{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T04:53:13Z","timestamp":1785127993254,"version":"3.55.0"},"reference-count":59,"publisher":"Elsevier","isbn-type":[{"value":"9780128021927","type":"print"}],"license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015]]},"DOI":"10.1016\/bs.mie.2015.03.012","type":"book-chapter","created":{"date-parts":[[2015,7,23]],"date-time":"2015-07-23T15:03:50Z","timestamp":1437663830000},"page":"161-185","source":"Crossref","is-referenced-by-count":38,"title":["High-Resolution Mapping of N6-Methyladenosine in Transcriptome and Genome Using a Photo-Crosslinking-Assisted Strategy"],"prefix":"10.1016","author":[{"given":"Kai","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guan-Zheng","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/bs.mie.2015.03.012_bb0005","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.stem.2014.09.019","article-title":"m6A RNA modification controls cell fate transition in mammalian embryonic stem cells","volume":"15","author":"Batista","year":"2014","journal-title":"Cell Stem Cell"},{"key":"10.1016\/bs.mie.2015.03.012_bb0010","series-title":"Fine-tuning of RNA functions by modification and editing","first-page":"141","article-title":"The biosynthesis and functional roles of methylated nucleosides in eukaryotic mRNA","author":"Bokar","year":"2005"},{"key":"10.1016\/bs.mie.2015.03.012_bb0015","doi-asserted-by":"crossref","first-page":"17697","DOI":"10.1016\/S0021-9258(17)32497-3","article-title":"Characterization and partial purification of mRNA N6-adenosine methyltransferase from HeLa cell nuclei. Internal mRNA methylation requires a multisubunit complex","volume":"269","author":"Bokar","year":"1994","journal-title":"Journal of Biological Chemistry"},{"key":"10.1016\/bs.mie.2015.03.012_bb0020","first-page":"1233","article-title":"Purification and cDNA cloning of the AdoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase","volume":"3","author":"Bokar","year":"1997","journal-title":"RNA"},{"key":"10.1016\/bs.mie.2015.03.012_bb0025","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1016\/0092-8674(90)90271-F","article-title":"E. coli oriC and the dnaA gene promoter are sequestered from dam methyltransferase following the passage of the chromosomal replication fork","volume":"62","author":"Campbell","year":"1990","journal-title":"Cell"},{"key":"10.1016\/bs.mie.2015.03.012_bb0030","doi-asserted-by":"crossref","first-page":"2879","DOI":"10.1093\/nar\/6.8.2879","article-title":"Identification and mapping of N6-methyladenosine containing sequences in Simian Virus 40 RNA","volume":"6","author":"Canaani","year":"1979","journal-title":"Nucleic Acids Research"},{"key":"10.1016\/bs.mie.2015.03.012_bb0035","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/0092-8674(88)90479-5","article-title":"DNA methylation and gene activity","volume":"53","author":"Cedar","year":"1988","journal-title":"Cell"},{"key":"10.1016\/bs.mie.2015.03.012_bb0040","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.stem.2015.02.011","article-title":"m6A RNA methylation is regulated by microRNAs and promotes reprogramming to pluripotency","volume":"16","author":"Chen","year":"2015","journal-title":"Cell Stem Cell"},{"key":"10.1016\/bs.mie.2015.03.012_bb0045","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1002\/anie.201410647","article-title":"High-resolution N6-methyladenosine (m6A) map using photo-crosslinking-assisted m6A sequencing","volume":"54","author":"Chen","year":"2015","journal-title":"Angewandte Chemie International Edition"},{"key":"10.1016\/bs.mie.2015.03.012_bb0050","doi-asserted-by":"crossref","first-page":"17111","DOI":"10.1073\/pnas.0708112104","article-title":"A DNA methylation ratchet governs progression through a bacterial cell cycle","volume":"104","author":"Collier","year":"2007","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"10.1016\/bs.mie.2015.03.012_bb0055","doi-asserted-by":"crossref","first-page":"R79","DOI":"10.1186\/gb-2011-12-8-r79","article-title":"PARalyzer: Definition of RNA binding sites from PAR-CLIP short-read sequence data","volume":"12","author":"Corcoran","year":"2011","journal-title":"Genome Biology"},{"key":"10.1016\/bs.mie.2015.03.012_bb0060","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0005-2787(74)90194-4","article-title":"Methylated bases in DNA from Paramecium aurelia","volume":"374","author":"Cummings","year":"1974","journal-title":"Biochimica et Biophysica Acta"},{"key":"10.1016\/bs.mie.2015.03.012_bb0065","doi-asserted-by":"crossref","first-page":"6322","DOI":"10.1093\/nar\/gkm657","article-title":"Identification of recognition residues for ligation-based detection and quantitation of pseudouridine and N6-methyladenosine","volume":"35","author":"Dai","year":"2007","journal-title":"Nucleic Acids