{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T05:32:42Z","timestamp":1793251962280,"version":"4.5.0"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T00:00:00Z","timestamp":1763510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009326","name":"Cancer Research Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009326","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["PJT- 183859"],"award-info":[{"award-number":["PJT- 183859"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.ab.2025.116015","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T14:42:20Z","timestamp":1763649740000},"page":"116015","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Rapid purification of DNA samples from dextran sodium sulfate (DSS) contaminants"],"prefix":"10.1016","volume":"709","author":[{"given":"Adam","family":"Yeh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Saliba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7029-2462","authenticated-orcid":false,"given":"Volker","family":"Blank","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2025.116015_bib1","first-page":"1327","article-title":"[Production of experimental ulcerative colitis in hamsters by dextran sulfate sodium and changes in intestinal microflora]","volume":"82","author":"Ohkusa","year":"1985","journal-title":"Nihon Shokakibyo Gakkai Zasshi"},{"key":"10.1016\/j.ab.2025.116015_bib2","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1016\/0016-5085(90)90290-H","article-title":"A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice","volume":"98","author":"Okayasu","year":"1990","journal-title":"Gastroenterology"},{"issue":"15","key":"10.1016\/j.ab.2025.116015_bib3","first-page":"25 11","article-title":"Dextran sulfate sodium (DSS)-induced colitis in mice","volume":"104","author":"Chassaing","year":"2014","journal-title":"Curr. Protoc. Im."},{"key":"10.1016\/j.ab.2025.116015_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2023.102105","article-title":"Protocol for colitis-associated colorectal cancer murine model induced by AOM and DSS","volume":"4","author":"Sun","year":"2023","journal-title":"STAR Protoc."},{"key":"10.1016\/j.ab.2025.116015_bib5","doi-asserted-by":"crossref","DOI":"10.1155\/2012\/718617","article-title":"Dextran sodium sulphate colitis mouse model: traps and tricks","volume":"2012","author":"Perse","year":"2012","journal-title":"J. Biomed. Biotechnol."},{"key":"10.1016\/j.ab.2025.116015_bib6","first-page":"45","article-title":"An experimental model of colitis induced by dextran sulfate sodium from acute progresses to chronicity in C57BL\/6: correlation between conditions of mice and the environment","volume":"9","author":"Taghipour","year":"2016","journal-title":"Gastro. Hepatol Bed Bench"},{"key":"10.1016\/j.ab.2025.116015_bib7","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1023\/B:DDAS.0000026298.72088.f7","article-title":"Impact of dextran sulfate sodium load on the severity of inflammation in experimental colitis","volume":"49","author":"Vowinkel","year":"2004","journal-title":"Dig. Dis. Sci."},{"key":"10.1016\/j.ab.2025.116015_bib8","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.jss.2006.07.050","article-title":"Loss of the tight junction protein ZO-1 in dextran sulfate sodium induced colitis","volume":"140","author":"Poritz","year":"2007","journal-title":"J. Surg. Res."},{"key":"10.1016\/j.ab.2025.116015_bib9","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1093\/ibd\/izad312","article-title":"Unveiling colitis: a journey through the dextran sodium sulfate-induced model","volume":"30","author":"Yang","year":"2024","journal-title":"Inflamm. Bowel Dis."},{"key":"10.1016\/j.ab.2025.116015_bib10","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1136\/gut.2009.197822","article-title":"Colitis induced in mice with dextran sulfate sodium (DSS) is mediated by the NLRP3 inflammasome","volume":"59","author":"Bauer","year":"2010","journal-title":"Gut"},{"key":"10.1016\/j.ab.2025.116015_bib11","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0032084","article-title":"Dextran sodium sulfate (DSS) induces colitis in mice by forming nano-lipocomplexes with medium-chain-length fatty acids in the colon","volume":"7","author":"Laroui","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.ab.2025.116015_bib12","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1186\/1756-0500-6-360","article-title":"Dextran sodium sulfate inhibits the activities of both polymerase and reverse transcriptase: lithium chloride purification, a rapid and efficient technique to purify RNA","volume":"6","author":"Viennois","year":"2013","journal-title":"BMC Res. Notes"},{"key":"10.1016\/j.ab.2025.116015_bib13","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1002\/ibd.21763","article-title":"Dextran sodium sulfate inhibition of real-time polymerase chain reaction amplification: a poly-A purification solution","volume":"18","author":"Kerr","year":"2012","journal-title":"Inflamm. Bowel Dis."