{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T04:40:49Z","timestamp":1782621649943,"version":"3.54.5"},"reference-count":49,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2007,10,1]],"date-time":"2007-10-01T00:00:00Z","timestamp":1191196800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2007,10,1]],"date-time":"2007-10-01T00:00:00Z","timestamp":1191196800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2007,10]]},"DOI":"10.1016\/j.ab.2007.05.014","type":"journal-article","created":{"date-parts":[[2007,5,19]],"date-time":"2007-05-19T16:21:04Z","timestamp":1179591664000},"page":"60-70","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"title":["Development of a cell-based fluorescence resonance energy transfer reporter for Bacillus anthracis lethal factor protease"],"prefix":"10.1016","volume":"369","author":[{"given":"Richard H.","family":"Kimura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin R.","family":"Steenblock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio A.","family":"Camarero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2007.05.014_bib1","doi-asserted-by":"crossref","first-page":"3162","DOI":"10.1073\/pnas.79.10.3162","article-title":"Anthrax toxin edema factor: A bacterial adenylate cyclase that increases cyclic AMP concentrations of eukaryotic cells","volume":"79","author":"Leppla","year":"1982","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ab.2007.05.014_bib2","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1111\/j.1365-2958.1994.tb00500.x","article-title":"Anthrax toxin lethal factor contains a zinc metalloprotease consensus sequence which is required for lethal toxin activity","volume":"13","author":"Klimpel","year":"1994","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.ab.2007.05.014_bib3","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.tibs.2004.04.011","article-title":"Stop the killer: How to inhibit the anthrax lethal factor metalloprotease","volume":"29","author":"Montecucco","year":"2004","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.ab.2007.05.014_bib4","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1038\/n35101999","article-title":"Identification of the cellular receptor for anthrax toxin","volume":"414","author":"Bradley","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.ab.2007.05.014_bib5","doi-asserted-by":"crossref","first-page":"5170","DOI":"10.1073\/pnas.0431098100","article-title":"Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor","volume":"100","author":"Scobie","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ab.2007.05.014_bib6","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1038\/nature02763","article-title":"Crystal structure of a complex between anthrax toxin and its host cell receptor","volume":"430","author":"Santelli","year":"2004","journal-title":"Nature"},{"key":"10.1016\/j.ab.2007.05.014_bib7","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1128\/iai.63.1.82-87.1995","article-title":"Proteolytic activation of bacterial toxins by eukaryotic cells is performed by furin and by additional cellular proteases","volume":"63","author":"Gordon","year":"1995","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ab.2007.05.014_bib8","doi-asserted-by":"crossref","first-page":"20607","DOI":"10.1016\/S0021-9258(17)32036-7","article-title":"Anthrax protective antigen forms oligomers during intoxication of mammalian cells","volume":"269","author":"Milne","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ab.2007.05.014_bib9","doi-asserted-by":"crossref","first-page":"2048","DOI":"10.1126\/science.1073163","article-title":"Macrophage apoptosis by anthrax lethal factor through p38 MAP kinase inhibition","volume":"297","author":"Park","year":"2002","journal-title":"Science"},{"key":"10.1016\/j.ab.2007.05.014_bib10","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1128\/IAI.72.1.430-439.2004","article-title":"Anthrax lethal toxin induces human endothelial cell apoptosis","volume":"72","author":"Kirby","year":"2004","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ab.2007.05.014_bib11","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1038\/nature01794","article-title":"Impairment of dendritic cells and adaptive immunity by anthrax lethal toxin","volume":"424","author":"Agrawal","year":"2003","journal-title":"Nature"},{"key":"10.1016\/j.ab.2007.05.014_bib12","doi-asserted-by":"crossref","first-page":"9499","DOI":"10.1073\/pnas.0502733102","article-title":"Efficient synthetic inhibitors of anthrax lethal factor","volume":"102","author":"Forino","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ab.2007.05.014_bib13","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1016\/j.bmcl.2005.10.088","article-title":"The discovery of a potent and selective lethal factor inhibitor for adjunct therapy of anthrax infection","volume":"16","author":"Xiong","year":"2006","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"10.1016\/j.ab.2007.05.014_bib14","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.tim.2006.01.006","article-title":"Phage display in the study of infectious diseases","volume":"14","author":"Mullen","year":"2006","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.ab.2007.05.014_bib15","doi-asserted-by":"crossref","first-page":"2374","DOI":"10.1128\/IAI.66.5.2374-2378.1998","article-title":"Lethal factor active-site mutations affect catalytic activity in vitro","volume":"66","author":"Hammond","year":"1998","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ab.2007.05.014_bib16","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1126\/science.280.5364.734","article-title":"Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor","volume":"280","author":"Duesbery","year":"1998","journal-title":"Science"},{"key":"10.1016\/j.ab.2007.05.014_bib17","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1042\/bj3520739","article-title":"Susceptibility of mitogen-activated protein kinase kinase family members to proteolysis by anthrax lethal factor","volume":"352","author":"Vitale","year":"2000","journal-title":"Biochem. J."