Research"},{"key":"10.1016\/bs.mie.2015.03.012_bb0070","doi-asserted-by":"crossref","first-page":"3971","DOI":"10.1073\/pnas.71.10.3971","article-title":"Identification of methylated nucleosides in messenger RNA from Novikoff hepatoma cells","volume":"71","author":"Desrosiers","year":"1974","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"10.1016\/bs.mie.2015.03.012_bb0075","doi-asserted-by":"crossref","first-page":"4367","DOI":"10.1021\/bi00691a004","article-title":"Characterization of Novikoff hepatoma mRNA methylation and heterogeneity in the methylated 5\u2032 terminus","volume":"14","author":"Desrosiers","year":"1975","journal-title":"Biochemistry"},{"key":"10.1016\/bs.mie.2015.03.012_bb0080","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1021\/bi00622a021","article-title":"Sequence specificity of internal methylation in B77 avian sarcoma virus RNA subunits","volume":"16","author":"Dimock","year":"1977","journal-title":"Biochemistry"},{"key":"10.1016\/bs.mie.2015.03.012_bb0085","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1038\/nprot.2012.148","article-title":"Transcriptome-wide mapping of N6-methyladenosine by m6A-seq based on immunocapturing and massively parallel sequencing","volume":"8","author":"Dominissini","year":"2013","journal-title":"Nature Protocols"},{"key":"10.1016\/bs.mie.2015.03.012_bb0090","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1038\/nature11112","article-title":"Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq","volume":"485","author":"Dominissini","year":"2012","journal-title":"Nature"},{"key":"10.1016\/bs.mie.2015.03.012_bb0095","series-title":"Chromatography and Modification of Nucleosides: Biological roles and function of modification","first-page":"B327","article-title":"Chapter 10 Naturally occurring modified nucleosides in DNA","volume":"Vol. 45B","author":"Ehrlich","year":"1990"},{"key":"10.1016\/bs.mie.2015.03.012_bb0100","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1038\/nbt.2432","article-title":"Genome-wide mapping of methylated adenine residues in pathogenic Escherichia coli using single-molecule real-time sequencing","volume":"30","author":"Fang","year":"2012","journal-title":"Nature Biotechnology"},{"key":"10.1016\/bs.mie.2015.03.012_bb0105","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/nrg3724","article-title":"Gene expression regulation mediated through reversible m6A RNA methylation","volume":"15","author":"Fu","year":"2014","journal-title":"Nature Reviews. Genetics"},{"key":"10.1016\/bs.mie.2015.03.012_bb9000","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1016\/j.cell.2015.04.010","article-title":"N6-Methyldeoxyadenosine Marks Active Transcription Start Sites in Chlamydomonas","volume":"161","author":"Fu","year":"2015","journal-title":"Cell"},{"key":"10.1016\/bs.mie.2015.03.012_bb0110","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1126\/science.1261417","article-title":"m6A mRNA methylation facilitates resolution of na\u00efve pluripotency toward differentiation","volume":"347","author":"Geula","year":"2015","journal-title":"Science"},{"key":"10.1016\/bs.mie.2015.03.012_bb0115","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.cell.2010.03.009","article-title":"Transcriptome-wide identification of RNA-binding protein and microRNA target sites by PAR-CLIP","volume":"141","author":"Hafner","year":"2010","journal-title":"Cell"},{"key":"10.1016\/bs.mie.2015.03.012_bb0120","doi-asserted-by":"crossref","first-page":"5735","DOI":"10.1093\/nar\/18.19.5735","article-title":"Sequence specificity of the human mRNA N6-adenosine methylase in vitro","volume":"18","author":"Harper","year":"1990","journal-title":"Nucleic Acids Research"},{"key":"10.1016\/bs.mie.2015.03.012_bb0125","doi-asserted-by":"crossref","first-page":"2364","DOI":"10.1128\/MCB.6.7.2364","article-title":"Site-specific methylation of adenine in the nuclear genome of a eucaryote, Tetrahymena thermophila","volume":"6","author":"Harrison","year":"1986","journal-title":"Molecular and Cellular Biology"},{"key":"10.1016\/bs.mie.2015.03.012_bb0130","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1128\/JB.135.3.1156-1157.1978","article-title":"Comparative study of DNA methylation in three unicellular eucaryotes","volume":"135","author":"Hattman","year":"1978","journal-title":"Journal of Bacteriology"},{"key":"10.1016\/bs.mie.2015.03.012_bb0135","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1038\/nchembio.482","article-title":"Grand challenge commentary: RNA epigenetics?","volume":"6","author":"He","year":"2010","journal-title":"Nature Chemical Biology"},{"key":"10.1016\/bs.mie.2015.03.012_bb0140","doi-asserted-by":"crossref","first-page":"5667","DOI":"10.1073\/pnas.81.18.5667","article-title":"Mapping of N6-methyladenosine residues in bovine