{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T02:49:10Z","timestamp":1775789350418,"version":"3.50.1"},"reference-count":27,"publisher":"Elsevier","isbn-type":[{"value":"9780443428654","type":"print"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82400200"],"award-info":[{"award-number":["82400200"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005145","name":"Basic Research Program of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20243007"],"award-info":[{"award-number":["BK20243007"]}],"id":[{"id":"10.13039\/501100005145","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005952","name":"Ministry of Human Resources and Social Security","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005952","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002338","name":"Ministry of Education of the People's Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002338","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007824","name":"Soochow University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007824","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1016\/bs.mcb.2026.01.006","type":"book-chapter","created":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T12:14:03Z","timestamp":1769688843000},"page":"199-216","source":"Crossref","is-referenced-by-count":0,"title":["CRISPR-Cas9 knockout screens to identify drug resistance genes in acute myeloid leukemia"],"prefix":"10.1016","author":[{"given":"Xin","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaqi","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiayi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanyu","family":"Meng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yufang","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"10","key":"10.1016\/bs.mcb.2026.01.006_bib1","doi-asserted-by":"crossref","first-page":"1482","DOI":"10.1080\/10428194.2024.2360536","article-title":"A temporal and multinational assessment of acute myeloid leukemia (AML) cancer incidence, survival, and disease burden","volume":"65","author":"Anderson","year":"2024","journal-title":"Leukemia & Lymphoma"},{"issue":"1","key":"10.1016\/bs.mcb.2026.01.006_bib2","doi-asserted-by":"crossref","DOI":"10.1038\/s43586-021-00093-4","article-title":"High-content CRISPR screening","volume":"2","author":"Bock","year":"2022","journal-title":"Nature Reviews Methods Primers"},{"issue":"4","key":"10.1016\/bs.mcb.2026.01.006_bib3","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1038\/s41375-023-01833-z","article-title":"KDM6 demethylases integrate DNA repair gene regulation and loss of KDM6A sensitizes human acute myeloid leukemia to PARP and BCL2 inhibition","volume":"37","author":"Boila","year":"2023","journal-title":"Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U. K"},{"issue":"11","key":"10.1016\/bs.mcb.2026.01.006_bib4","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1038\/s43018-021-00264-y","article-title":"Mitochondrial inhibitors circumvent adaptive resistance to venetoclax and cytarabine combination therapy in acute myeloid leukemia","volume":"2","author":"Bosc","year":"2021","journal-title":"Nature Cancer"},{"issue":"20","key":"10.1016\/bs.mcb.2026.01.006_bib5","doi-asserted-by":"crossref","first-page":"3040","DOI":"10.1182\/blood.2021011510","article-title":"Critical role of Lama4 for hematopoiesis regeneration and acute myeloid leukemia progression","volume":"139","author":"Cai","year":"2022","journal-title":"Blood"},{"issue":"2","key":"10.1016\/bs.mcb.2026.01.006_bib6","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/nrg.2017.97","article-title":"Am I ready for CRISPR? A user\u2019s guide to genetic screens","volume":"19","author":"Doench","year":"2018","journal-title":"Nature Reviews. Genetics"},{"issue":"12","key":"10.1016\/bs.mcb.2026.01.006_bib7","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1182\/blood.2022016867","article-title":"Diagnosis and management of AML in adults: 2022 recommendations from an international expert panel on behalf of the ELN","volume":"140","author":"Dohner","year":"2022","journal-title":"Blood"},{"issue":"2","key":"10.1016\/bs.mcb.2026.01.006_bib8","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1038\/nm.4265","article-title":"Targeting SAMHD1 with the Vpx protein to improve cytarabine therapy for hematological malignancies","volume":"23","author":"Herold","year":"2017","journal-title":"Nature Medicine"},{"issue":"16","key":"10.1016\/bs.mcb.2026.01.006_bib9","doi-asserted-by":"crossref","first-page":"4402","DOI":"10.1158\/0008-5472.CAN-16-1627","article-title":"A genome-wide CRISPR screen identifies genes critical for resistance to FLT3 inhibitor AC220","volume":"77","author":"Hou","year":"2017","journal-title":"Cancer Research"},{"issue":"2","key":"10.1016\/bs.mcb.2026.01.006_bib10","doi-asserted-by":"crossref","first-page":"308","DOI":"10.3324\/haematol.2022.280801","article-title":"The clinical impact of the molecular landscape of acute myeloid leukemia","volume":"108","author":"Kayser","year":"2023","journal-title":"Haematologica"},{"issue":"10","key":"10.1016\/bs.mcb.2026.01.006_bib11","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1158\/2159-8290.CD-16-0313","article-title":"Efficacy and biological correlates of response in a phase II study of venetoclax monotherapy in patients with acute myelogenous leukemia","volume":"6","author":"Konopleva","year":"2016","journal-title":"Cancer Discov"},{"issue":"7","key":"10.1016\/bs.mcb.2026.01.006_bib12","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1038\/nm.4125","article-title":"Distinct