{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T15:38:34Z","timestamp":1777477114090,"version":"3.51.4"},"reference-count":32,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T00:00:00Z","timestamp":1619827200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T00:00:00Z","timestamp":1619827200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T00:00:00Z","timestamp":1619827200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T00:00:00Z","timestamp":1619827200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T00:00:00Z","timestamp":1619827200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T00:00:00Z","timestamp":1619827200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T00:00:00Z","timestamp":1619827200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["16-16-04108"],"award-info":[{"award-number":["16-16-04108"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2021,5]]},"DOI":"10.1016\/j.ab.2021.114113","type":"journal-article","created":{"date-parts":[[2021,1,30]],"date-time":"2021-01-30T06:31:53Z","timestamp":1611988313000},"page":"114113","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"special_numbering":"C","title":["Environmentally friendly method of RNA isolation"],"prefix":"10.1016","volume":"620","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0245-0641","authenticated-orcid":false,"given":"Yuri F.","family":"Drygin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konstantin O.","family":"Butenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatiana V.","family":"Gasanova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2021.114113_bib1","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.1042\/bj0321707","article-title":"The disintegration of tobacco mosaic virus preparations with sodium dodecyl sulphate","volume":"32","author":"Sreenivasaya","year":"1938","journal-title":"Biochem. J."},{"key":"10.1016\/j.ab.2021.114113_bib2","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1042\/bj0640405","article-title":"A new method for the isolation of ribonucleic acids from mammalian tissues","volume":"64","author":"Kirby","year":"1956","journal-title":"Biochem. J."},{"key":"10.1016\/j.ab.2021.114113_bib3","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1042\/bj1350559","article-title":"The use of sodium perchlorate in deproteinization during the preparation of nucleic acids","volume":"135","author":"Wilcockson","year":"1973","journal-title":"Biochem. J."},{"key":"10.1016\/j.ab.2021.114113_bib4","doi-asserted-by":"crossref","first-page":"4791","DOI":"10.1093\/nar\/26.21.4791","article-title":"Natural covalent complexes of nucleic acids and proteins: some comments on practice and theory on the path from well-known complexes to new ones","volume":"26","author":"Drygin","year":"1998","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ab.2021.114113_bib5","series-title":"Molecular Cloning a Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"10.1016\/j.ab.2021.114113_bib6","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/0003-2697(87)90021-2","article-title":"Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction","volume":"162","author":"Chomczynski","year":"1987","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2021.114113_bib7","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1128\/jcm.28.3.495-503.1990","article-title":"Rapid and simple method for purification of nucleic acids","volume":"28","author":"Boom","year":"1990","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ab.2021.114113_bib8","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/0005-2787(66)90326-1","article-title":"The chromatographic separation of ribonucleic acids","volume":"114","author":"Barber","year":"1966","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.ab.2021.114113_bib9","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.1073\/pnas.55.6.1504","article-title":"Purification and properties of the replicative intermediate of the RNA bacteriophage R17","volume":"55","author":"Franklin","year":"1966","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.ab.2021.114113_bib10","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1042\/bj1350559","article-title":"The use of sodium perchlorate in deproteinization during the preparation of nucleic acids","volume":"135","author":"Wilcockson","year":"1973","journal-title":"Biochem. J."},{"key":"10.1016\/j.ab.2021.114113_bib11","doi-asserted-by":"crossref","first-page":"I07","DOI":"10.1099\/0022-1317-23-1-107","article-title":"The rapid isolation of plant virus RNAs using sodium perchlorate","volume":"23","author":"Wilcockson","year":"1974","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.ab.2021.114113_bib12","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1186\/s12864-020-6673-2","article-title":"Comparison of RNA isolation methods on RNA-Seq: implications for differential expression and meta-analyses","volume":"21","author":"Scholes","year":"2020","journal-title":"BMC Genom."