{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T12:44:34Z","timestamp":1760013874613},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Government of India","award":["BT\/502\/NE\/TBP\/2013"],"award-info":[{"award-number":["BT\/502\/NE\/TBP\/2013"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Archives of Biochemistry and Biophysics"],"published-print":{"date-parts":[[2016,4]]},"DOI":"10.1016\/j.abb.2016.02.025","type":"journal-article","created":{"date-parts":[[2016,2,28]],"date-time":"2016-02-28T19:20:00Z","timestamp":1456687200000},"page":"10-21","update-policy":"http:\/\/dx.doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Understanding the importance of conservative hypothetical protein LdBPK_070020 in Leishmania donovani and its role in subsistence of the parasite"],"prefix":"10.1016","volume":"596","author":[{"given":"Ruchika","family":"Bhardwaj","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ritesh","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjeev Kumar","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chandrabose","family":"Selvaraj","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vikash Kumar","family":"Dubey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"year":"2012","series-title":"First WHO Report on Neglected Tropical Diseases","author":"World Health Organization","key":"10.1016\/j.abb.2016.02.025_bib1"},{"key":"10.1016\/j.abb.2016.02.025_bib2","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.cimid.2004.03.004","article-title":"current situation and new perspectives","volume":"27","author":"Desjeux Leishmaniasis","year":"2004","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"10.1016\/j.abb.2016.02.025_bib3","first-page":"331","article-title":"Visceral leishmaniasis (kala-azar): challenges ahead","volume":"123","author":"Singh","year":"2006","journal-title":"Indian J. Med. Res."},{"key":"10.1016\/j.abb.2016.02.025_bib4","doi-asserted-by":"crossref","first-page":"2208","DOI":"10.1007\/s12010-009-8764-z","article-title":"Rational approaches for drug designing against leishmaniasis","volume":"160","author":"Shukla","year":"2010","journal-title":"Appl. Biochem. Biotechnol."},{"key":"10.1016\/j.abb.2016.02.025_bib5","first-page":"255","article-title":"Insect vectors of Leishmania: distribution, physiology and their control","volume":"45","author":"Sharma","year":"2008","journal-title":"J.\u00a0Vector Borne Dis."},{"key":"10.1016\/j.abb.2016.02.025_bib6","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1093\/cid\/cit102","article-title":"Increasing failure of miltefosine in the treatment of Kala-azar in Nepal and the potential role of parasite drug resistance, reinfection, or noncompliance","volume":"56","author":"Rijal","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.abb.2016.02.025_bib7","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/s11010-011-0762-0","article-title":"Evaluation of selected antitumor agents as subversive substrate and potential inhibitor of trypanothione reductase: an alternative approach for chemotherapy of Leishmaniasis","volume":"352","author":"Shukla","year":"2011","journal-title":"Mol. Cell. Biochem."},{"key":"10.1016\/j.abb.2016.02.025_bib8","doi-asserted-by":"crossref","first-page":"4807","DOI":"10.1111\/febs.12449","article-title":"Miltefosine unresponsive Leishmania donovani has better ability of resist reactive oxygen species","volume":"280","author":"Das","year":"2013","journal-title":"FEBS J."},{"key":"10.1016\/j.abb.2016.02.025_bib9","doi-asserted-by":"crossref","first-page":"2143","DOI":"10.1101\/gr.123430.111","article-title":"Whole genome sequencing of multiple Leishmania donovani clinical isolates provides insights into population structure and mechanisms of drug resistance","volume":"21","author":"Downing","year":"2011","journal-title":"Genome Res."},{"key":"10.1016\/j.abb.2016.02.025_bib10","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0035-9203(01)90350-5","article-title":"A\u00a0mini-exon multiplex polymerase chain reaction to distinguish the major groups of Trypanosoma cruzi and T.\u00a0rangeli in the Brazilian Amazon","volume":"95","author":"Fernandes","year":"2001","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.abb.2016.02.025_bib11","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.gene.2005.04.002","article-title":"Intragenomic spliced leader RNA array analysis of kinetoplastids reveals unexpected transcribed region diversity in Trypanosoma cruzi","volume":"352","author":"Thomas","year":"2005","journal-title":"Gene"},{"key":"10.1016\/j.abb.2016.02.025_bib12","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.ejpb.2013.08.014","article-title":"Molecular mechanism underlying antileishmanial effect of oxabicyclo[3.3.1]nonanones: inhibition of key redox enzymes of the pathogen","volume":"85","author":"Saudagar","year":"2013","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"10.1016\/j.abb.2016.02.025_bib13","doi-asserted-by":"crossref","first-page":"354","DOI":"10.4269\/ajtmh.13-0320","article-title":"Molecular