{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T12:08:31Z","timestamp":1775304511906,"version":"3.50.1"},"reference-count":12,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T00:00:00Z","timestamp":1715299200000},"content-version":"vor","delay-in-days":5184,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A microsatellite-enriched library of plateau pika (Ochotona curzoniae) was constructed according to the strong affinity between biotin and streptavidin. Firstly, genomic DNA was fragmented by ultrasonication, which is a major improvement over traditional methods. Linker-ligated DNA fragments were hybridized with biotinylated microsatellite probes, and then were subjected to streptavidin-coated magnetic beads. PCR amplification was performed to obtain double-stranded DNA fragments containing microsatellites. Ligation and transformation were carried out by using the pGEM-T Vector System I and Escherichia coli DH10B competent cells. Sequencing results showed that 80.2% of clones contained microsatellite repeat motif. Several modifications make this protocol time-efficient and technically easier than the traditional ones; particularly, composition and relative abundance of microsatellite repeats in plateau pika genome were truly represented through the optimized PCR conditions. This method has also been successfully applied to construct microsatellite-enriched genomic libraries of Chinese hamster (Cricetulus griseus) and small abalone [Haliotis diversicolor (Reeve)] with high rates of positive clones, demonstrating its feasibility and stability.<\/jats:p>","DOI":"10.1016\/s1672-0229(10)60007-1","type":"journal-article","created":{"date-parts":[[2010,5,6]],"date-time":"2010-05-06T10:01:09Z","timestamp":1273140069000},"page":"72-76","source":"Crossref","is-referenced-by-count":5,"title":["A Modified Enrichment Method to Construct Microsatellite Library from Plateau Pika Genome (<i>Ochotona Curzoniae<\/i>)"],"prefix":"10.1093","volume":"8","author":[{"given":"Jianing","family":"Geng","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing, 100029 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kexin","family":"Li","sequence":"additional","affiliation":[{"name":"Northwest Plateau Institute of Biology, Chinese Academy of Sciences , Xining, 810001 , China"},{"name":"Graduate University of Chinese Academy of Sciences , Beijing, 100049 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Northwest Plateau Institute of Biology, Chinese Academy of Sciences , Xining, 810001 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songnian","family":"Hu","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing, 100029 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2010,5,5]]},"reference":[{"key":"2024051008265459800_bib1","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1111\/j.1461-0248.2006.00889.x","article-title":"Microsatellites for ecologists: a practical guide to using and evaluating microsatellite markers","volume":"9","author":"Selkoe","year":"2006","journal-title":"Ecol. 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