{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T09:43:33Z","timestamp":1792230213627,"version":"4.4.0"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2027,10,17]],"date-time":"2027-10-17T00:00:00Z","timestamp":1823731200000},"content-version":"am","delay-in-days":258,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytical Biochemistry"],"published-print":{"date-parts":[[2027,2]]},"DOI":"10.1016\/j.ab.2026.116271","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:37:22Z","timestamp":1791959842000},"page":"116271","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Expanding the capabilities of capillary electrophoresis for determining protein-DNA binding affinities: physiological conditions and mPEG-silane coating for sticky proteins"],"prefix":"10.1016","volume":"721","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-2409-8266","authenticated-orcid":false,"given":"Kristen M.","family":"Tran","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3474-8204","authenticated-orcid":false,"given":"Caitlin V.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9543-4992","authenticated-orcid":false,"given":"Keith R.","family":"Weninger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0363-4693","authenticated-orcid":false,"given":"Dorothy A.","family":"Erie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ab.2026.116271_bib1","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1038\/nprot.2011.305","article-title":"Analysis of protein-ligand interactions by fluorescence polarization","volume":"6","author":"Rossi","year":"2011","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ab.2026.116271_bib2","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1007\/s00249-021-01529-1","article-title":"Microscale thermophoresis and additional effects measured in NanoTemper monolith instruments","volume":"50","author":"Lopez-Mendez","year":"2021","journal-title":"Eur. Biophys. J."},{"key":"10.1016\/j.ab.2026.116271_bib3","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1006\/abio.1998.3056","article-title":"Is nitrocellulose filter binding really a universal assay for Protein\u2013DNA interactions?","volume":"268","author":"Oehler","year":"1999","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ab.2026.116271_bib4","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1002\/pro.3827","article-title":"The strengths and limitations of using biolayer interferometry to monitor equilibrium titrations of biomolecules","volume":"29","author":"Weeramange","year":"2020","journal-title":"Protein Sci."},{"key":"10.1016\/j.ab.2026.116271_bib5","doi-asserted-by":"crossref","first-page":"1849","DOI":"10.1038\/nprot.2007.249","article-title":"Electrophoretic mobility shift assay (EMSA) for detecting protein\u2013nucleic acid interactions","volume":"2","author":"Hellman","year":"2007","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ab.2026.116271_bib6","doi-asserted-by":"crossref","first-page":"e14","DOI":"10.1093\/nar\/gkv898","article-title":"A high-throughput assay for the comprehensive profiling of DNA ligase fidelity","volume":"44","author":"Lohman","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ab.2026.116271_bib7","doi-asserted-by":"crossref","first-page":"4326","DOI":"10.1021\/ac052121t","article-title":"ESI-MS compatible permanent coating of glass surfaces using poly (ethylene glycol)-terminated alkoxysilanes for capillary zone electrophoretic protein separations","volume":"78","author":"Razunguzwa","year":"2006","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ab.2026.116271_bib8","doi-asserted-by":"crossref","first-page":"4304","DOI":"10.1093\/nar\/26.18.4304","article-title":"A capillary electrophoresis mobility shift assay for protein-DNA binding affinities free in solution","volume":"26","author":"Foulds","year":"1998","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ab.2026.116271_bib9","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S0021-9673(99)00923-1","article-title":"DNA-binding affinities of MyoD and E47 homo- and hetero-dimers by capillary electrophoresis mobility shift assay","volume":"862","author":"Foulds","year":"1999","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.ab.2026.116271_bib10","first-page":"369","article-title":"Protein-DNA binding affinities by capillary electrophoresis","volume":"163","author":"Foulds","year":"2001","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.ab.2026.116271_bib11","doi-asserted-by":"crossref","first-page":"4540","DOI":"10.1021\/ac980638h","article-title":"Aptamers as ligands in affinity probe capillary