},{"key":"10.1016\/j.ab.2007.05.014_bib18","doi-asserted-by":"crossref","first-page":"6527","DOI":"10.1073\/pnas.112220599","article-title":"Progress in rapid screening of Bacillus anthracis lethal factor activity","volume":"99","author":"Mock","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ab.2007.05.014_bib19","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1038\/418386a","article-title":"Screening inhibitors of anthrax lethal factor","volume":"418","author":"Tonello","year":"2002","journal-title":"Nature"},{"key":"10.1016\/j.ab.2007.05.014_bib20","doi-asserted-by":"crossref","first-page":"3801","DOI":"10.1021\/ol048417n","article-title":"Preparation of peptide p -nitroanilides using an aryl hydrazine resin","volume":"6","author":"Kwon","year":"2004","journal-title":"Org. Lett."},{"key":"10.1016\/j.ab.2007.05.014_bib21","doi-asserted-by":"crossref","first-page":"5232","DOI":"10.1021\/jm0605132","article-title":"Novel small-molecule inhibitors of anthrax lethal factor identified by high-throughput screening","volume":"49","author":"Schepetkin","year":"2006","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.ab.2007.05.014_bib22","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1038\/nmeth819","article-title":"A guide to choosing fluorescent proteins","volume":"2","author":"Shaner","year":"2005","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ab.2007.05.014_bib23","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1126\/science.1124618","article-title":"The fluorescent toolbox for assessing protein location and function","volume":"312","author":"Giepmans","year":"2006","journal-title":"Science"},{"key":"10.1016\/j.ab.2007.05.014_bib24","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1038\/nbt1066","article-title":"Evolutionary optimization of fluorescent proteins for intracellular FRET","volume":"23","author":"Nguyen","year":"2005","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.ab.2007.05.014_bib25","doi-asserted-by":"crossref","first-page":"7583","DOI":"10.1073\/pnas.0511108103","article-title":"Protease specificity determination by using cellular libraries of peptide substrates (CLiPS)","volume":"103","author":"Boulware","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ab.2007.05.014_bib26","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1038\/nmeth0207-112b","article-title":"FRETting for a more detailed interactome","volume":"4","author":"Kaganman","year":"2007","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ab.2007.05.014_bib27","doi-asserted-by":"crossref","first-page":"5597","DOI":"10.1021\/ja990929n","article-title":"Biosynthesis of a head-to-tail cyclized protein with improved biological activity","volume":"121","author":"Camarero","year":"1999","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.ab.2007.05.014_bib28","doi-asserted-by":"crossref","first-page":"13638","DOI":"10.1073\/pnas.96.24.13638","article-title":"Production of cyclic peptides and proteins in vivo","volume":"96","author":"Scott","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ab.2007.05.014_bib29","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1038\/35025116","article-title":"Peptide cyclization catalysed by the thioesterase domain of tyrocidine synthetase","volume":"407","author":"Trauger","year":"2000","journal-title":"Nature"},{"key":"10.1016\/j.ab.2007.05.014_bib30","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1038\/nature00907","article-title":"Biomimetic synthesis and optimization of cyclic peptide antibiotics","volume":"418","author":"Kohli","year":"2002","journal-title":"Nature"},{"key":"10.1016\/j.ab.2007.05.014_bib31","doi-asserted-by":"crossref","first-page":"789","DOI":"10.2174\/0929866054864274","article-title":"Expressed protein ligation: A new tool for the biosynthesis of cyclic polypeptides","volume":"12","author":"Kimura","year":"2005","journal-title":"Protein Peptide Lett."},{"key":"10.1016\/j.ab.2007.05.014_bib32","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1002\/anie.200503882","article-title":"Biosynthesis of the cyclotide kalata B1 by using protein splicing","volume":"45","author":"Kimura","year":"2006","journal-title":"Angew. Chem. Intl. Ed."