{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:20:41Z","timestamp":1790011241810,"version":"4.0.1"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100009571","name":"Universidad Nacional de San Luis","doi-asserted-by":"publisher","award":["PROICO-1512-22\/Q232"],"award-info":[{"award-number":["PROICO-1512-22\/Q232"]}],"id":[{"id":"10.13039\/100009571","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT-2015-2246"],"award-info":[{"award-number":["PICT-2015-2246"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT-2015-1575"],"award-info":[{"award-number":["PICT-2015-1575"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT-2014-1184"],"award-info":[{"award-number":["PICT-2014-1184"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT-2013-3092"],"award-info":[{"award-number":["PICT-2013-3092"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PIDC-0025-FONCyT-MINCyT"],"award-info":[{"award-number":["PIDC-0025-FONCyT-MINCyT"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP- 11220150100004CO"],"award-info":[{"award-number":["PIP- 11220150100004CO"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2019,1]]},"DOI":"10.1016\/j.ab.2018.10.025","type":"journal-article","created":{"date-parts":[[2018,10,28]],"date-time":"2018-10-28T17:33:24Z","timestamp":1540748004000},"page":"116-122","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":33,"special_numbering":"C","title":["Serological diagnosis of Toxoplasmosis disease using a fluorescent immunosensor with chitosan-ZnO-nanoparticles"],"prefix":"10.1016","volume":"564-565","author":[{"given":"Victoria","family":"Medawar-Aguilar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio F.","family":"Jofre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mart\u00edn A.","family":"Fern\u00e1ndez-Baldo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Alonso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Angel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio","family":"Raba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirley V.","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6274-2662","authenticated-orcid":false,"given":"Germ\u00e1n A.","family":"Messina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2018.10.025_bib1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2013\/370769","article-title":"The importance of IgG avidity and the polymerase chain reaction in treating toxoplasmosis during pregnancy: current knowledge","author":"Bortoletti","year":"2013","journal-title":"Interdiscip. Perspect. Infect. Dis."},{"key":"10.1016\/j.ab.2018.10.025_bib2","first-page":"89","article-title":"Congenital Toxoplasma gondii infection","volume":"21","author":"Nazan","year":"2008","journal-title":"Marmara Med. J."},{"key":"10.1016\/j.ab.2018.10.025_bib3","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1017\/S0950268803001948","article-title":"Duration of the IgM response in women acquiring Toxoplasma gondii during pregnancy: implications for clinical practice and cross-sectional incidence studies","volume":"132","author":"Gras","year":"2004","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.ab.2018.10.025_bib4","doi-asserted-by":"crossref","first-page":"246","DOI":"10.5799\/ahinjs.01.2014.02.0396","article-title":"Evaluation of the Toxoplasma gondii IgG Avidity request and results in a tertiary care hospital","volume":"5","author":"G\u00fcng\u00f6r","year":"2014","journal-title":"J. Clin. Exp. Invest."},{"key":"10.1016\/j.ab.2018.10.025_bib5","first-page":"180","article-title":"Diagnosis of toxoplasmosis","volume":"61","author":"Akarsu","year":"2008","journal-title":"Ank. \u00dcni. T\u0131p Fak. Mecm."},{"key":"10.1016\/j.ab.2018.10.025_bib6","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1099\/0022-1317-50-1-62","article-title":"Evaluation of serological markers for the immunodiagnosis of acute acquired toxoplasmosis","volume":"50","author":"Suzuki","year":"2001","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.ab.2018.10.025_bib7","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.vetpar.2006.05.008","article-title":"Comparison of detection methods for Toxoplasma gondii in naturally and experimentally infected swine","volume":"141","author":"Hill","year":"2006","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ab.2018.10.025_bib8","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1038\/nature05064","article-title":"Microfluidic diagnostic technologies for global public health","volume":"442","author":"Yager","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.ab.2018.10.025_bib9","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.talanta.2012.10.042","article-title":"Development of optical immunosensors for detection of proteins in serum","volume":"103","author":"Kyprianou","year":"2012","journal-title":"Talanta"},{"key":"10.1016\/j.ab.2018.10.025_bib10","series-title":"Medical Biosensors for Point of Care (POC) Applications","first-page":"255","article-title":"Microfluidic platforms for point of care (POC) medical