mechanisms of in\u00a0vitro betulin induced apoptosis of Leishmania donovani","volume":"90","author":"Saudagar","year":"2014","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.abb.2016.02.025_bib14","first-page":"333","article-title":"Transfection of Leishmania and Trypanosoma brucei by electroporation","volume":"21","author":"Beverley","year":"1993","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.abb.2016.02.025_bib15","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/0166-6851(96)02580-7","article-title":"Use of the green fluorescent protein as a marker in transfected Leishmania","volume":"77","author":"Ha","year":"1996","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.abb.2016.02.025_bib16","doi-asserted-by":"crossref","first-page":"23266","DOI":"10.1074\/jbc.M111.229674","article-title":"LFR1 ferric iron reductase of Leishmania amazonensis is essential for the generation of infective parasite forms","volume":"286","author":"Flannery","year":"2011","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.abb.2016.02.025_bib17","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A\u00a0rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.abb.2016.02.025_bib18","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1006\/abio.1996.0160","article-title":"Detection of multiple antigens on western blots","volume":"236","author":"Krajewski","year":"1996","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.abb.2016.02.025_bib19","doi-asserted-by":"crossref","first-page":"34992","DOI":"10.1074\/jbc.M112.389338","article-title":"NAD(P)H cytochrome b5 oxidoreductase deficiency in Leishmania major results in impaired linoleate synthesis followed by increased oxidative stress and cell death","volume":"287","author":"Mukherjee","year":"2012","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.abb.2016.02.025_bib20","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0025273","article-title":"CYP5122A1, a novel cytochrome P450 is essential for survival of Leishmania donovani","volume":"6","author":"Verma","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.abb.2016.02.025_bib21","doi-asserted-by":"crossref","first-page":"2461","DOI":"10.1091\/mbc.02-03-0034","article-title":"Large-scale isolation of Cajal bodies from HeLa cells","volume":"13","author":"Lam","year":"2002","journal-title":"Mol. Biol. Cell."},{"key":"10.1016\/j.abb.2016.02.025_bib22","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1038\/sj.cdd.4400959","article-title":"Role of glutathione depletion and reactive oxygen species generation in apoptotic signaling in a human B lymphoma cell line","volume":"9","author":"Armstrong","year":"2002","journal-title":"Cell Death Differ."},{"key":"10.1016\/j.abb.2016.02.025_bib23","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1007\/s13277-010-0152-3","article-title":"Erucylphosphocholine induces growth inhibition, cell cycle arrest, and apoptosis in human choriocarcinoma cells","volume":"32","author":"Takai","year":"2011","journal-title":"Tumour Biol."},{"key":"10.1016\/j.abb.2016.02.025_bib24","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/1756-0500-4-3","article-title":"Comparison of different methods for DNA-free RNA isolation from SK-N-MC neuroblastoma","volume":"4","author":"Tavares","year":"2011","journal-title":"BMC Res. Notes"},{"key":"10.1016\/j.abb.2016.02.025_bib25","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1128\/AAC.50.1.88-95.2006","article-title":"Resistance of Leishmania donovani to sodium stibogluconate is related to the expression of host and parasite \u03b3-glutamylcysteine synthetase","volume":"50","author":"Carter","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.abb.2016.02.025_bib26","doi-asserted-by":"crossref","first-page":"24717","DOI":"10.1074\/jbc.M201961200","article-title":"Increase in cytosolic Ca2+ levels through the activation of non-selective cation channels induced by oxidative stress causes mitochondrial depolarization leading to apoptosis-like death in Leishmania donovani promastigotes","volume":"277","author":"Mukherjee","year":"2002","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.abb.2016.02.025_bib27","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.exppara.2005.12.020","article-title":"An intracellular calcium store is present in Leishmania donovani glycosomes","volume":"113","author":"Gupta","year":"2006","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.abb.2016.02.025_bib28","doi-asserted-by":"crossref","first-page":"11345","DOI":"10.1016\/S0021-9258(19)38598-9","article-title":"A\u00a0high affinity Ca2+ - ATPase on the surface membrane of Leishmania donovani promastigote","volume":"265","author":"Ghosh","year":"1990","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.abb.2016.02.025_bib29","doi-asserted-by":"crossref","first-page":"3214","DOI":"10.4049\/jimmunol.175.5.3214","article-title":"Leishmania donovani affects antigen presentation of macrophage by disrupting lipid