{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T09:37:02Z","timestamp":1780393022268,"version":"3.54.1"},"reference-count":28,"publisher":"Elsevier","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025]]},"DOI":"10.1016\/bs.mcb.2025.02.001","type":"book-chapter","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T20:24:37Z","timestamp":1742243077000},"source":"Crossref","is-referenced-by-count":1,"title":["Guidelines for the assessment of high endothelial venule functionality and health"],"prefix":"10.1016","author":[{"given":"Kathryn A.","family":"Jacobs","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Bergers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/bs.mcb.2025.02.001_bib1","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1038\/nmeth.4463","article-title":"SCENIC: Single-cell regulatory network inference and clustering","volume":"14","author":"Aibar","year":"2017","journal-title":"Nature Methods"},{"key":"10.1016\/bs.mcb.2025.02.001_bib2","first-page":"327","article-title":"Culture characterization of differentiated high endothelial venule cells from human tonsils","volume":"79","author":"Baekkevold","year":"1999","journal-title":"Laboratory Investigation; a Journal of Technical Methods and Pathology"},{"key":"10.1016\/bs.mcb.2025.02.001_bib3","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1016\/j.immuni.2010.10.018","article-title":"Endothelial heparan sulfate controls chemokine presentation in recruitment of lymphocytes and dendritic cells to lymph nodes","volume":"33","author":"Bao","year":"2010","journal-title":"Immunity"},{"key":"10.1016\/bs.mcb.2025.02.001_bib4","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1126\/science.aal3662","article-title":"Lymph node blood vessels provide exit routes for metastatic tumor cell dissemination in mice","volume":"359","author":"Brown","year":"2018","journal-title":"Science (New York, N. Y.)"},{"key":"10.1016\/bs.mcb.2025.02.001_bib5","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.immuni.2005.10.002","article-title":"Lymphotoxin-beta receptor signaling is required for the homeostatic control of HEV differentiation and function","volume":"23","author":"Browning","year":"2005","journal-title":"Immunity"},{"key":"10.1016\/bs.mcb.2025.02.001_bib6","doi-asserted-by":"crossref","first-page":"3798","DOI":"10.1038\/s41467-020-17291-5","article-title":"A molecular map of murine lymph node blood vascular endothelium at single cell resolution","volume":"11","author":"Brulois","year":"2020","journal-title":"Nature Communications"},{"key":"10.1016\/bs.mcb.2025.02.001_bib7","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/S1074-7613(00)00074-1","article-title":"Chemokines trigger immediate beta2 integrin affinity and mobility changes: Differential regulation and roles in lymphocyte arrest under flow","volume":"13","author":"Constantin","year":"2000","journal-title":"Immunity"},{"key":"10.1016\/bs.mcb.2025.02.001_bib8","doi-asserted-by":"crossref","first-page":"2043","DOI":"10.1016\/S0002-9440(10)65523-X","article-title":"Heterogeneity of endothelial cells: The specialized phenotype of human high endothelial venules characterized by suppression subtractive hybridization","volume":"155","author":"Girard","year":"1999","journal-title":"The American Journal of Pathology"},{"key":"10.1016\/bs.mcb.2025.02.001_bib9","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/0167-5699(95)80023-9","article-title":"High endothelial venules (HEVs): Specialized endothelium for lymphocyte migration","volume":"16","author":"Girard","year":"1995","journal-title":"Immunology Today"},{"key":"10.1016\/bs.mcb.2025.02.001_bib10","doi-asserted-by":"crossref","first-page":"495","DOI":"10.4049\/jimmunol.157.2.495","article-title":"Sophisticated strategies for information encounter in the lymph node: The reticular network as a conduit of soluble information and a highway for cell traffic","volume":"157","author":"Gretz","year":"1996","journal-title":"The Journal of Immunology : Official Journal of the American Association of Immunologists"},{"key":"10.1016\/bs.mcb.2025.02.001_bib11","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S1074-7613(01)00166-2","article-title":"The alpha(1,3)fucosyltransferases FucT-IV and FucT-VII exert collaborative control over selectin-dependent leukocyte recruitment and lymphocyte homing","volume":"15","author":"Homeister","year":"2001","journal-title":"Immunity"},{"key":"10.1016\/bs.mcb.2025.02.001_bib12","doi-asserted-by":"crossref","first-page":"1600","DOI":"10.1016\/j.ccell.2022.11.002","article-title":"Cancer immunotherapies transition endothelial cells into HEVs that generate TCF1+ T lymphocyte niches through a feed-forward loop","volume":"40","author":"Hua","year":"2022","journal-title":"Cancer Cell"},{"key":"10.1016\/bs.mcb.2025.02.001_bib13","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/BF00300690","article-title":"Expression of the intercellular adhesion molecule-1 on high endothelial venules and on non-lymphoid antigen handling cells: Interdigitating cells, antigen transporting cells and follicular dendritic cells","volume":"279","author":"Maeda","year":"1995","journal-title":"Cell and Tissue Research"},{"issue":"Pt 1","key":"10.1016\/bs.mcb.2025.02.001_bib14","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1242\/jcs.106.1.109","article-title":"VCAM-1 