{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T06:46:08Z","timestamp":1792392368865,"version":"4.4.0"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100009620","name":"Gansu Province Science and Technology Department","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009620","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2027,2]]},"DOI":"10.1016\/j.ab.2026.116268","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T15:42:50Z","timestamp":1791992570000},"page":"116268","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Stepwise derivatization GC-MS\/MS of 39 FFAs in spleen-deficiency rat liver"],"prefix":"10.1016","volume":"721","author":[{"given":"Lingyu","family":"Dun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxing","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longxia","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanping","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0149-2855","authenticated-orcid":false,"given":"Fangdi","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"8074","key":"10.1016\/j.ab.2026.116268_bib1","doi-asserted-by":"crossref","first-page":"1428","DOI":"10.1038\/s41586-025-09186-6","article-title":"Allosteric modulation and biased signalling at free fatty acid receptor 2","volume":"643","author":"Zhang","year":"2025","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.ab.2026.116268_bib2","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1152\/physrev.00041.2018","article-title":"Free fatty acid receptors in health and disease","volume":"100","author":"Kimura","year":"2020","journal-title":"Physiol. Rev."},{"issue":"5","key":"10.1016\/j.ab.2026.116268_bib3","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/j.cmet.2023.03.018","article-title":"FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity","volume":"35","author":"Wieder","year":"2023","journal-title":"Cell Metab."},{"issue":"11","key":"10.1016\/j.ab.2026.116268_bib4","doi-asserted-by":"crossref","first-page":"2437","DOI":"10.1016\/j.cmet.2024.10.001","article-title":"Amino acid is a major carbon source for hepatic lipogenesis","volume":"36","author":"Liao","year":"2024","journal-title":"Cell Metab."},{"issue":"27","key":"10.1016\/j.ab.2026.116268_bib5","doi-asserted-by":"crossref","first-page":"3290","DOI":"10.3748\/wjg.v30.i27.3290","article-title":"Targeted metabolomics study of fatty-acid metabolism in lean metabolic-associated fatty liver disease patients","volume":"30","author":"Sun","year":"2024","journal-title":"World J. Gastroenterol."},{"issue":"7","key":"10.1016\/j.ab.2026.116268_bib6","doi-asserted-by":"crossref","DOI":"10.1002\/ddr.70014","article-title":"The role of fatty acid metabolism, the related potential biomarkers, and targeted therapeutic strategies in gastrointestinal cancers","volume":"85","author":"Xie","year":"2024","journal-title":"Drug Dev. Res."},{"issue":"9","key":"10.1016\/j.ab.2026.116268_bib7","doi-asserted-by":"crossref","first-page":"3949","DOI":"10.1016\/j.apsb.2024.05.014","article-title":"Schisanhenol ameliorates non-alcoholic fatty liver disease via inhibiting miR-802 activation of AMPK-Mediated modulation of hepatic lipid metabolism","volume":"14","author":"Li","year":"2024","journal-title":"Acta Pharm. Sin. B"},{"issue":"3","key":"10.1016\/j.ab.2026.116268_bib8","doi-asserted-by":"crossref","first-page":"73","DOI":"10.3892\/ijmm.2024.5397","article-title":"Salusin-\u03b1 alleviates lipid metabolism disorders via regulation of the downstream lipogenesis genes through the LKB1\/AMPK pathway","volume":"54","author":"Pan","year":"2024","journal-title":"Int. J. Mol. Med."},{"issue":"7","key":"10.1016\/j.ab.2026.116268_bib9","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.3390\/nu17071203","article-title":"Mechanisms linking obesity, insulin resistance, and Alzheimer'S disease: effects of polyphenols and omega-3 polyunsaturated fatty acids","volume":"17","author":"Yavari","year":"2025","journal-title":"Nutrients"},{"issue":"15","key":"10.1016\/j.ab.2026.116268_bib10","doi-asserted-by":"crossref","first-page":"7900","DOI":"10.1021\/acs.analchem.7b00848","article-title":"Orthogonal comparison of GC-MS and 1H NMR Spectsoscopy for ShortsChaincFattyfAcid auantqtation","volume":"89","author":"Cai","year":"2017","journal-title":"Anal. Chem."},{"issue":"5","key":"10.1016\/j.ab.2026.116268_bib11","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1021\/acs.jproteome.8b00536","article-title":"Evaluation and optimization of sample handling methods for quantification of short-chain fatty acids in human fecal samples by GC-MS","volume":"18","author":"Hsu","year":"2019","journal-title":"J. Proteome Res."