{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T17:50:54Z","timestamp":1792518654062,"version":"4.5.0"},"reference-count":53,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T00:00:00Z","timestamp":1679443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004054","name":"King Abdulaziz University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007421","name":"University of the Philippines","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007421","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NR-51683"],"award-info":[{"award-number":["NR-51683"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007512","name":"Universitat Rovira i Virgili","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007512","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010892","name":"Department of Science and Technology, Republic of the Philippines","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010892","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2023,5]]},"DOI":"10.1016\/j.ab.2023.115118","type":"journal-article","created":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T03:19:40Z","timestamp":1679455180000},"page":"115118","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Selection of G-rich ssDNA aptamers for the detection of enterotoxins of the cholera toxin family"],"prefix":"10.1016","volume":"669","author":[{"given":"Nerissa A.","family":"Molejon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine M.","family":"Lapada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasso","family":"Skouridou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Analiza P.","family":"Rollon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed S.","family":"El-Shahawi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulaziz S.","family":"Bashammakh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ciara K.","family":"O'Sullivan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2023.115118_bib1","doi-asserted-by":"crossref","first-page":"42","DOI":"10.4081\/jphr.2013.e9","article-title":"Prevalence of gastrointestinal pathogens in developed and developing countries: systematic review and meta-analysis","volume":"2","author":"Fletcher","year":"2013","journal-title":"J. Public Health Res."},{"key":"10.1016\/j.ab.2023.115118_bib2","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.tifs.2018.09.019","article-title":"Microbial food safety in the 21st century: emerging challenges and foodborne pathogenic bacteria","volume":"81","author":"P Franz","year":"2018","journal-title":"Trends Food Sci. Technol."},{"issue":"12","key":"10.1016\/j.ab.2023.115118_bib3","article-title":"World Health Organization estimates of the global and regional disease burden of 22 foodborne bacterial, protozoal, and viral diseases, 2010: a data synthesis","volume":"12","author":"Kirk","year":"2015","journal-title":"PLoS Med."},{"key":"10.1016\/j.ab.2023.115118_bib4","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.pop.2018.05.008","article-title":"Common gastrointestinal infections, Prim. Care","volume":"45","author":"Sell","year":"2018","journal-title":"Clin. Office Pract."},{"key":"10.1016\/j.ab.2023.115118_bib5","series-title":"The Comprehensive Sourcebook of Bacterial Protein Toxins","first-page":"195","article-title":"Vibrio cholerae and Escherichia coli heat-labile enterotoxins and beyond","author":"Heggelund","year":"2015"},{"key":"10.1016\/j.ab.2023.115118_bib6","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.clinmicnews.2020.09.003","article-title":"Clinically important toxins in bacterial infection: utility of laboratory detection","volume":"42","author":"Forbes","year":"2020","journal-title":"Clin. Microbiol. Newsl."},{"key":"10.1016\/j.ab.2023.115118_bib7","doi-asserted-by":"crossref","first-page":"2025","DOI":"10.1126\/science.274.5295.2025","article-title":"Global climate and infectious disease: the cholera paradigm","volume":"274","author":"Colwell","year":"1996","journal-title":"Science"},{"key":"10.1016\/j.ab.2023.115118_bib8","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1016\/S0140-6736(22)00330-0","volume":"399","author":"Kanungo","year":"2022","journal-title":"Cholera, Lancet"},{"key":"10.1016\/j.ab.2023.115118_bib10","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0003832","article-title":"Updated global burden of cholera in endemic countries","volume":"9","author":"Ali","year":"2015","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.ab.2023.115118_bib11","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1111\/1462-2920.13106","article-title":"The different ecological niches of