evolution and dynamics of epigenetic and genetic heterogeneity in acute myeloid leukemia","volume":"22","author":"Li","year":"2016","journal-title":"Nature Medicine"},{"issue":"12","key":"10.1016\/bs.mcb.2026.01.006_bib13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13059-014-0554-4","article-title":"MAGeCK enables robust identification of essential genes from genome-scale CRISPR\/Cas9 knockout screens","volume":"15","author":"Li","year":"2014","journal-title":"Genome Biology"},{"issue":"2","key":"10.1016\/bs.mcb.2026.01.006_bib14","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1158\/2159-8290.CD-20-1851","article-title":"An in vivo CRISPR screening platform for prioritizing therapeutic targets in AML","volume":"12","author":"Lin","year":"2022","journal-title":"Cancer Discovery"},{"issue":"8","key":"10.1016\/bs.mcb.2026.01.006_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.heliyon.2024.e29382","article-title":"CRISPR screens in mechanism and target discovery for AML","volume":"10","author":"Lin","year":"2024","journal-title":"Heliyon"},{"issue":"5","key":"10.1016\/bs.mcb.2026.01.006_bib16","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1038\/s41568-020-0245-2","article-title":"Bone marrow niches in haematological malignancies","volume":"20","author":"Mendez-Ferrer","year":"2020","journal-title":"Nature Reviews. Cancer"},{"issue":"5","key":"10.1016\/bs.mcb.2026.01.006_bib17","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1016\/j.cell.2024.01.042","article-title":"Past, present, and future of CRISPR genome editing technologies","volume":"187","author":"Pacesa","year":"2024","journal-title":"Cell"},{"issue":"10","key":"10.1016\/bs.mcb.2026.01.006_bib18","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1038\/s41576-025-00873-8","article-title":"Methods and applications of in vivo CRISPR screening","volume":"26","author":"Santinha","year":"2025","journal-title":"Nature Reviews. Genetics"},{"issue":"5","key":"10.1016\/bs.mcb.2026.01.006_bib19","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1002\/ajh.27625","article-title":"Acute myeloid leukemia: 2025 Update on diagnosis, risk-stratification, and management","volume":"100","author":"Shimony","year":"2025","journal-title":"American Journal of Hematology"},{"issue":"6","key":"10.1016\/bs.mcb.2026.01.006_bib20","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1182\/blood-2017-04-779405","article-title":"Enasidenib in mutant IDH2 relapsed or refractory acute myeloid leukemia","volume":"130","author":"Stein","year":"2017","journal-title":"Blood"},{"issue":"1","key":"10.1016\/bs.mcb.2026.01.006_bib21","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/s41375-021-01350-x","article-title":"Catch me if you can: How AML and its niche escape immunotherapy","volume":"36","author":"Tettamanti","year":"2022","journal-title":"Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U. K"},{"issue":"7755","key":"10.1016\/bs.mcb.2026.01.006_bib22","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1038\/s41586-019-1104-8","article-title":"The bone marrow microenvironment at single-cell resolution","volume":"569","author":"Tikhonova","year":"2019","journal-title":"Nature"},{"issue":"2","key":"10.1016\/bs.mcb.2026.01.006_bib23","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1002\/ijc.2910260208","article-title":"Establishment and characterization of a human acute monocytic leukemia cell line (THP-1)","volume":"26","author":"Tsuchiya","year":"1980","journal-title":"International Journal of Cancer"},{"issue":"12","key":"10.1016\/bs.mcb.2026.01.006_bib24","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1002\/gcc.22806","article-title":"Clonal evolution of acute myeloid leukemia from diagnosis to relapse","volume":"58","author":"Vosberg","year":"2019","journal-title":"Genes, Chromosomes & Cancer"},{"issue":"1","key":"10.1016\/bs.mcb.2026.01.006_bib25","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/s12943-022-01518-8","article-title":"Current applications and future perspective of CRISPR\/Cas9 gene editing in cancer","volume":"21","author":"Wang","year":"2022","journal-title":"Molecular Cancer"},{"issue":"4","key":"10.1016\/bs.mcb.2026.01.006_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2023.102762","article-title":"Protocol of CRISPR-Cas9 knockout screens for identifying ferroptosis regulators","volume":"4","author":"Yang","year":"2023","journal-title":"STAR Protocols"},{"issue":"1","key":"10.1016\/bs.mcb.2026.01.006_bib27","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1038\/s41392-021-00870-3","article-title":"Activation of RAS\/MAPK pathway confers MCL-1 mediated acquired resistance to BCL-2 inhibitor venetoclax in acute myeloid leukemia","volume":"7","author":"Zhang","year":"2022","journal-title":"Signal Transduction Targeted Therapy"}],"container-title":["Methods in Cell Biology","Cell Death - Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0091679X26000063?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0091679X26000063?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T02:04:17Z","timestamp":1775786657000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0091679X26000063"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9780443428654"],"references-count":27,"URL":"https:\/\/doi.org\/10.1016\/bs.mcb.2026.01.006","relation":{},"ISSN":["0091-679X"],"issn-type":[{"value":"0091-679X","type":"print"}],"subject":[],"published":{"date-parts":[[2026]]}}}