{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T18:05:09Z","timestamp":1758045909035,"version":"3.44.0"},"reference-count":35,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2005,11,1]],"date-time":"2005-11-01T00:00:00Z","timestamp":1130803200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2005,11,1]],"date-time":"2005-11-01T00:00:00Z","timestamp":1130803200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2005,11]]},"DOI":"10.1016\/j.ab.2005.08.003","type":"journal-article","created":{"date-parts":[[2005,8,26]],"date-time":"2005-08-26T07:22:41Z","timestamp":1125040961000},"page":"311-319","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"title":["Study of primary amines for nucleophilic cleavage of cyanylated cystinyl proteins in disulfide mass mapping methodology"],"prefix":"10.1016","volume":"346","author":[{"given":"Jos\u00e9-Luis","family":"Gallegos-P\u00e9rez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Rangel-Ord\u00f3\u00f1ez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen Robert","family":"Bowman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles O.","family":"Ngowe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Throck","family":"Watson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2005.08.003_bib1","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1002\/1615-9861(200209)2:9<1090::AID-PROT1090>3.0.CO;2-1","article-title":"Disulfide proteome in the analysis of protein function and structure","volume":"2","author":"Yano","year":"2002","journal-title":"Proteomics"},{"key":"10.1016\/j.ab.2005.08.003_bib2","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1002\/mas.10025","article-title":"Protein disulfide bond determination by mass spectrometry","volume":"21","author":"Gorman","year":"2002","journal-title":"Mass Spectrom. Rev."},{"key":"10.1016\/j.ab.2005.08.003_bib3","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1002\/elps.200305677","article-title":"Defining the plant disulfide proteome","volume":"25","author":"Lee","year":"2004","journal-title":"Electrophoresis"},{"key":"10.1016\/j.ab.2005.08.003_bib4","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1016\/S0021-9258(19)67918-4","article-title":"The disulfide bonds of ribonuclease","volume":"235","author":"Spackman","year":"1960","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ab.2005.08.003_bib5","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.1126\/science.129.3359.1340","article-title":"Chemistry of insulin; determination of the structure of insulin opens the way to greater understanding of life processes","volume":"129","author":"Sanger","year":"1959","journal-title":"Science"},{"key":"10.1016\/j.ab.2005.08.003_bib6","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1042\/bj0600541","article-title":"The disulphide bonds of insulin","volume":"60","author":"Ryle","year":"1955","journal-title":"Biochem. J."},{"key":"10.1016\/j.ab.2005.08.003_bib7","first-page":"1","article-title":"Assignment of disulfide bonds in proteins by chemical cleavage and peptide mapping by mass spectrometry","volume":"194","author":"Wu","year":"2002","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.ab.2005.08.003_bib8","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1002\/pro.5560060215","article-title":"A novel methodology for assignment of disulfide bond pairings in proteins","volume":"6","author":"Wu","year":"1997","journal-title":"Protein Sci."},{"key":"10.1016\/j.ab.2005.08.003_bib9","first-page":"49","article-title":"Enzymatic cleavage of proteins","volume":"211","author":"Smith","year":"2003","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.ab.2005.08.003_bib10","first-page":"63","article-title":"Chemical cleavage of polypeptides","volume":"211","author":"Smith","year":"2003","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.ab.2005.08.003_bib11","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.ab.2004.10.001","article-title":"Optimizing the connectivity in disulfide-rich peptides: alpha-conotoxin SII as a case study","volume":"338","author":"Bingham","year":"2005","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2005.08.003_bib12","doi-asserted-by":"crossref","first-page":"1732","DOI":"10.1002\/pro.5560021017","article-title":"Disulfide structures of highly bridged peptides: a new strategy for analysis","volume":"2","author":"Gray","year":"1993","journal-title":"Protein Sci."},{"key":"10.1016\/j.ab.2005.08.003_bib13","first-page":"250","article-title":"Disulfide mapping of proteins by mass spectrometry","volume":"Vol. 2","author":"Qi","year":"2004"},{"key":"10.1016\/j.ab.2005.08.003_bib14","doi-asserted-by":"crossref","first-page":"6583","DOI":"10.1016\/S0021-9258(19)43393-0","article-title":"Specific chemical cleavage in high yield at the amino peptide bonds of cysteine and cystine residues","volume":"248","author":"Jacobson","year":"1973","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ab.2005.08.003_bib15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1021\/bi00698a001","article-title":"Cyanylation of sulfhydryl groups by 2-nitro-5-thiocyanobenzoic acid. High-yield modification and cleavage of peptides at cysteine residues","volume":"13","author":"Degani","year":"1974","journal-title":"Biochemistry"},{"key":"10.1016\/j.ab.2005.08.003_bib16","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/0076-6879(77)47015-0","article-title":"Cleavage at cysteine after cyanylation","volume":"47","author":"Stark","year":"1977","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.ab.2005.08.003_bib17","first-page":"5","article-title":"Production of hPTH(1-34) via a recombinant DNA technique","volume":"31","author":"Nakagawa","year":"1993","journal-title":"Peptide Chem."