{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:15:34Z","timestamp":1790615734523,"version":"4.1.0"},"reference-count":63,"publisher":"Elsevier","isbn-type":[{"value":"9780443521607","type":"print"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1016\/bs.mie.2026.05.053","type":"book-chapter","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T11:22:04Z","timestamp":1780658524000},"page":"457-490","source":"Crossref","is-referenced-by-count":0,"title":["Manipulating activity and chemoselectivity of a benzaldehyde lyase to synthesize \u03b1-hydroxyketones"],"prefix":"10.1016","author":[{"given":"Yu","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weidong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhui","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiyuan","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaqing","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dunming","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/bs.mie.2026.05.053_bib1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.softx.2015.06.001","article-title":"GROMACS: High performance molecular simulations through multi-level parallelism from laptops to supercomputers","volume":"1-2","author":"Abraham","year":"2015","journal-title":"SoftwareX"},{"issue":"5","key":"10.1016\/bs.mie.2026.05.053_bib2","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1021\/op900141r","article-title":"Large-scale synthesis of a selective inhibitor of the norepinephrine transporter: mechanistic aspects of conversion of indolinone diol to indolinone aminoalcohol and process implications","volume":"13","author":"Alimardanov","year":"2009","journal-title":"Organic Process Research & Development"},{"issue":"39","key":"10.1016\/bs.mie.2026.05.053_bib3","first-page":"13999","article-title":"Regio- and stereoselective aliphatic-aromatic cross-benzoin reaction: Enzymatic divergent catalysis","volume":"22","author":"Beigi","year":"2016","journal-title":"Chemistry (Weinheim an der Bergstrasse, Germany)"},{"issue":"7","key":"10.1016\/bs.mie.2026.05.053_bib4","doi-asserted-by":"crossref","first-page":"4346","DOI":"10.1021\/cr100299p","article-title":"C-C bond-forming lyases in organic synthesis","volume":"111","author":"Brovetto","year":"2011","journal-title":"Chemical Reviews"},{"key":"10.1016\/bs.mie.2026.05.053_bib5","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1107\/S0907444998003254","article-title":"Crystallography & NMR system: A new software suite for macromolecular structure determination","volume":"54","author":"Brunger","year":"1998","journal-title":"Acta Crystallographica Section D, Structural Biology"},{"key":"10.1016\/bs.mie.2026.05.053_bib6","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1126\/science.abh4049","article-title":"Cell-free chemoenzymatic starch synthesis from carbon dioxide","volume":"373","author":"Cai","year":"2021","journal-title":"Science (New York, N. Y.)"},{"issue":"4","key":"10.1016\/bs.mie.2026.05.053_bib7","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1002\/open.201500045","article-title":"Highly efficient synthesis of optically pure (S)-1-phenyl-1,2-ethanediol by a self-sufficient whole cell biocatalyst","volume":"4","author":"Chen","year":"2015","journal-title":"ChemistryOpen"},{"issue":"5","key":"10.1016\/bs.mie.2026.05.053_bib8","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1002\/adsc.200700550","article-title":"\u03b1,\u03b2\u2010Unsaturated aldehydes as substrates for asymmetric C-C bond forming reactions with thiamin diphosphate (ThDP)\u2010dependent enzymes","volume":"350","author":"Cosp","year":"2008","journal-title":"Advanced Synthesis & Catalysis"},{"issue":"1","key":"10.1016\/bs.mie.2026.05.053_bib9","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1002\/adsc.200700230","article-title":"Enantioselective