{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T21:06:12Z","timestamp":1779483972705,"version":"3.53.1"},"reference-count":25,"publisher":"Elsevier","isbn-type":[{"value":"9780443431203","type":"print"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100010434","name":"'la Caixa' Foundation","doi-asserted-by":"publisher","award":["LCF\/PR\/HR23\/52430018"],"award-info":[{"award-number":["LCF\/PR\/HR23\/52430018"]}],"id":[{"id":"10.13039\/100010434","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000289","name":"Cancer Research UK","doi-asserted-by":"publisher","award":["C355\/A26819"],"award-info":[{"award-number":["C355\/A26819"]}],"id":[{"id":"10.13039\/501100000289","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PID2021\\u2013128283OA-I00"],"award-info":[{"award-number":["PID2021\\u2013128283OA-I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["0011\\u20131411-2022\\u2013000088"],"award-info":[{"award-number":["0011\\u20131411-2022\\u2013000088"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PLEC2021\\u2013008094 MCIN\/AEI\/10.13039\/ 501100011033"],"award-info":[{"award-number":["PLEC2021\\u2013008094 MCIN\/AEI\/10.13039\/ 501100011033"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PID2022\\u2013137265OB-I0"],"award-info":[{"award-number":["PID2022\\u2013137265OB-I0"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PID2022\\u2013141895OB-I00"],"award-info":[{"award-number":["PID2022\\u2013141895OB-I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001271","name":"Chartered Institute of Management Accountants","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001271","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018866","name":"Paula and Rodger Riney Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018866","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1016\/bs.mcb.2026.01.018","type":"book-chapter","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T04:53:50Z","timestamp":1770267230000},"page":"53-74","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of murine CAR-T cell\u2013mediated cytotoxicity in solid tumor models"],"prefix":"10.1016","author":[{"given":"Celia","family":"Mart\u00edn-Otal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1lvaro","family":"G\u00f3mez-Mor\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noa","family":"Beatriz Mart\u00edn-Cofreces","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Lozano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/bs.mcb.2026.01.018_bib1","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1038\/s41571-023-00832-4","article-title":"CAR T cell therapy for patients with solid tumours: Key lessons to learn and unlearn","volume":"21","author":"Albelda","year":"2024","journal-title":"Nature Reviews Clinical Oncology"},{"key":"10.1016\/bs.mcb.2026.01.018_bib2","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/bs.ircmb.2022.03.004","article-title":"Enabling CAR-T cells for solid tumors: Rage against the suppressive tumor microenvironment","volume":"370","author":"Anto\u00f1ana-Vildosola","year":"2022","journal-title":"International Review of Cell and Molecular Biology"},{"key":"10.1016\/bs.mcb.2026.01.018_bib3","article-title":"Potency assays and biomarkers for cell-based advanced therapy medicinal products","volume":"14","author":"Capelli","year":"2023"},{"issue":"5","key":"10.1016\/bs.mcb.2026.01.018_bib4","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1038\/s41591-018-0010-1","article-title":"Determinants of response and resistance to CD19 chimeric antigen receptor (CAR) T cell therapy of chronic lymphocytic leukemia","volume":"24","author":"Fraietta","year":"2018","journal-title":"Nature Medicine"},{"issue":"9","key":"10.1016\/bs.mcb.2026.01.018_bib5","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1158\/2326-6066.CIR-17-0405","article-title":"Reducing ex vivo culture improves the antileukemic activity of chimeric antigen receptor (CAR) T cells","volume":"6","author":"Ghassemi","year":"2018","journal-title":"Cancer Immunology Research"},{"key":"10.1016\/bs.mcb.2026.01.018_bib6","article-title":"Fine-tuning the antigen sensitivity of CAR T cells: emerging strategies and current challenges","volume":"14","author":"Harrer","year":"2023"},{"issue":"2","key":"10.1016\/bs.mcb.2026.01.018_bib7","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.immuni.2016.01.021","article-title":"Distinct signaling of coreceptors regulates specific metabolism pathways and impacts memory development in CAR T cells","volume":"44","author":"Kawalekar","year":"2016","journal-title":"Immunity"},{"key":"10.1016\/bs.mcb.2026.01.018_bib8","doi-asserted-by":"crossref","DOI":"10.1038\/s41596-020-00467-0","article-title":"Comparative analysis of assays to measure CAR T-cell-mediated cytotoxicity","author":"Kiesgen","year":"2021"},{"key":"10.1016\/bs.mcb.2026.01.018_bib9","doi-asserted-by":"crossref","unstructured":"Krawczyk, M., Fernandez-Fuentes, N., Fidyt, K., Winiarski, T., Pepek, M., Graczyk-Jarzynka, A., ... Winiarska, M. (2025). The costimulatory domain influences CD19 CAR-T cell resistance development in B-cell