{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T08:32:35Z","timestamp":1792139555102,"version":"4.4.0"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.ab.2025.115902","type":"journal-article","created":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T06:19:25Z","timestamp":1746771565000},"page":"115902","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Univariate versus multivariate approaches for resolving the overlapped spectra of azelastine hydrochloride and mometasone furoate"],"prefix":"10.1016","volume":"704","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2519-9699","authenticated-orcid":false,"given":"Alaa","family":"Reda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hawa","family":"Khalil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman A.","family":"Bahgat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael Gamal","family":"Fawzy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2025.115902_bib1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.microc.2021.106982","article-title":"HPLC-DAD approach for determination of pyridoxine HCl and doxylamine succinate in pure and pharmaceutical dosage forms: a green stability-indicating assay method","volume":"172","author":"Abd El-Hadi","year":"2022","journal-title":"Microchem. J."},{"key":"10.1016\/j.ab.2025.115902_bib2","doi-asserted-by":"crossref","first-page":"159","DOI":"10.4155\/bio-2019-0284","article-title":"Statistical optimization and validation of a novel ultra-performance liquid chromatography method for estimation of nintedanib in rat and human plasma","volume":"12","author":"Shukla","year":"2020","journal-title":"Bioanalysis"},{"key":"10.1016\/j.ab.2025.115902_bib3","series-title":"Data Handling in Science and Technology","article-title":"Handbook of chemometrics and qualimetrics","author":"Vandeginste","year":"1997"},{"key":"10.1016\/j.ab.2025.115902_bib4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.saa.2017.08.066","article-title":"Different spectrophotometric methods applied for the analysis of simeprevir in the presence of its oxidative degradation product: acomparative study","volume":"190","author":"Attia","year":"2018","journal-title":"Spectrochim. Acta Part A Mol. Biomol. Spectrosc."},{"key":"10.1016\/j.ab.2025.115902_bib5","doi-asserted-by":"crossref","first-page":"40","DOI":"10.22159\/ijpps.2016v8i10.13537","article-title":"Recent development in ultraviolet spectrophotometry through the last decade (2006-2016): a review","volume":"8","author":"Lotfy","year":"2016","journal-title":"Int. J. Pharm. Pharm. Sci."},{"key":"10.1016\/j.ab.2025.115902_bib6","series-title":"Chemometrics in Spectroscopy","author":"Mark","year":"2010"},{"key":"10.1016\/j.ab.2025.115902_bib7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.saa.2021.120308","article-title":"Chemometric quality assessment of paracetamol and phenylephrine hydrochloride with paracetamol impurities; comparative UV-spectrophotometric implementation of four predictive models","volume":"265","author":"Farid","year":"2022","journal-title":"Spectrochim. Acta Part A Mol. Biomol. Spectrosc."},{"key":"10.1016\/j.ab.2025.115902_bib8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-025-89187-7","article-title":"UV-Vis spectroscopy coupled with firefly algorithm-enhanced artificial neural networks for the determination of propranolol, rosuvastatin, and valsartan in ternary mixtures","volume":"15","author":"Serag","year":"2025","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ab.2025.115902_bib9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.saa.2021.120334","article-title":"Analysis of the ternary antiretroviral therapy dolutegravir, lamivudine and abacavir using UV spectrophotometry and chemometric tools","volume":"264","author":"Serag","year":"2022","journal-title":"Spectrochim. Acta Part A Mol. Biomol. Spectrosc."},{"key":"10.1016\/j.ab.2025.115902_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.saa.2020.119042","article-title":"An evaluation of different bio-inspired feature selection techniques on multivariate calibration models in spectroscopy","volume":"246","author":"El-Zeiny","year":"2021","journal-title":"Spectrochim. Acta Part A Mol. Biomol. Spectrosc."},{"key":"10.1016\/j.ab.2025.115902_bib11","series-title":"Martindale: the Complete Drug Reference","author":"Sweetman","year":"2009"},{"key":"10.1016\/j.ab.2025.115902_bib12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41572-020-00227-0","article-title":"Allergic rhinitis","volume":"6","author":"Bousquet","year":"2020","journal-title":"Nat. Rev. Dis. Primers"},{"key":"10.1016\/j.ab.2025.115902_bib13","doi-asserted-by":"crossref","first-page":"3383","DOI":"10.1111\/all.15044","article-title":"Advances and highlights in allergic rhinitis","volume":"76","author":"Zhang","year":"2021","journal-title":"Allergy"},{"key":"10.1016\/j.ab.2025.115902_bib14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmed.2022.874114","article-title":"Allergic rhinitis: a clinical and pathophysiological overview","volume":"9","author":"Nur Husna","year":"2022","journal-title":"Front. Med."