{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T08:20:47Z","timestamp":1775290847902,"version":"3.50.1"},"reference-count":37,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2008,1]]},"DOI":"10.1016\/j.ab.2007.08.034","type":"journal-article","created":{"date-parts":[[2007,9,4]],"date-time":"2007-09-04T17:07:38Z","timestamp":1188925658000},"page":"21-31","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":25,"title":["An assay for uracil in human DNA at baseline: effect of marginal vitamin B6 deficiency"],"prefix":"10.1016","volume":"372","author":[{"given":"Susan T.","family":"Mashiyama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine M.","family":"Hansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esther","family":"Roitman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stella","family":"Sarmiento","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James E.","family":"Leklem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terry D.","family":"Shultz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce N.","family":"Ames","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2007.08.034_bib1","doi-asserted-by":"crossref","first-page":"3290","DOI":"10.1073\/pnas.94.7.3290","article-title":"Folate deficiency causes uracil misincorporation into human DNA and chromosome breakage: Implications for cancer and neuronal damage","volume":"94","author":"Blount","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ab.2007.08.034_bib2","series-title":"Folate, Present Knowledge in Nutrition","author":"Bailey","year":"2001"},{"key":"10.1016\/j.ab.2007.08.034_bib3","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1096\/fasebj.12.14.1491","article-title":"DNA instability (strand breakage, uracil misincorporation, and defective repair) is increased by folic acid depletion in human lymphocytes in vitro","volume":"12","author":"Duthie","year":"1998","journal-title":"FASEB J."},{"key":"10.1016\/j.ab.2007.08.034_bib4","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1038\/nrc886","article-title":"Are vitamin and mineral deficiencies a major cancer risk?","volume":"2","author":"Ames","year":"2002","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.ab.2007.08.034_bib5","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1002\/ajh.2830310103","article-title":"In vitro DNA synthesis by megaloblastic bone marrow: effect of folates and cobalamins on thymidine incorporation and de novo thymidylate synthesis","volume":"31","author":"Das","year":"1989","journal-title":"Am. J. Hematol."},{"key":"10.1016\/j.ab.2007.08.034_bib6","doi-asserted-by":"crossref","first-page":"1656","DOI":"10.1182\/blood.V83.6.1656.1656","article-title":"Bone marrow cells from vitamin B12- and folate-deficient patients misincorporate uracil into DNA","volume":"83","author":"Wickramasinghe","year":"1994","journal-title":"Blood"},{"key":"10.1016\/j.ab.2007.08.034_bib7","doi-asserted-by":"crossref","first-page":"1956","DOI":"10.1073\/pnas.77.4.1956","article-title":"Methotrexate-induced misincorporation of uracil into DNA","volume":"77","author":"Goulian","year":"1980","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ab.2007.08.034_bib8","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/0027-5107(88)90085-1","article-title":"Role of deoxyuridine incorporation and DNA repair in the expression of human chromosomal fragile sites","volume":"200","author":"Reidy","year":"1988","journal-title":"Mutat. Res."},{"key":"10.1016\/j.ab.2007.08.034_bib9","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1056\/NEJM198111053051918","article-title":"Uracil in DNA in megaloblastic anemia","volume":"305","author":"Luzzatto","year":"1981","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.ab.2007.08.034_bib10","series-title":"Biochemistry","author":"Voet","year":"1990"},{"key":"10.1016\/j.ab.2007.08.034_bib11","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S1383-5742(00)00024-7","article-title":"Suppression of spontaneous mutagenesis in human cells by DNA base excision-repair","volume":"462","author":"Lindahl","year":"2000","journal-title":"Mutat. Res."},{"key":"10.1016\/j.ab.2007.08.034_bib12","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1007\/BF00261686","article-title":"Repair of uracil residues closely spaced on the opposite strands of plasmid DNA results in double-strand break and deletion formation","volume":"225","author":"Dianov","year":"1991","journal-title":"Mol. Gen. Genet."