{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T17:03:44Z","timestamp":1765818224507,"version":"3.48.0"},"reference-count":20,"publisher":"Elsevier","isbn-type":[{"type":"print","value":"9780443347016"}],"license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025]]},"DOI":"10.1016\/bs.mcb.2024.09.004","type":"book-chapter","created":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T08:26:50Z","timestamp":1730795210000},"page":"245-261","source":"Crossref","is-referenced-by-count":0,"title":["Separating the wheat from the chaff: Determination of confidently secreted proteins by including information on their cellular abundance"],"prefix":"10.1016","member":"78","reference":[{"key":"10.1016\/bs.mcb.2024.09.004_bb0010","doi-asserted-by":"crossref","first-page":"2513","DOI":"10.1074\/mcp.M113.031591","article-title":"Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ","volume":"13","author":"Cox","year":"2014","journal-title":"Molecular & Cellular Proteomics"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0015","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1038\/s41592-019-0638-x","article-title":"DIA-NN: Neural networks and interference correction enable deep proteome coverage in high throughput","volume":"17","author":"Demichev","year":"2020","journal-title":"Nature Methods"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0020","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/978-1-4939-0944-5_7","article-title":"Combining pulsed SILAC labeling and click-chemistry for quantitative secretome analysis","volume":"1174","author":"Eichelbaum","year":"2014","journal-title":"Methods in Molecular Biology"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0025","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1038\/nbt.2356","article-title":"Selective enrichment of newly synthesized proteins for quantitative secretome analysis","volume":"30","author":"Eichelbaum","year":"2012","journal-title":"Nature Biotechnology"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0030","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1021\/acs.jproteome.7b00684","article-title":"Mining the Secretome of C2C12 muscle cells: Data dependent experimental approach to analyze protein secretion using label-free quantification and peptide based analysis","volume":"17","author":"Grube","year":"2018","journal-title":"Journal of Proteome Research"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0035","doi-asserted-by":"crossref","first-page":"757","DOI":"10.15252\/msb.20145625","article-title":"Ultrasensitive proteome analysis using paramagnetic bead technology","volume":"10","author":"Hughes","year":"2014","journal-title":"Molecular Systems Biology"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0040","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1038\/s41596-018-0082-x","article-title":"Single-pot, solid-phase-enhanced sample preparation for proteomics experiments","volume":"14","author":"Hughes","year":"2019","journal-title":"Nature Protocols"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0045","doi-asserted-by":"crossref","first-page":"5204","DOI":"10.1038\/s41467-021-25546-y","article-title":"Dynamic tracking and identification of tissue-specific secretory proteins in the circulation of live mice","volume":"12","author":"Kim","year":"2021","journal-title":"Nature Communications"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0050","doi-asserted-by":"crossref","first-page":"3157","DOI":"10.1038\/emboj.2012.173","article-title":"Secretome protein enrichment identifies physiological BACE1 protease substrates in neurons","volume":"31","author":"Kuhn","year":"2012","journal-title":"The EMBO Journal"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0055","doi-asserted-by":"crossref","first-page":"1730","DOI":"10.1021\/acs.jproteome.7b00913","article-title":"Extending the compatibility of the SP3 paramagnetic bead processing approach for proteomics","volume":"17","author":"Moggridge","year":"2018","journal-title":"Journal of Proteome Research"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0060","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2022.878027","article-title":"Secretomics-a key to a comprehensive picture of unconventional protein secretion","volume":"10","author":"Poschmann","year":"2022","journal-title":"Frontiers in Cell and Development Biology"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0065","doi-asserted-by":"crossref","DOI":"10.1002\/pmic.202000178","article-title":"Comparative Secretomics gives access to high confident Secretome data: Evaluation of different methods for the determination of Bona fide secreted proteins","volume":"21","author":"Poschmann","year":"2021","journal-title":"Proteomics"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0070","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbapap.2019.06.004","article-title":"Pitfalls and opportunities in the characterization of unconventionally secreted proteins by secretome analysis","volume":"1867","author":"Schira-Heinen","year":"2019","journal-title":"Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0075","doi-asserted-by":"crossref","DOI":"10.1002\/pmic.201600423","article-title":"An optimised version of the secretome protein enrichment with click sugars (SPECS) method leads to enhanced coverage of the secretome","volume":"17","author":"Serdaroglu","year":"2017","journal-title":"Proteomics"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0080","doi-asserted-by":"crossref","first-page":"7799","DOI":"10.1074\/jbc.REV119.008907","article-title":"Evolution, role in inflammation, and redox control of leaderless secretory proteins","volume":"295","author":"Sitia","year":"2020","journal-title":"The Journal of Biological Chemistry"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0085","doi-asserted-by":"crossref","first-page":"5116","DOI":"10.1073\/pnas.091062498","article-title":"Significance analysis of microarrays applied to the ionizing radiation response","volume":"98","author":"Tusher","year":"2001","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0090","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/978-1-4939-7493-1_7","article-title":"Perseus: A bioinformatics platform for integrative analysis of proteomics data in Cancer research","volume":"1711","author":"Tyanova","year":"2018","journal-title":"Methods in Molecular Biology"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0095","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1038\/nmeth.3901","article-title":"The Perseus computational platform for comprehensive analysis of (prote)omics data","volume":"13","author":"Tyanova","year":"2016","journal-title":"Nature Methods"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0100","article-title":"Cell type-selective secretome profiling in vivo","author":"Wei","year":"2020","journal-title":"Nature Chemical Biology"},{"key":"10.1016\/bs.mcb.2024.09.004_bb0105","doi-asserted-by":"crossref","first-page":"19448","DOI":"10.1038\/s41598-019-55351-z","article-title":"OutCyte: A novel tool for predicting unconventional protein secretion","volume":"9","author":"Zhao","year":"2019","journal-title":"Scientific Reports"}],"container-title":["Methods in Cell Biology","Proteomics in Cell Communication"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0091679X24002097?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0091679X24002097?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T17:00:06Z","timestamp":1765818006000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0091679X24002097"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"ISBN":["9780443347016"],"references-count":20,"URL":"https:\/\/doi.org\/10.1016\/bs.mcb.2024.09.004","relation":{},"ISSN":["0091-679X"],"issn-type":[{"type":"print","value":"0091-679X"}],"subject":[],"published":{"date-parts":[[2025]]}}}