{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T05:55:10Z","timestamp":1791438910022,"version":"4.3.3"},"reference-count":32,"publisher":"Elsevier","isbn-type":[{"value":"9780443521607","type":"print"}],"license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100009053","name":"Wellcome Trust DBT India Alliance","doi-asserted-by":"publisher","award":["IA\/S\/19\/1\/504263"],"award-info":[{"award-number":["IA\/S\/19\/1\/504263"]}],"id":[{"id":"10.13039\/501100009053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["IIRP-2731-2023"],"award-info":[{"award-number":["IIRP-2731-2023"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["EMDR\/FIW\/10\/2025-00000070"],"award-info":[{"award-number":["EMDR\/FIW\/10\/2025-00000070"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1016\/bs.mie.2026.07.016","type":"book-chapter","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T11:36:33Z","timestamp":1786707393000},"page":"573-598","source":"Crossref","is-referenced-by-count":0,"title":["Thiamine pyrophosphate-dependent enzymes in Toxoplasma gondii and functional significance of an apicoplast-localized pyruvate dehydrogenase"],"prefix":"10.1016","author":[{"given":"Sushanta","family":"Chhatar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ketan","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bang","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nishith","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"31","key":"10.1016\/bs.mie.2026.07.016_bib1","doi-asserted-by":"crossref","first-page":"12998","DOI":"10.1073\/pnas.0903831106","article-title":"Host-derived glucose and its transporter in the obligate intracellular pathogen Toxoplasma gondii are dispensable by glutaminolysis","volume":"106","author":"Blume","year":"2009","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"10.1016\/bs.mie.2026.07.016_bib2","doi-asserted-by":"crossref","DOI":"10.12688\/f1000research.16021.1","article-title":"Metabolic interactions between Toxoplasma gondii and its host","volume":"7","author":"Blume","year":"2018","journal-title":"F1000Research"},{"issue":"4","key":"10.1016\/bs.mie.2026.07.016_bib3","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01380-21","article-title":"The dissection of SNAREs reveals key factors for vesicular trafficking to the endosome-like compartment and apicoplast via the secretory system in Toxoplasma gondii","volume":"12","author":"Cao","year":"2021","journal-title":"mBio"},{"key":"10.1016\/bs.mie.2026.07.016_bib4","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1136\/jcp.2009.072066","article-title":"Toxoplasma tachyzoites from cell culture are more appropriate in some situations","volume":"63","author":"Chatterton","year":"2010","journal-title":"Journal of Clinical Pathology"},{"issue":"25","key":"10.1016\/bs.mie.2026.07.016_bib5","article-title":"A pyruvate transporter in the apicoplast of apicomplexan parasites","volume":"121","author":"Chen","year":"2024","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"6","key":"10.1016\/bs.mie.2026.07.016_bib6","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.pt.2017.02.007","article-title":"The Toxoplasma Parasitophorous vacuole, an evolving host\u2013parasite frontier","volume":"33","author":"Clough","year":"2017","journal-title":"Trends in Parasitology"},{"key":"10.1016\/bs.mie.2026.07.016_bib7","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1007\/s00239-007-9056-2","article-title":"Evolutionary analysis of the TPP-dependent enzyme family","volume":"66","author":"Costelloe","year":"2008","journal-title":"Journal of Molecular Evolution"},{"key":"10.1016\/bs.mie.2026.07.016_bib8","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1128\/EC.00061-07","article-title":"Carbohydrate metabolism in the Toxoplasma gondii apicoplast: Localization of three glycolytic isoenzymes, the single pyruvate dehydrogenase complex, and a plastid phosphate translocator","volume":"6","author":"Fleige","year":"2007","journal-title":"Eukaryotic Cell"},{"key":"10.1016\/bs.mie.2026.07.016_bib9","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1111\/j.1365-2958.2004.04407.x","article-title":"The malaria parasite Plasmodium falciparum has only one pyruvate dehydrogenase complex, which is located