prolactin mRNA","volume":"81","author":"Horowitz","year":"1984","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"10.1016\/bs.mie.2015.03.012_bb0145","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.tig.2012.11.003","article-title":"Reversible RNA adenosine methylation in biological regulation","volume":"29","author":"Jia","year":"2013","journal-title":"Trends in Genetics"},{"key":"10.1016\/bs.mie.2015.03.012_bb0150","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1038\/nchembio.687","article-title":"N6-methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO","volume":"7","author":"Jia","year":"2011","journal-title":"Nature Chemical Biology"},{"key":"10.1016\/bs.mie.2015.03.012_bb0155","doi-asserted-by":"crossref","first-page":"2298","DOI":"10.1128\/MCB.5.9.2298","article-title":"Precise localization of m6A in Rous sarcoma virus RNA reveals clustering of methylation sites: Implications for RNA processing","volume":"5","author":"Kane","year":"1985","journal-title":"Molecular and Cellular Biology"},{"key":"10.1016\/bs.mie.2015.03.012_bb0160","doi-asserted-by":"crossref","first-page":"R25","DOI":"10.1186\/gb-2009-10-3-r25","article-title":"Ultrafast and memory-efficient alignment of short DNA sequences to the human genome","volume":"10","author":"Langmead","year":"2009","journal-title":"Genome Biology"},{"key":"10.1016\/bs.mie.2015.03.012_bb0170","doi-asserted-by":"crossref","first-page":"1848","DOI":"10.1261\/rna.041178.113","article-title":"Probing N6-methyladenosine RNA modification status at single nucleotide resolution in mRNA and long noncoding RNA","volume":"19","author":"Liu","year":"2013","journal-title":"RNA"},{"key":"10.1016\/bs.mie.2015.03.012_bb0175","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1038\/nchembio.1432","article-title":"A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation","volume":"10","author":"Liu","year":"2014","journal-title":"Nature Chemical Biology"},{"key":"10.1016\/bs.mie.2015.03.012_bb0180","doi-asserted-by":"crossref","first-page":"7197","DOI":"10.1128\/IAI.69.12.7197-7204.2001","article-title":"Roles of DNA adenine methylation in regulating bacterial gene expression and virulence","volume":"69","author":"Low","year":"2001","journal-title":"Infection and Immunity"},{"key":"10.1016\/bs.mie.2015.03.012_bb0185","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/0092-8674(94)90156-2","article-title":"SeqA: A negative modulator of replication initiation in E. coli","volume":"77","author":"Lu","year":"1994","journal-title":"Cell"},{"key":"10.1016\/bs.mie.2015.03.012_bb0190","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/S0092-8674(88)90911-7","article-title":"Timing and targeting: The biological functions of Dam methylation in E. coli","volume":"54","author":"Messer","year":"1988","journal-title":"Cell"},{"key":"10.1016\/bs.mie.2015.03.012_bb0195","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1038\/nrm3785","article-title":"The dynamic epitranscriptome: N6-Methyladenosine and gene expression control","volume":"15","author":"Meyer","year":"2014","journal-title":"Nature Reviews Molecular Cell Biology"},{"key":"10.1016\/bs.mie.2015.03.012_bb0200","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1016\/j.cell.2012.05.003","article-title":"Comprehensive analysis of mRNA methylation reveals enrichment in 3\u2032 UTRs and near stop codons","volume":"149","author":"Meyer","year":"2012","journal-title":"Cell"},{"key":"10.1016\/bs.mie.2015.03.012_bb0205","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1099\/00221287-148-1-3","article-title":"2001 Fred Griffith review lecture. Immigration control of DNA in bacteria: Self versus non-self","volume":"148","author":"Murray","year":"2002","journal-title":"Microbiology"},{"key":"10.1016\/bs.mie.2015.03.012_bb0210","doi-asserted-by":"crossref","first-page":"11450","DOI":"10.1093\/nar\/gks891","article-title":"The methylomes of six bacteria","volume":"40","author":"Murray","year":"2012","journal-title":"Nucleic Acids Research"},{"key":"10.1016\/bs.mie.2015.03.012_bb0215","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/0092-8674(88)90186-9","article-title":"The replicative origin of the Escherichia coli chromosome binds to cell membranes only when hemimethylated","volume":"54","author":"Ogden","year":"1988","journal-title":"Cell"},{"key":"10.1016\/bs.mie.2015.03.012_bb0220","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.tibs.2012.12.006","article-title":"N6-Methyl-adenosine modification in messenger and long non-coding RNA","volume":"38","author":"Pan","year":"2013","journal-title":"Trends in Biochemical Sciences"},{"key":"10.1016\/bs.mie.2015.03.012_bb0225","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1038\/cr.2014.3","article-title":"Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase","volume":"24","author":"Ping","year":"2014","journal-title":"Cell