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The system provides a novel three-dimensional gel electrophoresis approach that exploits the property of stacked slice gels. It allows multiple samples simultaneously to react as well as to be separated, offering a two-dimensional (m \u00d7 n) sample loading system. For this purpose, high-throughput multi-micro vessels (MMVs) containing variable numbers of wells (100 wells in this paper) have been used, which are made of 25\u2009mm square-size polyacrylamide gels. Furthermore, after electrophoretic separation, a slice gel containing a desired sample can be easily removed and proceeded to the next step. Different biological reactions as well as successive separation of products were effectively carried out dealing with DNA\/RNA and protein\/peptide. It shows that this system has a diversity of potentials to be developed.<\/jats:p>","DOI":"10.1016\/s1672-0229(06)60013-2","type":"journal-article","created":{"date-parts":[[2006,4,19]],"date-time":"2006-04-19T17:45:22Z","timestamp":1145468722000},"page":"26-33","source":"Crossref","is-referenced-by-count":3,"title":["High-Throughput Three-Dimensional Gel Electrophoresis for Versatile Utilities: A Stacked Slice-Gel System for Separation and Reactions (4SR)"],"prefix":"10.1093","volume":"4","author":[{"given":"Md.","family":"Salimullah","sequence":"first","affiliation":[{"name":"Department of Functional Materials Science, Saitama University , Saitama, 338-8570 , Japan"}]},{"given":"Masaki","family":"Mori","sequence":"additional","affiliation":[{"name":"Department of Functional Materials Science, Saitama University , Saitama, 338-8570 , Japan"}]},{"given":"Koichi","family":"Nishigaki","sequence":"additional","affiliation":[{"name":"Department of Functional Materials Science, Saitama University , Saitama, 338-8570 , Japan"},{"name":"Rational Evolutionary Design of Advanced Biomolecules (REDS) Group, Saitama Small Enterprise Promotion Corporation, SKIP City , Kawaguchi, 333-0844 , Japan"}]}],"member":"286","published-online":{"date-parts":[[2006,4,18]]},"reference":[{"key":"2024051008222427400_bib1","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1038\/sj.ejhg.5201459","article-title":"The amazing complexity of the human transcriptome","volume":"13","author":"Frith","year":"2005","journal-title":"Eur. 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