{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T12:33:59Z","timestamp":1761395639645,"version":"3.44.0"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2020,2]]},"DOI":"10.1016\/j.ab.2019.113569","type":"journal-article","created":{"date-parts":[[2019,12,27]],"date-time":"2019-12-27T19:33:04Z","timestamp":1577475184000},"page":"113569","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Generation and characterization of murine monoclonal antibodies against immunoreactive trypsinogen for newborn screening of cystic fibrosis"],"prefix":"10.1016","volume":"591","author":[{"given":"Greilys","family":"Morej\u00f3n Garc\u00eda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iria","family":"Garc\u00eda de la Rosa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadys","family":"Feal Carballo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa M.","family":"Castells Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imara C.","family":"Stable Vernier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joel M.","family":"Quintana Guerra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liliana","family":"Hern\u00e1ndez P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yesdiley","family":"Lafita Delfino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro L.","family":"P\u00e9rez Mor\u00e1s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayl\u00edn","family":"Pupo Infante","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan E.","family":"Figueredo Lago","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernesto C.","family":"Gonz\u00e1lez Reyes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2019.113569_bib1","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1097\/GIM.0b013e31818e55a2","article-title":"Clinical practice and genetic counselling for cystic fibrosis and CFTR-related disorders","volume":"10","author":"Moskowitz","year":"2008","journal-title":"Genet. Med."},{"issue":"19","key":"10.1016\/j.ab.2019.113569_bib2","doi-asserted-by":"crossref","first-page":"3476","DOI":"10.2174\/13816128113199990317","article-title":"F508del-CFTR rescue: a matter of cell stress response","volume":"19","author":"Nieddu","year":"2013","journal-title":"Curr. Pharmaceut. Des."},{"issue":"4","key":"10.1016\/j.ab.2019.113569_bib3","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.prrv.2012.05.003","article-title":"The impact of newborn screening and earlier intervention on the clinical course of cystic fibrosis","volume":"13","author":"Dijk","year":"2012","journal-title":"Paediatr. Respir. Rev."},{"key":"10.1016\/j.ab.2019.113569_bib4","doi-asserted-by":"crossref","first-page":"S33","DOI":"10.1016\/j.jpeds.2016.09.065","article-title":"Diagnosis of cystic fibrosis in screened populations","volume":"181S","author":"Farrell","year":"2017","journal-title":"J. Pediatr."},{"key":"10.1016\/j.ab.2019.113569_bib5","doi-asserted-by":"crossref","first-page":"15010","DOI":"10.1038\/nrdp.2015.10","article-title":"Cystic fibrosis","volume":"1","author":"Ratjen","year":"2015","journal-title":"Nat. Rev. Dis. Primers"},{"issue":"6 Pt 2","key":"10.1016\/j.ab.2019.113569_bib6","doi-asserted-by":"crossref","first-page":"e97","DOI":"10.1016\/j.lpm.2017.04.010","article-title":"The diagnosis of cystic fibrosis","volume":"46","author":"DeBoeck","year":"2017","journal-title":"Presse Med."},{"key":"10.1016\/j.ab.2019.113569_bib7","doi-asserted-by":"crossref","first-page":"S4","DOI":"10.1016\/j.jpeds.2016.09.064","article-title":"Diagnosis of cystic fibrosis: consensus guidelines from the Cystic Fibrosis Foundation","volume":"181S","author":"Farrell","year":"2017","journal-title":"J. Pediatr."},{"issue":"1","key":"10.1016\/j.ab.2019.113569_bib8","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.jcf.2006.05.008","article-title":"A survey of newborn screening for cystic fibrosis in Europe","volume":"6","author":"Southern","year":"2007","journal-title":"J. Cyst. Fibros."},{"issue":"3","key":"10.1016\/j.ab.2019.113569_bib9","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.anpedi.2009.06.020","article-title":"Grupo de Trabajo de Fibrosis Qu\u00edstica, Protocolo de diagn\u00f3stico y seguimiento de los pacientes con fibrosis qu\u00edstica","volume":"71","author":"Barrio G\u00f3mez de Ag\u00fcero","year":"2009","journal-title":"An. Pediatr."