{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T15:16:13Z","timestamp":1778512573835,"version":"3.51.4"},"reference-count":60,"publisher":"Elsevier","isbn-type":[{"value":"9780128168318","type":"print"}],"license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019]]},"DOI":"10.1016\/bs.mie.2019.05.013","type":"book-chapter","created":{"date-parts":[[2019,5,27]],"date-time":"2019-05-27T12:21:43Z","timestamp":1558959703000},"page":"315-337","source":"Crossref","is-referenced-by-count":4,"title":["The use of electrospray ionization mass spectrometry to monitor RNA-ligand interactions"],"prefix":"10.1016","author":[{"given":"Danielle N.","family":"Dremann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine S.","family":"Chow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/bs.mie.2019.05.013_bb0010","doi-asserted-by":"crossref","first-page":"93","DOI":"10.4155\/fmc.09.149","article-title":"Strategies for the design of RNA-binding small molecules","volume":"2","author":"Aboul-ela","year":"2010","journal-title":"Future Medicinal Chemistry"},{"key":"10.1016\/bs.mie.2019.05.013_bb0015","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1002\/pro.2253","article-title":"Sequence-specific determination of protein and peptide concentrations by absorbance at 205nm","volume":"22","author":"Anthis","year":"2013","journal-title":"Protein Science"},{"key":"10.1016\/bs.mie.2019.05.013_bb0020","doi-asserted-by":"crossref","first-page":"8837","DOI":"10.1016\/j.tet.2012.07.001","article-title":"Methods for identifying and characterizing interactions involving RNA","volume":"68","author":"Blakeley","year":"2012","journal-title":"Tetrahedron"},{"key":"10.1016\/bs.mie.2019.05.013_bb0025","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1002\/jmr.622","article-title":"Measurement of dissociation constants of inhibitors binding to Src SH2 domain protein by non-covalent electrospray ionization mass spectrometry","volume":"16","author":"Bligh","year":"2003","journal-title":"Journal of Molecular Recognition"},{"key":"10.1016\/bs.mie.2019.05.013_bb0030","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1038\/nsmb1271","article-title":"Structural basis for aminoglycoside inhibition of bacterial ribosome recycling","volume":"14","author":"Borovinskaya","year":"2007","journal-title":"Nature Structural & Molecular Biology"},{"key":"10.1016\/bs.mie.2019.05.013_bb0035","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1093\/embo-reports\/kve062","article-title":"Structural basis for selectivity and toxicity of ribosomal antibiotics","volume":"2","author":"B\u00f6ttger","year":"2001","journal-title":"EMBO Reports"},{"key":"10.1016\/bs.mie.2019.05.013_bb0040","doi-asserted-by":"crossref","first-page":"3404","DOI":"10.1021\/jo00795a005","article-title":"The 9-fluorenylmethoxycarbonyl amino-protecting group","volume":"37","author":"Carpino","year":"1972","journal-title":"Journal of Organic Chemistry"},{"key":"10.1016\/bs.mie.2019.05.013_bb0045","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1126\/science.285.5436.2095","article-title":"X-ray crystal structures of 70S ribosome functional complexes","volume":"285","author":"Cate","year":"1999","journal-title":"Science"},{"key":"10.1016\/bs.mie.2019.05.013_bb0050","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1021\/cr960415w","article-title":"A structural basis for RNA-ligand interactions","volume":"97","author":"Chow","year":"1997","journal-title":"Chemical Reviews"},{"key":"10.1016\/bs.mie.2019.05.013_bb0055","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1021\/cb7002225","article-title":"Combined approaches to site-specific modification of RNA","volume":"3","author":"Chow","year":"2008","journal-title":"ACS Chemical Biology"},{"key":"10.1016\/bs.mie.2019.05.013_bb0060","series-title":"Electrospray and MALDI mass spectrometry: Fundamentals, instrumentational practicalities, and biological