{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T03:32:12Z","timestamp":1767929532524,"version":"3.49.0"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T00:00:00Z","timestamp":1564617600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T00:00:00Z","timestamp":1564617600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2019,8]]},"DOI":"10.1016\/j.ab.2019.05.002","type":"journal-article","created":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T11:42:04Z","timestamp":1557229324000},"page":"29-35","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Real-time monitoring of D-Ala-D-Ala dipeptidase activity of VanX in living bacteria by isothermal titration calorimetry"],"prefix":"10.1016","volume":"578","author":[{"given":"Miao","family":"Lv","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue-Juan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Ge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mu-Han","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke-Wu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2019.05.002_bib1","first-page":"442","volume":"284","author":"Walsh","year":"1999","journal-title":"Deconstruct. Vancomycin, Sci."},{"key":"10.1016\/j.ab.2019.05.002_bib2","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1002\/(SICI)1521-3773(19990503)38:9<1172::AID-ANIE1172>3.0.CO;2-C","article-title":"The vancomycin group of antibiotics and the fight against resistant bacteria","volume":"38","author":"Williams","year":"1999","journal-title":"Chem. Intl. Ed."},{"key":"10.1016\/j.ab.2019.05.002_bib3","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1039\/b100912p","article-title":"Glycopeptide antibiotics and development of inhibitors to overcome vancomycin resistance","volume":"19","author":"Gao","year":"2002","journal-title":"Nat. Prod. Rep."},{"key":"10.1016\/j.ab.2019.05.002_bib4","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1111\/j.1348-0421.2007.tb03898.x","article-title":"Molecular characterization of methicillin\u2010resistant Staphylococcus aureus in hospitals in niigata, Japan: divergence and transmission","volume":"51","author":"Zaraket","year":"2007","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.ab.2019.05.002_bib5","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1126\/science.1090956","article-title":"Genetic analysis of a high-level vancomycin-resistant isolate of Staphylococcus aureus","volume":"302","author":"Weigel","year":"2003","journal-title":"Science"},{"key":"10.1016\/j.ab.2019.05.002_bib6","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1128\/jb.175.1.117-127.1993","article-title":"Characterization of Tn1546, a Tn3-related transposon conferring glycopeptide resistance by synthesis of depsipeptide peptidoglycan precursors in Enterococcus faecium BM4147","volume":"175","author":"Arthur","year":"1993","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.ab.2019.05.002_bib7","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1128\/AAC.37.8.1563","article-title":"Mechanisms of glycopeptide resistance in enterococci","volume":"37","author":"Arthur","year":"1993","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ab.2019.05.002_bib8","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S1074-5521(96)90079-4","article-title":"Bacterial resistance to vancomycin: five genes and one missing hydrogen bond tell the story","volume":"3","author":"Walsh","year":"1996","journal-title":"Chem. Biol."},{"key":"10.1016\/j.ab.2019.05.002_bib9","doi-asserted-by":"crossref","first-page":"5057","DOI":"10.1021\/bi00070a013","article-title":"Purification and characterization of VanR and the cytosolic domain of VanS: a two-component regulatory system required for vancomycin resistance in Enterococcus faecium BM4147","volume":"32","author":"Wright","year":"1993","journal-title":"Biochemistry"},{"key":"10.1016\/j.ab.2019.05.002_bib10","doi-asserted-by":"crossref","first-page":"4625","DOI":"10.1021\/bi00181a024","article-title":"Identification of the DNA-binding site for the phosphorylated VanR protein required for vancomycin resistance in Enterococcus faecium","volume":"33","author":"Holman","year":"1994","journal-title":"Biochemistry"},{"key":"10.1016\/j.ab.2019.05.002_bib11","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1111\/j.1365-2958.1994.tb00497.x","article-title":"Glycopeptide resistance mediated by enterococcal transposon Tn1546 requires production of VanX for hydrolysis of D-alanyl-D-alanine","volume":"13","author":"Reynolds","year":"1994","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.ab.2019.05.002_bib12","doi-asserted-by":"crossref","first-page":"2455","DOI":"10.1021\/bi00008a008","article-title":"Overexpression, purification, and characterization of VanX, a D-, D-dipeptidase which is essential for vancomycin resistance in Enterococcus faecium BM4147","volume":"34","author":"Wu","year":"1995","journal-title":"Biochemistry"},{"key":"10.1016\/j.ab.2019.05.002_bib13","first-page":"1785","article-title":"Dithiol Compounds:\u2009 potent, time-dependent inhibitors of VanX, a zinc-dependent d,d-dipeptidase required for vancomycin resistance in Enterococcus faecium","volume":"118","author":"Wu","year":"1996","journal-title":"J. Appl. Comput. Sci."},{"key":"10.1016\/j.ab.2019.05.002_bib14","doi-asserted-by":"crossref","first-page":"7224","DOI":"10.1016\/j.bmcl.2011.09.020","article-title":"Synthesis and activity study of phosphonamidate dipeptides as potential inhibitors of VanX","volume":"21","author":"Yang","year":"2011","journal-title":"Biorg Med Chem Lett"},{"key":"10.1016\/j.ab.2019.05.002_bib15","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0003-2697(81)90175-5","article-title":"Modified colorimetric ninhydrin methods for peptidase assay","volume":"118","author":"Doi","year":"1981","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2019.05.002_bib16","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1006\/abio.1998.2870","article-title":"Vancomycin resistance in Enterococcus faecium: a capillary electrophoresis- based assay for VanX enzyme","volume":"264","author":"Tu","year":"1998","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2019.05.002_bib17","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1034\/j.1399-3011.2003.00072.x","article-title":"New chromogenic dipeptide substrate for continuous assay of the D-alanyl-D-alanine dipeptidase VanX required for high-level vancomycin resistance","volume":"62","author":"Anissimova","year":"2003","journal-title":"J. Pept. Res."