},{"key":"10.1016\/j.ab.2007.08.034_bib13","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1093\/ajcn\/65.1.46","article-title":"Folate deficiency in rats induces DNA strand breaks and hypomethylation within the p53 tumor suppressor gene","volume":"65","author":"Kim","year":"1997","journal-title":"Am. J. Clin. Nutr."},{"key":"10.1016\/j.ab.2007.08.034_bib14","first-page":"1089","article-title":"The effect of folic acid deficiency and MTHFR C677T polymorphism on chromosome damage in human lymphocytes in vitro","volume":"10","author":"Crott","year":"2001","journal-title":"Cancer Epidemiol. Biomarkers Prev."},{"key":"10.1016\/j.ab.2007.08.034_bib15","series-title":"Present Knowledge in Nutrition","first-page":"191","article-title":"Chapter 18: Vitamin B-6","author":"Leklem","year":"1996"},{"key":"10.1016\/j.ab.2007.08.034_bib16","unstructured":"Institute of Medicine, DRI Reference Intakes for Thiamine, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Pantothenic Acid, Biotin, and Choline., National Academy Press, Washington, DC, 1998."},{"key":"10.1016\/j.ab.2007.08.034_bib17","unstructured":"A. Moshfegh, J. Goldman, L. Cleveland, What We Eat in America, NHANES 2001-2002: Usual Nutrient Intakes from Food Compared to Dietary Reference Intakes. U.S. Department of Agriculture, Agricultural Research Service, 2005."},{"key":"10.1016\/j.ab.2007.08.034_bib18","doi-asserted-by":"crossref","first-page":"17589","DOI":"10.1073\/pnas.0608757103","article-title":"Low micronutrient intake may accelerate the degenerative diseases of aging through allocation of scarce micronutrients by triage","volume":"103","author":"Ames","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.ab.2007.08.034_bib19","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1159\/000075139","article-title":"Effect of smoking on serum concentrations of total homocysteine, folate, vitamin B12, and nitric oxide in pregnancy: a preliminary study","volume":"19","author":"Ozerol","year":"2004","journal-title":"Fetal. Diagn. Ther."},{"key":"10.1016\/j.ab.2007.08.034_bib20","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/S0015-0282(00)01697-6","article-title":"Low seminal plasma folate concentrations are associated with low sperm density and count in male smokers and nonsmokers","volume":"75","author":"Wallock","year":"2001","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.ab.2007.08.034_bib21","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1093\/ajcn\/71.2.530","article-title":"Ascorbate is depleted by smoking and repleted by moderate supplementation: a study in male smokers and nonsmokers with matched dietary antioxidant intakes","volume":"71","author":"Lykkesfeldt","year":"2000","journal-title":"Am. J. Clin. Nutr."},{"key":"10.1016\/j.ab.2007.08.034_bib22","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.toxlet.2006.09.003","article-title":"DNA damage and repair capacity by comet assay in lymphocytes of white-collar active smokers and passive smokers (non- and ex-smokers) at workplace","volume":"167","author":"Fracasso","year":"2006","journal-title":"Toxicol. Lett."},{"key":"10.1016\/j.ab.2007.08.034_bib23","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.tox.2006.03.001","article-title":"The influence of cigarette smoking on human sperm quality and DNA fragmentation","volume":"223","author":"Sepaniak","year":"2006","journal-title":"Toxicology"},{"key":"10.1016\/j.ab.2007.08.034_bib24","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.ab.2004.03.065","article-title":"Uracil in DNA, determined by an improved assay, is increased when deoxynucleosides are added to folate-deficient cultured human lymphocytes","volume":"330","author":"Mashiyama","year":"2004","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2007.08.034_bib25","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1146\/annurev.nu.16.070196.002441","article-title":"Folate receptors","volume":"16","author":"Antony","year":"1996","journal-title":"Annu. Rev. Nutr."