{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T15:11:31Z","timestamp":1788102691637,"version":"build-2803163510"},"reference-count":10,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2003,11,1]],"date-time":"2003-11-01T00:00:00Z","timestamp":1067644800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2003,11]]},"DOI":"10.1016\/j.ab.2003.08.012","type":"journal-article","created":{"date-parts":[[2003,10,11]],"date-time":"2003-10-11T05:52:40Z","timestamp":1065851560000},"page":"179-184","source":"Crossref","is-referenced-by-count":20,"title":["Microscale synthesis of 2-tritiated isopropanol and 4R-tritiated reduced nicotinamide adenine dinucleotide phosphate"],"prefix":"10.1016","volume":"322","author":[{"given":"Nitish","family":"Agrawal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amnon","family":"Kohen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2003.08.012_BIB1","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1039\/p19930001213","article-title":"Preparation of [4R-3H] NADH, [4R-3H] NADPH and the corresponding 4S-isomers all with substantial specific activities","volume":"1","author":"Ichinose","year":"1993","journal-title":"J. Chem. Soc. Perkin Trans."},{"key":"10.1016\/j.ab.2003.08.012_BIB2","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1006\/abio.1994.1411","article-title":"A method of preparation and purification of (4R)-deuterated-reduced nicotinamide adenine dinucleotide phosphate","volume":"221","author":"Jeong","year":"1994","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2003.08.012_BIB3","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1111\/j.1399-3011.1993.tb00159.x","article-title":"Stereospecificity of hydrogen transfer by the NADP-linked alcohol dehydrogenase from the thermophilic bacterium Thermoanaerobium brockii","volume":"42","author":"Peretz","year":"1993","journal-title":"Int. J. Peptide Res."},{"key":"10.1016\/j.ab.2003.08.012_BIB4","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1021\/ar9701225","article-title":"Enzyme catalysis: beyond classical paradigms","volume":"31","author":"Kohen","year":"1998","journal-title":"Acc. Chem. Res."},{"key":"10.1016\/j.ab.2003.08.012_BIB5","doi-asserted-by":"crossref","first-page":"R191","DOI":"10.1016\/S1074-5521(99)80058-1","article-title":"Hydrogen tunneling in biology","volume":"6","author":"Kohen","year":"1999","journal-title":"Chem. Biol."},{"key":"10.1016\/j.ab.2003.08.012_BIB6","doi-asserted-by":"crossref","first-page":"3858","DOI":"10.1021\/ja016909e","article-title":"Boundary conditions for the Swain-Schaad relationship as a criterion for hydrogen tunneling","volume":"124","author":"Kohen","year":"2002","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.ab.2003.08.012_BIB7","doi-asserted-by":"crossref","first-page":"4085","DOI":"10.1021\/bi00387a052","article-title":"Construction and evaluation of the kinetic scheme associated with dihydrofolate reductase from Escherichia coli","volume":"26","author":"Frieke","year":"1987","journal-title":"Biochemistry"},{"key":"10.1016\/j.ab.2003.08.012_BIB8","doi-asserted-by":"crossref","first-page":"12618","DOI":"10.1021\/bi026369d","article-title":"Coupling interactions of distal residues enhance dihydrofolate reductase catalysis: mutational effects on hydride transfer rates","volume":"41","author":"Rajagopalan","year":"2002","journal-title":"Biochemistry"},{"key":"10.1016\/j.ab.2003.08.012_BIB9","doi-asserted-by":"crossref","unstructured":"N. Agrawal, A. Kohen, Microscale Synthesis of isotopically labeled R-[6-xH]-N5,N10-methylene-5,6,7,8-tetrahydrofolate as a substrate for thymidylate synthase (2003), submitted","DOI":"10.1016\/j.ab.2004.01.029"},{"key":"10.1016\/j.ab.2003.08.012_BIB10","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.ab.2003.07.010","article-title":"Purification, analysis, and preservation of reduced nicotinamide adenine dinucleotide 2\u2032-phosphate","volume":"322","author":"Markham","year":"2003","journal-title":"Anal. Biochem."}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269703005414?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269703005414?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,3,26]],"date-time":"2020-03-26T00:48:59Z","timestamp":1585183739000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269703005414"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,11]]},"references-count":10,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,11]]}},"alternative-id":["S0003269703005414"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2003.08.012","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2003,11]]}}}