enterotoxigenic Escherichia coli","volume":"18","author":"Gonzalez-Siles","year":"2016","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.ab.2023.115118_bib12","doi-asserted-by":"crossref","first-page":"292","DOI":"10.3389\/fcimb.2019.00292","article-title":"Review of newly identified functions associated with the heat-labile toxin of enterotoxigenic Escherichia coli","volume":"9","author":"Duan","year":"2019","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.ab.2023.115118_bib13","doi-asserted-by":"crossref","first-page":"62","DOI":"10.3390\/toxins14010062","article-title":"AB5 enteroxotin-mediated pathogenesis: perspectives gleaned from Shiga toxins","volume":"14","author":"Biernbaum","year":"2022","journal-title":"Toxins"},{"key":"10.1016\/j.ab.2023.115118_bib14","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1006\/jmbi.1995.0456","article-title":"The three-dimensional crystal structure of cholera toxin","volume":"251","author":"Zhang","year":"1995","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.ab.2023.115118_bib15","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1128\/CMR.8.1.48","volume":"8","author":"Kaper","year":"1995","journal-title":"Cholera, Clin. Microbiol. Rev."},{"key":"10.1016\/j.ab.2023.115118_bib16","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.trac.2016.01.017","article-title":"Vibrio cholerae detection: traditional assays, novel diagnostic techniques and biosensors","volume":"79","author":"Cecchini","year":"2016","journal-title":"TrAC - Trends Anal. Chem."},{"key":"10.1016\/j.ab.2023.115118_bib17","series-title":"WHO Preferred Product Characteristics for Vaccines against Enterotoxigenic Escherichia coli (ETEC)","author":"World Health Organization","year":"2021"},{"key":"10.1016\/j.ab.2023.115118_bib18","article-title":"A review of analytical methods for the detection of bacterial toxins","volume":"84","author":"Pimbley","year":"1988","journal-title":"J. Appl. Microbiol. Symp. Suppl."},{"key":"10.1016\/j.ab.2023.115118_bib20","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.mimet.2005.07.017","article-title":"Multiplex real-time PCR detection of Vibrio cholerae","volume":"65","author":"Gubala","year":"2006","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.ab.2023.115118_bib21","doi-asserted-by":"crossref","first-page":"351","DOI":"10.3389\/fcimb.2020.00351","article-title":"High-throughput multiparallel enteropathogen detection via nano-liter qPCR","volume":"10","author":"Grembi","year":"2020","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.ab.2023.115118_bib22","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1128\/JCM.00316-09","article-title":"Development of multiplex PCR assays for detection of enterotoxigenic Escherichia coli colonization factors and toxins","volume":"47","author":"Rodas","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ab.2023.115118_bib23","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1186\/1471-2180-8-94","article-title":"Sensitive and rapid detection of cholera toxin-producing Vibrio cholerae using a loop-mediated isothermal amplification","volume":"8","author":"Yamazaki","year":"2008","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.ab.2023.115118_bib24","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2019.02899","article-title":"Simple visualized detection method of virulence-associated genes of Vibrio cholerae by loop-mediated isothermal amplification","volume":"10","author":"Xu","year":"2019","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.ab.2023.115118_bib25","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/j.mimet.2006.09.024","article-title":"Rapid and sensitive detection of heat-labile I and heat-stable I enterotoxin genes of enterotoxigenic Escherichia coli by loop-mediated isothermal amplification","volume":"68","author":"Yano","year":"2007","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.ab.2023.115118_bib26","first-page":"1","article-title":"Detection of cholera toxin with surface plasmon field-enhanced fluorescent spectroscopy","author":"Seherler","year":"2021","journal-title":"Biotechnol. Appl. Biochem."