applications","author":"Cole","year":"2010"},{"key":"10.1016\/bs.mie.2019.05.013_bb0065","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1016\/j.chembiol.2016.05.021","article-title":"The emerging role of RNA as a therapeutic target for small molecules","volume":"23","author":"Connelly","year":"2016","journal-title":"Cell Chemical Biology"},{"key":"10.1016\/bs.mie.2019.05.013_bb0070","doi-asserted-by":"crossref","first-page":"11931","DOI":"10.1073\/pnas.0900629106","article-title":"Simultaneous recognition of HIV-1 TAR RNA bulge and loop sequences by cyclic peptide mimics of Tat protein","volume":"106","author":"Davidson","year":"2009","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"10.1016\/bs.mie.2019.05.013_bb0075","doi-asserted-by":"crossref","first-page":"4486","DOI":"10.1016\/j.bmc.2016.07.050","article-title":"The development of peptide ligands that target helix 69 rRNA of bacterial ribosomes","volume":"24","author":"Dremann","year":"2016","journal-title":"Bioorganic and Medicinal Chemistry"},{"key":"10.1016\/bs.mie.2019.05.013_bb0080","series-title":"Handbook of biochemistry and molecular biology","author":"Fasman","year":"1975"},{"key":"10.1016\/bs.mie.2019.05.013_bb0085","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1126\/science.2675315","article-title":"Electrospray ionization for mass spectrometry of large biomolecules","volume":"246","author":"Fenn","year":"1989","journal-title":"Science"},{"key":"10.1016\/bs.mie.2019.05.013_bb0090","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.4161\/15476286.2014.992280","article-title":"The identification and characterization of non-coding and coding RNAs and their modified nucleosides by mass spectrometry","volume":"11","author":"Gaston","year":"2014","journal-title":"RNA Biology"},{"key":"10.1016\/bs.mie.2019.05.013_bb0095","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1021\/ja983131x","article-title":"Targeted site-specific gas-phase cleavage of oligoribonucleotides. Application in mass spectrometry-based identification of ligand binding sites","volume":"121","author":"Griffey","year":"1999","journal-title":"Journal of the American Chemical Society"},{"key":"10.1016\/bs.mie.2019.05.013_bb0100","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1021\/cb200447r","article-title":"Recent advances in developing small molecules targeting RNA","volume":"7","author":"Guan","year":"2012","journal-title":"ACS Chemical Biology"},{"key":"10.1016\/bs.mie.2019.05.013_bb0105","first-page":"33","article-title":"RNA oligonucleotide synthesis via 5\u2032-silyl-2\u2032-orthoester chemistry","volume":"288","author":"Hartsel","year":"2005","journal-title":"Methods in Molecular Biology"},{"key":"10.1016\/bs.mie.2019.05.013_bb0110","doi-asserted-by":"crossref","first-page":"3436","DOI":"10.1021\/ac990262n","article-title":"Multiplexed screening of neutral mass-tagged RNA targets against ligand libraries with electrospray ionization FTICR MS: A paradigm for high-throughput affinity screening","volume":"71","author":"Hofstadler","year":"1999","journal-title":"Analytical Chemistry"},{"key":"10.1016\/bs.mie.2019.05.013_bb0115","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1016\/j.bmc.2012.12.048","article-title":"Selection of heptapeptides that bind helix 69 of bacterial 23S ribosomal RNA","volume":"21","author":"Kaur","year":"2013","journal-title":"Bioorganic and Medicinal Chemistry"},{"key":"10.1016\/bs.mie.2019.05.013_bb0120","doi-asserted-by":"crossref","first-page":"2700","DOI":"10.1016\/j.bmc.2017.06.052","article-title":"Therapeutic peptides: Historical perspectives, current development trends, and future directions","volume":"26","author":"Lau","year":"2018","journal-title":"Bioorganic and Medicinal Chemistry"},{"key":"10.1016\/bs.mie.2019.05.013_bb0125","doi-asserted-by":"crossref","first-page":"8299","DOI":"10.1021\/bi900982t","article-title":"Selection of peptides that target the aminoacyl-tRNA site of bacterial 16S ribosomal RNA","volume":"48","author":"Li","year":"2009","journal-title":"Biochemistry"},{"key":"10.1016\/bs.mie.2019.05.013_bb0130","doi-asserted-by":"crossref","first-page":"2149","DOI":"10.1021\/ja00897a025","article-title":"Solid phase peptide synthesis. 