{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T04:13:01Z","timestamp":1766117581999,"version":"3.48.0"},"reference-count":38,"publisher":"Elsevier","isbn-type":[{"type":"print","value":"9780443429590"}],"license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-20-CE11-0018"],"award-info":[{"award-number":["ANR-20-CE11-0018"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025]]},"DOI":"10.1016\/bs.mie.2025.08.028","type":"book-chapter","created":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T14:59:13Z","timestamp":1758207553000},"page":"365-396","source":"Crossref","is-referenced-by-count":0,"title":["MexB\/inhibitor complexes preparation for crystallography and cryo-EM, comparison and possible pitfalls"],"prefix":"10.1016","member":"78","reference":[{"key":"10.1016\/bs.mie.2025.08.028_bib1","doi-asserted-by":"crossref","first-page":"5479","DOI":"10.1021\/acs.chemrev.1c00055","article-title":"Structure, assembly, and function of tripartite efflux and type 1 secretion systems in gram-negative bacteria","volume":"121","author":"Alav","year":"2021","journal-title":"Chemical Reviews"},{"key":"10.1016\/bs.mie.2025.08.028_bib2","doi-asserted-by":"crossref","first-page":"e02492","DOI":"10.1128\/mbio.02492-23","article-title":"Bacterial efflux pump modulators prevent bacterial growth in macrophages and under broth conditions that mimic the host environment","volume":"14","author":"Allgood","year":"2023","journal-title":"mBio"},{"key":"10.1016\/bs.mie.2025.08.028_bib3","doi-asserted-by":"crossref","first-page":"180","DOI":"10.3390\/antibiotics12010180","article-title":"Update on the discovery of efflux pump inhibitors against critical priority gram-negative bacteria","volume":"12","author":"Compagne","year":"2023","journal-title":"Antibiotics"},{"key":"10.1016\/bs.mie.2025.08.028_bib4","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1038\/s41579-022-00820-y","article-title":"Molecular mechanisms of antibiotic resistance revisited","volume":"21","author":"Darby","year":"2023","journal-title":"Nature Reviews. Microbiology"},{"key":"10.1016\/bs.mie.2025.08.028_bib5","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms10731","article-title":"Tripartite assembly of RND multidrug efflux pumps","volume":"7","author":"Daury","year":"2016","journal-title":"Nature Communications"},{"key":"10.1016\/bs.mie.2025.08.028_bib6","doi-asserted-by":"crossref","first-page":"4669","DOI":"10.1021\/acs.chemrev.6b00690","article-title":"Nanodiscs in membrane biochemistry and biophysics","volume":"117","author":"Denisov","year":"2017","journal-title":"Chemical Reviews"},{"key":"10.1016\/bs.mie.2025.08.028_bib7","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.tim.2015.01.010","article-title":"Assembly and operation of bacterial tripartite multidrug efflux pumps","volume":"23","author":"Du","year":"2015","journal-title":"Trends in Microbiology"},{"key":"10.1016\/bs.mie.2025.08.028_bib8","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1107\/S0907444904019158","article-title":"Coot: Model-building tools for molecular graphics","volume":"60","author":"Emsley","year":"2004","journal-title":"Acta Crystallographica. Section D, Biological Crystallography"},{"key":"10.1016\/bs.mie.2025.08.028_bib9","doi-asserted-by":"crossref","first-page":"4948","DOI":"10.1038\/s41467-020-18770-5","article-title":"Antibiotic export by MexB multidrug efflux transporter is allosterically controlled by a MexA-OprM chaperone-like complex","volume":"11","author":"Glavier","year":"2020","journal-title":"Nature Communications"},{"key":"10.1016\/bs.mie.2025.08.028_bib10","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1002\/pro.3235","article-title":"UCSF ChimeraX: Meeting modern challenges in visualization and analysis","volume":"27","author":"Goddard","year":"2018","journal-title":"Protein Science"},{"key":"10.1016\/bs.mie.2025.08.028_bib11","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1107\/S0907444909047337","article-title":"XDS","volume":"66","author":"Kabsch","year":"2010","journal-title":"Acta Crystallographica. Section D, Biological Crystallography"},{"key":"10.1016\/bs.mie.2025.08.028_bib12","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1111\/nyas.14239","article-title":"AcrB: A mean, keen, drug efflux machine","volume":"1459","author":"Kobylka","year":"2020","journal-title":"Annals