},{"key":"10.1016\/j.ab.2023.115118_bib27","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.bios.2015.03.048","article-title":"Enzymatic electrochemical detection of epidemic-causing Vibrio cholerae with a disposable oligonucleotide-modified screen-printed bisensor coupled to a dry-reagent-based nucleic acid amplification assay","volume":"70","author":"Yu","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.ab.2023.115118_bib28","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1021\/ac990406y","article-title":"Amperometric detection of Escherichia coli heat-labile enterotoxin by redox diacetylenic vesciles on a sol\u2013gel thin-film electrode","volume":"72","author":"Peng","year":"2000","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ab.2023.115118_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.bioelechem.2021.107887","article-title":"A novel label-free electrochemical immunesensor for ultrasensitive detection of LT toxin using prussian blue@gold nanoparticles composite as a signal amplification","volume":"142","author":"Habibi","year":"2021","journal-title":"Bioelectrochemistry"},{"issue":"Suppl 1","key":"10.1016\/j.ab.2023.115118_bib30","doi-asserted-by":"crossref","first-page":"A73","DOI":"10.1016\/j.vaccine.2019.07.099","article-title":"Diagnostic techniques for rapid detection of Vibrio cholerae O1\/O139","volume":"38","author":"Ramamurthy","year":"2020","journal-title":"Vaccine"},{"key":"10.1016\/j.ab.2023.115118_bib31","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.mimet.2017.11.020","article-title":"Large-scale evaluation of a rapid diagnostic test for diarrhea caused by enterotoxigenic Escherichia coli targeting the heat-labile toxin","volume":"144","author":"Henrique","year":"2018","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.ab.2023.115118_bib33","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.cmi.2021.08.027","article-title":"Accuracy of cholera rapid diagnostic tests: a systematic review and meta-analysis","volume":"28","author":"Muzembo","year":"2022","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.ab.2023.115118_bib34","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1126\/science.2200121","article-title":"Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase","volume":"249","author":"Tuerk","year":"1990","journal-title":"Science"},{"key":"10.1016\/j.ab.2023.115118_bib35","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1038\/346818a0","article-title":"In vitro selection of RNA molecules that bind specific ligands","volume":"346","author":"Ellington","year":"1990","journal-title":"Nature"},{"key":"10.1016\/j.ab.2023.115118_bib36","doi-asserted-by":"crossref","first-page":"9329","DOI":"10.1021\/acsami.0c14788","article-title":"Recent developments in aptasensors for diagnostic applications","volume":"13","author":"Liu","year":"2021","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.ab.2023.115118_bib37","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1146\/annurev-bioeng-082020-035644","article-title":"Recent advances in aptamer-based biosensors for global health applications","volume":"23","author":"Stanciu","year":"2021","journal-title":"Annu. Rev. Biomed. Eng."},{"key":"10.1016\/j.ab.2023.115118_bib38","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1016\/j.talanta.2019.02.066","article-title":"Recent developments of aptasensors expedient for point-of-care (POC) diagnostics","volume":"199","author":"Citartan","year":"2019","journal-title":"Talanta"},{"key":"10.1016\/j.ab.2023.115118_bib39","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.3389\/fmicb.2016.01426","article-title":"Aptamer-based technologies in foodborne pathogen detection","volume":"7","author":"Teng","year":"2016","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.ab.2023.115118_bib40","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1111\/jam.13650","article-title":"Current advances in aptamer-assisted technologies for detecting bacterial and fungal toxins","volume":"124","author":"Alizadeh","year":"2017","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.ab.2023.115118_bib41","doi-asserted-by":"crossref","first-page":"178","DOI":"10.2144\/02321dd04","article-title":"Use of magnetic beads in selection and detection of biotoxin aptamers by electrochemiluminescence and enzymatic methods","volume":"21","author":"Bruno","year":"2002","journal-title":"Biotechniques"},{"key":"10.1016\/j.ab.2023.115118_bib42","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.jbiotec.2018.01.012","article-title":"Highly affine and selective aptamers against cholera toxin as capture elements in magnetic bead-based sandwich ELAA","volume":"269","author":"Frohnmeyer","year":"2018","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.ab.2023.115118_bib43","doi-asserted-by":"crossref","first-page":"20188","DOI":"10.1021\/acsomega.9b02412","article-title":"One-pot SELEX: identification of specific aptamers against diverse steroid targets in one selection","volume":"4","author":"Jauset-Rubio","year":"2019","journal-title":"ACS