},{"key":"10.1016\/j.ab.2021.114113_bib13","article-title":"A method for extracting highquality total RNA from plant rich in polysaccharides and polyphenols using Dendrobium huoshanense","volume":"13","author":"Liu","year":"2018","journal-title":"PloS One"},{"key":"10.1016\/j.ab.2021.114113_bib14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/plants6010001","article-title":"A high-throughput RNA extraction for sprouted single-seed barley (Hordeum vulgare L.) rich in polysaccharides","volume":"6","author":"Rashid","year":"2016","journal-title":"Plants"},{"key":"10.1016\/j.ab.2021.114113_bib15","first-page":"11","article-title":"RNA isolation with low toxic ammonium trichloroacetate","volume":"4","author":"Kondakova","year":"2016","journal-title":"Eur. J. Biotech. Biosci."},{"key":"10.1016\/j.ab.2021.114113_bib16","series-title":"Molecular Biology of the Cell","author":"Alberts","year":"2014"},{"key":"10.1016\/j.ab.2021.114113_bib17","first-page":"224","article-title":"Complexes formed via bioconjugation of genetically modified TMV particles with conserved influenza antigen: synthesis and characterization Biochemistry","volume":"85","author":"Gasanova","year":"2020","journal-title":"Moscow Times"},{"key":"10.1016\/j.ab.2021.114113_bib18","doi-asserted-by":"crossref","first-page":"5227","DOI":"10.1021\/jf053137n","article-title":"Isolation of high-quality RNA from apple (Malus domestica) fruit","volume":"54","author":"Asif","year":"2006","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.ab.2021.114113_bib19","article-title":"A simple and rapid method for RNA isolation from plant tissues with high phenolic compounds and polysaccharides","volume":"184","author":"Chan","year":"2007","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ab.2021.114113_bib20","article-title":"Isolation of high-quality total RNA from Chinese fir (Cunninghamia lanceolata (Lamb.) Hook)","volume":"10","author":"Ma","year":"2015","journal-title":"PloS One"},{"key":"10.1016\/j.ab.2021.114113_bib21","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1111\/tpj.13600","article-title":"Isolation of tissues and preservation of RNA from intact, germinated barley grain","volume":"91","author":"Betts","year":"2017","journal-title":"Plant J."},{"key":"10.1016\/j.ab.2021.114113_bib22","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1007\/s13205-017-1003-3","article-title":"Comparative assessment of four RNA extraction methods and modification to obtain high-quality RNA from Parthenium hysterophorus leaf","volume":"7","author":"Ahmad","year":"2017","journal-title":"3 Biotech."},{"key":"10.1016\/j.ab.2021.114113_bib23","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1042\/bj1230235","article-title":"The molecular integrity of chloroplast ribosomal ribonucleic acid","volume":"123","author":"Leaver","year":"1971","journal-title":"Biochem. J."},{"key":"10.1016\/j.ab.2021.114113_bib24","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/BF00364820","article-title":"A study of mitochondrial ribosomes from the higher plant Solanum tuberosum","volume":"11","author":"Pinel","year":"1986","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.ab.2021.114113_bib25","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1093\/chemse\/7.3-4.215","article-title":"Measurement of human olfactory thresholds for several groups of structurally related compounds","volume":"7","author":"Punter","year":"1983","journal-title":"Chem. Senses"},{"key":"10.1016\/j.ab.2021.114113_bib26","series-title":"Toxicological Chemistry","author":"Kramarenko","year":"1989"},{"key":"10.1016\/j.ab.2021.114113_bib27","series-title":"Data for Biochemical Research","author":"Dawson","year":"1986"},{"key":"10.1016\/j.ab.2021.114113_bib28","author":"PAN Pesticides database"},{"key":"10.1016\/j.ab.2021.114113_bib29","volume":"106","author":"IARC Monographs","year":"2014","journal-title":"Trichloroacetic acid"},{"key":"10.1016\/j.ab.2021.114113_bib30","series-title":"Nucleases","author":"Shapot","year":"1968"},{"key":"10.1016\/j.ab.2021.114113_bib31","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/0042-6822(61)90131-3","article-title":"Purification of viral RNA by means of bentonite","volume":"14","author":"Fraenkel-Conrat","year":"1961","journal-title":"Virology"},{"key":"10.1016\/j.ab.2021.114113_bib32","first-page":"880","article-title":"Characterization of chromosomal messenger RNA","volume":"30","author":"Samarina","year":"1965","journal-title":"Biokhimia"}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269721000142?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269721000142?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T13:57:45Z","timestamp":1777384665000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269721000142"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5]]},"references-count":32,"alternative-id":["S0003269721000142"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2021.114113","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2021,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Environmentally friendly method of RNA isolation","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2021.114113","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"114113"}}