{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T17:33:52Z","timestamp":1785000832985,"version":"3.55.0"},"reference-count":28,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T00:00:00Z","timestamp":1648080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004167","name":"Medicines for Malaria Venture","doi-asserted-by":"publisher","award":["RD\/17\/0047"],"award-info":[{"award-number":["RD\/17\/0047"]}],"id":[{"id":"10.13039\/501100004167","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011858","name":"African Academy of Sciences","doi-asserted-by":"publisher","award":["GCA\/DD\/rnd3\/006"],"award-info":[{"award-number":["GCA\/DD\/rnd3\/006"]}],"id":[{"id":"10.13039\/501100011858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2022,7]]},"DOI":"10.1016\/j.ab.2022.114669","type":"journal-article","created":{"date-parts":[[2022,3,20]],"date-time":"2022-03-20T02:10:16Z","timestamp":1647742216000},"page":"114669","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"special_numbering":"C","title":["Implementation and continued validation of the malaria Plasmodium falciparum lactate dehydrogenase-based colorimetric assay for use in antiplasmodial drug screening"],"prefix":"10.1016","volume":"648","author":[{"given":"Patrick Valere Tsouh","family":"Fokou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brice Mariscal Tchatat","family":"Tali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darline","family":"Dize","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cedric Derick Jiatsa","family":"Mbouna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cyrille Armel Njampa","family":"Ngansop","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigue","family":"Keumoe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauve Rachel Yamthe","family":"Tchokouaha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean Claude","family":"Tchouankeu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9177-9755","authenticated-orcid":false,"given":"Fanny","family":"Escudie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Duffy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3147-364X","authenticated-orcid":false,"given":"Fabrice Fekam","family":"Boyom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2022.114669_bib1","doi-asserted-by":"crossref","first-page":"661","DOI":"10.4269\/ajtmh.1968.17.661","article-title":"Effects of chloroquine, quinine, and cycloguanil upon the maturation of asexual erythrocytic forms of two strains of Plasmodium falciparum in vitro","volume":"17","author":"Rieckmann","year":"1968","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.ab.2022.114669_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fphar.2017.00754","article-title":"Development in assay methods for in vitro antimalarial drug efficacy testing : a Systematic Review","volume":"8","author":"Sinha","year":"2017","journal-title":"Front. Pharmacol."},{"issue":"6","key":"10.1016\/j.ab.2022.114669_bib3","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1038\/nrd1416","article-title":"Antimalarial drug discovery : efficacy models for compound screening","volume":"3","author":"Fidock","year":"2004","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.ab.2022.114669_bib4","doi-asserted-by":"crossref","DOI":"10.1186\/s40580-020-00223-w","article-title":"Simple , rapid , and accurate malaria diagnostic platform using microfluidic - based immunoassay of Plasmodium falciparum lactate dehydrogenase","author":"Lee","year":"2020","journal-title":"Nano Converg"},{"key":"10.1016\/j.ab.2022.114669_bib5","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0021237","article-title":"Antimalarial activity of potential inhibitors of Plasmodium falciparum lactate dehydrogenase enzyme selected by docking studies","volume":"6","author":"Penna-coutinho","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.ab.2022.114669_bib6","doi-asserted-by":"crossref","first-page":"205","DOI":"10.4269\/ajtmh.1993.48.205","article-title":"Measurement of the lactate dehydrogenase activity of Plasmodium falciparum as an assessment of parasitemia","volume":"48","author":"Makler","year":"1993","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.ab.2022.114669_bib7","doi-asserted-by":"crossref","first-page":"739","DOI":"10.4269\/ajtmh.1993.48.739","article-title":"Parasite lactate dehydrogenase as an assay for Plasmodium falciparum drug sensitivity","volume":"48","author":"Makler","year":"1993","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"1","key":"10.1016\/j.ab.2022.114669_bib8","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S0166-6851(97)00140-0","article-title":"Substrate and cofactor specificity and selective inhibition of lactate dehydrogenase from the malarial parasite P . falciparum","volume":"90","author":"Gomez","year":"1997","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.ab.2022.114669_bib9","doi-asserted-by":"crossref","first-page":"912","DOI":"10.1038\/nsb1196-912","article-title":"The structure of lactate dehydrogenase from Plasmodium falciparum reveals a new target for anti-malarial design","volume":"3","author":"Dunn","year":"1996","journal-title":"Nat. Struct. Biol."},{"issue":"6","key":"10.1016\/j.ab.2022.114669_bib10","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1084\/jem.114.6.1049","article-title":"Molecular heterogeneity of lactic dehydrogenase in avian malaria (Plasmodium lophurae)","volume":"114","author":"Sherman","year":"1961","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.ab.2022.114669_bib11","doi-asserted-by":"crossref","first-page":"6219","DOI":"10.1021\/bi049892w","article-title":"Comparative structural analysis and kinetic properties of lactate dehydrogenases from the four species of human malarial parasites","author":"Brown","year":"2004","journal-title":"Biochemistry"},{"key":"10.1016\/j.ab.2022.114669_bib12","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1126\/science.781840","article-title":"Human malaria parasites in continuous culture","volume":"193","author":"William","year":"1976","journal-title":"Science (80-.)"