},{"issue":"14","key":"10.1016\/j.ab.2026.116268_bib12","doi-asserted-by":"crossref","first-page":"5150","DOI":"10.1021\/ac070311t","article-title":"Enhancement of the LC\/MS analysis of fatty acids through derivatization and stable isotope coding","volume":"79","author":"Yang","year":"2007","journal-title":"Anal. Chem."},{"issue":"6","key":"10.1016\/j.ab.2026.116268_bib13","doi-asserted-by":"crossref","first-page":"299","DOI":"10.52601\/bpr.2023.230042","article-title":"Rapid quantification of 50 fatty acids in small amounts of biological samples for population molecular phenotyping","volume":"9","author":"Liu","year":"2023","journal-title":"Biophys Rep"},{"issue":"2","key":"10.1016\/j.ab.2026.116268_bib14","doi-asserted-by":"crossref","first-page":"104","DOI":"10.3390\/metabo15020104","article-title":"A new, validated GC-PICI-MS method for the quantification of 32 lipid fatty acids via base-catalyzed transmethylation and the isotope-coded derivatization of internal standards","volume":"15","author":"Vodr\u00e1\u017eka","year":"2025","journal-title":"Metabolites"},{"issue":"9","key":"10.1016\/j.ab.2026.116268_bib15","doi-asserted-by":"crossref","first-page":"2371","DOI":"10.1007\/s00216-024-05185-0","article-title":"Sample preparation for fatty acid analysis in biological samples with mass spectrometry-based strategies","volume":"416","author":"Yang","year":"2024","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.ab.2026.116268_bib16","doi-asserted-by":"crossref","first-page":"120","DOI":"10.3389\/fmolb.2019.00120","article-title":"Targeted metabolomic analysis of serum fatty acids for the prediction of autoimmune diseases","volume":"6","author":"Tsoukalas","year":"2019","journal-title":"Front. Mol. Biosci."},{"key":"10.1016\/j.ab.2026.116268_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2022.114620","article-title":"Targeted metabolomics profiles serum fatty acids by HFD induced non-alcoholic fatty liver in mice based on GC-MS","volume":"211","author":"Yan","year":"2022","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.ab.2026.116268_bib18","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.talanta.2018.12.049","article-title":"A sensitive GC\/MS detection method for analyzing microbial metabolites short chain fatty acids in fecal and serum samples","volume":"196","author":"Zhang","year":"2019","journal-title":"Talanta"},{"key":"10.1016\/j.ab.2026.116268_bib19","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.jchromb.2018.06.003","article-title":"Simultaneous quantification of 18 saturated and unsaturated fatty acids and 7 sterols as their tert-butyldimethylsilyl derivatives in human saliva using gas chromatography-tandem mass spectrometry","volume":"1092","author":"Moon","year":"2018","journal-title":"J Chromatogr B Analyt Technol Biomed Life Sci"},{"key":"10.1016\/j.ab.2026.116268_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2025.116956","article-title":"Metabolomics study by metabolite profiling analysis in kidney and liver of calorie-restricted aging rats using GC-MS\/MS","volume":"263","author":"Choi","year":"2025","journal-title":"J. Pharm. Biomed. Anal."},{"issue":"22","key":"10.1016\/j.ab.2026.116268_bib21","doi-asserted-by":"crossref","first-page":"6621","DOI":"10.1007\/s00216-022-04223-z","article-title":"Potential of atmospheric pressure ionization sources for the analysis of free fatty acids in clinical and biological samples by gas chromatography-mass spectrometry","volume":"414","author":"G\u00f6rs","year":"2022","journal-title":"Anal. Bioanal. Chem."},{"issue":"18","key":"10.1016\/j.ab.2026.116268_bib22","doi-asserted-by":"crossref","first-page":"8931","DOI":"10.1021\/acs.analchem.6b02834","article-title":"Photochemical tagging for quantitation of unsaturated fatty acids by mass spectrometry","volume":"88","author":"Ma","year":"2016","journal-title":"Anal. Chem."},{"issue":"19","key":"10.1016\/j.ab.2026.116268_bib23","first-page":"4241","article-title":"Mechanism of different processed products of codonopsis radix on spleen deficiency rats based on 1H-NMR metabolomics","volume":"44","author":"Hao","year":"2019","journal-title":"China J. Chin. Mater. Med."