},{"key":"10.1016\/j.ab.2005.08.003_bib18","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.1006\/bbrc.1994.1653","article-title":"Production of human PTH(1-34) via a recombinant DNA technique","volume":"200","author":"Nakagawa","year":"1994","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.ab.2005.08.003_bib19","doi-asserted-by":"crossref","first-page":"5513","DOI":"10.1021\/ja00091a083","article-title":"Chemical cleavage of recombinant fusion proteins to yield peptide amides","volume":"116","author":"Nakagawa","year":"1994","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.ab.2005.08.003_bib20","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1006\/abio.1998.2596","article-title":"Optimization of the cleavage reaction for cyanylated cysteinyl proteins for efficient and simplified mass mapping","volume":"258","author":"Wu","year":"1998","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2005.08.003_bib21","doi-asserted-by":"crossref","first-page":"48188","DOI":"10.1074\/jbc.M308771200","article-title":"Disulfide mapping of the cyclotide kalata B1. Chemical proof of the cystic cystine knot motif","volume":"278","author":"Goransson","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ab.2005.08.003_bib22","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0003-2697(03)00114-3","article-title":"Expression of Viola cyclotides by liquid chromatography-mass spectrometry and tandem mass spectrometry sequencing of intercysteine loops after introduction of charges and cleavage sites by aminoethylation","volume":"318","author":"Goransson","year":"2003","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2005.08.003_bib23","doi-asserted-by":"crossref","first-page":"4531","DOI":"10.1021\/bi002229x","article-title":"Determination of the disulfide structure of sillucin, a highly knotted, cysteine-rich peptide, by cyanylation\/cleavage mass mapping","volume":"40","author":"Qi","year":"2001","journal-title":"Biochemistry"},{"key":"10.1016\/j.ab.2005.08.003_bib24","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.ab.2004.01.037","article-title":"Algorithm-assisted elucidation of disulfide structure: application of the negative signature mass algorithm to mass-mapping the disulfide structure of the 12-cysteine transforming growth factor beta type II receptor extracellular domain","volume":"329","author":"Borges","year":"2004","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2005.08.003_bib25","doi-asserted-by":"crossref","first-page":"35247","DOI":"10.1074\/jbc.M103855200","article-title":"Chloroplast glyceraldehyde-3-phosphate dehydrogenase contains a single disulfide bond located in the C-terminal extension to the B subunit","volume":"276","author":"Qi","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ab.2005.08.003_bib26","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1002\/pro.5560070419","article-title":"Trapping of intermediates during the refolding of recombinant human epidermal growth factor (hEGF) by cyanylation, and subsequent structural elucidation by mass spectrometry","volume":"7","author":"Wu","year":"1998","journal-title":"Protein Sci."},{"key":"10.1016\/j.ab.2005.08.003_bib27","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1021\/pr049961t","article-title":"\u2018Signature sets\u2019, minimal fragment sets for identifying protein disulfide structures with cyanylation-based mass mapping methodology","volume":"3","author":"Wu","year":"2004","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.ab.2005.08.003_bib28","doi-asserted-by":"crossref","first-page":"5655","DOI":"10.1021\/ac000811l","article-title":"A robust, detergent-friendly method for mass spectrometric analysis of integral membrane proteins","volume":"72","author":"Cadene","year":"2000","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ab.2005.08.003_bib29","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/0003-2697(74)90034-7","article-title":"Measurement of protein by spectrophotometry at 205nm","volume":"59","author":"Scopes","year":"1974","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2005.08.003_bib30","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/0003-2697(80)90024-X","article-title":"An absolute method for protein determination based on difference in absorbance at 235 and 280nm","volume":"109","author":"Whitaker","year":"1980","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2005.08.003_bib31","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1016\/S0006-291X(74)80098-7","article-title":"The reaction of cyanogen bromide with S-methylcysteine: fragmentation of the peptide 14\u201329 of bovine pancreatic ribonuclease A","volume":"59","author":"Gross","year":"1974","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.ab.2005.08.003_bib32","series-title":"Use of Statistics to Develop and Evaluate Analytical Methods","first-page":"6","author":"Wernimont","year":"1985"},{"key":"10.1016\/j.ab.2005.08.003_bib33","series-title":"Statistics and Chemometrics for Analytical Chemistry","first-page":"32","author":"Miller","year":"2000"},{"key":"10.1016\/j.ab.2005.08.003_bib34","doi-asserted-by":"crossref","first-page":"2411","DOI":"10.1002\/pro.5560041120","article-title":"How to measure and predict the molar absorption coefficient of a protein","volume":"4","author":"Pace","year":"1995","journal-title":"Protein Sci."},{"key":"10.1016\/j.ab.2005.08.003_bib35","unstructured":"JMP IN software for statistical analysis, Version 5.1, SAS Institute Inc., Cary, NC., 2003."}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269705005646?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269705005646?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T20:40:47Z","timestamp":1757709647000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269705005646"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,11]]},"references-count":35,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2005,11]]}},"alternative-id":["S0003269705005646"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2005.08.003","relation":{},"ISSN":["0003-2697"],"issn-type":[{"type":"print","value":"0003-2697"}],"subject":[],"published":{"date-parts":[[2005,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Study of primary amines for nucleophilic cleavage of cyanylated cystinyl proteins in disulfide mass mapping methodology","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2005.08.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2005 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}