{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:34:35Z","timestamp":1790620475410,"version":"4.1.0"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Shandong Provincial Natural Science Foundation, China","doi-asserted-by":"publisher","award":["ZR2016CB15"],"award-info":[{"award-number":["ZR2016CB15"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Shandong Provincial Natural Science Foundation, China","doi-asserted-by":"publisher","award":["ZR2015CM007"],"award-info":[{"award-number":["ZR2015CM007"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31501927"],"award-info":[{"award-number":["31501927"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31501928"],"award-info":[{"award-number":["31501928"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Agricultural scientific and technological innovation project of Shandong Academy of Agricultural Sciences, China","award":["CXGC2017B02"],"award-info":[{"award-number":["CXGC2017B02"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2019,3]]},"DOI":"10.1016\/j.ab.2018.12.020","type":"journal-article","created":{"date-parts":[[2018,12,27]],"date-time":"2018-12-27T11:56:44Z","timestamp":1545911804000},"page":"57-64","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":41,"special_numbering":"C","title":["A comparison of methods for effective differentiation of the frozen-thawed 3T3-L1 cells"],"prefix":"10.1016","volume":"568","author":[{"given":"Xueyan","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongmei","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liya","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanping","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiying","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2018.12.020_bib1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/0092-8674(75)90087-2","article-title":"An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion","volume":"5","author":"Green","year":"1975","journal-title":"Cell"},{"key":"10.1016\/j.ab.2018.12.020_bib2","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1073\/pnas.0137044100","article-title":"Mitotic clonal expansion: a synchronous process required for adipogenesis","volume":"100","author":"Tang","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ab.2018.12.020_bib3","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.cmet.2006.07.001","article-title":"Transcriptional control of adipocyte formation","volume":"4","author":"Farmer","year":"2006","journal-title":"Cell Metabol."},{"key":"10.1016\/j.ab.2018.12.020_bib4","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1038\/cr.2008.319","article-title":"Adipogenesis licensing and execution are disparately linked to cell proliferation","volume":"19","author":"Guo","year":"2008","journal-title":"Cell Res."},{"key":"10.1016\/j.ab.2018.12.020_bib5","doi-asserted-by":"crossref","first-page":"11988","DOI":"10.1074\/jbc.M011729200","article-title":"DNA synthesis and mitotic clonal expansion is not a required step for 3T3-L1 preadipocyte differentiation into adipocytes","volume":"276","author":"Qiu","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ab.2018.12.020_bib6","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1042\/cs0970363","article-title":"Effects of acidosis on leptin secretion from 3T3-L1 adipocytes and on serum leptin in the uraemic rat","volume":"97","author":"Teta","year":"1999","journal-title":"Clin. Sci."},{"key":"10.1016\/j.ab.2018.12.020_bib7","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1016\/j.jnutbio.2011.06.014","article-title":"Docosahexaenoic acid attenuates macrophage-induced inflammation and improves insulin sensitivity in adipocytes-specific differential effects between LC n-3 PUFA","volume":"23","author":"Oliver","year":"2012","journal-title":"JNB (J. Nutr. Biochem.)"},{"key":"10.1016\/j.ab.2018.12.020_bib8","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/j.nutres.2012.06.001","article-title":"Grape skin extract reduces adipogenesis- and lipogenesis-related gene expression in 3T3-L1 adipocytes through the peroxisome proliferator-activated receptor-\u03b3 signaling pathway","volume":"32","author":"Jeong","year":"2012","journal-title":"Nutr. Res."