{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T18:40:45Z","timestamp":1758048045949,"version":"3.44.0"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100021778","name":"Agencia Nacional De Promoci\u00f3n Cient\u00edfica Y Tecnol\u00f3gica","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021778","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Archives of Biochemistry and Biophysics"],"published-print":{"date-parts":[[2023,3]]},"DOI":"10.1016\/j.abb.2023.109550","type":"journal-article","created":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T02:12:55Z","timestamp":1676340775000},"page":"109550","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Structural differences between hypoxanthine phosphoribosyltransferase family members highlight opportunities for antiparasitic drug design in neglected diseases"],"prefix":"10.1016","volume":"737","author":[{"given":"J.","family":"Santos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.H.","family":"Fern\u00e1ndez Villamil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.M.","family":"Delfino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8066-5445","authenticated-orcid":false,"given":"W.M.","family":"Valsecchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.abb.2023.109550_bib1","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/S0959-440X(01)00274-3","article-title":"The PRT protein family","volume":"11","author":"Sinha","year":"2001","journal-title":"Curr. Opin. Struct. Biol."},{"key":"10.1016\/j.abb.2023.109550_bib2","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/0002-9343(64)90104-4","article-title":"A familial disorder of uric acid metabolism and central nervous system function","volume":"36","author":"Lesch","year":"1964","journal-title":"Am. J. Med."},{"key":"10.1016\/j.abb.2023.109550_bib3","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S0022-3476(65)80249-9","article-title":"A familial disorder of uric acid metabolism and central nervous system function. II","author":"Nyhan","year":"1965","journal-title":"J. Pediatr."},{"key":"10.1016\/j.abb.2023.109550_bib4","doi-asserted-by":"crossref","first-page":"155","DOI":"10.7326\/0003-4819-70-1-155","article-title":"Hypoxanthine-Guanine phosphoribosyltransferase deficiency in gout","volume":"70","author":"Kelley","year":"1968","journal-title":"Ann. Intern. Med."},{"key":"10.1016\/j.abb.2023.109550_bib5","doi-asserted-by":"crossref","first-page":"16084","DOI":"10.1074\/jbc.M600188200","article-title":"A conditional mutant deficient in hypoxanthine-guanine phosphoribosyltransferase and xanthine phosphoribosyltransferase validates the purine salvage pathway of Leishmania donovani","volume":"281","author":"Boitz","year":"2006","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.abb.2023.109550_bib6","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/0166-6851(93)90039-Z","article-title":"Inhibition of Plasmodium falciparum hypoxanthine-guanine phosphoribosyltransferase mRNA by antisense oligodeoxynucleotide sequence","volume":"60","author":"Dawson","year":"1993","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.abb.2023.109550_bib7","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1016\/S1074-5521(00)00045-4","article-title":"Efficient identification of inhibitors targeting the closed active site conformation of the HPRT from Trypanosoma cruzi","volume":"7","author":"Freymann","year":"2000","journal-title":"Chem. Biol."},{"key":"10.1016\/j.abb.2023.109550_bib8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.ppat.1002251","article-title":"High-Resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism","volume":"7","author":"Griffin","year":"2011","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.abb.2023.109550_bib9","doi-asserted-by":"crossref","first-page":"40706","DOI":"10.1074\/jbc.M111.291138","article-title":"Structural analyses of a purine biosynthetic enzyme from Mycobacterium tuberculosis reveal a novel bound nucleotide","volume":"286","author":"le Nours","year":"2011","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.abb.2023.109550_bib10","doi-asserted-by":"crossref","first-page":"14485","DOI":"10.1021\/bi990507q","article-title":"Crystal structures of the Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase-GMP and -IMP complexes: comparison of purine binding interactions with the XMP complex","volume":"38","author":"H\u00e9roux","year":"1999","journal-title":"Biochemistry"},{"key":"10.1016\/j.abb.2023.109550_bib11","doi-asserted-by":"crossref","first-page":"1900","DOI":"10.1111\/j.1742-4658.2005.04620.x","article-title":"Plasmodium falciparum hypoxanthine guanine phosphoribosyltransferase. Stability studies on the product-activated enzyme","volume":"272","author":"Raman","year":"2005","journal-title":"FEBS J."