C-C bond ligation using recombinant Escherichia coli\u2010whole\u2010cell biocatalysts","volume":"350","author":"de Mar\u00eda","year":"2008","journal-title":"Advanced Synthesis and Catalysis"},{"issue":"31","key":"10.1016\/bs.mie.2026.05.053_bib10","doi-asserted-by":"crossref","first-page":"6509","DOI":"10.1016\/j.tet.2004.06.015","article-title":"Enzyme catalyzed hydroxymethylation of aromatic aldehydes with formaldehyde. Synthesis of hydroxyacetophenones and (S)-benzoins","volume":"60","author":"Demir","year":"2004","journal-title":"Tetrahedron"},{"issue":"7","key":"10.1016\/bs.mie.2026.05.053_bib11","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1039\/b100341k","article-title":"Enantioselective synthesis of hydroxy ketones through cleavage and formation of acyloin linkage. Enzymatic kinetic resolution via C\u2013C bond cleavage","author":"Demir","year":"2001","journal-title":"Journal of the Chemical Society, Perkin Transactions 1"},{"issue":"12","key":"10.1016\/bs.mie.2026.05.053_bib12","doi-asserted-by":"crossref","first-page":"2047","DOI":"10.1021\/ol034415b","article-title":"Benzaldehyde lyase-catalyzed enantioselective carboligation of aromatic aldehydes with mono- and dimethoxy acetaldehyde","volume":"5","author":"Demir","year":"2003","journal-title":"Organic Letters"},{"issue":"1","key":"10.1016\/bs.mie.2026.05.053_bib13","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1002\/1615-4169(200201)344:1<96::AID-ADSC96>3.0.CO;2-Z","article-title":"Enantioselective synthesis of \u03b1-hydroxy ketones via benzaldehyde lyase-catalyzed C\u2212C bond formation reaction","volume":"344","author":"Demir","year":"2002","journal-title":"Advanced Synthesis & Catalysis"},{"key":"10.1016\/bs.mie.2026.05.053_bib14","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1107\/S0907444904019158","article-title":"Coot: model-building tools for molecular graphics","volume":"60","author":"Emsley","year":"2004","journal-title":"Acta Crystallographica. Section D, Biological Crystallography"},{"issue":"5","key":"10.1016\/bs.mie.2026.05.053_bib15","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1021\/np300204p","article-title":"\u03b1-Glucosidase inhibitors from Brickellia cavanillesii","volume":"75","author":"Escand\u00f3n-Rivera","year":"2012","journal-title":"Journal of Natural Products"},{"issue":"27","key":"10.1016\/bs.mie.2026.05.053_bib16","first-page":"8260","article-title":"Structure-guided engineering of benzaldehyde lyase for efficient synthesis of dihydroxyacetone and one-pot biosynthesis of 2-amino-1,3-propanediol","volume":"27","author":"Fan","year":"2025","journal-title":"Green Chemistry: an International Journal and Green Chemistry Resource: GC"},{"issue":"47","key":"10.1016\/bs.mie.2026.05.053_bib17","doi-asserted-by":"crossref","first-page":"7615","DOI":"10.1002\/ejoc.201900945","article-title":"Building up quaternary stereocenters through biocatalyzed direct insertion of carbon nucleophiles on ketones","volume":"2019","author":"Gaggero","year":"2019","journal-title":"European Journal of Organic Chemistry"},{"issue":"14-15","key":"10.1016\/bs.mie.2026.05.053_bib18","doi-asserted-by":"crossref","first-page":"2805","DOI":"10.1002\/adsc.201200284","article-title":"Influence of organic solvents on enzymatic asymmetric carboligations","volume":"354","author":"Gerhards","year":"2012","journal-title":"Advanced Synthesis & Catalysis"},{"issue":"4","key":"10.1016\/bs.mie.2026.05.053_bib19","doi-asserted-by":"crossref","first-page":"871","DOI":"10.12693\/APhysPolA.121.871","article-title":"Data processing programs for analysis of diffraction images of macromolecular crystals recorded using synchrotron radiation","volume":"121","author":"Gilski","year":"2012","journal-title":"Acta Physica Polonica