1. Synthesis of a tetrapeptide","volume":"85","author":"Merrifield","year":"1963","journal-title":"Journal of the American Chemical Society"},{"key":"10.1016\/bs.mie.2019.05.013_bb0135","doi-asserted-by":"crossref","first-page":"8783","DOI":"10.1093\/nar\/15.21.8783","article-title":"Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates","volume":"15","author":"Milligan","year":"1987","journal-title":"Nucleic Acids Research"},{"key":"10.1016\/bs.mie.2019.05.013_bb0140","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1038\/327389a0","article-title":"Interaction of antibiotics with functional sites in 16S ribosomal RNA","volume":"327","author":"Moazed","year":"1987","journal-title":"Nature"},{"key":"10.1016\/bs.mie.2019.05.013_bb0145","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.tibs.2005.04.006","article-title":"The ribosome revealed","volume":"30","author":"Moore","year":"2005","journal-title":"Trends in Biochemical Sciences"},{"key":"10.1016\/bs.mie.2019.05.013_bb0150","doi-asserted-by":"crossref","first-page":"3347","DOI":"10.1093\/nar\/21.15.3347","article-title":"Ion stability of nucleic acids in infrared matrix-assisted laser desorption\/ionization mass spectrometry","volume":"21","author":"Nordhoff","year":"1993","journal-title":"Nucleic Acids Research"},{"key":"10.1016\/bs.mie.2019.05.013_bb0155","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1006\/jmbi.1996.0737","article-title":"Mapping to nucleotide resolution of pseudouridine residues in large subunit ribosomal RNAs from representative eukaryotes, prokaryotes, archaebacteria, mitochondria and chloroplasts","volume":"266","author":"Ofengand","year":"1997","journal-title":"Journal of Molecular Biology"},{"key":"10.1016\/bs.mie.2019.05.013_bb0160","doi-asserted-by":"crossref","first-page":"2768","DOI":"10.1139\/v78-457","article-title":"The synthesis of oligoribonucleotides. II. The use of silyl protecting groups in nucleoside and nucleotide chemistry VII","volume":"56","author":"Ogilvie","year":"1978","journal-title":"Canadian Journal of Chemistry"},{"key":"10.1016\/bs.mie.2019.05.013_bb0165","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S1359-6446(04)03285-4","article-title":"Antibiotics: Where did we go wrong?","volume":"10","author":"Overbye","year":"2005","journal-title":"Drug Discovery Today"},{"key":"10.1016\/bs.mie.2019.05.013_bb0170","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.mib.2003.08.003","article-title":"Why is big Pharma getting out of antibacterial drug discovery?","volume":"6","author":"Projan","year":"2003","journal-title":"Current Opinion in Microbiology"},{"key":"10.1016\/bs.mie.2019.05.013_bb0175","doi-asserted-by":"crossref","first-page":"230","DOI":"10.2174\/187152607782110040","article-title":"Strategic paradigm shifts in the antimicrobial drug discovery process of the 21st century","volume":"7","author":"Quadri","year":"2007","journal-title":"Infectious Disorders Drug Targets"},{"key":"10.1016\/bs.mie.2019.05.013_bb0180","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1006\/jmbi.1996.0526","article-title":"RNA sequence determinants for aminoglycoside binding to an A-site rRNA model oligonucleotide","volume":"262","author":"Recht","year":"1996","journal-title":"Journal of Molecular Biology"},{"key":"10.1016\/bs.mie.2019.05.013_bb0185","doi-asserted-by":"crossref","first-page":"3787","DOI":"10.1016\/S0040-4039(00)96384-6","article-title":"Solid-phase synthesis of protected peptide-fragments using a trialkoxy-diphenyl-methylester resin","volume":"28","author":"Rink","year":"1987","journal-title":"Tetrahedron Letters"},{"key":"10.1016\/bs.mie.2019.05.013_bb0190","doi-asserted-by":"crossref","first-page":"12095","DOI":"10.1002\/anie.201206000","article-title":"Ligand- and