{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T23:04:07Z","timestamp":1777417447260,"version":"3.51.4"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T00:00:00Z","timestamp":1527811200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["03IS2201A"],"award-info":[{"award-number":["03IS2201A"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["03IS2201B"],"award-info":[{"award-number":["03IS2201B"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2018,6]]},"DOI":"10.1016\/j.ab.2018.04.014","type":"journal-article","created":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T22:34:41Z","timestamp":1524004481000},"page":"54-60","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"special_numbering":"C","title":["A recombinase polymerase amplification assay for the diagnosis of atypical pneumonia"],"prefix":"10.1016","volume":"550","author":[{"given":"Sebastian","family":"Kersting","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Rausch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank F.","family":"Bier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"von Nickisch-Rosenegk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2018.04.014_bib1","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.1128\/JCM.44.4.1440-1446.2006","article-title":"Simultaneous detection of pathogens in clinical samples from patients with community-acquired pneumonia by real-time PCR with pathogen-specific molecular beacon probes","volume":"44","author":"Morozumi","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ab.2018.04.014_bib2","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1097\/MD.0b013e31827f6104","article-title":"Community-acquired Legionella pneumophila pneumonia: a single-center experience with 214 hospitalized sporadic cases over 15 years","volume":"92","author":"Viasus","year":"2013","journal-title":"Medicine (Baltimore)"},{"key":"10.1016\/j.ab.2018.04.014_bib3","doi-asserted-by":"crossref","DOI":"10.2807\/ese.13.38.18983-en","article-title":"Changes in prevention and outbreak management of Legionnaires disease in The Netherlands between two large outbreaks in 1999 and 2006","volume":"13","author":"Sonder","year":"2008","journal-title":"Euro Surveill."},{"key":"10.1016\/j.ab.2018.04.014_bib4","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1007\/s00103-011-1362-6","article-title":"Interdisziplin\u00e4res Management eines l\u00e4nder\u00fcbergreifenden Legionellenausbruchs","volume":"54","author":"Freudenmann","year":"2011","journal-title":"Bundesgesundheitsblatt - Gesundheitsforsch. - Gesundheitsschutz"},{"key":"10.1016\/j.ab.2018.04.014_bib5","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1017\/S0950268807009077","article-title":"A large Legionnaires' disease outbreak in Pamplona, Spain: early detection, rapid control and no case fatality","volume":"136","author":"Castilla","year":"2008","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.ab.2018.04.014_bib6","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1007\/BF01695659","article-title":"Delay in appropriate therapy of Legionella pneumonia associated with increased mortality","volume":"15","author":"Heath","year":"1996","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.ab.2018.04.014_bib7","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1128\/JCM.41.1.63-66.2003","article-title":"Evaluation of a PCR assay for detection of streptococcus pneumoniae in respiratory and nonrespiratory samples from adults with community-acquired pneumonia","volume":"41","author":"Murdoch","year":"2003","journal-title":"J. Clin. Microbiol."},{"issue":"Suppl 3","key":"10.1016\/j.ab.2018.04.014_bib8","doi-asserted-by":"crossref","first-page":"S202","DOI":"10.1086\/591405","article-title":"Novel approaches to the identification of Streptococcus pneumoniae as the cause of community-acquired pneumonia","volume":"47","author":"Klugman","year":"2008","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.ab.2018.04.014_bib9","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1086\/345852","article-title":"Evaluation of the immunochromatographic binax NOW assay for detection of streptococcus pneumoniae urinary antigen in a prospective study of community-acquired pneumonia in Spain","volume":"36","author":"Gutierrez","year":"2003","journal-title":"Clin. Infect. Dis."},{"issue":"Suppl 4","key":"10.1016\/j.ab.2018.04.014_bib10","doi-asserted-by":"crossref","first-page":"S331","DOI":"10.1093\/cid\/cir048","article-title":"Interpreting assays for the detection of Streptococcus pneumoniae","volume":"52","author":"Blaschke","year":"2011","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.ab.2018.04.014_bib11","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/0732-8893(96)00010-7","article-title":"Legionella urinary antigen testing: potential impact on diagnosis and antibiotic therapy","volume":"24","author":"Kashuba","year":"1996","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.ab.2018.04.014_bib12","doi-asserted-by":"crossref","first-page":"3327","DOI":"10.1128\/JCM.41.7.3327-3330.2003","article-title":"Detection