{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:55:58Z","timestamp":1760889358177,"version":"3.44.0"},"reference-count":21,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2017,6]]},"DOI":"10.1016\/j.ab.2017.03.005","type":"journal-article","created":{"date-parts":[[2017,3,6]],"date-time":"2017-03-06T18:19:13Z","timestamp":1488824353000},"page":"1-8","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"special_numbering":"C","title":["Characterization of succinimide stability during trypsin digestion for LC-MS analysis"],"prefix":"10.1016","volume":"526","author":[{"given":"Christine","family":"Nowak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gomathinayagam","family":"Ponniah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alyssa","family":"Neill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongcheng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.ab.2017.03.005_bib1","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/S0021-9258(19)75855-4","article-title":"Deamidation, isomerization, and racemization at asparaginyl and aspartyl residues in peptides. Succinimide-linked reactions that contribute to protein degradation","volume":"262","author":"Geiger","year":"1987","journal-title":"J.\u00a0Biol. Chem."},{"issue":"7","key":"10.1016\/j.ab.2017.03.005_bib2","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1023\/A:1015807303766","article-title":"Chemical pathways of peptide degradation. II. Kinetics of deamidation of an asparaginyl residue in a model hexapeptide","volume":"7","author":"Patel","year":"1990","journal-title":"Pharm. Res."},{"issue":"6","key":"10.1016\/j.ab.2017.03.005_bib3","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1021\/bi951526c","article-title":"Isomerization of an aspartic acid residue in the complementarity-determining regions of a recombinant antibody to human IgE: identification and effect on binding affinity","volume":"35","author":"Cacia","year":"1996","journal-title":"Biochemistry"},{"issue":"6","key":"10.1016\/j.ab.2017.03.005_bib4","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1007\/s11095-007-9241-4","article-title":"Accumulation of succinimide in a recombinant monoclonal antibody in mildly acidic buffers under elevated temperatures","volume":"24","author":"Chu","year":"2007","journal-title":"Pharm. Res."},{"issue":"4","key":"10.1016\/j.ab.2017.03.005_bib5","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.1021\/ac802708s","article-title":"Direct identification and quantification of aspartyl succinimide in an IgG2 mAb by RapiGest assisted digestion","volume":"81","author":"Huang","year":"2009","journal-title":"Anal. Chem."},{"issue":"3","key":"10.1016\/j.ab.2017.03.005_bib6","doi-asserted-by":"crossref","DOI":"10.4161\/mabs.24458","article-title":"Comparison of the in\u00a0vitro and in\u00a0vivo stability of a succinimide intermediate observed on a therapeutic IgG1 molecule","volume":"5","author":"Ouellette","year":"2013","journal-title":"MAbs."},{"issue":"7","key":"10.1016\/j.ab.2017.03.005_bib7","doi-asserted-by":"crossref","first-page":"2714","DOI":"10.1021\/ac0617870","article-title":"18O labeling method for identification and quantification of succinimide in proteins","volume":"79","author":"Xiao","year":"2007","journal-title":"Anal. Chem."},{"issue":"10","key":"10.1016\/j.ab.2017.03.005_bib8","doi-asserted-by":"crossref","first-page":"3509","DOI":"10.1002\/jps.21655","article-title":"Succinimide formation at Asn 55 in the complementarity determining region of a recombinant monoclonal antibody IgG1 heavy chain","volume":"98","author":"Yan","year":"2009","journal-title":"J.\u00a0Pharm. Sci."},{"issue":"15","key":"10.1016\/j.ab.2017.03.005_bib9","doi-asserted-by":"crossref","first-page":"5912","DOI":"10.1021\/ac200750u","article-title":"Accurate determination of succinimide degradation products using high fidelity trypsin digestion peptide map analysis","volume":"83","author":"Yu","year":"2011","journal-title":"Anal. Chem."},{"issue":"9","key":"10.1016\/j.ab.2017.03.005_bib10","doi-asserted-by":"crossref","first-page":"3168","DOI":"10.1021\/ac702245c","article-title":"Separation and characterization of an IgG2 antibody containing a cyclic imide in CDR1 of light chain by hydrophobic interaction chromatography and mass spectrometry","volume":"80","author":"Valliere-Douglass","year":"2008","journal-title":"Anal. Chem."