{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:53:17Z","timestamp":1761540797187,"version":"3.37.3"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T00:00:00Z","timestamp":1715299200000},"content-version":"vor","delay-in-days":6188,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"}],"funder":[{"name":"National Key Basic Research Program of China","award":["2005CB522904"],"award-info":[{"award-number":["2005CB522904"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30470101"],"award-info":[{"award-number":["30470101"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Shigella flexneri is an infectious pathogen that causes dysentery to human, which remains a serious threat to public health, particularly in developing countries. In this study, the global protein expression patterns of S. flexneri during transition from exponential growth to stationary phase in vitro were analyzed by using 2-D PAGE combined with MALDI-TOF MS. In a time-course experiment with five time points, the relative abundance of 49 protein spots varied significantly. Interestingly, a putative outer membrane protein YciD (OmpW) was almost not detected in the exponential growth phase but became one of the most abundant proteins in the whole stationary-phase proteome. Some proteins regulated by the global regulator FNR were also significantly induced (such as AnsB, AspA, FrdAB, and KatG) or repressed (such as AceEF, OmpX, SodA, and SucAB) during the growth phase transition. These proteins may be the key effectors of the bacterial cell cycle or play important roles in the cellular maintenance and stress responses. Our expression profile data provide valuable information for the study of bacterial physiology and form the basis for future proteomic analyses of this pathogen.<\/jats:p>","DOI":"10.1016\/s1672-0229(07)60021-7","type":"journal-article","created":{"date-parts":[[2007,9,23]],"date-time":"2007-09-23T11:12:24Z","timestamp":1190545944000},"page":"111-120","source":"Crossref","is-referenced-by-count":12,"title":["Dynamic Proteome Changes of <i>Shigella Flexneri<\/i> 2a During Transition from Exponential Growth to Stationary Phase"],"prefix":"10.1093","volume":"5","author":[{"given":"Li","family":"Zhu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology , Beijing, 100071 , China"}]},{"given":"Xian-Kai","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology , Beijing, 100071 , China"}]},{"given":"Ge","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology , Beijing, 100071 , China"}]},{"given":"Yi-Dan","family":"Zhi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology , Beijing, 100071 , China"}]},{"given":"Xin","family":"Bu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology , Beijing, 100071 , China"}]},{"given":"Tian-Yi","family":"Ying","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology , Beijing, 100071 , China"}]},{"given":"Er-Ling","family":"Feng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology , Beijing, 100071 , China"}]},{"given":"Jie","family":"Wang","sequence":"additional","affiliation":[{"name":"National Center of Biomedical Analysis , Beijing, 100850 , China"}]},{"given":"Xue-Min","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Center of Biomedical Analysis , Beijing, 100850 , China"}]},{"given":"Pei-Tang","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology , Beijing, 100071 , China"}]},{"given":"Heng-Liang","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology , Beijing, 100071 , China"}]}],"member":"286","published-online":{"date-parts":[[2007,9,22]]},"reference":[{"key":"2024051008231997200_bib1","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1128\/mr.55.2.206-224.1991","article-title":"Genetic basis of virulence in Shigella species","volume":"55","author":"Hale","year":"1991","journal-title":"Microbiol. 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