},{"key":"10.1016\/j.ab.2025.115902_bib15","doi-asserted-by":"crossref","first-page":"44","DOI":"10.17116\/otorino201782544-47","article-title":"The effectiveness of the combination of azelastine hydrochloride and mometasone furoate for the intranasal application in the patients presenting with seasonal allergic rhinitis","volume":"82","author":"Sa","year":"2017","journal-title":"Vestn. Otorinolaringol."},{"key":"10.1016\/j.ab.2025.115902_bib16","series-title":"United States Pharmacopeia and National Formulary (USP 37-NF 32),United States Pharmacopeial Convention","year":"2020"},{"key":"10.1016\/j.ab.2025.115902_bib17","series-title":"The British Pharmacopoeia","volume":"7 th","year":"2020"},{"key":"10.1016\/j.ab.2025.115902_bib18","first-page":"1","article-title":"Assay method development and validation for simultaneous estimation of mometasone furoate and azelastine HCL for nasal spray by RP-HPLC","volume":"1","author":"Dighe","year":"2019","journal-title":"J. Pharm. Qual. Assur. Qual. Control"},{"key":"10.1016\/j.ab.2025.115902_bib19","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1093\/jaoacint\/qsae016","article-title":"Different eco-friendly spectrophotometric approaches including direct and manipulations of zero and ratio spectra for simultaneous determination of novel nasal spray combination used in seasonal allergic rhinitis","volume":"107","author":"Reda","year":"2024","journal-title":"J. AOAC Int."},{"key":"10.1016\/j.ab.2025.115902_bib20","doi-asserted-by":"crossref","first-page":"248","DOI":"10.22159\/ajpcr.2018.v11i8.25506","article-title":"Stability indicating high performance liquid chromatography method for determination of antihistamine drug azelastine","volume":"11","author":"Patel","year":"2018","journal-title":"Asian J. Pharm. Clin. Res."},{"key":"10.1016\/j.ab.2025.115902_bib21","first-page":"589","article-title":"Determination of mometasone furoate by HPLC in topical preparations: validation","volume":"8","author":"Vairale","year":"2009","journal-title":"Trade Sci Inc"},{"key":"10.1016\/j.ab.2025.115902_bib22","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1039\/a703654j","article-title":"Multilevel multifactor designs for multivariate calibration","volume":"122","author":"Brereton","year":"1997","journal-title":"Analyst"},{"key":"10.1016\/j.ab.2025.115902_bib23","article-title":"Glenmark launches Ryaltris\u00ae-AZ Nasal Spray for the treatment of moderate to severe allergic rhinitis","year":"2021","journal-title":"India"},{"key":"10.1016\/j.ab.2025.115902_bib24","first-page":"317","article-title":"Bivariate versus multivariate smart spectrophotometric calibration methods for the simultaneous determination of a quaternary mixture of mosapride, pantoprazole and their degradation products","volume":"68","author":"Hegazy","year":"2013","journal-title":"Die Pharm. Int. J. Pharm. Sci."},{"key":"10.1016\/j.ab.2025.115902_bib25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.saa.2020.119013","article-title":"In vitro analytical dissolution profiling of antiemetic delayed release tablets in two different dissolution media: validated spectrophotometric methods versus reported HPLC","volume":"246","author":"Eltanany","year":"2021","journal-title":"Spectrochim. Acta Part A Mol. Biomol. Spectrosc."},{"key":"10.1016\/j.ab.2025.115902_bib26","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.1007\/s13738-019-01699-y","article-title":"Advanced spectrophotometric methods for quantitative assessment of two antihypertensive drugs in mixture with hydrochlorothiazide in pure form and pharmaceutical formulations","volume":"16","author":"Salem","year":"2019","journal-title":"J. Iran. Chem. Soc."},{"key":"10.1016\/j.ab.2025.115902_bib27","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.saa.2013.04.064","article-title":"Simultaneous determination of some cholesterol-lowering drugs in their binary mixture by novel spectrophotometric methods","volume":"113","author":"Lotfy","year":"2013","journal-title":"Spectrochim. Acta Part A Mol. Biomol. Spectrosc."},{"key":"10.1016\/j.ab.2025.115902_bib28","first-page":"5","volume":"vol. 1","author":"Guideline","year":"2005"},{"key":"10.1016\/j.ab.2025.115902_bib29","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1186\/s13065-023-00956-9","article-title":"Univariate versus multivariate spectrophotometric data analysis of triamterene and xipamide; a quantitative and qualitative greenly profiled comparative study","volume":"17","author":"Abd El-Hadi","year":"2023","journal-title":"BMC Chem"},{"key":"10.1016\/j.ab.2025.115902_bib30","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1021\/ac00162a020","article-title":"Partial least-squares methods for spectral analyses. 