A"},{"issue":"26","key":"10.1016\/bs.mie.2026.05.053_bib20","doi-asserted-by":"crossref","first-page":"4441","DOI":"10.1002\/ejoc.201600228","article-title":"Thiamine-diphosphate-dependent enzymes as catalytic tools for the asymmetric benzoin-type reaction","volume":"2016","author":"Giovannini","year":"2016","journal-title":"European Journal of Organic Chemistry"},{"issue":"5","key":"10.1016\/bs.mie.2026.05.053_bib21","doi-asserted-by":"crossref","first-page":"2401","DOI":"10.1128\/jb.171.5.2401-2405.1989","article-title":"Benzaldehyde lyase, a novel thiamine PPi-requiring enzyme, from Pseudomonas-Fluorescens Biovar-I","volume":"171","author":"Gonzalez","year":"1989","journal-title":"Journal of Bacteriology"},{"issue":"17","key":"10.1016\/bs.mie.2026.05.053_bib22","first-page":"6583","article-title":"Design of a synthetic enzyme cascade for the in vitro fixation of a C1 carbon source to a functional C4 sugar","volume":"23","author":"G\u00fcner","year":"2021","journal-title":"Green Chemistry: an International Journal and Green Chemistry Resource: GC"},{"issue":"2","key":"10.1016\/bs.mie.2026.05.053_bib23","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1111\/1751-7915.13602","article-title":"Green and scalable synthesis of chiral aromatic alcohols through an efficient biocatalytic system","volume":"14","author":"Han","year":"2020","journal-title":"Microbial Biotechnology"},{"issue":"5","key":"10.1016\/bs.mie.2026.05.053_bib24","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1002\/bit.21189","article-title":"The production of (R)-2-hydroxy-1-phenyl-propan-1-one derivatives by benzaldehyde lyase from Pseudomonas fluorescens in a continuously operated membrane reactor","volume":"96","author":"Hildebrand","year":"2007","journal-title":"Biotechnology and Bioengineering"},{"issue":"1","key":"10.1016\/bs.mie.2026.05.053_bib25","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0263-7855(96)00018-5","article-title":"VMD: Visual molecular dynamics","volume":"14","author":"Humphrey","year":"1996","journal-title":"Journal of Molecular Graphics and Modelling"},{"issue":"35","key":"10.1016\/bs.mie.2026.05.053_bib26","doi-asserted-by":"crossref","first-page":"11093","DOI":"10.1021\/jacs.6b06156","article-title":"An Ir\/Zn Dual catalysis for enantio- and diastereodivergent \u03b1-allylation of \u03b1-hydroxyketones","volume":"138","author":"Huo","year":"2016","journal-title":"Journal of the American Chemical Society"},{"issue":"7","key":"10.1016\/bs.mie.2026.05.053_bib27","first-page":"3472","article-title":"A Two-step biocatalytic cascade in micro-aqueous medium: Using whole cells to obtain high concentrations of a vicinal diol","volume":"16","author":"Jakoblinnert","year":"2014","journal-title":"Green Chemistry: an International Journal and Green Chemistry Resource: GC"},{"issue":"6","key":"10.1016\/bs.mie.2026.05.053_bib28","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.bioorg.2006.09.002","article-title":"Characterization of benzaldehyde lyase from Pseudomonas fluorescens: A versatile enzyme for asymmetric C-C bond formation","volume":"34","author":"Janzen","year":"2006","journal-title":"Bioorganic Chemistry"},{"issue":"D1","key":"10.1016\/bs.mie.2026.05.053_bib29","doi-asserted-by":"crossref","first-page":"D1670","DOI":"10.1093\/nar\/gkae973","article-title":"Generic diagramming platform (GDP): A comprehensive database of high-quality biomedical graphics","volume":"53","author":"Jiang","year":"2025","journal-title":"Nucleic Acids Research"},{"key":"10.1016\/bs.mie.2026.05.053_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcat.2024.113956","article-title":"Enantioselective synthesis of vicinal diols by an enzymatic approach","volume":"557","author":"Ju","year":"2024","journal-title":"Molecular Catalysis"},{"issue":"20","key":"10.1016\/bs.mie.2026.05.053_bib31","doi-asserted-by":"crossref","first-page":"14803","DOI":"10.1021\/acs.joc.3c01641","article-title":"A