electrophoresis","volume":"70","author":"German","year":"1998","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ab.2026.116271_bib12","doi-asserted-by":"crossref","first-page":"7421","DOI":"10.1021\/acs.analchem.6b02117","article-title":"Analysis of DNA in phosphate buffered saline using kinetic capillary electrophoresis","volume":"88","author":"Kanoatov","year":"2016","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ab.2026.116271_bib13","doi-asserted-by":"crossref","first-page":"8532","DOI":"10.1021\/acs.analchem.9b01801","article-title":"Determination of the equilibrium constant and rate constant of protein-oligonucleotide complex dissociation under the conditions of ideal-filter capillary electrophoresis","volume":"91","author":"Le","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.ab.2026.116271_bib14","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1002\/elps.202300158","article-title":"Strategies for capillary electrophoresis: method development and validation for pharmaceutical and biological applications-updated and completely revised edition","volume":"44","author":"Krebs","year":"2023","journal-title":"Electrophoresis"},{"key":"10.1016\/j.ab.2026.116271_bib15","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1093\/nar\/27.4.1063","article-title":"The salt dependence of DNA recognition by NF-kappaB p50: a detailed kinetic analysis of the effects on affinityand specificity","volume":"27","author":"Hart","year":"1999","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ab.2026.116271_bib16","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/0076-6879(91)08018-D","article-title":"Analysis of equilibrium and kinetic measurements to determine thermodynamic origins of stability and specificity and mechanism of formation of site-specific complexes between proteins and helical DNA","volume":"208","author":"Record","year":"1991","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.ab.2026.116271_bib17","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1002\/elps.200390029","article-title":"Microchannel wall coatings for protein separations by capillary and chip electrophoresis","volume":"24","author":"Doherty","year":"2003","journal-title":"Electrophoresis"},{"key":"10.1016\/j.ab.2026.116271_bib18","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1007\/s10337-015-2895-x","article-title":"A comprehensive study of silanization and Co-Condensation for straightforward single-step covalent neutral capillary coating","volume":"78","author":"Mai","year":"2015","journal-title":"Chromatographia"},{"key":"10.1016\/j.ab.2026.116271_bib19","doi-asserted-by":"crossref","first-page":"6781","DOI":"10.1007\/s00216-016-9804-5","article-title":"Photosensitive diazotized poly (ethylene glycol) covalent capillary coatings for analysis of proteins by capillary electrophoresis","volume":"408","author":"Yu","year":"2016","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.ab.2026.116271_bib20","doi-asserted-by":"crossref","first-page":"39505","DOI":"10.1021\/acsami.8b15796","article-title":"Efficient one-step PEG-silane passivation of glass surfaces for single-molecule fluorescence studies","volume":"10","author":"Gidi","year":"2018","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.ab.2026.116271_bib21","doi-asserted-by":"crossref","first-page":"5040","DOI":"10.1074\/jbc.271.9.5040","article-title":"Identification and characterization of a thermostable MutS homolog from Thermus aquaticus","volume":"271","author":"Biswas","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ab.2026.116271_bib22","doi-asserted-by":"crossref","first-page":"13355","DOI":"10.1074\/jbc.272.20.13355","article-title":"Interaction of MutS protein with the major and minor grooves of a heteroduplex DNA","volume":"272","author":"Biswas","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ab.2026.116271_bib23","doi-asserted-by":"crossref","first-page":"23673","DOI":"10.1074\/jbc.274.33.23673","volume":"274","author":"Biswas","year":"1999","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.ab.2026.116271_bib24","doi-asserted-by":"crossref","first-page":"4192","DOI":"10.1016\/j.jmb.2013.08.011","article-title":"Slow conformational changes in MutS and DNA direct ordered transitions between mismatch search, recognition and signaling of DNA repair","volume":"425","author":"Sharma","year":"2013","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.ab.2026.116271_bib25","first-page":"1","article-title":"Large conformational changes in MutS during DNA scanning, mismatch recognition and repair signalling","author":"Qiu","year":"2012","journal-title":"EMBO J."