{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T23:36:18Z","timestamp":1791070578337,"version":"4.1.0"},"reference-count":26,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2013,11]]},"DOI":"10.1016\/j.ab.2013.07.040","type":"journal-article","created":{"date-parts":[[2013,8,6]],"date-time":"2013-08-06T04:15:39Z","timestamp":1375762539000},"page":"231-236","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["Quantitative affinity electrophoresis of RNA\u2013small molecule interactions by cross-linking the ligand to acrylamide"],"prefix":"10.1016","volume":"442","author":[{"given":"Sherry N.","family":"Boodram","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas C.","family":"McCann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael G.","family":"Organ","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip E.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2013.07.040_b0005","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1146\/annurev.biophys.37.032807.130000","article-title":"Riboswitches: emerging themes in RNA structure and function","volume":"37","author":"Montange","year":"2008","journal-title":"Annu. Rev. Biophys."},{"key":"10.1016\/j.ab.2013.07.040_b0010","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1146\/annurev.biochem.78.070507.135656","article-title":"The structural and functional diversity of metabolite-binding riboswitches","volume":"78","author":"Roth","year":"2009","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.ab.2013.07.040_b0015","doi-asserted-by":"crossref","first-page":"2672","DOI":"10.1002\/anie.200804643","article-title":"The chemical biology of aptamers","volume":"48","author":"Mayer","year":"2009","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.ab.2013.07.040_b0020","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1021\/cr0301088","article-title":"Molecular insights into aminoglycoside action and resistance","volume":"105","author":"Magnet","year":"2005","journal-title":"Chem. Rev."},{"key":"10.1016\/j.ab.2013.07.040_b0025","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1021\/bi9819428","article-title":"Aminoglycoside antibiotics are able to specifically bind the 5\u2032-untranslated region of thymidylate synthase messenger RNA","volume":"38","author":"Tok","year":"1999","journal-title":"Biochemistry"},{"key":"10.1016\/j.ab.2013.07.040_b0030","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1002\/cbic.200300680","article-title":"Targeting RNA with small molecules","volume":"4","author":"Tor","year":"2003","journal-title":"ChemBioChem"},{"key":"10.1016\/j.ab.2013.07.040_b0035","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1021\/cr960415w","article-title":"A structural basis for RNA\u2013ligand interactions","volume":"97","author":"Chow","year":"1997","journal-title":"Chem. Rev."},{"key":"10.1016\/j.ab.2013.07.040_b0040","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1021\/cr0681546","article-title":"Targeting RNA with small molecules","volume":"108","author":"Thomas","year":"2008","journal-title":"Chem. Rev."},{"key":"10.1016\/j.ab.2013.07.040_b0045","doi-asserted-by":"crossref","first-page":"93","DOI":"10.4155\/fmc.09.149","article-title":"Strategies for the design of RNA-binding small molecules","volume":"2","author":"Aboul-Ela","year":"2010","journal-title":"Future Med. Chem."},{"key":"10.1016\/j.ab.2013.07.040_b0050","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.cell.2009.01.035","article-title":"Origins and mechanisms of miRNAs and siRNAs","volume":"136","author":"Carthew","year":"2009","journal-title":"Cell"},{"key":"10.1016\/j.ab.2013.07.040_b0055","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1038\/nature07754","article-title":"On the road to reading the RNA-interference code","volume":"257","author":"Siomi","year":"2009","journal-title":"Nature"},{"key":"10.1016\/j.ab.2013.07.040_b0060","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/j.cell.2009.02.007","article-title":"The centrality of RNA","volume":"136","author":"Sharp","year":"2009","journal-title":"Cell"},{"key":"10.1016\/j.ab.2013.07.040_b0065","doi-asserted-by":"crossref","first-page":"3136","DOI":"10.1021\/ja310742m","article-title":"Microfluidic screening of electrophoretic mobility shifts elucidates riboswitch binding function","volume":"135","author":"Karns","year":"2013","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.ab.2013.07.040_b0070","doi-asserted-by":"crossref","first-page":"1849","DOI":"10.1038\/nprot.2007.249","article-title":"Electrophoretic mobility shift assay (EMSA) for detecting protein\u2013nucleic acid interactions","volume":"2","author":"Hellman","year":"2007","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ab.2013.07.040_b0075","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/978-1-59745-248-9_19","article-title":"Electrophoretic mobility shift assay for characterizing RNA\u2013protein interaction","volume":"703","author":"Gagnon","year":"2011","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.ab.2013.07.040_b0080","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0003-2697(81)90252-9","article-title":"Affinity electrophoresis","volume":"112","author":"Horejsi","year":"1981","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2013.07.040_b0085","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1002\/elps.1150050402","article-title":"Affinity electrophoresis: principles and applications","volume":"5","author":"Takeo","year":"1984","journal-title":"Electrophoresis"},{"key":"10.1016\/j.ab.2013.07.040_b0090","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/S0141-0229(00)00246-5","article-title":"Affinity electrophoresis for the identification and characterization of soluble sugar binding by carbohydrate-binding modules","volume":"27","author":"Tomme","year":"2000","journal-title":"Enzyme Microb. Technol."},{"key":"10.1016\/j.ab.2013.07.040_b0095","first-page":"57","article-title":"Analysis of bacteriophage N protein and peptide binding to boxB RNA using polyacrylamide gel coelectrophoresis (PACE)","volume":"3","author":"Cilley","year":"1997","journal-title":"RNA"},{"key":"10.1016\/j.ab.2013.07.040_b0100","doi-asserted-by":"crossref","first-page":"8844","DOI":"10.1021\/bi000607s","article-title":"Binding site analysis of cellulose binding domain CBDN1 from endoglucanase C of Cellulomonas fimi by site-directed mutagenesis","volume":"39","author":"Kormos","year":"2000","journal-title":"Biochemistry"},{"key":"10.1016\/j.ab.2013.07.040_b0105","doi-asserted-by":"crossref","first-page":"S229","DOI":"10.1002\/elps.200900073","article-title":"Affinity in electrophoresis","volume":"30","author":"Heegaard","year":"2009","journal-title":"Electrophoresis"},{"key":"10.1016\/j.ab.2013.07.040_b0110","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.ab.2010.09.033","article-title":"Identification of RNA\u2013ligand interactions by affinity electrophoresis","volume":"409","author":"Boodram","year":"2011","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2013.07.040_b0115","doi-asserted-by":"crossref","first-page":"8783","DOI":"10.1093\/nar\/15.21.8783","article-title":"Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates","volume":"15","author":"Milligan","year":"1987","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ab.2013.07.040_b0120","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1261\/rna.1514909","article-title":"Structure of the cytosine\u2013cytosine mismatch in the thymidylate synthase mRNA binding site and analysis of its interaction with the aminoglycoside paromomycin","volume":"15","author":"Tavares","year":"2009","journal-title":"RNA"},{"key":"10.1016\/j.ab.2013.07.040_b0125","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/0021-9673(94)01265-G","article-title":"Advances in affinity electrophoresis","volume":"698","author":"Takeo","year":"1995","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.ab.2013.07.040_b0130","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/S0969-2126(01)00629-3","article-title":"Crystal structure of paromomycin docked into the eubacterial ribosomal decoding A site","volume":"9","author":"Vicens","year":"2001","journal-title":"Structure"}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269713003588?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269713003588?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T21:09:32Z","timestamp":1757711372000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269713003588"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["S0003269713003588"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2013.07.040","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2013,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Quantitative affinity electrophoresis of RNA\u2013small molecule interactions by cross-linking the ligand to acrylamide","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2013.07.040","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}