{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T02:11:54Z","timestamp":1778638314706,"version":"3.51.4"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Humanidades, Ciencias y Tecnolog\u00edas","doi-asserted-by":"publisher","award":["242544-2014"],"award-info":[{"award-number":["242544-2014"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008688","name":"Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008688","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2025,5]]},"DOI":"10.1016\/j.ab.2025.115769","type":"journal-article","created":{"date-parts":[[2025,1,11]],"date-time":"2025-01-11T18:13:10Z","timestamp":1736619190000},"page":"115769","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Microplate spectrophotometric method for regioselective lipase screening using structured triglycerides with punicic acid as probe"],"prefix":"10.1016","volume":"700","author":[{"given":"Enrique","family":"Ordaz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osvaldo","family":"G\u00f3mez-Secundino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiram Y.","family":"Guerrero-Elias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Angeles","family":"Camacho-Ruiz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruben","family":"Espinosa-Salgado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Escobedo-Reyes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan C.","family":"Mateos-D\u00edaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9544-2917","authenticated-orcid":false,"given":"Jorge A.","family":"Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2025.115769_bib1","doi-asserted-by":"crossref","first-page":"1608","DOI":"10.1002\/(SICI)1521-3773(19980703)37:12<1608::AID-ANIE1608>3.0.CO;2-V","article-title":"Lipases: interfacial enzymes with attractive applications","volume":"37 12","author":"Schmid","year":"1998","journal-title":"Angew. Chem."},{"key":"10.1016\/j.ab.2025.115769_bib2","article-title":"Are lipases still important biocatalysts? A study of scientific publications and patents for technological forecasting","volume":"10","author":"Daiha Kde","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.ab.2025.115769_bib3","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.molcatb.2010.01.002","article-title":"Lipases for use in industrial biocatalysis: specificity of selected structural groups of lipases","volume":"65","author":"Naik","year":"2010","journal-title":"J. Mol. Catal. B Enzym."},{"key":"10.1016\/j.ab.2025.115769_bib4","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/j.procbio.2012.01.011","article-title":"Lipase promiscuity and its biochemical applications","volume":"47","author":"Kapoor","year":"2012","journal-title":"Process Biochem."},{"key":"10.1016\/j.ab.2025.115769_bib5","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/BF02534556","article-title":"Determination of lipase specificity","volume":"18","author":"Jensen","year":"1983","journal-title":"Lipids"},{"key":"10.1016\/j.ab.2025.115769_bib6","doi-asserted-by":"crossref","first-page":"305","DOI":"10.17113\/ftb.57.03.19.6025","article-title":"Lipase-catalyzed interesterification for the synthesis of medium-long-medium (MLM) structured lipids - a review","volume":"57","author":"Utama","year":"2019","journal-title":"Food Technol. Biotechnol."},{"key":"10.1016\/j.ab.2025.115769_bib7","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1007\/s11746-001-0335-7","article-title":"Determining the regioselectivity of immobilized lipases in triacylglycerol acidolysis reactions","volume":"78","author":"Chandler","year":"2001","journal-title":"J. Am. Oil Chem. Soc."},{"key":"10.1016\/j.ab.2025.115769_bib8","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1002\/chir.10183","article-title":"Lipase regio- and stereoselectivities toward three enantiomeric pairs of didecanoyl-deoxyamino-O methyl glycerol: a kinetic study by the monomolecular film technique","volume":"15","author":"Douchet","year":"2003","journal-title":"Chirality"},{"key":"10.1016\/j.ab.2025.115769_bib9","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1002\/chir.530050106","article-title":"Stereoselective hydrolysis of triglycerides by animal and microbial lipases","volume":"5","author":"Rogalska","year":"1993","journal-title":"Chirality"},{"key":"10.1016\/j.ab.2025.115769_bib10","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.biotechadv.2005.09.003","article-title":"Understanding Candida rugosa lipases: an overview","volume":"24","author":"Dom\u00ednguez de Mar\u00eda","year":"2006","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.ab.2025.115769_bib11","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1007\/s11745-004-1258-7","article-title":"Protein engineering and applications of Candida rugosa lipase isoforms","volume":"39","author":"Akoh","year":"2004","journal-title":"Lipids"},{"key":"10.1016\/j.ab.2025.115769_bib12","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1016\/j.jmb.2003.08.005","article-title":"Structural insights into the lipase\/esterase behavior in the Candida rugosa lipases family: crystal structure of the lipase 2 isoenzyme at 1.97\u00c5 resolution","volume":"332","author":"Manche\u00f1o","year":"2003","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.ab.2025.115769_bib13","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/S0927-7765(01)00309-5","article-title":"Structural basis for the kinetics of Candida rugosa Lip1 and Lip3 isoenzymes","volume":"26","author":"Pernas","year":"2002","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"10.1016\/j.ab.2025.115769_bib14","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1093\/protein\/15.7.595","article-title":"Blocking the tunnel: engineering of Candida rugosa lipase mutants with short chain length specificity","volume":"15","author":"Schmitt","year":"2002","journal-title":"Protein Eng."