},{"key":"10.1016\/j.ab.2007.08.034_bib26","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1006\/abio.1994.1257","article-title":"Analysis of uracil in DNA by gas chromatography-mass spectrometry","volume":"219","author":"Blount","year":"1994","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2007.08.034_bib27","doi-asserted-by":"crossref","first-page":"2065","DOI":"10.1182\/blood.V87.5.2065.2065","article-title":"Nucleic acid composition of bone marrow mononuclear cells in cobalamin deficiency","volume":"87","author":"Ramsahoye","year":"1996","journal-title":"Blood"},{"key":"10.1016\/j.ab.2007.08.034_bib28","doi-asserted-by":"crossref","first-page":"4617","DOI":"10.1182\/blood.V89.12.4617","article-title":"Apoptosis of late-stage erythroblasts in megaloblastic anemia: association with DNA damage and macrocyte production","volume":"89","author":"Koury","year":"1997","journal-title":"Blood"},{"key":"10.1016\/j.ab.2007.08.034_bib29","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1093\/carcin\/22.7.1019","article-title":"Methylenetetrahydrofolate reductase C677T polymorphism does not alter folic acid deficiency-induced uracil incorporation into primary human lymphocyte DNA in vitro","volume":"22","author":"Crott","year":"2001","journal-title":"Carcinogenesis"},{"key":"10.1016\/j.ab.2007.08.034_bib30","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1021\/ac010556k","article-title":"Uracil in human DNA from subjects with normal and impaired folate status as determined by high-performance liquid chromatography-tandem mass spectrometry","volume":"74","author":"Ren","year":"2002","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ab.2007.08.034_bib31","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.1093\/jn\/133.4.1206","article-title":"Biochemical and molecular aberrations in the rat colon due to folate depletion are age-specific","volume":"133","author":"Choi","year":"2003","journal-title":"J. Nutr."},{"key":"10.1016\/j.ab.2007.08.034_bib32","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/S0891-5849(00)00316-6","article-title":"A simpler, more robust method for the analysis of 8-oxoguanine in DNA","volume":"29","author":"Beckman","year":"2000","journal-title":"Free Radical Biol. Med."},{"key":"10.1016\/j.ab.2007.08.034_bib33","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1093\/ajcn\/61.5.1091","article-title":"Vitamin B-6 requirement and status assessment: young women fed a depletion diet followed by a plant- or animal-protein diet with graded amounts of vitamin B-6","volume":"61","author":"Kretsch","year":"1995","journal-title":"Am. J. Clin. Nutr."},{"key":"10.1016\/j.ab.2007.08.034_bib34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1043-4526(08)60126-6","article-title":"Chemical and nutritional aspects of folate research: analytical procedures, methods of folate synthesis, stability, and bioavailability of dietary folates","volume":"33","author":"Gregory","year":"1989","journal-title":"Adv. Food Nutr. Res."},{"key":"10.1016\/j.ab.2007.08.034_bib35","doi-asserted-by":"crossref","first-page":"1777","DOI":"10.1093\/jn\/131.6.1777","article-title":"Assessment of vitamin B-6 status in young women consuming a controlled diet containing four levels of vitamin B-6 provides an estimated average requirement and recommended dietary allowance","volume":"131","author":"Hansen","year":"2001","journal-title":"J. Nutr."},{"key":"10.1016\/j.ab.2007.08.034_bib36","doi-asserted-by":"crossref","first-page":"3308","DOI":"10.1093\/jn\/132.11.3308","article-title":"Improved vitamin B-6 status is positively related to lymphocyte proliferation in young women consuming a controlled diet","volume":"132","author":"Kwak","year":"2002","journal-title":"J. Nutr."},{"key":"10.1016\/j.ab.2007.08.034_bib37","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1093\/ajcn\/75.4.616","article-title":"High-dose vitamin therapy stimulates variant enzymes with decreased coenzyme binding affinity (increased K(m)): relevance to genetic disease and polymorphisms","volume":"75","author":"Ames","year":"2002","journal-title":"Am. J. Clin. Nutr."}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269707005696?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269707005696?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T20:47:49Z","timestamp":1757710069000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269707005696"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,1]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,1]]}},"alternative-id":["S0003269707005696"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2007.08.034","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2008,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An assay for uracil in human DNA at baseline: effect of marginal vitamin B6 deficiency","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2007.08.034","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2007 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}