1. Relation to other quantitative calibration methods and the extraction of qualitative information","volume":"60","author":"Haaland","year":"1988","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ab.2025.115902_bib31","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1080\/22297928.2016.1153980","article-title":"Spectrophotometric and chemometric study for the simultaneous determination of mometasone furoate and miconazole nitrate in the presence of pharmaceutical dosage form additive","volume":"6","author":"Merey","year":"2016","journal-title":"Anal. Chem. Lett"},{"key":"10.1016\/j.ab.2025.115902_bib32","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1039\/b200243b","article-title":"Multivariate curve resolution of overlapping voltammetric peaks: quantitative analysis of binary and quaternary metal mixtures","volume":"127","author":"Antunes","year":"2002","journal-title":"Analyst"},{"key":"10.1016\/j.ab.2025.115902_bib33","doi-asserted-by":"crossref","DOI":"10.1155\/2020\/1684172","article-title":"Simultaneous determination of drugs affecting central nervous system (CNS) in bulk and pharmaceutical formulations using multivariate curve resolution\u2010alternating least squares (MCR\u2010ALS)","volume":"2020","author":"Shaaban","year":"2020","journal-title":"J. Anal. Methods Chem."},{"key":"10.1016\/j.ab.2025.115902_bib34","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1021\/ac00014a016","article-title":"Interactive self-modeling mixture analysis","volume":"63","author":"Windig","year":"1991","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ab.2025.115902_bib35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-024-51885-z","article-title":"Simultaneous determination of meloxicam and bupivacaine via a novel modified dual wavelength method and an advanced chemometric approach","volume":"14","author":"El-Malla","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.ab.2025.115902_bib36","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1080\/00387010.2019.1639760","article-title":"Artificial neural networks versus partial least squares and multivariate resolution-alternating least squares approaches for the assay of ascorbic acid, rutin, and hesperidin in an antioxidant formulation","volume":"52","author":"Tantawy","year":"2019","journal-title":"Spectrosc. Lett."},{"key":"10.1016\/j.ab.2025.115902_bib37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.saa.2021.119505","article-title":"Simultaneous spectrophotometric quantitative analysis of elbasvir and grazoprevir using assisted chemometric models","volume":"252","author":"Zeid","year":"2021","journal-title":"Spectrochim. Acta Part A Mol. Biomol. Spectrosc."},{"key":"10.1016\/j.ab.2025.115902_bib38","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1186\/s13065-023-01095-x","article-title":"Novel analytical method based on chemometric models applied to UV\u2013Vis spectrophotometric data for simultaneous determination of Etoricoxib and Paracetamol in presence of Paracetamol impurities","volume":"17","author":"Rahman","year":"2023","journal-title":"BMC Chem"},{"key":"10.1016\/j.ab.2025.115902_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.microc.2024.110276","article-title":"Sustainable chemometric methods boosted by Latin hypercube technique for quantifying the recently FDA-approved combination of bupivacaine and meloxicam in the presence of bupivacaine carcinogenic impurity: comprehensive greenness, blueness, and whiteness","volume":"200","author":"Halim","year":"2024","journal-title":"Microchem. J."},{"key":"10.1016\/j.ab.2025.115902_bib40","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1186\/s13065-024-01158-7","article-title":"Four chemometric models enhanced by Latin hypercube sampling design for quantification of anti-COVID drugs: sustainability profiling through multiple greenness, carbon footprint, blueness, and whiteness metrics","volume":"18","author":"Katamesh","year":"2024","journal-title":"BMC Chem"},{"key":"10.1016\/j.ab.2025.115902_bib41","doi-asserted-by":"crossref","first-page":"7598","DOI":"10.1039\/D3GC02347H","article-title":"Blue applicability grade index (BAGI) and software: a new tool for the evaluation of method practicality","volume":"25","author":"Manousi","year":"2023","journal-title":"Green Chem."},{"key":"10.1016\/j.ab.2025.115902_bib42","first-page":"1","article-title":"Assessing the greenness and environmental friendliness of analytical methods: modern approaches and recent computational programs","author":"Semysim","year":"2024","journal-title":"Crit. Rev. Anal. Chem."},{"key":"10.1016\/j.ab.2025.115902_bib43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.trac.2021.116223","article-title":"White analytical chemistry: an approach to reconcile the principles of green analytical chemistry and functionality","volume":"138","author":"Nowak","year":"2021","journal-title":"TrAC Trends Anal. Chem."},{"key":"10.1016\/j.ab.2025.115902_bib44","series-title":"Chemometrics: A Practical Guide","author":"Beebe","year":"1998"}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S000326972500140X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S000326972500140X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T03:58:51Z","timestamp":1749009531000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S000326972500140X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":44,"alternative-id":["S000326972500140X"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2025.115902","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Univariate versus multivariate approaches for resolving the overlapped spectra of azelastine hydrochloride and mometasone furoate","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2025.115902","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"115902"}}