malignancies. https:\/\/doi.org\/10.1101\/2025.02.28.640707.","DOI":"10.1101\/2025.02.28.640707"},{"issue":"9","key":"10.1016\/bs.mcb.2026.01.018_bib10","doi-asserted-by":"crossref","first-page":"2295","DOI":"10.1038\/s41591-023-02491-5","article-title":"Mechanisms of antigen escape from BCMA- or GPRC5D-targeted immunotherapies in multiple myeloma","volume":"29","author":"Lee","year":"2023","journal-title":"Nature Medicine"},{"key":"10.1016\/bs.mcb.2026.01.018_bib11","first-page":"81","article-title":"Evaluation of CAR-T cell cytotoxicity: Real-time impedance-based analysis","volume":"167","author":"Lisby","year":"2022"},{"issue":"6","key":"10.1016\/bs.mcb.2026.01.018_bib12","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nm.3838","article-title":"4-1BB costimulation ameliorates T cell exhaustion induced by tonic signaling of chimeric antigen receptors","volume":"21","author":"Long","year":"2015","journal-title":"Nature Medicine"},{"issue":"7786","key":"10.1016\/bs.mcb.2026.01.018_bib13","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/s41586-019-1805-z","article-title":"c-Jun overexpression in CAR T cells induces exhaustion resistance","volume":"576","author":"Lynn","year":"2019","journal-title":"Nature"},{"issue":"8","key":"10.1016\/bs.mcb.2026.01.018_bib14","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1182\/blood-2015-02-629527","article-title":"A T-cell-directed chimeric antigen receptor for the selective treatment of T-cell malignancies","volume":"126","author":"Mamonkin","year":"2015","journal-title":"Blood"},{"issue":"8","key":"10.1016\/bs.mcb.2026.01.018_bib15","doi-asserted-by":"crossref","DOI":"10.1136\/jitc-2021-004479","article-title":"Targeting the extra domain A of fibronectin for cancer therapy with CAR-T cells","volume":"10","author":"Mart\u00edn-Otal","year":"2022","journal-title":"Journal for ImmunoTherapy of Cancer"},{"issue":"2","key":"10.1016\/bs.mcb.2026.01.018_bib16","doi-asserted-by":"crossref","DOI":"10.1136\/jitc-2024-009468","article-title":"Phosphatidylserine as a tumor target for CAR-T cell therapy","volume":"13","author":"Mart\u00edn-Otal","year":"2025","journal-title":"Journal for ImmunoTherapy of Cancer"},{"issue":"5","key":"10.1016\/bs.mcb.2026.01.018_bib17","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1056\/NEJMoa1709866","article-title":"Tisagenlecleucel in Children and Young Adults with B-Cell Lymphoblastic Leukemia","volume":"378","author":"Maude","year":"2018","journal-title":"New England Journal of Medicine"},{"key":"10.1016\/bs.mcb.2026.01.018_bib18","doi-asserted-by":"crossref","DOI":"10.1038\/s41577-021-00547-6","article-title":"Cytokine release syndrome and associated neurotoxicity in cancer immunotherapy","author":"Morris","year":"2022"},{"issue":"26","key":"10.1016\/bs.mcb.2026.01.018_bib19","doi-asserted-by":"crossref","first-page":"2531","DOI":"10.1056\/NEJMoa1707447","article-title":"Axicabtagene ciloleucel CAR T-cell therapy in refractory large B-cell lymphoma","volume":"377","author":"Neelapu","year":"2017","journal-title":"New England Journal of Medicine"},{"key":"10.1016\/bs.mcb.2026.01.018_bib20","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.jim.2014.01.012","article-title":"An impedance-based cytotoxicity assay for real-time and label-free assessment of T-cell-mediated killing of adherent cells","volume":"405","author":"Peper","year":"2014","journal-title":"Journal of Immunological Methods"},{"key":"10.1016\/bs.mcb.2026.01.018_bib21","doi-asserted-by":"crossref","DOI":"10.1038\/s41571-019-0297-y","article-title":"Engineering strategies to overcome the current roadblocks in CAR T cell therapy","author":"Rafiq","year":"2020"},{"key":"10.1016\/bs.mcb.2026.01.018_bib22","doi-asserted-by":"crossref","DOI":"10.1038\/nature22395","article-title":"Therapeutic T cell engineering","author":"Sadelain","year":"2017"},{"key":"10.1016\/bs.mcb.2026.01.018_bib23","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23063154","article-title":"Preclinical evaluation of CAR T cell function: In vitro and in vivo models","author":"Si","year":"2022"},{"key":"10.1016\/bs.mcb.2026.01.018_bib24","doi-asserted-by":"crossref","DOI":"10.1038\/s41408-021-00459-7","article-title":"CAR-T cell therapy: Current limitations and potential strategies","author":"Sterner","year":"2021"},{"issue":"1","key":"10.1016\/bs.mcb.2026.01.018_bib25","article-title":"Inhibition of VEGF signaling prevents exhaustion and enhances anti-leukemia efficacy of CAR-T cells via Wnt\/\u03b2-catenin pathway","volume":"23","author":"Wu","year":"2025","journal-title":"Journal of Translational Medicine"}],"container-title":["Methods in Cell Biology","Advanced Methodologies to Study and Enhance Cellular Cytotoxicity Against Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0091679X2600018X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0091679X2600018X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T20:26:34Z","timestamp":1779481594000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0091679X2600018X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9780443431203"],"references-count":25,"URL":"https:\/\/doi.org\/10.1016\/bs.mcb.2026.01.018","relation":{},"ISSN":["0091-679X"],"issn-type":[{"value":"0091-679X","type":"print"}],"subject":[],"published":{"date-parts":[[2026]]}}}