplasmic tail","volume":"356","author":"Seong","year":"1992","journal-title":"Nature"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB41","doi-asserted-by":"crossref","first-page":"6598","DOI":"10.4049\/jimmunol.163.12.6598","article-title":"Commitment to the CD4 lineage mediated by extracellular signal-related kinase mitogen-activated protein kinase and lck signaling","volume":"163","author":"Sharp","year":"1999","journal-title":"J. Immunol."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB42","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/S1074-7613(00)80382-9","article-title":"The influence of the MAPK pathway on T cell lineage commitment","volume":"7","author":"Sharp","year":"1997","journal-title":"Immunity"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB43","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/0092-8674(89)90008-1","article-title":"The lck tyrosine protein kinase interacts with the cytoplasmic tail of the CD4 glycoprotein through its unique amino-terminal domain","volume":"59","author":"Shaw","year":"1989","journal-title":"Cell"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB44","doi-asserted-by":"crossref","first-page":"1543","DOI":"10.1084\/jem.176.6.1543","article-title":"Recombinant human CD40 ligand stimulates B cell proliferation and Immunoglobulin E secretion","volume":"176","author":"Spriggs","year":"1992","journal-title":"J. Exp. Med."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB45","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/0092-8674(92)90428-F","article-title":"Genetic evidence for the involvement of the lck tyrosine kinase in signal transduction through the T cell antigen receptor","volume":"70","author":"Straus","year":"1992","journal-title":"Cell"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB46","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/1074-7613(95)90149-3","article-title":"Asymmetric signaling requirements for thymocyte commitment to the CD4+ versus CD8+ T cell lineages","volume":"2","author":"Suzuki","year":"1995","journal-title":"Immunity"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB47","doi-asserted-by":"crossref","first-page":"3252","DOI":"10.1002\/(SICI)1521-4141(199810)28:10<3252::AID-IMMU3252>3.0.CO;2-G","article-title":"Positive selection of CD4+ T cells by TCR-specific antibodies requires low valency TCR cross-linking","volume":"28","author":"Suzuki","year":"1998","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB48","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/0092-8674(90)90090-2","article-title":"Interaction of the unique N-terminal region of tyrosine kinase p56lck with cytoplasmic domains of CD4 and CD8 is mediated by cysteine motifs","volume":"60","author":"Turner","year":"1990","journal-title":"Cell"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB49","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1126\/science.8102208","article-title":"Development of mature CD8+ thymocytes","volume":"261","author":"van Meerwijk","year":"1993","journal-title":"Science"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB50","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1084\/jem.183.3.1053","article-title":"Lck regulates the tyrosine phosphorylation of the T cell receptor subunits and ZAP-70 in murine thymocytes","volume":"183","author":"van Oers","year":"1996","journal-title":"J. Exp. Med."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB51","doi-asserted-by":"crossref","first-page":"1738","DOI":"10.4049\/jimmunol.152.4.1738","article-title":"In vivo cytokine expression in the thymus. CD3high human thymocytes are activated and already functionally differentiated in helper and cytotoxic cells","volume":"152","author":"Vandekerckhove","year":"1994","journal-title":"J. Immunol."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB52","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1084\/jem.170.5.1671","article-title":"Engagement of CD4 and CD8 expressed on immature thymocytes induces activation of intracellular tyrosine phosphorylation pathways","volume":"170","author":"Veillette","year":"1989","journal-title":"J. Exp. Med."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB53","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/0092-8674(88)90053-0","article-title":"The CD4 and CD8 T cell surface antigens are associated with the internal membrane tyrosine-protein kinase p56lck","volume":"55","author":"Veillete","year":"1988","journal-title":"Cell"},{"key":"10.1016\/S1074-7613(00)80184-3_BIB54","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1084\/jem.183.3.713","article-title":"CD4\/CD8 lineage commitment","volume":"183","author":"von Boehmer","year":"1996","journal-title":"J. Exp. Med."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB55","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1093\/intimm\/10.11.1623","article-title":"Precocious expression of T cell functional response genes in vivo in primitive thymocytes before T lineage commitment","volume":"10","author":"Wang","year":"1998","journal-title":"Int. Immunol."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB56","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1084\/jem.178.5.1701","article-title":"Regulation of T cell receptor expression in immature CD4+ CD8+ thymocytes by p56lck tyrosine kinase","volume":"178","author":"Weist","year":"1993","journal-title":"J. Exp. Med."},{"key":"10.1016\/S1074-7613(00)80184-3_BIB57","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.4049\/jimmunol.155.3.1286","article-title":"Functional dissection of the murine lck distal promoter","volume":"155","author":"Wildin","year":"1995","journal-title":"J. Immunol."}],"container-title":["Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1074761300801843?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1074761300801843?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T14:42:23Z","timestamp":1682692943000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1074761300801843"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3]]},"references-count":57,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2000,3]]}},"alternative-id":["S1074761300801843"],"URL":"https:\/\/doi.org\/10.1016\/s1074-7613(00)80184-3","relation":{},"ISSN":["1074-7613"],"issn-type":[{"value":"1074-7613","type":"print"}],"subject":[],"published":{"date-parts":[[2000,3]]}}}