diagnostics","author":"Azhar","year":"2017"},{"key":"10.1016\/j.ab.2018.10.025_bib11","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/978-1-61779-806-1_9","article-title":"Fluorescence-based biosensors","volume":"875","author":"Strianese","year":"2012","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.ab.2018.10.025_bib12","doi-asserted-by":"crossref","first-page":"4890","DOI":"10.3390\/s150304890","article-title":"Ultra-sensitive nanofiber fluorescence detection in a microfluidic chip","volume":"15","author":"Li","year":"2015","journal-title":"Sensors"},{"key":"10.1016\/j.ab.2018.10.025_bib13","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.saa.2015.02.025","article-title":"The use of laser-induced fluorescence or ultraviolet detectors for sensitive and selective analysis of tobramycin or erythropoietin in complex samples","volume":"143","author":"Ahmed","year":"2015","journal-title":"Spectrochim. Acta A: Mol. Biomol. Spectrosc."},{"key":"10.1016\/j.ab.2018.10.025_bib14","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.cll.2006.12.010","article-title":"A low-cost, low-power consumption, miniature laser induced fluorescence system for DNA detection on a microfluidic device","volume":"27","author":"Shrinivasan","year":"2007","journal-title":"Clin. Lab. Med."},{"key":"10.1016\/j.ab.2018.10.025_bib15","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1021\/ac5039863","article-title":"Electrochemical sensors and biosensors based on nanomaterials and nanostructures","volume":"87","author":"Zhu","year":"2015","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ab.2018.10.025_bib16","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.talanta.2014.12.026","article-title":"Copper nanoparticles applied to the preconcentration and electrochemical determination of \u03b2-adrenergic agonist: an efficient tool for the control of meat production","volume":"135","author":"Regiart","year":"2015","journal-title":"Talanta"},{"key":"10.1016\/j.ab.2018.10.025_bib17","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.ab.2014.06.016","article-title":"Silica nanoparticle-based microfluidic immunosensor with laser-induced fluorescence detection for the quantification of immunoreactive trypsin","volume":"463","author":"Seia","year":"2014","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2018.10.025_bib18","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1039\/c2an36500f","article-title":"Nanoparticle-based immunoassays in the biomedical field","volume":"138","author":"Tang","year":"2013","journal-title":"Analyst"},{"key":"10.1016\/j.ab.2018.10.025_bib19","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.microc.2016.03.012","article-title":"Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determination","volume":"128","author":"Fern\u00e1ndez-Baldo","year":"2016","journal-title":"Microchem. J."},{"key":"10.1016\/j.ab.2018.10.025_bib20","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.aca.2017.01.029","article-title":"Microfluidic immunosensor based on mesoporous silica platform and CMK-3\/poly-acrylamide-co-methacrylate of dihydrolipoic acid modified gold electrode for cancer biomarker detection","volume":"963","author":"Regiart","year":"2017","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.ab.2018.10.025_bib21","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1016\/j.microc.2016.10.027","article-title":"Fluorescent immunosensor using AP-SNs and QDs for quantitation of IgG anti-Toxocara canis","volume":"130","author":"Medawar","year":"2017","journal-title":"Microchem. J."},{"key":"10.1016\/j.ab.2018.10.025_bib22","doi-asserted-by":"crossref","first-page":"233106","DOI":"10.1063\/1.2210078","article-title":"Zinc oxide nanocomb biosensor for glucose detection","volume":"88","author":"Wang","year":"2006","journal-title":"Appl. Phys. Lett."},{"key":"10.1016\/j.ab.2018.10.025_bib23","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.snb.2012.04.026","article-title":"Laser-induced fluorescence integrated in a microfluidic immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori","volume":"168","author":"Seia","year":"2012","journal-title":"Sens. Actuators, B"},{"key":"10.1016\/j.ab.2018.10.025_bib24","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1080\/00032710701792612","article-title":"Nanostructured zinc oxide particles in chemically modified electrodes for biosensor applications","volume":"41","author":"Kumar","year":"2008","journal-title":"Anal. Lett."},{"key":"10.1016\/j.ab.2018.10.025_bib25","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.snb.2014.02.005","article-title":"Chitosan-stabilized silver nanoparticles for voltammetric detection of nitrocompounds","volume":"196","author":"de Lima","year":"2014","journal-title":"Sens. Actuators, B"},{"key":"10.1016\/j.ab.2018.10.025_bib26","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1163\/156856209X410102","article-title":"Fabrication, characterization of chitosan\/nanosilver film and its potential antibacterial application","volume":"20","author":"Thomas","year":"2009","journal-title":"J. Biomater. Sci. Polym."