},{"key":"10.1016\/j.abb.2023.109550_bib12","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1038\/9376","article-title":"The 2.0 A structure of human hypoxanthine-guanine phosphoribosyltransferase in complex with a transition-state analog inhibitor","volume":"6","author":"Shi","year":"1999","journal-title":"Nat. Struct. Mol. Biol."},{"key":"10.1016\/j.abb.2023.109550_bib13","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/j.bbapap.2016.03.005","article-title":"The role of the C-terminal region on the oligomeric state and enzymatic activity of Trypanosoma cruzi hypoxanthine phosphoribosyl transferase","volume":"1864","author":"Valsecchi","year":"2016","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.abb.2023.109550_bib14","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0167-4838(02)00334-5","article-title":"Cloning, characterization and preliminary crystallographic analysis of Leishmania hypoxanthine\u2013guanine phosphoribosyltransferase","volume":"1598","author":"Monzani","year":"2002","journal-title":"Biochemica et Biophysica Acta"},{"key":"10.1016\/j.abb.2023.109550_bib15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1472-6807-7-59","article-title":"Crystal structure of Leishmania tarentolae hypoxanthine-guanine phosphoribosyltransferase","volume":"7","author":"Monzani","year":"2007","journal-title":"BMC Struct. Biol."},{"key":"10.1016\/j.abb.2023.109550_bib16","doi-asserted-by":"crossref","first-page":"15055","DOI":"10.1021\/bi981052s","article-title":"A 1.4 \u00c5 crystal structure for the hypoxanthine phosphoribosyltransferase of trypanosoma cruzi","volume":"37","author":"Focia","year":"1998","journal-title":"Biochemistry"},{"key":"10.1016\/j.abb.2023.109550_bib17","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1002\/pro.2098","article-title":"Functional significance of four successive glycine residues in the pyrophosphate binding loop of fungal 6-oxopurine phosphoribosyltransferases","volume":"21","author":"Moyni\u00e9","year":"2012","journal-title":"Protein Sci."},{"key":"10.1016\/j.abb.2023.109550_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.bcp.2021.114524","article-title":"Zoledronate repositioning as a potential trypanocidal drug. Trypanosoma cruzi HPRT an alternative target to be considered","volume":"188","author":"Valsecchi","year":"2021","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.abb.2023.109550_bib19","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.bbrc.2013.10.029","article-title":"HIV-1 TAT-mediated protein transduction of human HPRT into deficient cells","volume":"441","author":"Cattelan","year":"2013","journal-title":"Biochem. Biophys. Res. Commun."},{"year":"1997","series-title":"Kinetic Mechanism of Human Hypoxanthine-Guanine Phosphoribosyltransferase","author":"Xu","key":"10.1016\/j.abb.2023.109550_bib20"},{"year":"1992","series-title":"Purine Nucleoside Phosphorylase. Inosine Hydrolysis, Tight Binding of the Hypoxanthine Intermediate, and Third-The-Sites Reactivity?","author":"Kline","key":"10.1016\/j.abb.2023.109550_bib21"},{"key":"10.1016\/j.abb.2023.109550_bib22","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.jmb.2005.05.061","article-title":"The crystal structure of free human hypoxanthine-guanine phosphoribosyltransferase reveals extensive conformational plasticity throughout the catalytic cycle","volume":"351","author":"Keough","year":"2005","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.abb.2023.109550_bib23","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkm363","article-title":"PrDOS: prediction of disordered protein regions from amino acid sequence","volume":"35","author":"Ishida","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.abb.2023.109550_bib24","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/978-1-4939-0366-5_10","article-title":"POODLE: tools predicting intrinsically disordered regions of amino acid sequence","volume":"1137","author":"Shimizu","year":"2014","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.abb.2023.109550_bib25","doi-asserted-by":"crossref","first-page":"3433","DOI":"10.1093\/bioinformatics\/bti541","article-title":"IUPred:web server for the prediction of intrinsically unstructured regions of proteins based on estimated energy content","volume":"21","author":"Dosztanyi","year":"2005","journal-title":"Bioinformatics"},{"key":"10.1016\/j.abb.2023.109550_bib26","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1093\/nar\/gkl893","article-title":"DisProt: the database of disordered