{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T17:46:08Z","timestamp":1782409568808,"version":"3.54.5"},"reference-count":40,"publisher":"Elsevier","isbn-type":[{"value":"9780443432248","type":"print"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1016\/bs.mie.2026.05.026","type":"book-chapter","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T11:16:06Z","timestamp":1779794166000},"page":"253-284","source":"Crossref","is-referenced-by-count":0,"title":["Efficient labeling of pathogenic fungal chitin with bioorthogonal probes"],"prefix":"10.1016","author":[{"given":"Caroline","family":"Williams","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen C.","family":"Stathoulopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Blanda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bella R.","family":"Carnahan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine L.","family":"Grimes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"46","key":"10.1016\/bs.mie.2026.05.026_bib1","doi-asserted-by":"crossref","first-page":"15046","DOI":"10.1021\/ja044996f","article-title":"A strain-promoted [3 + 2] azide-alkyne cycloaddition for covalent modification of biomolecules in living systems","volume":"126","author":"Agard","year":"2004","journal-title":"Journal of the American Chemical Society"},{"issue":"41","key":"10.1016\/bs.mie.2026.05.026_bib2","doi-asserted-by":"crossref","first-page":"13518","DOI":"10.1021\/ja8053805","article-title":"Tetrazine ligation: Fast bioconjugation based on inverse-electron-demand Diels-Alder reactivity","volume":"130","author":"Blackman","year":"2008","journal-title":"Journal of the American Chemical Society"},{"issue":"9","key":"10.1016\/bs.mie.2026.05.026_bib3","doi-asserted-by":"crossref","first-page":"3988","DOI":"10.1073\/pnas.0911247107","article-title":"Targeted metabolic labeling of yeast N-glycans with unnatural sugars","volume":"107","author":"Breidenbach","year":"2010","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"10","key":"10.1016\/bs.mie.2026.05.026_bib4","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.1021\/acschembio.1c00268","article-title":"Protected N-acetyl muramic acid probes improve bacterial peptidoglycan incorporation via metabolic labeling","volume":"16","author":"Brown","year":"2021","journal-title":"ACS Chemical Biology"},{"key":"10.1016\/bs.mie.2026.05.026_bib5","unstructured":"CDC. (2019). Antibiotic resistance threats in the United States. Retrieved from https:\/\/www.cdc.gov\/antimicrobial-resistance\/data-research\/threats\/index.html."},{"issue":"3","key":"10.1016\/bs.mie.2026.05.026_bib6","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1128\/MMBR.00032-07","article-title":"Candida albicans cell wall proteins","volume":"72","author":"Chaffin","year":"2008","journal-title":"Microbiology and Molecular Biology Reviews: MMBR"},{"issue":"12","key":"10.1016\/bs.mie.2026.05.026_bib7","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1016\/S1473-3099(18)30597-8","article-title":"Multiple introductions and subsequent transmission of multidrug-resistant Candida auris in the USA: A molecular epidemiological survey","volume":"18","author":"Chow","year":"2018","journal-title":"The Lancet Infectious Diseases"},{"issue":"5","key":"10.1016\/bs.mie.2026.05.026_bib8","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.02347-19","article-title":"Remasking of Candida albicans beta-glucan in response to environmental pH Is regulated by quorum sensing","volume":"10","author":"Cottier","year":"2019","journal-title":"mBio"},{"issue":"4","key":"10.1016\/bs.mie.2026.05.026_bib9","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00986-15","article-title":"Cell wall remodeling enzymes modulate fungal cell wall elasticity and osmotic stress resistance","volume":"6","author":"Ene","year":"2015","journal-title":"mBio"},{"key":"10.1016\/bs.mie.2026.05.026_bib10","doi-asserted-by":"crossref","first-page":"2993","DOI":"10.3389\/fmicb.2019.02993","article-title":"The fungal cell wall: Candida, cryptococcus, and aspergillus species","volume":"10","author":"Garcia-Rubio","year":"2019","journal-title":"Frontiers in Microbiology"},{"issue":"17","key":"10.1016\/bs.mie.2026.05.026_bib11","doi-asserted-by":"crossref","first-page":"3639","DOI":"10.1021\/ol0610946","article-title":"Click chemistry as a reliable method for the high-density postsynthetic functionalization of alkyne-modified DNA","volume":"8","author":"Gierlich","year":"2006","journal-title":"Organic Letters"},{"issue":"25","key":"10.1016\/bs.mie.2026.05.026_bib12","doi-asserted-by":"crossref","first-page":"14846","DOI":"10.1073\/pnas.2335201100","article-title":"A metabolic labeling approach toward proteomic analysis of mucin-type O-linked glycosylation","volume":"100","author":"Hang","year":"2003","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"10","key":"10.1016\/bs.mie.2026.05.026_bib13","doi-asserted-by":"crossref","first-page":"6817","DOI":"10.1021\/jacs.3c13644","article-title":"Minimalist