},{"key":"10.1016\/j.ab.2025.116015_bib14","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.ab.2019.05.011","article-title":"Rapid removal of dextran sulfate sodium from tissue RNA preparations for measurement of inflammation biomarkers","volume":"579","author":"Juritsch","year":"2019","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2025.116015_bib15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mimet.2017.10.015","article-title":"Have you tried spermine? A rapid and cost-effective method to eliminate dextran sodium sulfate inhibition of PCR and RT-PCR","volume":"144","author":"Krych","year":"2018","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.ab.2025.116015_bib16","doi-asserted-by":"crossref","first-page":"8014","DOI":"10.1073\/pnas.81.24.8014","article-title":"Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics","volume":"81","author":"Saghai-Maroof","year":"1984","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ab.2025.116015_bib17","series-title":"Plant Molecular Manual","first-page":"73","article-title":"Extraction of DNA from plant tissues","author":"Rogers","year":"1989"},{"key":"10.1016\/j.ab.2025.116015_bib18","first-page":"13","article-title":"Isolation of plant DNA from fresh tissue","volume":"12","author":"Doyle","year":"1990","journal-title":"Focus"},{"key":"10.1016\/j.ab.2025.116015_bib19","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1186\/2193-1801-2-669","article-title":"An efficient protocol for total DNA extraction from the members of order Zingiberales- suitable for diverse PCR based downstream applications","volume":"2","author":"Devi","year":"2013","journal-title":"SpringerPlus"},{"key":"10.1016\/j.ab.2025.116015_bib20","article-title":"Preparation of genomic DNA from bacteria","volume":"2","author":"Wilson","year":"2001","journal-title":"Curr. Protoc. Mol. Biol."},{"key":"10.1016\/j.ab.2025.116015_bib21","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1007\/s10528-006-9050-1","article-title":"A widely applicable protocol for DNA isolation from fecal samples","volume":"44","author":"Zhang","year":"2006","journal-title":"Biochem. Genet."},{"key":"10.1016\/j.ab.2025.116015_bib22","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2020.535310","article-title":"Fecal and serum metabolomic signatures and microbial community profiling of postmenopausal osteoporosis mice model","volume":"10","author":"Wen","year":"2020","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.ab.2025.116015_bib23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2024\/6653560","article-title":"Variations in gut microbiome profiles based on different DNA extraction methods: a comparative study","author":"Liu","year":"2024","journal-title":"Adv. Gut Microbiome Res."},{"key":"10.1016\/j.ab.2025.116015_bib24","doi-asserted-by":"crossref","DOI":"10.21769\/BioProtoc.2707","article-title":"Extraction of DNA from murine fecal pellets for downstream phylogenetic microbiota analysis by next-generation sequencing","volume":"8","author":"Eeckhout","year":"2018","journal-title":"Bio Protoc."},{"key":"10.1016\/j.ab.2025.116015_bib25","series-title":"Pihms_Sop 07 V1: Standard Operating Procedure for Fecal Samples DNA Extraction","author":"Standards","year":"2016"},{"key":"10.1016\/j.ab.2025.116015_bib26","article-title":"Inhibition of polymerase chain reaction by hydrogel monomers","volume":"45","author":"Tran","year":"2025","journal-title":"Appl. Mater. Today"},{"key":"10.1016\/j.ab.2025.116015_bib27","doi-asserted-by":"crossref","first-page":"e40","DOI":"10.1093\/nar\/gkn1055","article-title":"Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples","volume":"37","author":"Kermekchiev","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ab.2025.116015_bib28","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1128\/JCM.42.1.408-411.2004","article-title":"Impact of DNA polymerases and their buffer systems on quantitative real-time PCR","volume":"42","author":"Wolffs","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ab.2025.116015_bib29","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1128\/jcm.35.4.995-998.1997","article-title":"Complex polysaccharides as PCR inhibitors in feces: helicobacter pylori model","volume":"35","author":"Monteiro","year":"1997","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ab.2025.116015_bib30","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.syapm.2017.11.008","article-title":"Comparison of DNA extraction methods for human gut microbial community profiling","volume":"41","author":"Lim","year":"2018","journal-title":"Syst. Appl. Microbiol."}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269725002544?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269725002544?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T05:00:17Z","timestamp":1793250017000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269725002544"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":30,"alternative-id":["S0003269725002544"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2025.116015","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Rapid purification of DNA samples from dextran sodium sulfate (DSS) contaminants","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2025.116015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"116015"}}