},{"key":"10.1016\/j.ab.2025.115769_bib15","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1080\/87559129809541148","article-title":"Structured lipids: synthesis and applications","volume":"14","author":"Lee","year":"1998","journal-title":"Food Rev. Int."},{"key":"10.1016\/j.ab.2025.115769_bib16","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1111\/j.1541-4337.2002.tb00010.x","article-title":"Structured lipids-novel fats with medical, nutraceutical, and food applications","volume":"1","author":"Osborn","year":"2002","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"10.1016\/j.ab.2025.115769_bib17","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1007\/BF02540759","article-title":"Triglyceride interesterification by lipases. 1. Cocoa butter equivalents from a fraction of palm oil","volume":"67","author":"Bloomer","year":"1990","journal-title":"J. Am. Oil Chem. Soc."},{"key":"10.1016\/j.ab.2025.115769_bib18","first-page":"167","article-title":"A breakthrough in infant formula fats, Ol, Corps Gras","volume":"4","author":"Kavanagh","year":"1997","journal-title":"Lipid"},{"key":"10.1016\/j.ab.2025.115769_bib19","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.enzmictec.2005.10.016","article-title":"Industrial applications of microbial lipases","volume":"39","author":"Hasan","year":"2006","journal-title":"Enzym. Microb. Technol."},{"key":"10.1016\/j.ab.2025.115769_bib20","doi-asserted-by":"crossref","first-page":"20271","DOI":"10.1016\/S0021-9258(17)30500-8","article-title":"Stereoselectivity of lipases. II. Stereoselective hydrolysis of triglycerides by gastric and pancreatic lipases","volume":"265","author":"Rogalska","year":"1990","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ab.2025.115769_bib21","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.jcis.2010.03.058","article-title":"Staphylococcal lipases stereoselectively hydrolyse the sn-2 position of monomolecular films of diglyceride analogs. Application to sn-2 hydrolysis of triolein","volume":"347","author":"Horchani","year":"2010","journal-title":"J. Colloid Interface Sci."},{"key":"10.1016\/j.ab.2025.115769_bib22","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1002\/(SICI)1438-9312(200002)102:2<133::AID-EJLT133>3.0.CO;2-X","article-title":"Methods for lipase detection and assay: a critical review","volume":"102","author":"Beisson","year":"2000","journal-title":"Eur. J. Lipid Sci. Technol."},{"key":"10.1016\/j.ab.2025.115769_bib23","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0927-7765(98)00099-X","article-title":"Interfacial and\/or molecular recognition by lipases of mixed monomolecular films of 1,2-dicaprin and chiral organophosphorus glyceride analogues?","volume":"13","author":"Marguet","year":"1999","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"10.1016\/j.ab.2025.115769_bib24","doi-asserted-by":"crossref","first-page":"5230","DOI":"10.1039\/c3an36699e","article-title":"Development of a high-throughput assay for measuring lipase activity using natural triacylglycerols coated on microtiter plates","volume":"138","author":"Serveau-Avesque","year":"2013","journal-title":"Analyst"},{"key":"10.1016\/j.ab.2025.115769_bib25","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1194\/jlr.D019489","article-title":"An ultraviolet spectrophotometric assay for the screening of sn-2-specific lipases using 1,3-O-dioleoyl-2-O-\u03b1-eleostearoyl-sn-glycerol as substrate","volume":"53","author":"Mendoza","year":"2012","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.ab.2025.115769_bib26","series-title":"Method for the High-Speed Screening of Lipase Activity And\/or Lipase Inhibitors in Biological Samples and in Culture Media","first-page":"1","author":"Cavalier","year":"2013"},{"key":"10.1016\/j.ab.2025.115769_bib27","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1006\/abio.2001.5427","article-title":"An ultraviolet spectrophotometric assay for measuring lipase activity using long-chain triacyglycerols from Aleurites fordii seeds","volume":"303","author":"Pencreac\u2019h","year":"2002","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2025.115769_bib28","doi-asserted-by":"crossref","first-page":"5863","DOI":"10.3390\/molecules27185863","article-title":"Cold-pressed pomegranate seed oil: study of punicic acid properties by coupling of GC\/FID and FTIR","volume":"27","author":"Zieli\u0144ska","year":"2022","journal-title":"Molecules"},{"key":"10.1016\/j.ab.2025.115769_bib29","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.biochi.2015.09.004","article-title":"New lipase assay using Pomegranate oil coating in microtiter plates","volume":"120","author":"\u00dclker","year":"2016","journal-title":"Biochimie"},{"key":"10.1016\/j.ab.2025.115769_bib30","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s10529-018-2630-4","article-title":"Comparative features between recombinant lipases CALA-like from U. maydis and CALA from C. antarctica in thermal stability and selectivity","volume":"41","author":"Robles-Machuca","year":"2019","journal-title":"Biotechnol. Lett."