{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T12:45:54Z","timestamp":1767962754227,"version":"3.49.0"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2017,12,15]],"date-time":"2017-12-15T00:00:00Z","timestamp":1513296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"CDC Office of Public Health Preparedness and Response"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2018,2]]},"DOI":"10.1016\/j.ab.2017.12.007","type":"journal-article","created":{"date-parts":[[2017,12,8]],"date-time":"2017-12-08T11:14:40Z","timestamp":1512731680000},"page":"97-107","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"special_numbering":"C","title":["Validated MALDI-TOF-MS method for anthrax lethal factor provides early diagnosis and evaluation of therapeutics"],"prefix":"10.1016","volume":"543","author":[{"given":"Maribel","family":"Gallegos-Candela","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne E.","family":"Boyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian R.","family":"Woolfitt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judy","family":"Brumlow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato C.","family":"Lins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Conrad P.","family":"Quinn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex R.","family":"Hoffmaster","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel","family":"Meister","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John R.","family":"Barr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2017.12.007_bib1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1046\/j.1462-5822.2003.00253.x","article-title":"Anthrax toxins and the host: a story of intimacy","volume":"5","author":"Mock","year":"2003","journal-title":"Cell Microbiol."},{"key":"10.1016\/j.ab.2017.12.007_bib2","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1016\/S0140-6736(10)60573-9","article-title":"Anthrax infection in drug users","volume":"375","author":"Booth","year":"2010","journal-title":"Lancet"},{"key":"10.1016\/j.ab.2017.12.007_bib3","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1111\/j.1749-6632.1980.tb18910.x","article-title":"Inhalation anthrax","volume":"353","author":"Brachman","year":"1980","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.ab.2017.12.007_bib4","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.3201\/eid0810.020353","article-title":"Investigation of bioterrorism-related anthrax, United States, 2001: epidemiologic findings","volume":"8","author":"Jernigan","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.ab.2017.12.007_bib5","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1146\/annurev.micro.55.1.647","article-title":"Anthrax","volume":"55","author":"Mock","year":"2001","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.ab.2017.12.007_bib6","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1146\/annurev.biochem.75.103004.142728","article-title":"Anthrax toxin: receptor binding, internalization, pore formation, and translocation","volume":"76","author":"Young","year":"2007","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.ab.2017.12.007_bib7","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1126\/science.280.5364.734","article-title":"Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor","volume":"280","author":"Duesbery","year":"1998","journal-title":"Science"},{"issue":"Pt 3","key":"10.1016\/j.ab.2017.12.007_bib8","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1042\/bj3520739","article-title":"Susceptibility of mitogen-activated protein kinase kinase family members to proteolysis by anthrax lethal factor","volume":"352","author":"Vitale","year":"2000","journal-title":"Biochem J"},{"key":"10.1016\/j.ab.2017.12.007_bib9","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/S0014-5793(99)01502-1","article-title":"Anthrax lethal factor cleaves MKK3 in macrophages and inhibits the LPS\/IFNgamma-induced release of NO and TNFalpha","volume":"462","author":"Pellizzari","year":"1999","journal-title":"FEBS Lett"},{"key":"10.1016\/j.ab.2017.12.007_bib10","doi-asserted-by":"crossref","first-page":"3162","DOI":"10.1073\/pnas.79.10.3162","article-title":"Anthrax toxin edema factor: a bacterial adenylate cyclase that increases cyclic AMP concentrations of eukaryotic cells","volume":"79","author":"Leppla","year":"1982","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ab.2017.12.007_bib11","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1086\/512372","article-title":"A case of naturally acquired inhalation anthrax: clinical care and analyses of anti-protective antigen immunoglobulin G and lethal factor","volume":"44","author":"Walsh","year":"2007","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.ab.2017.12.007_bib12","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1128\/CVI.00288-12","article-title":"Development of an inhalational Bacillus anthracis exposure therapeutic model in cynomolgus macaques","volume":"19","author":"Henning","year":"2012","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.ab.2017.12.007_bib13","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1128\/CVI.00023-06","article-title":"Detection of anthrax toxin in the serum of animals infected with Bacillus anthracis by using engineered immunoassays","volume":"13","author":"Mabry","year":"2006","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.ab.2017.12.007_bib14","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.jim.2014.05.008","article-title":"Detection of anthrax protective antigen (PA) using europium labeled anti-PA monoclonal antibody and time-resolved fluorescence","volume":"408","author":"Stoddard","year":"2014","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.ab.2017.12.007_bib15","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1128\/CVI.00412-08","article-title":"Detection of anthrax toxin by an ultrasensitive immunoassay using europium nanoparticles","volume":"16","author":"Tang","year":"2009","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.ab.2017.12.007_bib16","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.ab.2007.05.014","article-title":"Development of a cell-based fluorescence resonance energy transfer reporter for Bacillus anthracis lethal factor protease","volume":"369","author":"Kimura","year":"2007","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2017.12.007_bib17","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/S0003-2697(03)00424-X","article-title":"An