is a CS1 peptide-inhibitable adhesion molecule expressed by lymph node high endothelium","volume":"106","author":"May","year":"1993","journal-title":"Journal of Cell Science"},{"key":"10.1016\/bs.mcb.2025.02.001_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2022.101267","article-title":"Analyses of murine lymph node endothelial cell subsets using single-cell RNA sequencing and spectral flow cytometry","volume":"3","author":"Menzel","year":"2022","journal-title":"STAR Protocols"},{"key":"10.1016\/bs.mcb.2025.02.001_bib16","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.trecan.2020.10.001","article-title":"The dual role of high endothelial venules in cancer progression versus immunity","volume":"7","author":"Milutinovic","year":"2021","journal-title":"Trends Cancer"},{"key":"10.1016\/bs.mcb.2025.02.001_bib17","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1084\/jem.20121462","article-title":"Endothelial cell-specific lymphotoxin-\u03b2 receptor signaling is critical for lymph node and high endothelial venule formation","volume":"210","author":"Onder","year":"2013","journal-title":"The Journal of Experimental Medicine"},{"key":"10.1016\/bs.mcb.2025.02.001_bib18","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1126\/science.aal3622","article-title":"Lymph node metastases can invade local blood vessels, exit the node, and colonize distant organs in mice","volume":"359","author":"Pereira","year":"2018","journal-title":"Science (New York, N. Y.)"},{"key":"10.1016\/bs.mcb.2025.02.001_bib19","doi-asserted-by":"crossref","first-page":"10365","DOI":"10.1158\/0008-5472.CAN-06-2977","article-title":"Preparing the \u2018soil\u2019: The primary tumor induces vasculature reorganization in the sentinel lymph node before the arrival of metastatic cancer cells","volume":"66","author":"Qian","year":"2006","journal-title":"Cancer Research"},{"key":"10.1016\/bs.mcb.2025.02.001_bib20","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1093\/intimm\/dxn110","article-title":"The conduit system of the lymph node","volume":"20","author":"Roozendaal","year":"2008","journal-title":"International Immunology"},{"key":"10.1016\/bs.mcb.2025.02.001_bib21","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1146\/annurev.immunol.21.090501.080131","article-title":"Ligands for L-selectin: Homing, inflammation, and beyond","volume":"22","author":"Rosen","year":"2004","journal-title":"Annual Review of Immunology"},{"key":"10.1016\/bs.mcb.2025.02.001_bib22","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1083\/jcb.107.5.1853","article-title":"Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes","volume":"107","author":"Streeter","year":"1988","journal-title":"The Journal of Cell Biology"},{"key":"10.1016\/bs.mcb.2025.02.001_bib23","doi-asserted-by":"crossref","first-page":"448","DOI":"10.4049\/jimmunol.1203061","article-title":"Role of high endothelial venule-expressed heparan sulfate in chemokine presentation and lymphocyte homing","volume":"191","author":"Tsuboi","year":"2013","journal-title":"The Journal of Immunology : Official Journal of the American Association of Immunologists"},{"key":"10.1016\/bs.mcb.2025.02.001_bib24","doi-asserted-by":"crossref","first-page":"3116","DOI":"10.1016\/j.celrep.2019.02.042","article-title":"Single-cell analysis reveals heterogeneity of high endothelial venules and different regulation of genes controlling lymphocyte entry to lymph nodes","volume":"26","author":"Veerman","year":"2019","journal-title":"Cell Reports"},{"key":"10.1016\/bs.mcb.2025.02.001_bib25","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.736670","article-title":"High endothelial venules: A vascular perspective on tertiary lymphoid structures in cancer","volume":"12","author":"Vella","year":"2021","journal-title":"Frontiers in Immunology"},{"key":"10.1016\/bs.mcb.2025.02.001_bib26","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.ccell.2023.02.002","article-title":"High endothelial venules in cancer: Regulation, function, and therapeutic implication","volume":"41","author":"Vella","year":"2023","journal-title":"Cancer Cell"},{"key":"10.1016\/bs.mcb.2025.02.001_bib27","doi-asserted-by":"crossref","first-page":"301","DOI":"10.3389\/fimmu.2016.00301","article-title":"Biosynthesis and functional significance of peripheral node addressin in cancer-associated TLO","volume":"7","author":"Weinstein","year":"2016","journal-title":"Frontiers in Immunology"},{"key":"10.1016\/bs.mcb.2025.02.001_bib28","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1016\/S0092-8674(01)00394-4","article-title":"Novel sulfated lymphocyte homing receptors and their control by a Core1 extension beta 1,3-N-acetylglucosaminyltransferase","volume":"105","author":"Yeh","year":"2001","journal-title":"Cell"}],"container-title":["Methods in Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0091679X25000548?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0091679X25000548?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T06:58:04Z","timestamp":1750402684000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0091679X25000548"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":28,"URL":"https:\/\/doi.org\/10.1016\/bs.mcb.2025.02.001","relation":{},"ISSN":["0091-679X"],"issn-type":[{"value":"0091-679X","type":"print"}],"subject":[],"published":{"date-parts":[[2025]]}}}