},{"issue":"3","key":"10.1016\/j.ab.2017.03.005_bib11","doi-asserted-by":"crossref","first-page":"1871","DOI":"10.1021\/acs.analchem.5b04276","article-title":"LC-MS\/MS-based monitoring of in\u00a0vivo protein biotransformation: quantitative determination of trastuzumab and its deamidation products in human plasma","volume":"88","author":"Bults","year":"2016","journal-title":"Anal. Chem."},{"issue":"1","key":"10.1016\/j.ab.2017.03.005_bib12","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1002\/pro.5560030119","article-title":"Identification of succinimide sites in proteins by N-terminal sequence analysis after alkaline hydroxylamine cleavage","volume":"3","author":"Kwong","year":"1994","journal-title":"Protein Sci."},{"issue":"1","key":"10.1016\/j.ab.2017.03.005_bib13","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/BF01891996","article-title":"Trapping succinimides in aged polypeptides by chemical reduction","volume":"13","author":"Carter","year":"1994","journal-title":"J.\u00a0Protein Chem."},{"issue":"29","key":"10.1016\/j.ab.2017.03.005_bib14","doi-asserted-by":"crossref","first-page":"8770","DOI":"10.1021\/bi00195a019","article-title":"Isolation and characterization of 101-succinimide lysozyme that possesses the cyclic imide at Asp101-Gly102","volume":"33","author":"Tomizawa","year":"1994","journal-title":"Biochemistry"},{"issue":"3","key":"10.1016\/j.ab.2017.03.005_bib15","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.dci.2004.07.003","article-title":"IMGT unique numbering for immunoglobulin and T cell receptor constant domains and Ig superfamily C-like domains","volume":"29","author":"Lefranc","year":"2005","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.ab.2017.03.005_bib16","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/S0076-6879(99)09018-7","article-title":"Inhibition of stress-induced aggregation of protein therapeutics","volume":"309","author":"Carpenter","year":"1999","journal-title":"Methods Enzymol."},{"issue":"24","key":"10.1016\/j.ab.2017.03.005_bib17","doi-asserted-by":"crossref","first-page":"14142","DOI":"10.1073\/pnas.95.24.14142","article-title":"A\u00a0transient expansion of the native state precedes aggregation of recombinant human interferon-gamma","volume":"95","author":"Kendrick","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"23","key":"10.1016\/j.ab.2017.03.005_bib18","doi-asserted-by":"crossref","first-page":"7107","DOI":"10.1073\/pnas.79.23.7107","article-title":"Nonspecific stabilization of stress-susceptible proteins by stress-resistant proteins: a model for the biological role of heat shock proteins","volume":"79","author":"Minton","year":"1982","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"2","key":"10.1016\/j.ab.2017.03.005_bib19","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.jchromb.2004.12.033","article-title":"Non-enzymatic hinge region fragmentation of antibodies in solution","volume":"818","author":"Cordoba","year":"2005","journal-title":"J.\u00a0Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"issue":"2","key":"10.1016\/j.ab.2017.03.005_bib20","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.imlet.2006.05.011","article-title":"Effect of posttranslational modifications on the thermal stability of a recombinant monoclonal antibody","volume":"106","author":"Liu","year":"2006","journal-title":"Immunol. Lett."},{"issue":"1","key":"10.1016\/j.ab.2017.03.005_bib21","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.jchromb.2008.10.015","article-title":"Assessment of antibody fragmentation by reversed-phase liquid chromatography and mass spectrometry","volume":"876","author":"Liu","year":"2008","journal-title":"J.\u00a0Chromatogr. B Anal. Technol. Biomed. Life Sci."}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269717301148?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269717301148?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T21:18:33Z","timestamp":1757711913000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269717301148"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6]]},"references-count":21,"alternative-id":["S0003269717301148"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2017.03.005","relation":{},"ISSN":["0003-2697"],"issn-type":[{"type":"print","value":"0003-2697"}],"subject":[],"published":{"date-parts":[[2017,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Characterization of succinimide stability during trypsin digestion for LC-MS analysis","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2017.03.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}