},{"key":"10.1016\/j.ab.2018.10.025_bib27","doi-asserted-by":"crossref","first-page":"1677","DOI":"10.1016\/S0278-6915(02)00103-5","article-title":"A colorimetric and fluorometric microplate assay for the detection of microcystin-LR in drinking water without preconcentration","volume":"40","author":"Bouaicha","year":"2002","journal-title":"Food Chem. Toxicol."},{"key":"10.1016\/j.ab.2018.10.025_bib28","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1016\/j.soilbio.2005.01.008","article-title":"Performance of para-nitrophenyl phosphate and 4-methylumbelliferyl phosphate as substrate analogues for phosphomonoesterase in soils with different organic matter content","volume":"37","author":"Drouillon","year":"2005","journal-title":"Soil Biol. Biochem."},{"key":"10.1016\/j.ab.2018.10.025_bib29","series-title":"Instruction Manual Anti-Toxoplasma gondii IgG Human ELISA Kit","year":"2013"},{"key":"10.1016\/j.ab.2018.10.025_bib30","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1021\/ac00058a029","article-title":"Planar glass chips for capillary electrophoresis: repetitive sample injection, quantitation, and separation efficiency","volume":"65","author":"Seiler","year":"1993","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ab.2018.10.025_bib31","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.jim.2007.12.019","article-title":"A reusable piezoelectric immunosensor using antibody-adsorbed magnetic nanocomposite","volume":"332","author":"Zhang","year":"2008","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.ab.2018.10.025_bib32","first-page":"60","article-title":"Focus Beads and chips: new recipes for analysis","volume":"3","author":"Verpoorte","year":"2003","journal-title":"Lab a Chip"},{"key":"10.1016\/j.ab.2018.10.025_bib33","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.ab.2005.11.017","article-title":"A mediator-free phenol biosensor based on immobilizing tyrosinase to ZnO nanoparticles","volume":"349","author":"Li","year":"2006","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2018.10.025_bib34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2012\/840591","article-title":"Preparation and characterization of chitosan\/zinc oxide nanoparticles for imparting antimicrobial and UV protection to cotton fabric","volume":"2012","author":"AbdElhady","year":"2012","journal-title":"Int. J. Carbohydr. Chem."},{"key":"10.1016\/j.ab.2018.10.025_bib35","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1016\/j.bios.2007.11.019","article-title":"A novel microfluidic biosensor based on an electrical detection system for alpha-fetoprotein","volume":"23","author":"Maeng","year":"2008","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.ab.2018.10.025_bib36","first-page":"78","article-title":"Ethinylestradiol quantification in drinking water sources using a fluorescent paper based immunosensor","volume":"142","author":"Scala-Benuzzi","year":"2018","journal-title":"Microchem. J."},{"key":"10.1016\/j.ab.2018.10.025_bib37","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.aca.2003.09.018","article-title":"Novel immunoassay for Toxoplasma gondii-specific immunoglobulin G using a silica nanoparticle-based biomolecular immobilization method","volume":"501","author":"Wang","year":"2004","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.ab.2018.10.025_bib38","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1016\/S0956-5663(03)00265-3","article-title":"A piezoelectric immunoagglutination assay for Toxoplasma gondii antibodies using gold nanoparticles","volume":"19","author":"Wang","year":"2004","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.ab.2018.10.025_bib39","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.colsurfb.2012.07.021","article-title":"A novel immunosensor for detecting Toxoplasma gondii-specific IgM based on goldmag nanoparticles and graphene sheets","volume":"101","author":"Jiang","year":"2013","journal-title":"Colloids Surfaces B Biointerfaces"},{"key":"10.1016\/j.ab.2018.10.025_bib40","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.6023\/A12050257","article-title":"Disposable amperometric immunosensor for detecting Toxoplasma gondii-specific IgM based on graphene and CeO2 \u2013Au","volume":"70","author":"Jiang","year":"2012","journal-title":"Acta Chim. Sin."}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269718310947?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269718310947?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T21:25:39Z","timestamp":1757712339000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269718310947"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1]]},"references-count":40,"alternative-id":["S0003269718310947"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2018.10.025","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2019,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Serological diagnosis of Toxoplasmosis disease using a fluorescent immunosensor with chitosan-ZnO-nanoparticles","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2018.10.025","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}