{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T02:04:39Z","timestamp":1768010679156,"version":"3.49.0"},"reference-count":25,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,5,24]],"date-time":"2024-05-24T00:00:00Z","timestamp":1716508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003052","name":"Korea Ministry of Trade Industry and Energy","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003706","name":"Korea Research Institute of Standards and Science","doi-asserted-by":"publisher","award":["KRISS \\u2013 2022 \\u2013 GP2023-0003"],"award-info":[{"award-number":["KRISS \\u2013 2022 \\u2013 GP2023-0003"]}],"id":[{"id":"10.13039\/501100003706","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2024,9]]},"DOI":"10.1016\/j.ab.2024.115576","type":"journal-article","created":{"date-parts":[[2024,5,23]],"date-time":"2024-05-23T23:36:24Z","timestamp":1716507384000},"page":"115576","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Validation of one-step reverse transcription digital PCR assays for Norovirus GI"],"prefix":"10.1016","volume":"692","author":[{"given":"Bomin","family":"Ko","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taejin","family":"Shin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boram","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0580-9573","authenticated-orcid":false,"given":"Da-Hye","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2024.115576_bib1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/s12560-017-9320-6","article-title":"Persistent norovirus contamination of groundwater supplies in two waterborne outbreaks","volume":"10","author":"Kauppinen","year":"2018","journal-title":"Food. Environ. Virol."},{"key":"10.1016\/j.ab.2024.115576_bib2","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1016\/j.scitotenv.2011.01.059","article-title":"Investigation of norovirus occurrence in groundwater in metropolitan Seoul, Korea","volume":"409","author":"Lee","year":"2011","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.ab.2024.115576_bib3","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.4315\/JFP-21-136","article-title":"Surveillance to prevent the spread of norovirus outbreak from asymptomatic food handlers during the PyeongChang 2018 olympics","volume":"84","author":"Jeong","year":"2021","journal-title":"J. Food Protect."},{"key":"10.1016\/j.ab.2024.115576_bib4","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1128\/CMR.00075-14","article-title":"Norovirus","volume":"28","author":"Robilotti","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.ab.2024.115576_bib5","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2022.841875","article-title":"Recent developments in isothermal amplification methods for the detection of foodborne viruses","volume":"13","author":"Suther","year":"2022","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.ab.2024.115576_bib6","doi-asserted-by":"crossref","first-page":"12162","DOI":"10.1021\/acsomega.0c00502","article-title":"Development of a simple, fast, and cost-effective nanobased immunoassay method for detecting norovirus in food samples","volume":"5","author":"Alhadrami","year":"2020","journal-title":"ACS Omega"},{"key":"10.1016\/j.ab.2024.115576_bib7","series-title":"Microbiology of the Food Chain - Horizontal Method for Determination of Hepatitis A Virus and Norovirus Using Real-Time RT-PCR - Part 2: Method for Detection. ISO 15216-2","year":"2019"},{"key":"10.1016\/j.ab.2024.115576_bib8","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1128\/JCM.02305-06","article-title":"Etiological role of viruses in outbreaks of acute gastroenteritis in The Netherlands from 1994 through 2005","volume":"45","author":"Svraka","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ab.2024.115576_bib9","doi-asserted-by":"crossref","first-page":"7891","DOI":"10.1128\/AEM.01428-07","article-title":"Evaluation of removal of noroviruses during wastewater treatment, using real-time reverse transcription-PCR: different behaviors of genogroups I and II","volume":"73","author":"Silva","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ab.2024.115576_bib10","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.jviromet.2011.04.022","article-title":"Optimization of a real-time RT-PCR assay reveals an increase of genogroup I norovirus in the clinical setting","volume":"175","author":"Van Stelten","year":"2011","journal-title":"J. Virol Methods"},{"key":"10.1016\/j.ab.2024.115576_bib11","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0160825","article-title":"Comparative evaluation of real-time PCR methods for human noroviruses in wastewater and human stool","volume":"11","author":"Masago","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.ab.2024.115576_bib12","doi-asserted-by":"crossref","first-page":"816","DOI":"10.4014\/jmb.1612.12026","article-title":"Evaluation of various real-time reverse transcription quantitative PCR assays for norovirus detection","volume":"27","author":"Ju Eun","year":"2017","journal-title":"J. Microbiol. Biotechnol."