},{"key":"10.1016\/j.ab.2026.116271_bib26","doi-asserted-by":"crossref","first-page":"5735","DOI":"10.1016\/j.cell.2025.07.003","article-title":"DNA mutagenesis driven by transcription factor competition with mismatch repair","volume":"188","author":"Zhu","year":"2025","journal-title":"Cell"},{"key":"10.1016\/j.ab.2026.116271_bib27","doi-asserted-by":"crossref","first-page":"13674","DOI":"10.1021\/ja028212e","article-title":"Nonequilibrium capillary electrophoresis of equilibrium mixtures \u2212 A single experiment reveals equilibrium and kinetic parameters of Protein\u2212DNA interactions","volume":"124","author":"Berezovski","year":"2002","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.ab.2026.116271_bib28","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1021\/bi015843x","article-title":"Dissecting the metal ion dependence of DNA binding by PvuII endonuclease","volume":"41","author":"Conlan","year":"2002","journal-title":"Biochemistry"},{"key":"10.1016\/j.ab.2026.116271_bib29","doi-asserted-by":"crossref","first-page":"2025","DOI":"10.1093\/nar\/gkg324","article-title":"DNA binding by yeast Mlh1 and Pms1: implications for DNA mismatch repair","volume":"31","author":"Hall","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ab.2026.116271_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2023.117080","article-title":"Buffering agents and additives for the background electrolyte solutions used for peptide and protein capillary zone electrophoresis","volume":"164","author":"Corradini","year":"2023","journal-title":"TrAC, Trends Anal. Chem."},{"key":"10.1016\/j.ab.2026.116271_bib31","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1021\/bi00382a006","article-title":"Replacement of potassium chloride by potassium glutamate dramatically enhances protein-DNA interactions in vitro","volume":"26","author":"Leirmo","year":"1987","journal-title":"Biochemistry"},{"key":"10.1016\/j.ab.2026.116271_bib32","doi-asserted-by":"crossref","first-page":"10956","DOI":"10.1073\/pnas.0704148104","article-title":"Chimeric Saccharomyces cerevisiae Msh6 protein with an Msh3 mispair-binding domain combines properties of both proteins","volume":"104","author":"Shell","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U. S. A"},{"key":"10.1016\/j.ab.2026.116271_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2019.460397","article-title":"Semi-permanent cationic coating for protein separations","volume":"1607","author":"Crihfield","year":"2019","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.ab.2026.116271_bib34","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1146\/annurev.ms.26.080196.002053","article-title":"Surface treatments of polymers for biocompatibility","volume":"26","author":"Elbert","year":"1996","journal-title":"Annu. Rev. Mater. Res."},{"key":"10.1016\/j.ab.2026.116271_bib35","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1002\/elps.200305714","article-title":"A very thin coating for capillary zone electrophoresis of proteins based on a tri (ethylene glycol)-terminated alkyltrichlorosilane","volume":"25","author":"Meagher","year":"2004","journal-title":"Electrophoresis"},{"key":"10.1016\/j.ab.2026.116271_bib36","first-page":"10782","article-title":"Coordinated protein and DNA conformational changes govern mismatch repair initiation by MutS","volume":"46","author":"LeBlanc","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ab.2026.116271_bib37","doi-asserted-by":"crossref","first-page":"25545","DOI":"10.1074\/jbc.M202282200","article-title":"Dominant Saccharomyces cerevisiae msh6 mutations cause increased mispair binding and decreased dissociation from mispairs by Msh2-Msh6 in the presence of ATP","volume":"277","author":"Hess","year":"2002","journal-title":"J. Biol. Chem."}],"container-title":["Analytical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269726002277?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003269726002277?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T09:09:39Z","timestamp":1792228179000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003269726002277"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,2]]},"references-count":37,"alternative-id":["S0003269726002277"],"URL":"https:\/\/doi.org\/10.1016\/j.ab.2026.116271","relation":{},"ISSN":["0003-2697"],"issn-type":[{"value":"0003-2697","type":"print"}],"subject":[],"published":{"date-parts":[[2027,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Expanding the capabilities of capillary electrophoresis for determining protein-DNA binding affinities: physiological conditions and mPEG-silane coating for sticky proteins","name":"articletitle","label":"Article Title"},{"value":"Analytical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ab.2026.116271","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"116271"}}