},{"key":"10.1016\/j.ab.2018.12.020_bib9","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.ab.2012.03.005","article-title":"Protocol for effective differentiation of 3T3-L1 cells to adipocytes","volume":"425","author":"Zebisch","year":"2012","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2018.12.020_bib10","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.ab.2016.05.007","article-title":"Prolonged treatment with 3-isobutyl-1-methylxanthine improves the efficiency of differentiating 3T3-L1 cells into adipocytes","volume":"507","author":"Hua","year":"2016","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2018.12.020_bib11","unstructured":"American Type Culture Collection, Chemically-induced Differentiation of ATCC CL-173 (3T3-L1) Using Single-component Commercially-available Reagents. http:\/\/www.atcc.org\/~\/media\/6124AF1E4C2A47CF904435117909AC25.ashx."},{"key":"10.1016\/j.ab.2018.12.020_bib12","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1258\/ebm.2010.010063","article-title":"Cell line models for differentiation: preadipocytes and adipocytes","volume":"235","author":"Poulos","year":"2010","journal-title":"Exp. Biol. Med."},{"key":"10.1016\/j.ab.2018.12.020_bib13","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1089\/scd.2011.0040","article-title":"Current methods of adipogenic differentiation of mesenchymal stem cells","volume":"20","author":"Scott","year":"2011","journal-title":"Stem Cell. Dev."},{"key":"10.1016\/j.ab.2018.12.020_bib14","doi-asserted-by":"crossref","DOI":"10.3390\/nu9080856","article-title":"Cell-surface and nuclear receptors in the colon as targets for bacterial metabolites and its relevance to colon Health","volume":"9","author":"Sivaprakasam","year":"2017","journal-title":"Nutrients"},{"key":"10.1016\/j.ab.2018.12.020_bib15","doi-asserted-by":"crossref","first-page":"2231","DOI":"10.1101\/gad.13.17.2231","article-title":"Activation and centromeric localization of CCAAT\/enhancer-binding proteins during the mitotic clonal expansion of adipocyte differentiation","volume":"13","author":"Tang","year":"1999","journal-title":"Genes Dev."},{"key":"10.1016\/j.ab.2018.12.020_bib16","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1074\/jbc.R114.619957","article-title":"Transcriptional regulation of adipocyte differentiation: a central role for CCAAT\/enhancer-binding protein (C\/EBP) \u03b2","volume":"290","author":"Guo","year":"2015","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ab.2018.12.020_bib17","doi-asserted-by":"crossref","unstructured":"P. Tontonoz, and B.M. Spiegelman, Fat and beyond: The Diverse Biology of PPAR\u03b3 - Annu. Rev. Biochem., 77(1):289.","DOI":"10.1146\/annurev.biochem.77.061307.091829"},{"key":"10.1016\/j.ab.2018.12.020_bib18","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1038\/nm.3159","article-title":"PPAR\u03b3 signaling and metabolism: the good, the bad and the future","volume":"19","author":"Ahmadian","year":"2013","journal-title":"Nat. Med."},{"key":"10.1016\/j.ab.2018.12.020_bib19","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.semcdb.2012.01.003","article-title":"PPARs: fatty acid sensors controlling metabolism","volume":"23","author":"Ll","year":"2012","journal-title":"Semin. Cell Dev. Biol."},{"key":"10.1016\/j.ab.2018.12.020_bib20","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1186\/1471-2164-12-152","article-title":"Cross species comparison of C\/EBP\u03b1 and PPAR\u03b3 profiles in mouse and human adipocytes reveals interdependent retention of binding sites","volume":"12","author":"Schmidt","year":"2011","journal-title":"BMC Genomics"},{"key":"10.1016\/j.ab.2018.12.020_bib21","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1146\/annurev.biochem.77.061307.091829","article-title":"Fat and beyond: the diverse biology of PPARgamma","volume":"77","author":"Tontonoz","year":"2008","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.ab.2018.12.020_bib22","doi-asserted-by":"crossref","first-page":"2941","DOI":"10.1101\/gad.1709008","article-title":"PPAR\u03b3 and C\/EBP factors orchestrate adipocyte biology via adjacent binding on a genome-wide scale","volume":"22","author":"Lefterova","year":"2008","journal-title":"Genes Dev."