},{"issue":"3","key":"10.1016\/j.ab.2019.113569_bib10","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.jcf.2009.01.004","article-title":"European best practice guidelines for cystic fibrosis neonatal screening","volume":"8","author":"Castellani","year":"2009","journal-title":"J. Cyst. Fibros."},{"key":"10.1016\/j.ab.2019.113569_bib11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ymgme.2012.02.013","article-title":"Immunoreactive trypsinogen (IRT) as a biomarker for cystic fibrosis: challenges in newborn dried blood spot screening","volume":"106","author":"Therrell","year":"2012","journal-title":"Mol. Genet. Metab."},{"issue":"8","key":"10.1016\/j.ab.2019.113569_bib12","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/S2213-2600(16)00053-9","article-title":"Newborn screening for cystic fibrosis","volume":"4","author":"Castellani","year":"2016","journal-title":"Lancet Respir. Med."},{"key":"10.1016\/j.ab.2019.113569_bib13","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1186\/s13023-014-0166-9","article-title":"Differences in immunoreactive trypsin values between type of feeding and ethnicity in neonatal cystic fibrosis screening: a cross-sectional study","volume":"9","author":"Cort\u00e9s","year":"2014","journal-title":"Orphanet J. Rare Dis."},{"issue":"1","key":"10.1016\/j.ab.2019.113569_bib14","doi-asserted-by":"crossref","first-page":"14","DOI":"10.37757\/MR2022.V24.N1.12","article-title":"Generating appropriate technologies for health equity","volume":"11","author":"Reed","year":"2009","journal-title":"MEDICC Rev."},{"key":"10.1016\/j.ab.2019.113569_bib15","first-page":"1","article-title":"SUMA technology and newborn screening tests for inherited metabolic diseases in Cuba: an overview of the first 30 years","volume":"4","author":"Gonz\u00e1lez","year":"2016","journal-title":"J. Inborn Errors Metab. Screen."},{"issue":"3","key":"10.1016\/j.ab.2019.113569_bib16","article-title":"Estudio molecular de fibrosis qu\u00edstica en Cuba","volume":"5","author":"Collazo","year":"2015","journal-title":"An. Acad. Cienc. Cuba"},{"issue":"4","key":"10.1016\/j.ab.2019.113569_bib17","first-page":"535","article-title":"Rese\u00f1a hist\u00f3rica de la fibrosis qu\u00edstica y su estudio y tratamiento en Cuba","volume":"86","author":"Gonz\u00e1lez","year":"2014","journal-title":"Rev. Cuba. Pediatr."},{"key":"10.1016\/j.ab.2019.113569_bib18","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1111\/j.1567-1364.2011.00749.x","article-title":"Recombinant expression of ShPI-1A, a non-specific BPTI-Kunitz-type inhibitor, and its protection effect on proteolytic degradation of recombinant human miniproinsulin expressed in Pichia pastoris","volume":"11","author":"Gil","year":"2011","journal-title":"FEMS Yeast Res."},{"key":"10.1016\/j.ab.2019.113569_bib19","first-page":"1289","article-title":"Structure of the recombinant BPTI\/Kunitz-type inhibitor rShPI-1A from the marine invertebrate Stichodactyla helianthus","volume":"F68","author":"Garc\u00eda-Fern\u00e1ndez","year":"2012","journal-title":"Acta Crystallogr."},{"key":"10.1016\/j.ab.2019.113569_bib20","first-page":"1","article-title":"Generation and characterization of monoclonal antibodies against thyroid-stimulating hormone for newborn screening of congenital hypothyroidism","volume":"1","author":"Garc\u00eda de la Rosa","year":"2019","journal-title":"J. Immunoass. Immunochem."},{"issue":"4","key":"10.1016\/j.ab.2019.113569_bib21","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1089\/153685902760213930","article-title":"Specific monoclonal antibody against human trypsin","volume":"21","author":"Acosta","year":"2002","journal-title":"Hybrid. Hybridomics"},{"issue":"6","key":"10.1016\/j.ab.2019.113569_bib22","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1089\/153685902321044025","article-title":"Monoclonal antibody against human trypsin: production, characterization, and use for diagnosis","volume":"21","author":"Acosta","year":"2002","journal-title":"Hybrid. Hybridomics"},{"year":"1996","series-title":"Institute of Laboratory Animals Resources. Guide for the Care and Use of Laboratory Animals","key":"10.1016\/j.ab.2019.113569_bib23"},{"year":"1991","series-title":"Monoclonal Antibody and Immunosensor Technology","author":"Campbell","key":"10.1016\/j.ab.2019.113569_bib24"},{"year":"1995","series-title":"La Habana","author":"Gavilondo","key":"10.1016\/j.ab.2019.113569_bib25"},{"issue":"4","key":"10.1016\/j.ab.2019.113569_bib26","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1089\/027245700429891","article-title":"Production and characterization of specific monoclonal antibodies of the human thyroid stimulating hormone","volume":"19","author":"Baluja","year":"2000","journal-title":"Hybridoma"},{"issue":"1","key":"10.1016\/j.ab.2019.113569_bib27","first-page":"23","article-title":"Obtention of 17\u03b1-hydroxyprogesterone\/alkaline phosphatase conjugates for use in the diagnosis of congenital adrenal hyperplasia","volume":"34","author":"Marrero","year":"2003","journal-title":"Rev. CENIC (Cent. Nac. Investig. Cient.) Cienc. Biol."