tetrazine N-acetyl muramic acid probes for rapid and efficient labeling of commensal and pathogenic peptidoglycans in living bacterial culture and during macrophage invasion","volume":"146","author":"Hillman","year":"2024","journal-title":"Journal of the American Chemical Society"},{"key":"10.1016\/bs.mie.2026.05.026_bib14","series-title":"The bacterial cell wall: Methods and protocols","first-page":"1","article-title":"Bioorthogonal labeling and click-chemistry-based visualization of the tannerella forsythia cell wall","author":"Hyland","year":"2024"},{"issue":"36","key":"10.1016\/bs.mie.2026.05.026_bib15","doi-asserted-by":"crossref","first-page":"15332","DOI":"10.1073\/pnas.0907864106","article-title":"Metabolic labeling and direct imaging of choline phospholipids in vivo","volume":"106","author":"Jao","year":"2009","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"10.1016\/bs.mie.2026.05.026_bib16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/mmy.39.1.1.8-0","article-title":"Molecular organization of the cell wall of Candida albicans","volume":"39 1","author":"Klis","year":"2001","journal-title":"Medical Mycology: Official Publication of the International Society for Human and Animal Mycology"},{"key":"10.1016\/bs.mie.2026.05.026_bib17","doi-asserted-by":"crossref","first-page":"7494","DOI":"10.1038\/srep07494","article-title":"From cells to muropeptide structures in 24h: Peptidoglycan mapping by UPLC-MS","volume":"4","author":"Kuhner","year":"2014","journal-title":"Scientific Reports"},{"key":"10.1016\/bs.mie.2026.05.026_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.tcsw.2020.100047","article-title":"Scalar nanostructure of the Candida albicans cell wall; a molecular, cellular and ultrastructural analysis and interpretation","volume":"6","author":"Lenardon","year":"2020","journal-title":"Cell Surface"},{"key":"10.1016\/bs.mie.2026.05.026_bib19","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms15015","article-title":"Metabolic labelling of the carbohydrate core in bacterial peptidoglycan and its applications","volume":"8","author":"Liang","year":"2017","journal-title":"Nature Communications"},{"key":"10.1016\/bs.mie.2026.05.026_bib20","article-title":"Evaluation and comparison of Candida albicans vs mammalian 6-o-phospho-kinases: Substrate specificity and applications","author":"Liu","year":"2024","journal-title":"Biochemistry"},{"issue":"1","key":"10.1016\/bs.mie.2026.05.026_bib21","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1080\/21505594.2021.2019950","article-title":"Pathogenesis and virulence of Candida albicans","volume":"13","author":"Lopes","year":"2022","journal-title":"Virulence"},{"issue":"23","key":"10.1016\/bs.mie.2026.05.026_bib22","doi-asserted-by":"crossref","first-page":"14392","DOI":"10.1074\/jbc.273.23.14392","article-title":"The eukaryotic UDP-N-acetylglucosamine pyrophosphorylases. Gene cloning, protein expression, and catalytic mechanism","volume":"273","author":"Mio","year":"1998","journal-title":"The Journal of Biological Chemistry"},{"issue":"2-3","key":"10.1016\/bs.mie.2026.05.026_bib23","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/S0167-4781(00)00120-2","article-title":"Functional cloning and mutational analysis of the human cDNA for phosphoacetylglucosamine mutase: Identification of the amino acid residues essential for the catalysis","volume":"1492","author":"Mio","year":"2000","journal-title":"Biochimica et Biophysica Acta"},{"key":"10.1016\/bs.mie.2026.05.026_bib24","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/978-981-13-7318-3_2","article-title":"Chitin: Structure, chemistry and biology","volume":"1142","author":"Moussian","year":"2019","journal-title":"Advances in Experimental Medicine and Biology"},{"issue":"8","key":"10.1016\/bs.mie.2026.05.026_bib25","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.1002\/anie.200805507","article-title":"Selective fluorescence labeling of lipids in living cells","volume":"48","author":"Neef","year":"2009","journal-title":"Angewandte Chemie (International Ed. in English)"},{"issue":"Pt 4","key":"10.1016\/bs.mie.2026.05.026_bib26","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1107\/S1744309106010177","article-title":"Purification, crystallization and preliminary X-ray diffraction studies of N-acetylglucosamine-phosphate mutase from Candida albicans","volume":"62","author":"Nishitani","year":"2006","journal-title":"Acta Crystallographica. Section F, Structural Biology and Crystallization Communications"},{"key":"10.1016\/bs.mie.2026.05.026_bib27","unstructured":"Organization,\u00a0W. H.\u00a0(2022). WHO fungal priority pathogens list to guide research, development, and public health action. Retrieved from. https:\/\/www.who.int\/publications\/i\/item\/9789240060241."