in the apicoplast","volume":"55","author":"Foth","year":"2005","journal-title":"Molecular Microbiology"},{"issue":"1","key":"10.1016\/bs.mie.2026.07.016_bib10","doi-asserted-by":"crossref","first-page":"6642","DOI":"10.1038\/s41467-024-50671-9","article-title":"Cryo-EM structure of 1-deoxy-D-xylulose 5-phosphate synthase DXPS from Plasmodium falciparum reveals a distinct N-terminal domain","volume":"15","author":"Gawriljuk","year":"2024","journal-title":"Nature Communications"},{"key":"10.1016\/bs.mie.2026.07.016_bib11","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.chom.2015.04.003","article-title":"The Toxoplasma dense granule proteins GRA17 and GRA23 mediate the movement of small molecules between the host and the parasitophorous vacuole","volume":"17","author":"Gold","year":"2015","journal-title":"Cell Host & Microbe"},{"issue":"5","key":"10.1016\/bs.mie.2026.07.016_bib12","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1007\/s00284-026-04823-3","article-title":"The potential of MIC17A both as an entero-epithelial and chronic stage marker for detection of feline Toxoplasmosis","volume":"83","author":"G\u00fcnay-Esiyok","year":"2026","journal-title":"Current Microbiology"},{"issue":"1","key":"10.1016\/bs.mie.2026.07.016_bib13","doi-asserted-by":"crossref","first-page":"2999","DOI":"10.1038\/s41467-024-47097-8","article-title":"Metabolic plasticity, essentiality and therapeutic potential of ribose-5-phosphate synthesis in Toxoplasma gondii","volume":"15","author":"Guo","year":"2024","journal-title":"Nature Communications"},{"key":"10.1016\/bs.mie.2026.07.016_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.rvsc.2023.105054","article-title":"An Inhouse ELISA using recombinant GRA1 and BAG1 proteins as antigen successfully detected Toxoplasma gondii infection in a flock of sheep suffering from abortions","volume":"165","author":"Kandemir","year":"2023","journal-title":"Research in Veterinary Science"},{"issue":"3","key":"10.1016\/bs.mie.2026.07.016_bib15","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.ijpara.2003.12.009","article-title":"Toxoplasma gondii: the model apicomplexan","volume":"34","author":"Kim","year":"2004","journal-title":"International Journal for Parasitology"},{"key":"10.1016\/bs.mie.2026.07.016_bib16","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1016\/S0021-9258(17)49928-5","article-title":"Vitamin and cofactor acquisition in apicomplexans: Synthesis versus salvage","volume":"295","author":"Krishnan","year":"2020","journal-title":"Journal of Biological Chemistry"},{"key":"10.1016\/bs.mie.2026.07.016_bib17","doi-asserted-by":"crossref","first-page":"7743","DOI":"10.1074\/jbc.RA120.013004","article-title":"Acquisition of exogenous fatty acids renders apicoplast-based biosynthesis dispensable in tachyzoites of Toxoplasma","volume":"295","author":"Liang","year":"2020","journal-title":"Journal of Biological Chemistry"},{"issue":"2","key":"10.1016\/bs.mie.2026.07.016_bib18","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.05043-22","article-title":"The mitochondrial pyruvate carrier coupling glycolysis and the tricarboxylic acid cycle is required for the asexual reproduction of Toxoplasma gondii","volume":"11","author":"Lyu","year":"2023","journal-title":"Microbiology Spectrum"},{"issue":"35","key":"10.1016\/bs.mie.2026.07.016_bib19","doi-asserted-by":"crossref","first-page":"13192","DOI":"10.1073\/pnas.0603391103","article-title":"Apicoplast fatty acid synthesis is essential for organelle biogenesis and parasite survival in Toxoplasma gondii","volume":"103","author":"Mazumdar","year":"2006","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"10.1016\/bs.mie.2026.07.016_bib20","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1093\/bioinformatics\/bts127","article-title":"IsoCor: correcting MS data in isotope labeling experiments","volume":"28","author":"Millard","year":"2012","journal-title":"Bioinformatics"},{"issue":"7","key":"10.1016\/bs.mie.2026.07.016_bib786","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1084\/jem.20110039","article-title":"Apicoplast isoprenoid precursor synthesis and the molecular basis of fosmidomycin resistance in Toxoplasma gondii","volume":"208","author":"Nair","year":"2011","journal-title":"Journal