},{"key":"10.1016\/j.ab.2024.115576_bib13","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s12250-016-3863-9","article-title":"Evaluation of real-time RT-PCR assays for detection and quantification of norovirus genogroups I and II","volume":"32","author":"Rupprom","year":"2017","journal-title":"Virol. Sin."},{"key":"10.1016\/j.ab.2024.115576_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.jcv.2020.104689","article-title":"Single-step RT-PCR assay for dual genotyping of GI and GII norovirus strains","volume":"134","author":"Chhabra","year":"2021","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.ab.2024.115576_bib15","doi-asserted-by":"crossref","first-page":"38","DOI":"10.3343\/alm.2023.43.1.38","article-title":"Development and characterization of synthetic norovirus RNA for use in molecular detection methods","volume":"43","author":"Cho","year":"2023","journal-title":"Ann Lab Med"},{"key":"10.1016\/j.ab.2024.115576_bib16","doi-asserted-by":"crossref","first-page":"4724","DOI":"10.1093\/nar\/29.22.4724","article-title":"RNAMotif, an RNA secondary structure definition and search algorithm","volume":"29","author":"Macke","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ab.2024.115576_bib17","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.tibs.2022.12.002","article-title":"RNA levers and switches controlling viral gene expression","volume":"48","author":"Allan","year":"2023","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.ab.2024.115576_bib18","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1261\/rna.079623.123","article-title":"Artifacts and biases of the reverse transcription reaction in RNA-sequencing","volume":"29","author":"Verwilt","year":"2023","journal-title":"RNA"},{"key":"10.1016\/j.ab.2024.115576_bib19","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.1373\/clinchem.2004.035469","article-title":"Comparison of reverse transcriptases in gene expression analysis","volume":"50","author":"St\u00e5hlberg","year":"2004","journal-title":"Clin. Chem."},{"key":"10.1016\/j.ab.2024.115576_bib20","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1038\/225510a0","article-title":"Derivation of the secondary structure of 5S RNA from its binding of complementary oligonucleotides","volume":"225","author":"Lewis","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.ab.2024.115576_bib21","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/S1386-6532(02)00170-1","article-title":"Influence of RNA secondary structure on HEV gene amplification using reverse-transcription and nested polymerase chain reaction","volume":"27","author":"Wei","year":"2003","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.ab.2024.115576_bib22","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1261\/rna.2500605","article-title":"RNA secondary structure prediction by centroids in a Boltzmann weighted ensemble","volume":"11","author":"Ding","year":"2005","journal-title":"RNA"},{"key":"10.1016\/j.ab.2024.115576_bib23","unstructured":"C. database, COMAR Database. RM Database."},{"key":"10.1016\/j.ab.2024.115576_bib24","doi-asserted-by":"crossref","first-page":"W70","DOI":"10.1093\/nar\/gkn188","article-title":"The Vienna RNA websuite","volume":"36","author":"Gruber","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ab.2024.115576_bib25","doi-asserted-by":"crossref","first-page":"782","DOI":"10.2144\/96205bm09","article-title":"Use of elevated reverse transcription reaction temperatures in RT-PCR","volume":"20","author":"Freeman","year":"1996","journal-title":"Biotechniques"}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269724001209?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269724001209?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,6,12]],"date-time":"2024-06-12T12:54:04Z","timestamp":1718196844000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269724001209"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9]]},"references-count":25,"alternative-id":["S0003269724001209"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2024.115576","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2024,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Validation of one-step reverse transcription digital PCR assays for Norovirus GI","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2024.115576","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"115576"}}