},{"key":"10.1016\/j.ab.2018.12.020_bib23","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1164\/ajrccm.157.2.9708012","article-title":"Phosphodiesterase isozymes: molecular targets for novel antiasthma agents","volume":"157","author":"Torphy","year":"1998","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"10.1016\/j.ab.2018.12.020_bib24","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1101\/gad.9.2.168","article-title":"Cascade regulation of terminal adipocyte differentiation by three members of the C\/EBP family of leucine zipper proteins","volume":"9","author":"Yeh","year":"1995","journal-title":"Genes Dev."},{"key":"10.1016\/j.ab.2018.12.020_bib25","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.bbrc.2010.07.038","article-title":"Transcriptional activation of peroxisome proliferator-activated receptor-\u03b3 requires activation of both protein kinase A and Akt during adipocyte differentiation","volume":"399","author":"Kim","year":"2010","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.ab.2018.12.020_bib26","doi-asserted-by":"crossref","first-page":"3804","DOI":"10.1128\/MCB.00709-07","article-title":"Cyclic AMP (cAMP)-Mediated stimulation of adipocyte differentiation requires the synergistic action of epac- and cAMP-dependent protein kinase-dependent processes","volume":"28","author":"Petersen","year":"2008","journal-title":"Mol. Cell Biol."},{"key":"10.1016\/j.ab.2018.12.020_bib27","doi-asserted-by":"crossref","first-page":"1700","DOI":"10.2337\/diabetes.49.10.1700","article-title":"Dexamethasone-induced insulin resistance in 3T3-L1 adipocytes is due to inhibition of glucose transport rather than insulin signal transduction","volume":"49","author":"Sakoda","year":"2000","journal-title":"Diabetes"},{"key":"10.1016\/j.ab.2018.12.020_bib28","doi-asserted-by":"crossref","first-page":"11237","DOI":"10.1016\/S0021-9258(18)99154-4","article-title":"Differentiation of 3T3-L1 preadipocytes with 3-isobutyl-1-methylxanthine and dexamethasone stimulates cell-associated and soluble chondroitin 4-sulfate proteoglycans","volume":"266","author":"Calvo","year":"1991","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ab.2018.12.020_bib29","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1074\/jbc.270.2.647","article-title":"Insulin regulates transcription of the CCAAT\/enhancer binding protein (C\/EBP) alpha, beta, and delta genes in fully-differentiated 3T3-L1 adipocytes","volume":"270","author":"Macdougald","year":"1995","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ab.2018.12.020_bib30","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.tem.2012.03.001","article-title":"Thiazolidinediones and PPAR\u03b3 agonists: time for a reassessment","volume":"23","author":"Cariou","year":"2012","journal-title":"Trends Endocrinol. Metabol."},{"key":"10.1016\/j.ab.2018.12.020_bib31","doi-asserted-by":"crossref","first-page":"1536","DOI":"10.1210\/me.2013-1080","article-title":"Acute genome-wide effects of rosiglitazone on PPAR\u03b3 transcriptional networks in adipocytes","volume":"27","author":"Haakonsson","year":"2013","journal-title":"Mol. Endocrinol."},{"key":"10.1016\/j.ab.2018.12.020_bib32","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1038\/sj.ijo.0801520","article-title":"Characterization of a human preadipocyte cell strain with high capacity for adipose differentiation","volume":"25","author":"Wabitsch","year":"2000","journal-title":"Int. J. Obes."},{"key":"10.1016\/j.ab.2018.12.020_bib33","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1159\/000145784","article-title":"Human SGBS cells \u2013 a unique tool for studies of human fat cell biology","volume":"1","author":"Fischerposovszky","year":"2008","journal-title":"Obesity Facts"}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269718309758?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269718309758?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T21:25:29Z","timestamp":1757712329000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269718309758"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3]]},"references-count":33,"alternative-id":["S0003269718309758"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2018.12.020","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2019,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A comparison of methods for effective differentiation of the frozen-thawed 3T3-L1 cells","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2018.12.020","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}