of Experimental Medicine"},{"key":"10.1016\/bs.mie.2026.07.016_bib21","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1074\/jbc.M114.624619","article-title":"Metabolic cooperation of glucose and glutamine is essential for the lytic cycle of obligate intracellular parasite Toxoplasma gondii","volume":"291","author":"Nitzsche","year":"2016","journal-title":"Journal of Biological Chemistry"},{"issue":"7","key":"10.1016\/bs.mie.2026.07.016_bib22","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1004263","article-title":"BCKDH: The missing link in apicomplexan mitochondrial metabolism is required for full virulence of Toxoplasma gondii and Plasmodium berghei","volume":"10","author":"Oppenheim","year":"2014","journal-title":"PLoS Pathogens"},{"issue":"4","key":"10.1016\/bs.mie.2026.07.016_bib23","doi-asserted-by":"crossref","first-page":"270","DOI":"10.4093\/dmj.2018.0101","article-title":"Role of the pyruvate dehydrogenase complex in metabolic remodeling: Differential pyruvate dehydrogenase complex functions in metabolism","volume":"42","author":"Park","year":"2018","journal-title":"Diabetes & Metabolism Journal"},{"issue":"24","key":"10.1016\/bs.mie.2026.07.016_bib24","doi-asserted-by":"crossref","first-page":"16615","DOI":"10.1074\/jbc.R114.563148","article-title":"The pyruvate dehydrogenase complexes: Structure-based function and regulation","volume":"289","author":"Patel","year":"2014","journal-title":"Journal of Biological Chemistry"},{"key":"10.1016\/bs.mie.2026.07.016_bib25","series-title":"Methods in Molecular Biology","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/978-1-4939-6472-7_6","article-title":"Development of CRISPR\/Cas9 for efficient genome editing in Toxoplasma gondii","author":"Shen","year":"2017"},{"issue":"3","key":"10.1016\/bs.mie.2026.07.016_bib26","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01114-14","article-title":"Efficient gene disruption in diverse strains of Toxoplasma gondii using CRISPR\/Cas9","volume":"5","author":"Shen","year":"2014","journal-title":"mBio"},{"issue":"5","key":"10.1016\/bs.mie.2026.07.016_bib27","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1004261","article-title":"Metabolic needs and capabilities of Toxoplasma gondii through combined computational and experimental analysis","volume":"11","author":"Tymoshenko","year":"2015","journal-title":"PLoS Computational Biology"},{"issue":"6","key":"10.1016\/bs.mie.2026.07.016_bib28","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.02021-14","article-title":"Genetic manipulation of the Toxoplasma gondii genome by fosmid recombineering","volume":"5","author":"Vinayak","year":"2014","journal-title":"mBio"},{"key":"10.1016\/bs.mie.2026.07.016_bib29","series-title":"Methods in enzymology","first-page":"97","article-title":"Function and therapeutic potential of transketolase enzymes in parasitic protists","author":"Xia","year":"2025"},{"issue":"3","key":"10.1016\/bs.mie.2026.07.016_bib30","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00898-19","article-title":"Pyruvate homeostasis as a determinant of parasite growth and metabolic plasticity in Toxoplasma gondii","volume":"10","author":"Xia","year":"2019","journal-title":"mBio"},{"issue":"26","key":"10.1016\/bs.mie.2026.07.016_bib31","doi-asserted-by":"crossref","first-page":"14802","DOI":"10.1073\/pnas.011597698","article-title":"The remarkable structural and functional organization of the eukaryotic pyruvate dehydrogenase complexes","volume":"98","author":"Zhou","year":"2001","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"}],"container-title":["Methods in Enzymology","Thiamine Pyrophosphate (TPP)-Dependent Enzymes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0076687926002582?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0076687926002582?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T05:39:38Z","timestamp":1791437978000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0076687926002582"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"ISBN":["9780443521607"],"references-count":32,"URL":"https:\/\/doi.org\/10.1016\/bs.mie.2026.07.016","relation":{},"ISSN":["0076-6879"],"issn-type":[{"value":"0076-6879","type":"print"}],"subject":[],"published":{"date-parts":[[2026]]}}}