},{"key":"10.1016\/j.ab.2025.115769_bib31","doi-asserted-by":"crossref","first-page":"2205","DOI":"10.1021\/bi0616089","article-title":"Probing the opening of the pancreatic lipase lid using site-directed spin labeling and EPR spectroscopy","volume":"46","author":"Belle","year":"2007","journal-title":"Biochemistry"},{"key":"10.1016\/j.ab.2025.115769_bib32","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1006\/abbi.2000.2235","article-title":"Recombinant expression and characterization of the Candida rugosa lip4 lipase in Pichia pastoris: comparison of glycosylation, activity, and stability","volume":"387","author":"Tang","year":"2001","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.ab.2025.115769_bib33","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1007\/s00253-023-12856-y","article-title":"Long chain capsaicin analogues synthetized by CALB-CLEAs show cytotoxicity on glioblastoma cell lines","volume":"108","author":"Diaz-Vidal","year":"2024","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.ab.2025.115769_bib34","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1002\/anie.197805221","article-title":"Simple method for the esterification of carboxylic acids","volume":"17","author":"Neises","year":"1978","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.ab.2025.115769_bib35","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2025.115769_bib36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-4939-0366-5_1","article-title":"Protein structure modeling with MODELLER","volume":"1137","author":"Webb","year":"2014","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.ab.2025.115769_bib37","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/1758-2946-4-17","article-title":"Avogadro: an advanced semantic chemical editor, visualization, and analysis platform","volume":"4","author":"Hanwell","year":"2012","journal-title":"J. Cheminf."},{"key":"10.1016\/j.ab.2025.115769_bib38","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"Trott","year":"2010","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.ab.2025.115769_bib39","series-title":"The PyMOL Molecular Graphics System","author":"Schrodinger","year":"2015"},{"key":"10.1016\/j.ab.2025.115769_bib40","doi-asserted-by":"crossref","first-page":"W374","DOI":"10.1093\/nar\/gky380","article-title":"CavityPlus: a web server for protein cavity detection with pharmacophore modelling, allosteric site identification and covalent ligand binding ability prediction","volume":"46","author":"Xu","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ab.2025.115769_bib41","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s00253-012-3903-9","article-title":"The short form of the recombinant CAL-A-type lipase UM03410 from the smut fungus Ustilago maydis exhibits an inherent trans-fatty acid selectivity","volume":"94","author":"Brundiek","year":"2012","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.ab.2025.115769_bib42","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.ab.2007.11.036","article-title":"Novel chromatographic resolution of chiral diacylglycerols and analysis of the stereoselective hydrolysis of triacylglycerols by lipases","volume":"375","author":"Rodriguez","year":"2008","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2025.115769_bib43","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1002\/pro.5560030111","article-title":"Two conformational states of Candida rugosa lipase","volume":"3","author":"Grochulski","year":"1994","journal-title":"Protein Sci."},{"key":"10.1016\/j.ab.2025.115769_bib44","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1002\/(SICI)1097-0061(19980915)14:12<1069::AID-YEA303>3.0.CO;2-K","article-title":"Candida rugosa lipases: molecular biology and versatility in biotechnology","volume":"14","author":"Benjamin","year":"1998","journal-title":"Yeast"},{"key":"10.1016\/j.ab.2025.115769_bib45","doi-asserted-by":"crossref","first-page":"2489","DOI":"10.1021\/acs.jmedchem.0c02227","article-title":"Decision making in structure-based drug discovery: visual inspection of docking results","volume":"64","author":"Fischer","year":"2021","journal-title":"J. Med. Chem."}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269725000065?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269725000065?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T01:41:55Z","timestamp":1778636515000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269725000065"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":45,"alternative-id":["S0003269725000065"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2025.115769","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Microplate spectrophotometric method for regioselective lipase screening using structured triglycerides with punicic acid as probe","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2025.115769","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"115769"}}