of Legionella pneumophila by real-time PCR for the mip gene","volume":"41","author":"Wilson","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ab.2018.04.014_bib13","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1086\/345529","article-title":"Diagnosis of Legionella infection","volume":"36","author":"Murdoch","year":"2003","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.ab.2018.04.014_bib14","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1128\/JCM.43.4.1581-1586.2005","article-title":"Loop-mediated isothermal amplification method targeting the lytA gene for detection of Streptococcus pneumoniae","volume":"43","author":"Seki","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ab.2018.04.014_bib15","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/s00253-011-3496-8","article-title":"LAMP-based method for a rapid identification of Legionella spp. and Legionella pneumophila","volume":"92","author":"Lu","year":"2011","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.ab.2018.04.014_bib16","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1038\/sj.embor.7400200","article-title":"Helicase-dependent isothermal DNA amplification","volume":"5","author":"Vincent","year":"2004","journal-title":"EMBO Rep."},{"key":"10.1016\/j.ab.2018.04.014_bib17","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.0040204","article-title":"DNA detection using recombination proteins","volume":"4","author":"Piepenburg","year":"2006","journal-title":"PLoS Biol."},{"key":"10.1016\/j.ab.2018.04.014_bib18","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.jcv.2012.05.006","article-title":"Recombinase polymerase amplification assay for rapid detection of Rift Valley fever virus","volume":"54","author":"Euler","year":"2012","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.ab.2018.04.014_bib19","doi-asserted-by":"crossref","first-page":"2234","DOI":"10.1128\/JCM.06504-11","article-title":"Recombinase polymerase amplification assay for rapid detection of francisella tularensis","volume":"50","author":"Euler","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ab.2018.04.014_bib20","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00135-13","article-title":"Rapid detection of HIV-1 proviral DNA for early infant diagnosis using recombinase polymerase amplification","volume":"4","author":"Boyle","year":"2013","journal-title":"mBio"},{"key":"10.1016\/j.ab.2018.04.014_bib21","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.jviromet.2013.06.027","article-title":"A new approach for diagnosis of bovine coronavirus using a reverse transcription recombinase polymerase amplification assay","volume":"193","author":"Amer","year":"2013","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.ab.2018.04.014_bib22","article-title":"Reverse transcription recombinase polymerase amplification assay for the detection of middle East respiratory syndrome coronavirus","volume":"5","author":"Abd El Wahed","year":"2013","journal-title":"PLoS. Curr."},{"key":"10.1016\/j.ab.2018.04.014_bib23","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.jmoldx.2013.08.003","article-title":"Sensitive and rapid detection of Chlamydia trachomatis by recombinase polymerase amplification directly from urine samples","volume":"16","author":"Kr\u00f5lov","year":"2014","journal-title":"J. Mol. Diagn."},{"key":"10.1016\/j.ab.2018.04.014_bib24","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1007\/s00604-014-1198-5","article-title":"Multiplex isothermal solid-phase recombinase polymerase amplification for the specific and fast DNA-based detection of three bacterial pathogens","volume":"181","author":"Kersting","year":"2014","journal-title":"Microchim. Acta."},{"key":"10.1016\/j.ab.2018.04.014_bib25","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1039\/b921140c","article-title":"Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA)","volume":"10","author":"Lutz","year":"2010","journal-title":"Lab Chip"},{"key":"10.1016\/j.ab.2018.04.014_bib26","doi-asserted-by":"crossref","first-page":"4576","DOI":"10.1039\/c2lc40765e","article-title":"A phaseguided passive batch microfluidic mixing chamber for isothermal amplification","volume":"12","author":"Hakenberg","year":"2012","journal-title":"Lab Chip"},{"key":"10.1016\/j.ab.2018.04.014_bib27","doi-asserted-by":"crossref","first-page":"3082","DOI":"10.1039\/c2lc40423k","article-title":"A paper and plastic device for performing recombinase polymerase amplification of HIV DNA","volume":"12","author":"Rohrman","year":"2012","journal-title":"Lab Chip"},{"key":"10.1016\/j.ab.2018.04.014_bib28","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1186\/1475-2875-13-99","article-title":"Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis","volume":"13","author":"Kersting","year":"2014","journal-title":"Malar. J."