pH-induced changes of RNA domain helix 69 revealed by 2-aminopurine fluorescence","volume":"51","author":"Sakakibara","year":"2012","journal-title":"Angewandte Chemie International Edition"},{"key":"10.1016\/bs.mie.2019.05.013_bb0195","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1006\/abio.2000.4550","article-title":"Measuring dissociation constants of RNA and aminoglycoside antibiotics by electrospray ionization mass spectrometry","volume":"280","author":"Sannes-Lowery","year":"2000","journal-title":"Analytical Biochemistry"},{"key":"10.1016\/bs.mie.2019.05.013_bb0200","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1021\/ac60214a047","article-title":"Smoothing and differentiation of data by simplified least squares procedures","volume":"36","author":"Savitzky","year":"1964","journal-title":"Analytical Chemistry"},{"key":"10.1016\/bs.mie.2019.05.013_bb0205","doi-asserted-by":"crossref","first-page":"1254","DOI":"10.1002\/anie.201610836","article-title":"Native top-down mass spectrometry of TAR RNA in complexes with wild-type tat peptide for binding site mapping","volume":"56","author":"Schneeberger","year":"2017","journal-title":"Angewandte Chemie International Edition"},{"key":"10.1016\/bs.mie.2019.05.013_bb0210","series-title":"Spectrometric identifcation of organic compounds","author":"Silverstein","year":"2005"},{"key":"10.1016\/bs.mie.2019.05.013_bb0215","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1016\/j.febslet.2004.11.053","article-title":"On the structural basis of peptide-bond formation and antibiotic resistance from atomic structures of the large ribosomal subunit","volume":"579","author":"Steitz","year":"2005","journal-title":"FEBS Letters"},{"key":"10.1016\/bs.mie.2019.05.013_bb0220","doi-asserted-by":"crossref","first-page":"4542","DOI":"10.1021\/ac00120a018","article-title":"Noncovalent protein\u2014Oligonucleotide interactions monitored by matrix-assisted laser desorption\/ionization mass spectrometry","volume":"67","author":"Tang","year":"1995","journal-title":"Analytical Chemistry"},{"key":"10.1016\/bs.mie.2019.05.013_bb0225","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1021\/cr0681546","article-title":"Targeting RNA with small molecules","volume":"108","author":"Thomas","year":"2008","journal-title":"Chemical Reviews"},{"key":"10.1016\/bs.mie.2019.05.013_bb0230","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1002\/cbic.200300680","article-title":"Targeting RNA with small molecules","volume":"4","author":"Tor","year":"2003","journal-title":"Chembiochem"},{"key":"10.1016\/bs.mie.2019.05.013_bb0235","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.cell.2005.02.005","article-title":"Structures of MLSBK antibiotics bound to mutated large ribosomal subunits provide a structural explanation for resistance","volume":"121","author":"Tu","year":"2005","journal-title":"Cell"},{"key":"10.1016\/bs.mie.2019.05.013_bb0240","doi-asserted-by":"crossref","first-page":"1402","DOI":"10.1016\/j.jasms.2006.06.009","article-title":"Mapping noncovalent ligand binding to stemloop domains of the HIV-1 packaging signal by tandem mass spectrometry","volume":"17","author":"Turner","year":"2006","journal-title":"Journal of the American Society for Mass Spectrometry"},{"key":"10.1016\/bs.mie.2019.05.013_bb0245","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1002\/open.201700143","article-title":"Interactions of protonated guanidine and guanidine derivatives with multiply deprotonated RNA probed by electrospray ionization and collisionally activated dissociation","volume":"6","author":"Vusurovic","year":"2017","journal-title":"ChemistryOpen"},{"key":"10.1016\/bs.mie.2019.05.013_bb0250","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.ymeth.2018.10.015","article-title":"Chemical probing for examining the structure of modified RNAs and ligand binding to