rafts","volume":"175","author":"Chakraborty","year":"2005","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.abb.2016.02.025_bib30","doi-asserted-by":"crossref","first-page":"2652","DOI":"10.1016\/S0021-9258(19)42780-4","article-title":"Dynamics of the hydrogen layer in liposomes of lecithin and sphingomyelin containing dicetylphosphate","volume":"249","author":"Shinitzky","year":"1974","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.abb.2016.02.025_bib31","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.2174\/1389450115666141016150007","article-title":"Proteinase inhibitors: a promising drug class for treating leishmaniasis","volume":"15","author":"Pereira","year":"2014","journal-title":"Curr. Drug Targets"},{"key":"10.1016\/j.abb.2016.02.025_bib32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18869\/acadpub.vacres.1.1.1","article-title":"Expressional comparison between episomal and stable transfection of a selected tri-fusion protein in Leishmania tarentolae","volume":"1","author":"Taheri","year":"2014","journal-title":"Vacc R."},{"key":"10.1016\/j.abb.2016.02.025_bib33","doi-asserted-by":"crossref","first-page":"C817","DOI":"10.1152\/ajpcell.00139.2004","article-title":"Calcium, ATP, and ROS: a mitochondrial love-hate triangle","volume":"287","author":"Brookes","year":"2004","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"10.1016\/j.abb.2016.02.025_bib34","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1128\/EC.00123-07","article-title":"Characterization of metacaspases with trypsin-like activity and their putative role in programmed cell death in the protozoan parasite Leishmania","volume":"6","author":"Lee","year":"2007","journal-title":"Eukaryot. Cell."},{"key":"10.1016\/j.abb.2016.02.025_bib35","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1007\/s00436-007-0706-5","article-title":"Effects of serine protease inhibitors on viability and morphology of Leishmania (Leishmania) amazonensis promastigotes","volume":"101","author":"Silva-Lopez","year":"2007","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.abb.2016.02.025_bib36","doi-asserted-by":"crossref","first-page":"1390","DOI":"10.1038\/sj.cdd.4401661","article-title":"Cells die with increased cytosolic ATP during apoptosis: a bioluminescence study with intracellular luciferase","volume":"12","author":"Zamaraeva","year":"2005","journal-title":"Cell Death Differ."},{"key":"10.1016\/j.abb.2016.02.025_bib37","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/0022-1759(95)00072-I","article-title":"A\u00a0novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V","volume":"184","author":"Vermes","year":"1995","journal-title":"J.\u00a0Immunol. Methods"},{"key":"10.1016\/j.abb.2016.02.025_bib38","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1006\/excr.1998.4225","article-title":"Apoptosis-independent alterations in membrane dynamics induced by curcumin","volume":"245","author":"Jaruga","year":"1998","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.abb.2016.02.025_bib39","doi-asserted-by":"crossref","first-page":"444","DOI":"10.5483\/BMBRep.2008.41.6.444","article-title":"Modeled Structure of Trypanothione Reductase of Leismania infantum","volume":"41","author":"Singh","year":"2008","journal-title":"BMB Rep."},{"key":"10.1016\/j.abb.2016.02.025_bib40","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1021\/ml200280r","article-title":"Molecular docking and in\u00a0vitro antileishmanial evaluation of chromene-2-thione analogues","volume":"3","author":"Verma","year":"2012","journal-title":"ACS Med. Chem. Lett."},{"key":"10.1016\/j.abb.2016.02.025_bib41","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.ejmech.2012.04.034","article-title":"Iridoid glucosides from Nyctanthes arbortristis result in increased reactive oxygen species and cellular redox homeostasis imbalance in Leishmania parasite","volume":"54","author":"Shukla","year":"2012","journal-title":"Eur. J. Med. Chem."}],"container-title":["Archives of Biochemistry and Biophysics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003986116300443?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003986116300443?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,6,5]],"date-time":"2022-06-05T16:54:34Z","timestamp":1654448074000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003986116300443"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4]]},"references-count":41,"alternative-id":["S0003986116300443"],"URL":"https:\/\/doi.org\/10.1016\/j.abb.2016.02.025","relation":{},"ISSN":["0003-9861"],"issn-type":[{"type":"print","value":"0003-9861"}],"subject":[],"published":{"date-parts":[[2016,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Understanding the importance of conservative hypothetical protein LdBPK_070020 in Leishmania donovani and its role in subsistence of the parasite","name":"articletitle","label":"Article Title"},{"value":"Archives of Biochemistry and Biophysics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.abb.2016.02.025","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2016 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}