},{"issue":"8","key":"10.1016\/j.ab.2026.116268_bib24","first-page":"950","article-title":"Study on the immune-enhancing and spleen-strengthening effects of Zhenyuan Capsules on immunocompromised mice with spleen deficiency syndrome","volume":"43","author":"Yue","year":"2023","journal-title":"Jilin Journal of Chinese Medicine"},{"issue":"15","key":"10.1016\/j.ab.2026.116268_bib28","doi-asserted-by":"crossref","first-page":"4391","DOI":"10.1007\/s00216-021-03785-8","article-title":"Quantitative analysis of short-chain fatty acids in human plasma and serum by GC-MS","volume":"414","author":"Yao","year":"2022","journal-title":"Anal. Bioanal. Chem."},{"issue":"5","key":"10.1016\/j.ab.2026.116268_bib26","doi-asserted-by":"crossref","first-page":"443","DOI":"10.3724\/SP.J.1123.2022.09014","article-title":"Determination of 39 fatty acids in rat liver by gas chromatography-mass spectrometry","volume":"41","author":"Wu","year":"2023","journal-title":"Chin. J. Chromatogr."},{"key":"10.1016\/j.ab.2026.116268_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbrc.2024.150319","article-title":"Castration reshapes the liver by altering fatty acid composition and metabolism in male mice","volume":"727","author":"Yao","year":"2024","journal-title":"Biochem. Biophys. Res. Commun."},{"issue":"22","key":"10.1016\/j.ab.2026.116268_bib25","doi-asserted-by":"crossref","first-page":"12223","DOI":"10.1021\/acs.analchem.7b03020","article-title":"Twin derivatization strategy for high-coverage quantification of free Fatty acids by liquid chromatography-tandem mass spectrometry","volume":"89","author":"Jiang","year":"2017","journal-title":"Anal. Chem."},{"issue":"6","key":"10.1016\/j.ab.2026.116268_bib27","first-page":"584","article-title":"Application of metabolomics and microbiome analysis for revealing the endogenous mechanism of baizhu Xiaozhong San in postpartum rats with Spleen-qi deficiency","volume":"28","author":"Cao","year":"2025","journal-title":"J. Med. Food"},{"issue":"1","key":"10.1016\/j.ab.2026.116268_bib29","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1186\/s12916-017-0968-4","article-title":"Association between plasma phospholipid saturated fatty acids and metabolic markers of lipid, hepatic, inflammation and glycaemic pathways in eight European countries: a cross-sectional analysis in the EPIC-InterAct study","volume":"15","author":"Zheng","year":"2017","journal-title":"BMC Med."},{"issue":"6","key":"10.1016\/j.ab.2026.116268_bib30","article-title":"Lipid alterations in chronic liver disease and liver cancer","volume":"4","author":"Paul","year":"2022","journal-title":"JHEP Rep."},{"issue":"15","key":"10.1016\/j.ab.2026.116268_bib31","first-page":"4136","article-title":"Spleen deficiency and phlegm dampness syndrome model in rats treated by Citri Reticulatae Pericarpium based on metabolomics","volume":"47","author":"Wu","year":"2022","journal-title":"Zhongguo Zhongyao Zazhi"},{"issue":"23","key":"10.1016\/j.ab.2026.116268_bib32","doi-asserted-by":"crossref","DOI":"10.1002\/rcm.9640","article-title":"Effects of ginseng on short-chain fatty acids and intestinal microbiota in rats with spleen-qi deficiency based on gas chromatography-mass spectrometry and 16s rRNA technology","volume":"37","author":"Zhang","year":"2023","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"10.1016\/j.ab.2026.116268_bib33","doi-asserted-by":"crossref","DOI":"10.1155\/2018\/4530849","article-title":"UPLC-QTOF\/MS-Based lipidomic profiling of liver Qi-Stagnation and spleen-deficiency syndrome in patients with hyperlipidemia","volume":"2018","author":"Shenghua","year":"2018","journal-title":"Evid. Based Complement. Alternat. Med."},{"issue":"20","key":"10.1016\/j.ab.2026.116268_bib34","first-page":"82","article-title":"Effect of Scrophularia ningpoensi on rat model of spleen deficiency with water-dampness retention syndrome and its liver metabolomics analysis","volume":"23","author":"Zhao","year":"2017","journal-title":"Chin. J. Exp. Tradit. Med. Formulae"}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269726002241?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269726002241?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T06:23:20Z","timestamp":1792391000000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269726002241"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,2]]},"references-count":35,"alternative-id":["S0003269726002241"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2026.116268","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2027,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Stepwise derivatization GC-MS\/MS of 39 FFAs in spleen-deficiency rat liver","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2026.116268","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"116268"}}