RNA","volume":"156","author":"Waduge","year":"2019","journal-title":"Methods"},{"key":"10.1016\/bs.mie.2019.05.013_bb0255","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1128\/JB.58.2.229-237.1949","article-title":"The in vivo activity of neomycin","volume":"58","author":"Waksman","year":"1949","journal-title":"Journal of Bacteriology"},{"key":"10.1016\/bs.mie.2019.05.013_bb0260","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1126\/science.109.2830.305","article-title":"Neomycin, a new antibiotic active against streptomycin-resistant bacteria, including tuberculosis organisms","volume":"109","author":"Waksman","year":"1949","journal-title":"Science"},{"key":"10.1016\/bs.mie.2019.05.013_bb0265","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1172\/JCI102182","article-title":"Neomycin-production and antibiotic properties","volume":"28","author":"Waksman","year":"1949","journal-title":"The Journal of Clinical Investigation"},{"key":"10.1016\/bs.mie.2019.05.013_bb0270","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1038\/nsmb.2360","article-title":"Allosteric control of the ribosome by small-molecule antibiotics","volume":"19","author":"Wang","year":"2012","journal-title":"Nature Structural & Molecular Biology"},{"key":"10.1016\/bs.mie.2019.05.013_bb0275","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1038\/nrd.2018.93","article-title":"Principles for targeting RNA with drug-like small molecules","volume":"17","author":"Warner","year":"2018","journal-title":"Nature Reviews Drug Discovery"},{"key":"10.1016\/bs.mie.2019.05.013_bb0280","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1039\/C5AN01797A","article-title":"Mass spectrometry of modified RNAs: Recent developments","volume":"141","author":"Wetzel","year":"2016","journal-title":"The Analyst"},{"key":"10.1016\/bs.mie.2019.05.013_bb0285","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a011536","article-title":"The structure and function of the eukaryotic ribosome","volume":"4","author":"Wilson","year":"2012","journal-title":"Cold Spring Harbor Perspectives in Biology"},{"key":"10.1016\/bs.mie.2019.05.013_bb0290","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.heares.2011.05.008","article-title":"New developments in aminoglycoside therapy and ototoxicity","volume":"281","author":"Xie","year":"2011","journal-title":"Hearing Research"},{"key":"10.1016\/bs.mie.2019.05.013_bb0295","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1016\/j.jasms.2010.01.002","article-title":"Elucidating the site of protein-ATP binding by top-down mass spectrometry","volume":"21","author":"Yin","year":"2010","journal-title":"Journal of the American Society for Mass Spectrometry"},{"key":"10.1016\/bs.mie.2019.05.013_bb0300","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1016\/j.jasms.2008.05.012","article-title":"Mass spectrometry of protein-ligand complexes: Enhanced gas-phase stability of ribonuclease-nucleotide complexes","volume":"19","author":"Yin","year":"2008","journal-title":"Journal of the American Society for Mass Spectrometry"},{"key":"10.1016\/bs.mie.2019.05.013_bb0305","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1126\/science.1060089","article-title":"Crystal structure of the ribosome at 5.5 \u00c5 resolution","volume":"292","author":"Yusupov","year":"2001","journal-title":"Science"}],"container-title":["Methods in Enzymology","RNA Recognition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0076687919301727?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0076687919301727?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,12,15]],"date-time":"2020-12-15T20:51:08Z","timestamp":1608065468000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0076687919301727"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"ISBN":["9780128168318"],"references-count":60,"URL":"https:\/\/doi.org\/10.1016\/bs.mie.2019.05.013","relation":{},"ISSN":["0076-6879"],"issn-type":[{"value":"0076-6879","type":"print"}],"subject":[],"published":{"date-parts":[[2019]]}}}