},{"key":"10.1016\/j.ab.2019.113569_bib28","series-title":"Immunoassay","first-page":"237","article-title":"The avidin-biotin system","author":"Bayer","year":"1996"},{"issue":"6","key":"10.1016\/j.ab.2019.113569_bib29","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1128\/jcm.29.6.1171-1174.1991","article-title":"Determination of genetic variation within Plasmodium falciparum by using enzymatically amplified DNA from filter paper disks impregnated with whole blood","volume":"29","author":"Kain","year":"1991","journal-title":"J. Clin. Microbiol."},{"year":"1994","series-title":"The Immunoassay Handbook","author":"Wild","key":"10.1016\/j.ab.2019.113569_bib30"},{"year":"2008","series-title":"Bases metodol\u00f3gicas para la evaluaci\u00f3n de anticuerpos en ensayos cl\u00ednicos de vacunas mediante t\u00e9cnicas inmunoenzim\u00e1ticas","author":"Ochoa","key":"10.1016\/j.ab.2019.113569_bib31"},{"issue":"6","key":"10.1016\/j.ab.2019.113569_bib32","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1016\/j.jpeds.2015.03.042","article-title":"New challenges in the diagnosis and management of cystic fibrosis","volume":"166","author":"Levy","year":"2015","journal-title":"J. Pediatr."},{"issue":"1","key":"10.1016\/j.ab.2019.113569_bib33","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.jcf.2008.09.004","article-title":"Common mutations in Cuban cystic fibrosis patients","volume":"8","author":"Collazo","year":"2009","journal-title":"J. Cyst. Fibros."},{"year":"1992","series-title":"Monoclonal Antibodies","author":"Peters","key":"10.1016\/j.ab.2019.113569_bib34"},{"key":"10.1016\/j.ab.2019.113569_bib35","doi-asserted-by":"crossref","first-page":"2298","DOI":"10.1073\/pnas.85.7.2298","article-title":"Identification of mutant monoclonal antibodies with increased antigen binding","volume":"85","author":"Pollock","year":"1988","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"year":"2013","series-title":"Bioqu\u00edmica M\u00e9dica","author":"Cardell\u00e1","key":"10.1016\/j.ab.2019.113569_bib36"},{"key":"10.1016\/j.ab.2019.113569_bib37","doi-asserted-by":"crossref","first-page":"136","DOI":"10.3109\/00365511003615317","article-title":"Human trypsinogens in the pancreas and in cancer","volume":"70","author":"Itkonen","year":"2010","journal-title":"Scand. J. Clin. Lab. Investig."},{"issue":"2","key":"10.1016\/j.ab.2019.113569_bib38","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1258\/096914106777589623","article-title":"Development and characterization of dried blood spot materials for the measurement of immunoreactive trypsinogen","volume":"13","author":"Li","year":"2006","journal-title":"J. Med. Screen"},{"issue":"4","key":"10.1016\/j.ab.2019.113569_bib39","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1590\/S0102-311X2008001600002","article-title":"Cystic fibrosis and neonatal screening","volume":"24","author":"Rodrigues","year":"2008","journal-title":"Cad. Sa\u00fade P\u00fablica"}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269719309753?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269719309753?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T21:29:21Z","timestamp":1757712561000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269719309753"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2]]},"references-count":39,"alternative-id":["S0003269719309753"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2019.113569","relation":{},"ISSN":["0003-2697"],"issn-type":[{"type":"print","value":"0003-2697"}],"subject":[],"published":{"date-parts":[[2020,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Generation and characterization of murine monoclonal antibodies against immunoreactive trypsinogen for newborn screening of cystic fibrosis","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2019.113569","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"113569"}}