of the New York Academy of Sciences"},{"key":"10.1016\/bs.mie.2025.08.028_bib13","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2021.665759","article-title":"The building blocks of antimicrobial resistance in pseudomonas aeruginosa: Implications for current resistance-breaking therapies","volume":"11","author":"Langendonk","year":"2021","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"key":"10.1016\/bs.mie.2025.08.028_bib14","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1128\/CMR.00117-14","article-title":"The challenge of efflux-mediated antibiotic resistance in gram-negative bacteria","volume":"28","author":"Li","year":"2015","journal-title":"Clinical Microbiology Reviews"},{"key":"10.1016\/bs.mie.2025.08.028_bib15","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1107\/S2059798319011471","article-title":"Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix","volume":"75","author":"Liebschner","year":"2019","journal-title":"Acta Crystallographica Section D, Structural Biology"},{"key":"10.1016\/bs.mie.2025.08.028_bib16","doi-asserted-by":"crossref","first-page":"3322","DOI":"10.1128\/AAC.44.12.3322-3327.2000","article-title":"Substrate specificities of MexAB-OprM, MexCD-OprJ, and MexXY-OprM efflux pumps in Pseudomonas aeruginosa","volume":"44","author":"Masuda","year":"2000","journal-title":"Antimicrobial Agents and Chemotherapy"},{"key":"10.1016\/bs.mie.2025.08.028_bib17","series-title":"Bacterial secretion systems","first-page":"415","article-title":"Starting with an integral membrane protein project for structural biology: Production, purification, detergent quantification, and buffer optimization\u2014case study of the exporter CNTI from pseudomonas aeruginosa","author":"M\u00e9gret-Cavalier","year":"2024"},{"key":"10.1016\/bs.mie.2025.08.028_bib18","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.02785-20","article-title":"Predictive rules of efflux inhibition and avoidance in pseudomonas aeruginosa","volume":"12","author":"Mehla","year":"2021","journal-title":"mBio"},{"key":"10.1016\/bs.mie.2025.08.028_bib19","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial antimicrobial resistance in 2019: A systematic analysis","volume":"399","author":"Murray","year":"2022","journal-title":"The Lancet"},{"key":"10.1016\/bs.mie.2025.08.028_bib20","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/nature12300","article-title":"Structural basis for the inhibition of bacterial multidrug exporters","volume":"500","author":"Nakashima","year":"2013","journal-title":"Nature"},{"key":"10.1016\/bs.mie.2025.08.028_bib21","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1038\/nature10641","article-title":"Structures of the multidrug exporter AcrB reveal a proximal multisite drug-binding pocket","volume":"480","author":"Nakashima","year":"2011","journal-title":"Nature"},{"key":"10.1016\/bs.mie.2025.08.028_bib22","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1038\/nmeth.4079","article-title":"Covalently circularized nanodiscs for studying membrane proteins and viral entry","volume":"14","author":"Nasr","year":"2017","journal-title":"Nature Methods"},{"key":"10.1016\/bs.mie.2025.08.028_bib23","unstructured":"O\u2019Neill, J. (2014). Antimicrobial resistance: tackling a crisis for the health and wealth of nations."},{"key":"10.1016\/bs.mie.2025.08.028_bib24","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nmeth.4169","article-title":"cryoSPARC: Algorithms for rapid unsupervised cryo-EM structure determination","volume":"14","author":"Punjani","year":"2017","journal-title":"Nature Methods"},{"key":"10.1016\/bs.mie.2025.08.028_bib25","series-title":"Methods in enzymology","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/S0076-6879(09)64011-8","article-title":"Reconstitution of membrane proteins in phospholipid bilayer nanodiscs","author":"Ritchie","year":"2009"},{"key":"10.1016\/bs.mie.2025.08.028_bib26","doi-asserted-by":"crossref","first-page":"4359","DOI":"10.1038\/s41598-019-40232-2","article-title":"Crystal structures of multidrug efflux pump MexB bound with high-molecular-mass compounds","volume":"9","author":"Sakurai","year":"2019","journal-title":"Scientific Reports"},{"key":"10.1016\/bs.mie.2025.08.028_bib27","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.jmb.2009.04.001","article-title":"Crystal structure of the