proteins","volume":"35","author":"Sickmeier","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.abb.2023.109550_bib27","article-title":"MobiDB: 10 years of intrinsically disordered proteins","author":"Piovesan","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.abb.2023.109550_bib28","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gki387","article-title":"The FoldX web server: an online force field","volume":"33","author":"Schymkowitz","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.abb.2023.109550_bib29","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkm423","article-title":"PIC: protein interactions calculator","volume":"35","author":"Tina","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.abb.2023.109550_bib30","doi-asserted-by":"crossref","first-page":"W338","DOI":"10.1093\/nar\/gky356","article-title":"CABS-flex 2.0: a web server for fast simulations of flexibility of protein structures","volume":"46","author":"Kuriata","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.abb.2023.109550_bib31","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-82410-1","article-title":"New machine learning and physics-based scoring functions for drug discovery","volume":"11","author":"Guedes","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.abb.2023.109550_bib32","doi-asserted-by":"crossref","first-page":"2981","DOI":"10.1093\/bioinformatics\/btu426","article-title":"YASARA View - molecular graphics for all devices - from smartphones to workstations","volume":"30","author":"Krieger","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.abb.2023.109550_bib33","doi-asserted-by":"crossref","first-page":"4501","DOI":"10.1016\/j.bmcl.2004.06.042","article-title":"The purine transferase from Trypanosoma cruzi as a potential target for bisphosphonate-based chemotherapeutic compounds","volume":"14","author":"Fern\u00e1ndez","year":"2004","journal-title":"Bioorg. Med. Chem. Lett"},{"key":"10.1016\/j.abb.2023.109550_bib34","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/0166-6851(94)90075-2","article-title":"Molecular characterization and overexpression of the hypoxanthine-guanine phosphoribosyltransferase gene from Trypanosoma cruzi","volume":"65","author":"Allen","year":"1994","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.abb.2023.109550_bib35","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1016\/S0167-4838(98)00210-6","article-title":"Limited proteolysis of a trypanosomal hypoxanthine phosphoribosyltransferase yields crystals that difract X-rays to near atomic resolution","volume":"1388","author":"Nieves-Alicea","year":"1998","journal-title":"Biochemica et Biophysica Acta"},{"key":"10.1016\/j.abb.2023.109550_bib36","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.biochi.2016.12.020","article-title":"Oligomeric state of hypoxanthine guanine phosphoribosyltransferase from Mycobacterium tuberculosis","volume":"135","author":"Eng","year":"2017","journal-title":"Biochimie"},{"key":"10.1016\/j.abb.2023.109550_bib37","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0166-6851(98)00139-X","article-title":"J. de Jersey, Purification and characterization of Plasmodium falciparum hypoxanthine\u2013guanine\u2013xanthine phosphoribosyltransferase and comparison with the human enzyme","volume":"98","author":"Keough","year":"1998","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.abb.2023.109550_bib38","doi-asserted-by":"crossref","first-page":"20231","DOI":"10.1074\/jbc.R000002200","article-title":"Purine phosphoribosyltransferases","volume":"275","author":"Craig","year":"2000","journal-title":"J. Biol. Chem."}],"container-title":["Archives of Biochemistry and Biophysics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003986123000498?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003986123000498?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T06:22:44Z","timestamp":1757744564000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003986123000498"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3]]},"references-count":38,"alternative-id":["S0003986123000498"],"URL":"https:\/\/doi.org\/10.1016\/j.abb.2023.109550","relation":{},"ISSN":["0003-9861"],"issn-type":[{"type":"print","value":"0003-9861"}],"subject":[],"published":{"date-parts":[[2023,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Structural differences between hypoxanthine phosphoribosyltransferase family members highlight opportunities for antiparasitic drug design in neglected diseases","name":"articletitle","label":"Article Title"},{"value":"Archives of Biochemistry and Biophysics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.abb.2023.109550","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"109550"}}