},{"key":"10.1016\/j.ab.2019.05.002_bib18","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0091-679X(07)84004-0","article-title":"Isothermal titration calorimetry: experimental design, data analysis, and probing macromolecule\/ligand binding and kinetic interactions","volume":"84","author":"Freyer","year":"2008","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.ab.2019.05.002_bib19","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1006\/abio.2001.5218","article-title":"Enzyme kinetics determined using calorimetry: a general assay for enzyme activity?","volume":"296","author":"Todd","year":"2001","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2019.05.002_bib20","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1016\/j.bbagen.2015.11.006","article-title":"Determination of affinity and efficacy of acetylcholinesterase inhibitors using isothermal titration calorimetry","volume":"1860","author":"Draczkowski","year":"2016","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ab.2019.05.002_bib21","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.ymeth.2014.12.003","article-title":"Enzyme kinetics determined by single-injection isothermal titration calorimetry","volume":"76","author":"Transtrum","year":"2015","journal-title":"Methods"},{"key":"10.1016\/j.ab.2019.05.002_bib22","doi-asserted-by":"crossref","first-page":"3120","DOI":"10.1039\/C4SC00601A","article-title":"pH and basicity of ligands control the binding of metal-ions to B. cereus B1 \u03b2-lactamase","volume":"5","author":"Motara","year":"2014","journal-title":"Chem. Sci."},{"key":"10.1016\/j.ab.2019.05.002_bib23","doi-asserted-by":"crossref","first-page":"10498","DOI":"10.1021\/bi970543u","article-title":"Mutational analysis of potential zinc-binding residues in the active site of the enterococcal D-Ala-D-Ala dipeptidase VanX","volume":"36","author":"Mccafferty","year":"1997","journal-title":"Biochemistry"},{"key":"10.1016\/j.ab.2019.05.002_bib24","series-title":"Performance Standards for Antimicrobial Susceptibility Testing: Twenty-Second Informational Supplement","author":"Cockerill","year":"2015"},{"key":"10.1016\/j.ab.2019.05.002_bib25","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1086\/513938","article-title":"Medical microbiology: antimicrobial susceptibility testing: a review of general principles and contemporary practices","volume":"26","author":"Jorgensen","year":"1998","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.ab.2019.05.002_bib26","first-page":"13050","article-title":"Probing the reaction mechanism of the d-ala-d-ala dipeptidase, VanX, by using stopped-flow kinetic and rapid-freeze quench EPR studies on the Co(II)-Substituted enzyme","volume":"128","author":"Matthews","year":"2006","journal-title":"J. Appl. Comput. Sci."},{"key":"10.1016\/j.ab.2019.05.002_bib27","doi-asserted-by":"crossref","first-page":"3507","DOI":"10.1021\/jo00065a010","article-title":"Calorimetric evaluation of enzyme kinetic parameters","volume":"58","author":"Williams","year":"1993","journal-title":"J. Org. Chem."},{"key":"10.1016\/j.ab.2019.05.002_bib28","first-page":"10508","article-title":"Direct determination of thiol pKa by isothermal titration microcalorimetry","volume":"126","author":"ajc","year":"2004","journal-title":"J. Appl. Comput. Sci."},{"key":"10.1016\/j.ab.2019.05.002_bib29","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1021\/np501025r","article-title":"Isolation, structural modification, and HIV inhibition of pentacyclic lupane-type triterpenoids from cassine xylocarpa and Maytenus cuzcoina","volume":"78","author":"Callies","year":"2015","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.ab.2019.05.002_bib30","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.bbagen.2010.10.005","article-title":"Deuteration as a tool in investigating the role of protons in cell signaling","volume":"1810","author":"Hirakura","year":"2011","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ab.2019.05.002_bib31","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1007\/s10973-015-5203-y","article-title":"Study of the growth of Enterococcus faecalis, Escherichia coli and their mixtures by microcalorimetry","volume":"125","author":"Vazquez","year":"2016","journal-title":"J. Therm. Anal. Calorim."},{"key":"10.1016\/j.ab.2019.05.002_bib32","doi-asserted-by":"crossref","first-page":"2031","DOI":"10.1016\/j.bbagen.2017.04.011","article-title":"Characterization of \u03b2-lactamase activity using isothermal titration calorimetry","volume":"1861","author":"Wang","year":"2017","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ab.2019.05.002_bib33","doi-asserted-by":"crossref","DOI":"10.1021\/acsinfecdis.8b00147","article-title":"Real-time monitoring of NDM-1 activity in live bacterial cells by isothermal titration calorimetry: a new approach to measure inhibition of antibiotic-resistant bacteria","author":"Zhang","year":"2018","journal-title":"ACS Infect. Dis."}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269718312156?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269718312156?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T21:25:55Z","timestamp":1757712355000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269718312156"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8]]},"references-count":33,"alternative-id":["S0003269718312156"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2019.05.002","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2019,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Real-time monitoring of D-Ala-D-Ala dipeptidase activity of VanX in living bacteria by isothermal titration calorimetry","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2019.05.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}