},{"key":"10.1016\/j.ab.2022.114669_bib13","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1128\/AAC.01512-09","article-title":"Antimalarial asexual stage-specific and gametocytocidal activities of HIV protease inhibitors","volume":"54","author":"Peatey","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ab.2022.114669_bib14","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1128\/AAC.48.5.1803-1806.2004","article-title":"Simple and inexpensive fluorescence-based technique for high-throughput antimalarial drug screening","volume":"48","author":"Smilkstein","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ab.2022.114669_bib15","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1128\/AAC.45.3.949-951.2001","article-title":"Comparison of efficacies of cysteine protease inhibitors against five strains of Plasmodium falciparum","volume":"45","author":"Singh","year":"2001","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.ab.2022.114669_bib16","doi-asserted-by":"crossref","DOI":"10.1177\/108705719900400206","article-title":"A Simple Statistical Parameter for Use in Evaluation and validation of high throughput screening assays","author":"Zhang","year":"1999","journal-title":"J. Biomol. Screen"},{"key":"10.1016\/j.ab.2022.114669_bib17","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1177\/108705719900400206","article-title":"A simple statistical parameter for use in evaluation and validation of high throughput screening assays","volume":"4","author":"Zhang","year":"1999","journal-title":"J. Biomol. Screen"},{"issue":"6","key":"10.1016\/j.ab.2022.114669_bib18","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/1475-2875-11-34","article-title":"anti-malarial drugs : how effective are they against Plasmodium falciparum gametocytes","volume":"11","author":"Peatey","year":"2012","journal-title":"Malar. J."},{"issue":"5","key":"10.1016\/j.ab.2022.114669_bib19","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/0035-9203(71)90036-8","article-title":"Evidence for cyclic development and short-lived maturity in the gametocytes of Plasmodium falciparum","volume":"65","author":"Hawking","year":"1971","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.ab.2022.114669_bib20","first-page":"49","article-title":"Limit of Blank , Limit of detection and limit of quantitation","volume":"29","author":"Armbruster","year":"2008","journal-title":"Clin. Biochem. Rev."},{"key":"10.1016\/j.ab.2022.114669_bib21","series-title":"HTS Assay Validation 2. Stability and Process Studies","first-page":"1","author":"Iversen","year":"2012"},{"key":"10.1016\/j.ab.2022.114669_bib22","first-page":"164","article-title":"Writing performance relative to writing apprehension, self-efficacy in writing, and attitudes towards writing: a correlational study in Turkish tertiary-level EFL","volume":"13","author":"Erkan","year":"2011","journal-title":"Asian EFL J."},{"key":"10.1016\/j.ab.2022.114669_bib23","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.2174\/138161211797416057","article-title":"Antimalarial peptides : the long and the short of it","author":"Bell","year":"2011","journal-title":"Curr. Pharmaceut. Des."},{"key":"10.1016\/j.ab.2022.114669_bib24","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.4155\/fmc.13.113","article-title":"Hemozoin and antimalarial drug discovery","volume":"5","author":"Fong","year":"2013","journal-title":"Future Med. Chem."},{"issue":"47","key":"10.1016\/j.ab.2022.114669_bib25","first-page":"273","article-title":"Cell Biology and Metabolism : a common mechanism for blockade of heme polymerization by antimalarial quinolines a common mechanism for blockade of heme polymerization by antimalarial quinolines","volume":"20","author":"Sullivan","year":"1998","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ab.2022.114669_bib26","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1517\/13543776.2016.1113258","article-title":"Antiplasmodial drug targets: a patent review (2000 - 2013)","volume":"26","author":"Okombo","year":"2016","journal-title":"Expert Opin. Ther. Pat."},{"key":"10.1016\/j.ab.2022.114669_bib27","doi-asserted-by":"crossref","first-page":"5","DOI":"10.4103\/0253-7613.19846","article-title":"Screening of antimalarial drugs: an overview","volume":"38","author":"Kalra","year":"2006","journal-title":"Indian J. Pharmacol."},{"key":"10.1016\/j.ab.2022.114669_bib28","doi-asserted-by":"crossref","first-page":"1926","DOI":"10.1128\/AAC.01607-06","article-title":"Assessment and continued validation of the malaria SYBR Green I-based fluorescence assay for use in malaria drug screening","volume":"51","author":"Johnson","year":"2007","journal-title":"Antimicrob. Agents Chemother."}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269722001257?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269722001257?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T11:59:29Z","timestamp":1762084769000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269722001257"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7]]},"references-count":28,"alternative-id":["S0003269722001257"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2022.114669","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2022,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Implementation and continued validation of the malaria Plasmodium falciparum lactate dehydrogenase-based colorimetric assay for use in antiplasmodial drug screening","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2022.114669","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"114669"}}