multidrug exporter MexB from pseudomonas aeruginosa","volume":"389","author":"Sennhauser","year":"2009","journal-title":"Journal of Molecular Biology"},{"key":"10.1016\/bs.mie.2025.08.028_bib28","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/S1473-3099(17)30753-3","article-title":"Discovery, research, and development of new antibiotics: The WHO priority list of antibiotic-resistant bacteria and tuberculosis","volume":"18","author":"Tacconelli","year":"2018","journal-title":"The Lancet Infectious Diseases"},{"key":"10.1016\/bs.mie.2025.08.028_bib29","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1107\/S090744490705024X","article-title":"Iterative model building, structure refinement and density modification with the PHENIX AutoBuild wizard","volume":"64","author":"Terwilliger","year":"2008","journal-title":"Acta Crystallographica. Section D, Biological Crystallography"},{"key":"10.1016\/bs.mie.2025.08.028_bib30","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.1038\/s41467-019-09463-9","article-title":"Structures of the wild-type MexAB\u2013OprM tripartite pump reveal its complex formation and drug efflux mechanism","volume":"10","author":"Tsutsumi","year":"2019","journal-title":"Nature Communications"},{"key":"10.1016\/bs.mie.2025.08.028_bib31","doi-asserted-by":"crossref","first-page":"D439","DOI":"10.1093\/nar\/gkab1061","article-title":"AlphaFold protein structure database: Massively expanding the structural coverage of protein-sequence space with high-accuracy models","volume":"50","author":"Varadi","year":"2022","journal-title":"Nucleic Acids Research"},{"key":"10.1016\/bs.mie.2025.08.028_bib32","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1038\/s44321-023-00007-9","article-title":"Pyridylpiperazine efflux pump inhibitor boosts in vivo antibiotic efficacy against K. Pneumoniae","volume":"16","author":"Vieira Da Cruz","year":"2023","journal-title":"EMBO Molecular Medicine"},{"key":"10.1016\/bs.mie.2025.08.028_bib33","article-title":"An allosteric transport mechanism for the AcrAB-TolC multidrug efflux pump","volume":"6","author":"Wang","year":"2017","journal-title":"eLife"},{"key":"10.1016\/bs.mie.2025.08.028_bib34","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2015.00327","article-title":"Structural basis of RND-type multidrug exporters","volume":"6","author":"Yamaguchi","year":"2015","journal-title":"Frontiers in Microbiology"},{"key":"10.1016\/bs.mie.2025.08.028_bib35","doi-asserted-by":"crossref","first-page":"6804","DOI":"10.1128\/JB.187.19.6804-6815.2005","article-title":"A periplasmic drug-binding site of the AcrB multidrug efflux pump: A crystallographic and site-directed mutagenesis study","volume":"187","author":"Yu","year":"2005","journal-title":"Journal of Bacteriology"},{"key":"10.1016\/bs.mie.2025.08.028_bib36","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1021\/acs.accounts.0c00843","article-title":"Multidrug efflux pumps and the two-faced janus of substrates and inhibitors","volume":"54","author":"Zgurskaya","year":"2021","journal-title":"Accounts of Chemical Research"},{"key":"10.1016\/bs.mie.2025.08.028_bib37","doi-asserted-by":"crossref","DOI":"10.1038\/s41587-025-02654-4","article-title":"Deep-learning-based single-domain and multidomain protein structure prediction with D-I-TASSER","author":"Zheng","year":"2025","journal-title":"Nature Biotechnology"},{"key":"10.1016\/bs.mie.2025.08.028_bib38","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1038\/s41467-017-02493-1","article-title":"Multiple entry pathways within the efflux transporter AcrB contribute to multidrug recognition","volume":"9","author":"Zwama","year":"2018","journal-title":"Nature Communications"}],"container-title":["Methods in Enzymology","Bacterial Efflux Systems"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0076687925003441?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0076687925003441?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T03:21:53Z","timestamp":1766114513000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0076687925003441"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"ISBN":["9780443429590"],"references-count":38,"URL":"https:\/\/doi.org\/10.1016\/bs.mie.2025.08.028","relation":{},"ISSN":["0076-6879"],"issn-type":[{"type":"print","value":"0076-6879"}],"subject":[],"published":{"date-parts":[[2025]]}}}