Chemo-enzymatic approach for preparing Efinaconazole with high optical yield","volume":"88","author":"Ju","year":"2023","journal-title":"The Journal of Organic Chemistry"},{"issue":"7873","key":"10.1016\/bs.mie.2026.05.053_bib32","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"issue":"1","key":"10.1016\/bs.mie.2026.05.053_bib33","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1038\/s42004-019-0224-2","article-title":"How formaldehyde reacts with amino acids","volume":"2","author":"Kamps","year":"2019","journal-title":"Communications Chemistry"},{"issue":"14","key":"10.1016\/bs.mie.2026.05.053_bib34","doi-asserted-by":"crossref","first-page":"6095","DOI":"10.1007\/s00253-018-9041-2","article-title":"Boosting the efficiency of site-saturation mutagenesis for a difficult-to-randomize gene by a two-step PCR strategy","volume":"102","author":"Li","year":"2018","journal-title":"Applied Microbiology and Biotechnology"},{"issue":"4","key":"10.1016\/bs.mie.2026.05.053_bib35","doi-asserted-by":"crossref","first-page":"3102","DOI":"10.1021\/jacs.4c11957","article-title":"Taming highly enolizable aldehydes via enzyme catalysis for enantiocomplementary construction of \u03b2-hydroxyphosphonates","volume":"147","author":"Li","year":"2025","journal-title":"Journal of the American Chemical Society"},{"issue":"17","key":"10.1016\/bs.mie.2026.05.053_bib36","doi-asserted-by":"crossref","DOI":"10.1002\/anie.202116344","article-title":"Asymmetric synthesis of N-substituted 1,2-amino alcohols from simple aldehydes and amines by one-pot sequential enzymatic hydroxymethylation and asymmetric reductive amination","volume":"61","author":"Li","year":"2022","journal-title":"Angewandte Chemie International Edition"},{"issue":"1","key":"10.1016\/bs.mie.2026.05.053_bib37","doi-asserted-by":"crossref","DOI":"10.1016\/j.checat.2022.11.006","article-title":"Creating a new benzaldehyde lyase for atom-economic synthesis of chiral 1,2,4-butanetriol and 2-aminobutane-1,4-diol from formaldehyde","volume":"3","author":"Li","year":"2023","journal-title":"Chem Catalysis"},{"key":"10.1016\/bs.mie.2026.05.053_bib38","unstructured":"Lu, T. Sobtop Version 1.0 (dev3.1). http:\/\/sobereva.com\/soft\/Sobtop (accessed on 17-Aug-2022)."},{"issue":"5","key":"10.1016\/bs.mie.2026.05.053_bib39","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1002\/jcc.22885","article-title":"Multiwfn: A multifunctional wavefunction analyzer","volume":"33","author":"Lu","year":"2011","journal-title":"Journal of Computational Chemistry"},{"key":"10.1016\/bs.mie.2026.05.053_bib40","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.molcatb.2014.06.003","article-title":"Benzaldehyde lyase (BAL)-catalyzed enantioselective C-C bond formation in deep-eutectic-solvents\u2013buffer mixtures","volume":"107","author":"Maugeri","year":"2014","journal-title":"Journal of Molecular Catalysis B: Enzymatic"},{"key":"10.1016\/bs.mie.2026.05.053_bib41","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","article-title":"Phaser crystallographic software","volume":"40","author":"Mccoy","year":"2007","journal-title":"Journal of Applied Crystallography"},{"issue":"12","key":"10.1016\/bs.mie.2026.05.053_bib42","doi-asserted-by":"crossref","first-page":"2000","DOI":"10.1039\/c2ob27344f","article-title":"Benzaldehyde lyase-catalyzed diastereoselective C-C bond formation by simultaneous carboligation and kinetic resolution","volume":"11","author":"M\u00fcller","year":"2013","journal-title":"Organic & Biomolecular Chemistry"},{"issue":"2","key":"10.1016\/bs.mie.2026.05.053_bib43","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.cbpa.2013.02.017","article-title":"C-C Bond formation using ThDP-dependent lyases","volume":"17","author":"M\u00fcller","year":"2013","journal-title":"Current