},{"key":"10.1016\/j.ab.2018.04.014_bib29","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.1021\/acs.analchem.5b03267","article-title":"Multiplexed recombinase polymerase amplification assay to detect intestinal protozoa","volume":"88","author":"Crannell","year":"2016","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ab.2018.04.014_bib30","doi-asserted-by":"crossref","first-page":"37732","DOI":"10.1038\/srep37732","article-title":"Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay","volume":"6","author":"Jauset-Rubio","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ab.2018.04.014_bib31","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1186\/s12879-015-1212-5","article-title":"Development of a rapid recombinase polymerase amplification assay for the detection of Streptococcus pneumoniae in whole blood","volume":"15","author":"Clancy","year":"2015","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.ab.2018.04.014_bib32","doi-asserted-by":"crossref","first-page":"2711","DOI":"10.1128\/AEM.66.7.2711-2717.2000","article-title":"Development of a novel, rapid integrated Cryptosporidium parvum detection assay","volume":"66","author":"Kozwich","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ab.2018.04.014_bib33","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ab.2018.04.014_bib34","doi-asserted-by":"crossref","first-page":"4215","DOI":"10.1128\/JCM.38.11.4215-4218.2000","article-title":"Detection of Legionella pneumophila using a real-time PCR hybridization assay","volume":"38","author":"Ballard","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ab.2018.04.014_bib35","doi-asserted-by":"crossref","first-page":"2618","DOI":"10.1128\/JCM.39.7.2618-2626.2001","article-title":"Direct detection of legionella species from bronchoalveolar lavage and open lung biopsy specimens: comparison of lightcycler PCR, in situ hybridization, Direct fluorescence antigen detection, and culture","volume":"39","author":"Hayden","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ab.2018.04.014_bib36","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1128\/jcm.33.5.1247-1252.1995","article-title":"Enzyme-linked immunoassay for detection of PCR-amplified DNA of legionellae in bronchoalveolar fluid","volume":"33","author":"Jonas","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ab.2018.04.014_bib37","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.diagmicrobio.2004.04.013","article-title":"Comparison of four polymerase chain reaction assays for specificity in the identification of Streptococcus pneumoniae","volume":"49","author":"Messmer","year":"2004","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.ab.2018.04.014_bib38","doi-asserted-by":"crossref","first-page":"8769","DOI":"10.1039\/c3cs60144g","article-title":"Portable nucleic acid thermocyclers","volume":"42","author":"Almassian","year":"2013","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.ab.2018.04.014_bib39","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1128\/JCM.36.6.1560-1567.1998","article-title":"Sequence-based classification scheme for the genus Legionella targeting the mip gene","volume":"36","author":"Ratcliff","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ab.2018.04.014_bib40","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1128\/JCM.02498-06","article-title":"Evaluation and improvement of real-time PCR Assays targeting lytA, ply, and psaA genes for detection of pneumococcal DNA","volume":"45","author":"Carvalho","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ab.2018.04.014_bib41","doi-asserted-by":"crossref","first-page":"4551","DOI":"10.1128\/IAI.67.9.4551-4556.1999","article-title":"The autolysin-encoding gene (lytA) of Streptococcus pneumoniae displays restricted allelic variation despite localized recombination events with genes of pneumococcal bacteriophage encoding cell wall lytic enzymes","volume":"67","author":"Whatmore","year":"1999","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ab.2018.04.014_bib42","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1093\/infdis\/152.2.356","article-title":"Water-related nosocomial pneumonia caused by Legionella pneumophila serogroups 1 and 10","volume":"152","author":"Meenhorst","year":"1985","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.ab.2018.04.014_bib43","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1039\/C1LC20693A","article-title":"Highly-integrated lab-on-chip system for point-of-care multiparameter analysis","volume":"12","author":"Schumacher","year":"2012","journal-title":"Lab Chip"}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269718304020?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269718304020?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T21:22:24Z","timestamp":1757712144000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269718304020"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6]]},"references-count":43,"alternative-id":["S0003269718304020"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2018.04.014","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2018,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A recombinase polymerase amplification assay for the diagnosis of atypical pneumonia","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2018.04.014","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}