enzymatic electrochemiluminescence assay for the lethal factor of anthrax","volume":"321","author":"Rivera","year":"2003","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2017.12.007_bib18","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1038\/nsmb708","article-title":"The structural basis for substrate and inhibitor selectivity of the anthrax lethal factor","volume":"11","author":"Turk","year":"2004","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.ab.2017.12.007_bib19","doi-asserted-by":"crossref","first-page":"8463","DOI":"10.1021\/ac701741s","article-title":"Detection and quantification of anthrax lethal factor in serum by mass spectrometry","volume":"79","author":"Boyer","year":"2007","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ab.2017.12.007_bib20","doi-asserted-by":"crossref","first-page":"3432","DOI":"10.1128\/IAI.00346-09","article-title":"Kinetics of lethal factor and poly-D-glutamic acid antigenemia during inhalation anthrax in rhesus macaques","volume":"77","author":"Boyer","year":"2009","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ab.2017.12.007_bib21","series-title":"Biosafety in Microbiological and Biomedical Laboratories","year":"2009"},{"key":"10.1016\/j.ab.2017.12.007_bib22","doi-asserted-by":"crossref","first-page":"2391","DOI":"10.3390\/molecules16032391","article-title":"Quantitative mass spectrometry for bacterial protein toxins\u2013a sensitive, specific, high-throughput tool for detection and diagnosis","volume":"16","author":"Boyer","year":"2011","journal-title":"Molecules"},{"key":"10.1016\/j.ab.2017.12.007_bib23","doi-asserted-by":"crossref","first-page":"1760","DOI":"10.1021\/ac1030144","article-title":"Comparison of MALDI-TOF-MS and HPLC-ESI-MS\/MS for Endopeptidase Activity-Based Quantification of Anthrax Lethal Factor in Serum","volume":"83","author":"Kuklenyik","year":"2011","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ab.2017.12.007_bib24","doi-asserted-by":"crossref","first-page":"2847","DOI":"10.1007\/s00216-015-8509-5","article-title":"High-sensitivity MALDI-TOF MS quantification of anthrax lethal toxin for diagnostics and evaluation of medical countermeasures","volume":"407","author":"Boyer","year":"2015","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.ab.2017.12.007_bib25","doi-asserted-by":"crossref","DOI":"10.2807\/ese.13.51.19076-en","article-title":"Investigations and control measures following a case of inhalation anthrax in East London in a drum maker and drummer, October 2008","volume":"13","author":"Anaraki","year":"2008","journal-title":"Euro Surveill."},{"key":"10.1016\/j.ab.2017.12.007_bib26","first-page":"872","article-title":"C. Centers for disease, prevention, gastrointestinal anthrax after an animal-hide drumming event - New Hampshire and Massachusetts, 2009","volume":"59","year":"2010","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"10.1016\/j.ab.2017.12.007_bib27","doi-asserted-by":"crossref","first-page":"56","DOI":"10.3201\/eid2301.160608","article-title":"Analysis of Anthrax Immune Globulin Intravenous with Antimicrobial Treatment in Injection Drug Users, Scotland, 2009-2010","volume":"23","author":"Cui","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.ab.2017.12.007_bib28","doi-asserted-by":"crossref","first-page":"310","DOI":"10.3201\/eid2002.130245","article-title":"Lethal factor and anti-protective antigen IgG levels associated with inhalation anthrax, Minnesota, USA","volume":"20","author":"Sprenkle","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.ab.2017.12.007_bib29","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1093\/infdis\/jir543","article-title":"Lethal factor toxemia and anti-protective antigen antibody activity in naturally acquired cutaneous anthrax","volume":"204","author":"Boyer","year":"2011","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.ab.2017.12.007_bib30","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1056\/NEJMoa0810603","article-title":"Raxibacumab for the treatment of inhalational anthrax","volume":"361","author":"Migone","year":"2009","journal-title":"N. Engl. J. Med."},{"issue":"Suppl 1","key":"10.1016\/j.ab.2017.12.007_bib31","first-page":"S49","article-title":"Limit of blank, limit of detection and limit of quantitation, The Clinical biochemist","volume":"29","author":"Armbruster","year":"2008","journal-title":"Rev.\/Aust. Assoc. Clin. Biochem."},{"issue":"a","key":"10.1016\/j.ab.2017.12.007_bib32","article-title":"42 U.S.C","volume":"263","author":"Public Health Service Act","year":"1999","journal-title":"Sect"},{"key":"10.1016\/j.ab.2017.12.007_bib33","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0146021","article-title":"Dose-response analysis using R","volume":"10","author":"Ritz","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.ab.2017.12.007_bib34","series-title":"CVM, Guidance for Industry","author":"HHS-FDA","year":"2013"},{"key":"10.1016\/j.ab.2017.12.007_bib35","doi-asserted-by":"crossref","first-page":"3846","DOI":"10.1128\/IAI.01145-10","article-title":"The poly-gamma-D-glutamic acid capsule of Bacillus anthracis enhances lethal toxin activity","volume":"79","author":"Jang","year":"2011","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ab.2017.12.007_bib36","series-title":"Interscience Conference on Antimicrobial Agents and Chemotherapy","article-title":"1000-a-Day: Rapid anthrax diagnostics with high-throughput analysis of anthrax lethal factor by mass spectrometry","author":"Heitz","year":"2014"}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269717305006?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269717305006?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T21:22:05Z","timestamp":1757712125000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269717305006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2]]},"references-count":36,"alternative-id":["S0003269717305006"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2017.12.007","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2018,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Validated MALDI-TOF-MS method for anthrax lethal factor provides early diagnosis and evaluation of therapeutics","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2017.12.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}