Opinion in Chemical Biology"},{"key":"10.1016\/bs.mie.2026.05.053_bib44","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1107\/S0907444911001314","article-title":"REFMAC5 for the refinement of macromolecular crystal structures","volume":"67","author":"Murshudov","year":"2011","journal-title":"Acta Crystallographica. Section D, Biological Crystallography"},{"issue":"33","key":"10.1016\/bs.mie.2026.05.053_bib45","doi-asserted-by":"crossref","first-page":"6245","DOI":"10.1016\/S0040-4039(03)01554-5","article-title":"Enantioselective synthesis of (R)-phenylephrine hydrochloride","volume":"44","author":"Pandey","year":"2003","journal-title":"Tetrahedron Letters"},{"issue":"13","key":"10.1016\/bs.mie.2026.05.053_bib46","doi-asserted-by":"crossref","first-page":"1950","DOI":"10.1002\/cbic.201500228","article-title":"Mechanistic analysis of an engineered enzyme that catalyzes the formose reaction","volume":"16","author":"Poust","year":"2015","journal-title":"Chembiochem: a European Journal of Chemical Biology"},{"issue":"4","key":"10.1016\/bs.mie.2026.05.053_bib47","doi-asserted-by":"crossref","first-page":"1976","DOI":"10.1021\/jm501921k","article-title":"Discovery of highly potent, selective, and efficacious small molecule inhibitors of ERK1\/2","volume":"58","author":"Ren","year":"2015","journal-title":"Journal of Medicinal Chemistry"},{"issue":"12","key":"10.1016\/bs.mie.2026.05.053_bib48","doi-asserted-by":"crossref","first-page":"3796","DOI":"10.1002\/bit.28841","article-title":"Rational design of short-chain dehydrogenase\/reductase for enantio-complementary synthesis of chiral 1,2-diols by successive hydroxymethylation and reduction of aldehydes","volume":"121","author":"Ren","year":"2024","journal-title":"Biotechnology and Bioengineering"},{"issue":"17","key":"10.1016\/bs.mie.2026.05.053_bib49","doi-asserted-by":"crossref","first-page":"15395","DOI":"10.1021\/acscatal.5c03333","article-title":"Network dynamics as fingerprints of thermostability in an in silico-engineered DyP-type peroxidase","volume":"15","author":"Rodrigues","year":"2025","journal-title":"ACS Catalysis"},{"issue":"6-7","key":"10.1016\/bs.mie.2026.05.053_bib50","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1002\/adsc.200303033","article-title":"Mixed aromatic acyloin condensations with recombinant benzaldehyde lyase: Synthesis of \u03b1\u2010hydroxydihydrochalcones and related \u03b1\u2010hydroxy ketones","volume":"345","author":"Sanchez\u2010Gonzalez","year":"2003","journal-title":"Advanced Synthesis & Catalysis"},{"issue":"1","key":"10.1016\/bs.mie.2026.05.053_bib51","first-page":"94","article-title":"Oxidation-hydroxymethylation-reduction: A one-pot three-step biocatalytic synthesis of optically active \u03b1-aryl vicinal diols","volume":"14","author":"Shanmuganathan","year":"2012","journal-title":"Green Chemistry: an International Journal and Green Chemistry Resource: GC"},{"issue":"12","key":"10.1016\/bs.mie.2026.05.053_bib52","doi-asserted-by":"crossref","first-page":"3704","DOI":"10.1073\/pnas.1500545112","article-title":"Computational protein design enables a novel one-carbon assimilation pathway","volume":"112","author":"Siegel","year":"2015","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"10","key":"10.1016\/bs.mie.2026.05.053_bib53","article-title":"Manganese octahedral molecular sieves catalyzed tandem process for synthesis of quinoxalines","volume":"10","author":"Sithambaram","year":"2008","journal-title":"Green Chemistry: an International Journal and Green Chemistry Resource: GC"},{"issue":"20","key":"10.1016\/bs.mie.2026.05.053_bib54","doi-asserted-by":"crossref","first-page":"7062","DOI":"10.1016\/j.bmc.2006.06.008","article-title":"A Colorimetric assay for screening transketolase activity","volume":"14","author":"Smith","year":"2006","journal-title":"Bioorganic & Medicinal Chemistry"},{"key":"10.1016\/bs.mie.2026.05.053_bib55","doi-asserted-by":"crossref","DOI":"10.1016\/j.gresc.2025.08.003","article-title":"Asymmetric synthesis of structurally diverse (S)-\u03b2-amino alcohols from simple aldehydes and formaldehyde","author":"Song","year":"2025","journal-title":"Green Synthesis and Catalysis"},{"issue":"10","key":"10.1016\/bs.mie.2026.05.053_bib56","doi-asserted-by":"crossref","first-page":"6281","DOI":"10.1021\/acs.jctc.1c00645","article-title":"gmx_MMPBSA: A new tool to perform end-state free energy calculations with GROMACS","volume":"17","author":"Vald\u00e9s-Tresanco","year":"2021","journal-title":"Journal of Chemical Theory and Computation"},{"issue":"9","key":"10.1016\/bs.mie.2026.05.053_bib57","doi-asserted-by":"crossref","first-page":"4584","DOI":"10.1021\/acs.jctc.7b00500","article-title":"POVME 3.0: Software for mapping binding pocket flexibility","volume":"13","author":"Wagner","year":"2017","journal-title":"Journal of Chemical Theory and Computation"},{"issue":"6","key":"10.1016\/bs.mie.2026.05.053_bib58","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1016\/S0960-894X(02)01058-2","article-title":"Inhibitors of A\u03b2 production: Solid-phase synthesis and SAR of \u03b1-hydroxycarbonyl derivatives","volume":"13","author":"Wallace","year":"2003","journal-title":"Bioorganic & Medicinal Chemistry Letters"},{"issue":"4","key":"10.1016\/bs.mie.2026.05.053_bib59","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1039\/C6QO00810K","article-title":"Asymmetric hydrogenation of \u03b1-hydroxy ketones with an iridium\/f-amphox catalyst: Efficient access to chiral 1, 2-diols","volume":"4","author":"Wu","year":"2017","journal-title":"Organic Chemistry Frontiers"},{"key":"10.1016\/bs.mie.2026.05.053_bib60","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2025.159573","article-title":"Innovative coupling pathway for second- and third-generation biomass: Efficient l-lactate synthesis from xylose and C1 compounds","volume":"505","author":"Wu","year":"2025","journal-title":"Chemical Engineering Journal"},{"key":"10.1016\/bs.mie.2026.05.053_bib61","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2022.107926","article-title":"Hot spots-making directed evolution easier","volume":"56","author":"Yu","year":"2022","journal-title":"Biotechnology Advances"},{"issue":"13","key":"10.1016\/bs.mie.2026.05.053_bib62","doi-asserted-by":"crossref","first-page":"9687","DOI":"10.1021\/acscatal.4c01804","article-title":"Manipulating activity and chemoselectivity of a benzaldehyde lyase for efficient synthesis of \u03b1-hydroxymethyl ketones and one-pot enantio-complementary conversion to 1,2-diols","volume":"14","author":"Zhang","year":"2024","journal-title":"ACS Catalysis"},{"issue":"7","key":"10.1016\/bs.mie.2026.05.053_bib63","doi-asserted-by":"crossref","first-page":"4768","DOI":"10.1021\/acscatal.2c06417","article-title":"Flipping the substrate creates a highly selective halohydrin dehalogenase for the synthesis of chiral 4-aryl-2-oxazolidinones from readily available epoxides","volume":"13","author":"Zhou","year":"2023","journal-title":"ACS Catalysis"}],"container-title":["Methods in Enzymology","Thiamine Pyrophosphate (TPP)-Dependent Enzymes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0076687926001813?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0076687926001813?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:28:17Z","timestamp":1790612897000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0076687926001813"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9780443521607"],"references-count":63,"URL":"https:\/\/doi.org\/10.1016\/bs.mie.2026.05.053","relation":{},"ISSN":["0076-6879"],"issn-type":[{"value":"0076-6879","type":"print"}],"subject":[],"published":{"date-parts":[[2026]]}}}