Research"},{"key":"10.1016\/bs.mie.2015.03.012_bb0230","doi-asserted-by":"crossref","first-page":"4992","DOI":"10.1073\/pnas.75.10.4992","article-title":"Macronuclear DNA of the hypotrichous ciliate Oxytricha fallax","volume":"75","author":"Rae","year":"1978","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"10.1016\/bs.mie.2015.03.012_bb0235","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1002\/bies.20342","article-title":"N6-Methyladenine: The other methylated base of DNA","volume":"28","author":"Ratel","year":"2006","journal-title":"Bioessays"},{"key":"10.1016\/bs.mie.2015.03.012_bb0240","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1016\/j.cell.2011.11.013","article-title":"Comprehensive genome-wide protein-DNA interactions detected at single-nucleotide resolution","volume":"147","author":"Rhee","year":"2011","journal-title":"Cell"},{"key":"10.1016\/bs.mie.2015.03.012_bb0245","first-page":"35","article-title":"2 Type II Restriction Endonucleases","volume":"25","author":"Roberts","year":"1993","journal-title":"Cold Spring Harbor Monograph Archive"},{"key":"10.1016\/bs.mie.2015.03.012_bb0250","doi-asserted-by":"crossref","first-page":"D234","DOI":"10.1093\/nar\/gkp874","article-title":"REBASE\u2014A database for DNA restriction and modification: Enzymes, genes and genomes","volume":"38","author":"Roberts","year":"2010","journal-title":"Nucleic Acids Research"},{"key":"10.1016\/bs.mie.2015.03.012_bb0255","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1038\/nrg1655","article-title":"DNA methylation and human disease","volume":"6","author":"Robertson","year":"2005","journal-title":"Nature Reviews. Genetics"},{"key":"10.1016\/bs.mie.2015.03.012_bb0260","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1038\/nrg2295","article-title":"Epigenetic events in mammalian germ-cell development: Reprogramming and beyond","volume":"9","author":"Sasaki","year":"2008","journal-title":"Nature Reviews. Genetics"},{"key":"10.1016\/bs.mie.2015.03.012_bb0265","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/0022-2836(77)90110-3","article-title":"Comparison of methylated sequences in messenger RNA and heterogeneous nuclear RNA from mouse L cells","volume":"115","author":"Schibler","year":"1977","journal-title":"Journal of Molecular Biology"},{"key":"10.1016\/bs.mie.2015.03.012_bb0270","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1016\/j.cell.2013.10.047","article-title":"High-resolution mapping reveals a conserved, widespread, dynamic mRNA methylation program in yeast meiosis","volume":"155","author":"Schwartz","year":"2013","journal-title":"Cell"},{"key":"10.1016\/bs.mie.2015.03.012_bb0275","doi-asserted-by":"crossref","first-page":"e156","DOI":"10.1093\/nar\/gku846","article-title":"MACE: Model based analysis of ChIP-exo","volume":"42","author":"Wang","year":"2014","journal-title":"Nucleic Acids Research"},{"key":"10.1016\/bs.mie.2015.03.012_bb0280","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1038\/ncb2902","article-title":"N6-Methyladenosine modification destabilizes developmental regulators in embryonic stem cells","volume":"16","author":"Wang","year":"2014","journal-title":"Nature Cell Biology"},{"key":"10.1016\/bs.mie.2015.03.012_bb0285","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1038\/nature12730","article-title":"N6-Methyladenosine-dependent regulation of messenger RNA stability","volume":"505","author":"Wang","year":"2014","journal-title":"Nature"},{"key":"10.1016\/bs.mie.2015.03.012_bb0290","doi-asserted-by":"crossref","first-page":"1672","DOI":"10.1021\/bi00627a023","article-title":"Nucleotide sequences at the N6-methyladenosine sites of HeLa cell messenger ribonucleic acid","volume":"16","author":"Wei","year":"1977","journal-title":"Biochemistry"},{"key":"10.1016\/bs.mie.2015.03.012_bb0295","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.molcel.2012.10.015","article-title":"ALKBH5 Is a mammalian RNA demethylase that impacts RNA metabolism and mouse fertility","volume":"49","author":"Zheng","year":"2013","journal-title":"Molecular Cell"}],"container-title":["Methods in Enzymology","RNA Modification"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0076687915002384?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0076687915002384?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T22:11:44Z","timestamp":1652911904000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0076687915002384"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"ISBN":["9780128021927"],"references-count":59,"URL":"https:\/\/doi.org\/10.1016\/bs.mie.2015.03.012","relation":{},"ISSN":["0076-6879"],"issn-type":[{"value":"0076-6879","type":"print"}],"subject":[],"published":{"date-parts":[[2015]]}}}