Omega"},{"key":"10.1016\/j.ab.2023.115118_bib44","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/j.omtn.2018.04.006","article-title":"AptaSUITE: a full-featured bioinformatics framework for the comprehensive analysis of aptamers from HT-SELEX experiments","volume":"11","author":"Hoinka","year":"2018","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"10.1016\/j.ab.2023.115118_bib45","doi-asserted-by":"crossref","first-page":"14810","DOI":"10.1021\/acs.analchem.1c03671","article-title":"Hybrid antibody-aptamer assay for detection of tetrodotoxin in pufferfish","volume":"93","author":"Shkembi","year":"2021","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ab.2023.115118_bib46","doi-asserted-by":"crossref","first-page":"1840","DOI":"10.1039\/C8AN01616J","article-title":"Aptamer lateral flow assays for rapid and sensitive detection of cholera toxin","volume":"144","author":"Frohnmeyer","year":"2019","journal-title":"Analyst"},{"key":"10.1016\/j.ab.2023.115118_bib47","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1093\/nar\/gkp026","article-title":"Circular dichroism and conformational polymorphism of DNA","volume":"37","author":"Kypr","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ab.2023.115118_bib48","doi-asserted-by":"crossref","first-page":"7104","DOI":"10.1021\/acs.analchem.9b00075","article-title":"High affinity aptamer for the detection of the biogenic amine histamine","volume":"91","author":"Mairal Lerga","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ab.2023.115118_bib49","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1089\/154545703322860825","article-title":"Analysis of thermal melting curves","volume":"13","author":"Mergny","year":"2003","journal-title":"Oligonucleotides"},{"key":"10.1016\/j.ab.2023.115118_bib50","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1128\/jcm.30.7.1783-1786.1992","article-title":"Evaluation of the bead enzyme-linked immunosorbent assay for detection of cholera toxin directly from stool specimens","volume":"30","author":"Ramamurthy","year":"1992","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ab.2023.115118_bib51","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1128\/iai.29.1.108-113.1980","article-title":"Detection of Escherichia coli enterotoxins in stool","volume":"29","author":"Merson","year":"1980","journal-title":"Infect. Immun."},{"key":"10.1016\/j.ab.2023.115118_bib52","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1007\/s00216-014-7947-9","article-title":"Microfluidic biosensor for cholera toxin detection in fecal samples","volume":"407","author":"Bunyakul","year":"2015","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.ab.2023.115118_bib53","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.mimet.2016.12.007","article-title":"Immunochromatographic detection of the heat-labile enterotoxin of enterotoxigenic Escherichia coli with cross-detection of cholera toxin","volume":"132","author":"Arimitsu","year":"2017","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.ab.2023.115118_bib54","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.febslet.2004.04.086","article-title":"BOF: a novel family of bacterial OB-fold proteins","volume":"567","author":"Ginalski","year":"2004","journal-title":"FEBS Lett."},{"key":"10.1016\/j.ab.2023.115118_bib55","doi-asserted-by":"crossref","first-page":"648","DOI":"10.3390\/biom12050648","article-title":"G-quadruplex-binding proteins: promising targets for drug design","volume":"12","author":"Shu","year":"2022","journal-title":"Biomolecules"},{"key":"10.1016\/j.ab.2023.115118_bib56","doi-asserted-by":"crossref","first-page":"3781","DOI":"10.3390\/molecules24203781","article-title":"G-quadruplex-forming aptamers\u2013characteristics, applications, and perspectives","volume":"24","author":"Roxo","year":"2019","journal-title":"Molecules"}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269723000830?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269723000830?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T12:00:35Z","timestamp":1762084835000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269723000830"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5]]},"references-count":53,"alternative-id":["S0003269723000830"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2023.115118","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2023,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Selection of G-rich ssDNA aptamers for the detection of enterotoxins of the cholera toxin family","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2023.115118","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"115118"}}