},{"key":"10.1016\/j.ab.2007.05.014_bib33","doi-asserted-by":"crossref","first-page":"32755","DOI":"10.1074\/jbc.M603614200","article-title":"Retrocyclins kill bacilli and germinating spores of Bacillus anthracis and inactivate anthrax lethal toxin","volume":"281","author":"Wang","year":"2006","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ab.2007.05.014_bib34","doi-asserted-by":"crossref","first-page":"4830","DOI":"10.1073\/pnas.0500508102","article-title":"Human (-defensins neutralize anthrax lethal toxin and protect against its fatal consequences","volume":"102","author":"Kim","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ab.2007.05.014_bib35","doi-asserted-by":"crossref","first-page":"4774","DOI":"10.1021\/ja0495359","article-title":"Inhibition of the proteolytic activity of anthrax lethal factor by aminoglycosides","volume":"126","author":"Lee","year":"2004","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.ab.2007.05.014_bib36","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1038\/nsmb708","article-title":"The structural basis for substrate and inhibitor selectivity of the anthrax lethal factor","volume":"11","author":"Turk","year":"2004","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.ab.2007.05.014_bib37","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1111\/j.1399-3011.1990.tb00939.x","article-title":"Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids","volume":"35","author":"Fields","year":"1990","journal-title":"Intl. J. Peptide Protein Res."},{"key":"10.1016\/j.ab.2007.05.014_bib38","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/0003-2697(81)90704-1","article-title":"Quantitative monitoring of solid-phase peptide synthesis by the ninhydrin reaction","volume":"117","author":"Sarin","year":"1981","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2007.05.014_bib39","doi-asserted-by":"crossref","first-page":"18458","DOI":"10.1073\/pnas.0605422103","article-title":"Intracellular protein interaction mapping with FRET hybrids","volume":"103","author":"You","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ab.2007.05.014_bib40","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1038\/n35101998","article-title":"Crystal structure of the anthrax lethal factor","volume":"414","author":"Pannifer","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.ab.2007.05.014_bib41","doi-asserted-by":"crossref","first-page":"13183","DOI":"10.1021\/bi061288t","article-title":"Quantitative understanding of the energy transfer between fluorescent proteins connected via flexible peptide linkers","volume":"45","author":"Evers","year":"2006","journal-title":"Biochemistry"},{"key":"10.1016\/j.ab.2007.05.014_bib42","unstructured":"E. M. Dongen, T. H. Evers, L. M. Dekkers, E. W. Meijer, L. W. Klomp, M. Merkx, Variation of linker length in ratiometric fluorescent sensor proteins allows rational tuning of Zn(II) affinity in the picomolar to femtomolar range, J. Am. Chem. Soc. (in press)."},{"key":"10.1016\/j.ab.2007.05.014_bib43","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1007\/BF00332615","article-title":"Origin of replication of Escherichia coli plasmid RSF 1030","volume":"187","author":"Som","year":"1982","journal-title":"Mol. Gen. Genet."},{"key":"10.1016\/j.ab.2007.05.014_bib44","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1038\/nmeth851","article-title":"Mapping structural interactions using in-cell NMR spectroscopy (STINT\u2013NMR)","volume":"3","author":"Burz","year":"2006","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ab.2007.05.014_bib45","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0378-1119(98)00020-1","article-title":"Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability","volume":"209","author":"Grossman","year":"1998","journal-title":"Gene"},{"key":"10.1016\/j.ab.2007.05.014_bib46","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/S1046-5928(02)00707-6","article-title":"Preparation of uniformly labeled NMR samples in Escherichia coli under the tight control of the araBAD promoter: Expression of an archaeal homolog of the RNase P Rpp29 protein","volume":"28","author":"Boomershine","year":"2003","journal-title":"Protein Expr. Purif."},{"issue":"Suppl.1","key":"10.1016\/j.ab.2007.05.014_bib47","first-page":"89","article-title":"A cell-based approach for biosynthesis\/screening of cyclic peptide libraries against bacterial toxins","volume":"12","author":"Camarero","year":"2006","journal-title":"J. Peptide Sci."},{"key":"10.1016\/j.ab.2007.05.014_bib48","first-page":"251","article-title":"The cyclotides: Novel macrocyclic peptides as scaffolds in drug design","volume":"5","author":"Craik","year":"2002","journal-title":"Curr. Opin. Drug Discov. Dev."},{"key":"10.1016\/j.ab.2007.05.014_bib49","doi-asserted-by":"crossref","first-page":"351","DOI":"10.2174\/1389203043379594","article-title":"Sunflower trypsin inhibitor-1","volume":"5","author":"Korsinczky","year":"2004","journal-title":"Curr. Protein Peptide Sci."}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269707003284?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269707003284?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T20:47:19Z","timestamp":1757710039000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269707003284"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,10]]},"references-count":49,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["S0003269707003284"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2007.05.014","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2007,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a cell-based fluorescence resonance energy transfer reporter for Bacillus anthracis lethal factor protease","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2007.05.014","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2007 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}