},{"issue":"1","key":"10.1016\/bs.mie.2026.05.026_bib28","doi-asserted-by":"crossref","first-page":"S79","DOI":"10.1093\/ofid\/ofy358","article-title":"Twenty years of the SENTRY antifungal surveillance program: Results for Candida species from 1997-2016","volume":"6","author":"Pfaller","year":"2019","journal-title":"Open Forum Infectious Diseases"},{"issue":"27","key":"10.1016\/bs.mie.2026.05.026_bib29","doi-asserted-by":"crossref","first-page":"14975","DOI":"10.1002\/anie.202101483","article-title":"General, divergent platform for diastereoselective synthesis of trans-cyclooctenes with high reactivity and favorable physiochemical properties\u204e","volume":"60","author":"Pigga","year":"2021","journal-title":"Angewandte Chemie (International Ed. in English)"},{"issue":"14","key":"10.1016\/bs.mie.2026.05.026_bib30","doi-asserted-by":"crossref","first-page":"2596","DOI":"10.1002\/1521-3773(20020715)41:14<2596::AID-ANIE2596>3.0.CO;2-4","article-title":"A stepwise huisgen cycloaddition process: Copper(I)-catalyzed regioselective \u201cligation\u201d of azides and terminal alkynes","volume":"41","author":"Rostovtsev","year":"2002","journal-title":"Angewandte Chemie (International Ed. in English)"},{"issue":"7","key":"10.1016\/bs.mie.2026.05.026_bib31","doi-asserted-by":"crossref","first-page":"2415","DOI":"10.1073\/pnas.0712168105","article-title":"A chemical method for fast and sensitive detection of DNA synthesis in vivo","volume":"105","author":"Salic","year":"2008","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"5460","key":"10.1016\/bs.mie.2026.05.026_bib32","doi-asserted-by":"crossref","first-page":"2007","DOI":"10.1126\/science.287.5460.2007","article-title":"Cell surface engineering by a modified Staudinger reaction","volume":"287","author":"Saxon","year":"2000","journal-title":"Science (New York, N. Y.)"},{"key":"10.1016\/bs.mie.2026.05.026_bib33","doi-asserted-by":"crossref","DOI":"10.1038\/s43586-021-00028-z","article-title":"Bioorthogonal chemistry","volume":"1","author":"Scinto","year":"2021","journal-title":"Nature Reviews Methods Primers"},{"issue":"38","key":"10.1016\/bs.mie.2026.05.026_bib34","doi-asserted-by":"crossref","first-page":"6974","DOI":"10.1002\/anie.200900942","article-title":"Bioorthogonal chemistry: Fishing for selectivity in a sea of functionality","volume":"48","author":"Sletten","year":"2009","journal-title":"Angewandte Chemie (International Ed. in English)"},{"key":"10.1016\/bs.mie.2026.05.026_bib35","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1002\/hlca.19190020164","article-title":"On new organic phosphorus bonding III Phosphine methylene derivatives and phosphinimine","volume":"2","author":"Staudinger","year":"1919","journal-title":"Helvetica Chimica Acta"},{"issue":"9","key":"10.1016\/bs.mie.2026.05.026_bib36","doi-asserted-by":"crossref","first-page":"3057","DOI":"10.1021\/jo011148j","article-title":"Peptidotriazoles on solid phase: [1,2,3]-triazoles by regiospecific copper(i)-catalyzed 1,3-dipolar cycloadditions of terminal alkynes to azides","volume":"67","author":"Tornoe","year":"2002","journal-title":"The Journal of Organic Chemistry"},{"issue":"4","key":"10.1016\/bs.mie.2026.05.026_bib37","article-title":"Caspofungin-induced beta(1,3)-glucan exposure in Candida albicans is driven by increased chitin levels","volume":"14","author":"Wagner","year":"2023","journal-title":"mBio"},{"issue":"4","key":"10.1016\/bs.mie.2026.05.026_bib38","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000040","article-title":"Stimulation of chitin synthesis rescues Candida albicans from echinocandins","volume":"4","author":"Walker","year":"2008","journal-title":"PLoS Pathogens"},{"key":"10.1016\/bs.mie.2026.05.026_bib39","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1146\/annurev.micro.61.080706.093341","article-title":"Morphogenesis in Candida albicans","volume":"61","author":"Whiteway","year":"2007","journal-title":"Annual Review of Microbiology"},{"issue":"7","key":"10.1016\/bs.mie.2026.05.026_bib40","doi-asserted-by":"crossref","first-page":"5632","DOI":"10.1021\/jacs.4c11554","article-title":"Bio-orthogonal labeling of chitin in native pathogenic candida species via the chitin scavenge pathway","volume":"147","author":"Williams","year":"2025","journal-title":"Journal of the American Chemical Society"}],"container-title":["Methods in Enzymology","Bioorthogonal Labeling"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0076687926001412?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0076687926001412?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T16:52:36Z","timestamp":1782406356000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0076687926001412"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9780443432248"],"references-count":40,"URL":"https:\/\/doi.org\/10.1016\/bs.mie.2026.05.026","relation":{},"ISSN":["0076-6879"],"issn-type":[{"value":"0076-6879","type":"print"}],"subject":[],"published":{"date-parts":[[2026]]}}}