{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T16:30:22Z","timestamp":1782577822436,"version":"3.54.5"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001409","name":"Department of Science and Technology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["1164"],"award-info":[{"award-number":["1164"]}],"id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001409","name":"Department of Science and Technology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["IF180283"],"award-info":[{"award-number":["IF180283"]}],"id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Archives of Biochemistry and Biophysics"],"published-print":{"date-parts":[[2025,3]]},"DOI":"10.1016\/j.abb.2025.110303","type":"journal-article","created":{"date-parts":[[2025,1,11]],"date-time":"2025-01-11T07:20:30Z","timestamp":1736580030000},"page":"110303","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Gymnema saponin-induced lipid flip-flop identifies rigid membrane phenotype of methicillin resistant S. aureus and enhances it's antibiotic susceptibility"],"prefix":"10.1016","volume":"765","author":[{"given":"Gayatree","family":"Panda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swagatika","family":"Dehury","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Himadri Gourav","family":"Behuria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bijesh Kumar","family":"Biswal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashis Kumar","family":"Jena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indrani","family":"Mohanty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sasmita","family":"Hotta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santosh Kumar","family":"Padhi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5141-8103","authenticated-orcid":false,"given":"Santosh Kumar","family":"Sahu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.abb.2025.110303_bib1","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1086\/341454","article-title":"Staphylococcus aureus strains lacking D-alanine modifications of teichoic acids are highly susceptible to human neutrophil killing and are virulence attenuated in mice","volume":"186","author":"Collins","year":"2002","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.abb.2025.110303_bib2","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.peptides.2012.05.025","article-title":"Characterization of cell membrane parameters of clinical isolates of Staphylococcus aureus with varied susceptibility to alpha-melanocyte stimulating hormone","volume":"37","author":"Shireen","year":"2012","journal-title":"Peptides"},{"key":"10.1016\/j.abb.2025.110303_bib3","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1016\/j.bbalip.2016.11.013","article-title":"Bacterial aminoacyl phospholipids - biosynthesis and role in basic cellular processes and pathogenicity","volume":"1862","author":"Slavetinsky","year":"2017","journal-title":"Biochim. Biophys. Acta, Mol. Cell Biol. Lipids"},{"issue":"2","key":"10.1016\/j.abb.2025.110303_bib4","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1111\/j.1365-2958.2011.07576.x","article-title":"Broad spectrum antimicrobial peptide resistance by MprF mediated aminoacylation and flipping of phospholipids","volume":"80","author":"Ernst","year":"2011","journal-title":"Mol. Microbiol."},{"issue":"6","key":"10.1016\/j.abb.2025.110303_bib5","doi-asserted-by":"crossref","first-page":"1354","DOI":"10.1016\/j.cell.2017.10.012","article-title":"Membrane microdomain disassembly inhibits MRSA antibiotic resistance","volume":"171","author":"Garc\u00eda-Fern\u00e1ndez","year":"2017","journal-title":"Cell"},{"key":"10.1016\/j.abb.2025.110303_bib6","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1128\/AAC.00680-10","article-title":"Carotenoid-related alteration of cell membrane fluidity impacts Staphylococcus aureus susceptibility to host defence peptides","volume":"55","author":"Mishra","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.abb.2025.110303_bib7","doi-asserted-by":"crossref","first-page":"350","DOI":"10.3389\/fcell.2020.00350","article-title":"Localization preference of antimicrobial peptides on liquid-disordered membrane domains","volume":"8","author":"Su","year":"2020","journal-title":"Front. Cell Dev. Biol."},{"issue":"10","key":"10.1016\/j.abb.2025.110303_bib8","doi-asserted-by":"crossref","DOI":"10.1128\/aac.00051-23","article-title":"A small-molecule membrane fluidizer re-sensitizes methicillin-resistant Staphylococcus aureus (MRSA) to \u03b2-lactam antibiotics","volume":"67","author":"Podoll","year":"2023","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.abb.2025.110303_bib9","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1007\/s12010-020-03399-3","article-title":"An anti-microbial Terpenoid fraction from Gymnema sylvestre induces flip-flop of fluorescent-phospholipid analogs in model membrane","volume":"192","author":"Behuria","year":"2020","journal-title":"Appl. Biochem. Biotechnol."},{"issue":"51","key":"10.1016\/j.abb.2025.110303_bib10","doi-asserted-by":"crossref","first-page":"35667","DOI":"10.1021\/acsomega.1c05581","article-title":"Lipid flip-flop-inducing antimicrobial phytochemicals from Gymnema sylvestre are bacterial membrane permeability enhancers","volume":"6","author":"Behuria","year":"2021","journal-title":"ACS Omega"},{"key":"10.1016\/j.abb.2025.110303_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2023.102512","article-title":"Antimicrobial susceptibility testing to evaluate minimum inhibitory concentration values of clinically relevant antibiotics","volume":"4","author":"Barnes","year":"2023","journal-title":"STAR protocols"},{"issue":"5","key":"10.1016\/j.abb.2025.110303_bib12","doi-asserted-by":"crossref","first-page":"443","DOI":"10.4103\/0250-474X.108420","article-title":"Phytochemical screening and antimicrobial activity of some medicinal plants against multi-drug resistant bacteria from clinical isolates","volume":"74","author":"Dahiya","year":"2012","journal-title":"Indian J. Pharmaceut. Sci."},{"issue":"8","key":"10.1016\/j.abb.2025.110303_bib13","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/o59-099","article-title":"A rapid method for total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can. J. Biochem. Physiol."},{"issue":"3","key":"10.1016\/j.abb.2025.110303_bib14","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1080\/08982104.2020.1800729","article-title":"Electroformation of liposomes and phytosomes using copper electrode","volume":"31","author":"Behuria","year":"2021","journal-title":"J. Liposome Res."},{"issue":"39","key":"10.1016\/j.abb.2025.110303_bib15","doi-asserted-by":"crossref","first-page":"10481","DOI":"10.1021\/bi8014339","article-title":"Flippase activity in proteoliposomes reconstituted with Spinacea oleracea endoplasmic reticulum membrane proteins: evidence of biogenic membrane flippase in plants","volume":"47","author":"Sahu","year":"2008","journal-title":"Biochemistry"},{"key":"10.1016\/j.abb.2025.110303_bib16","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/j.colsurfb.2019.06.027","article-title":"Rapid formation of small unilamellar vesicles (SUV) through low-frequency sonication: an innovative approach","volume":"181","author":"de Freitas","year":"2019","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"10.1016\/j.abb.2025.110303_bib17","first-page":"4025","article-title":"Fusion between fluid liposomes and intact bacteria: study of driving parameters and in vitro bactericidal efficacy","volume":"17","author":"Wang","year":"2016","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.abb.2025.110303_bib18","doi-asserted-by":"crossref","first-page":"93","DOI":"10.3389\/fnmol.2017.00093","article-title":"Visualization of SNARE-mediated hemifusion between giant unilamellar vesicles arrested by myricetin","volume":"10","author":"Heo","year":"2017","journal-title":"Front. Mol. Neurosci."},{"key":"10.1016\/j.abb.2025.110303_bib19","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.jep.2010.04.025","article-title":"Eugenol (an essential oil of clove) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane","volume":"130","author":"Devi","year":"2010","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.abb.2025.110303_bib20","doi-asserted-by":"crossref","first-page":"3132","DOI":"10.1128\/AAC.00124-10","article-title":"Damage of the bacterial cell envelope by antimicrobial peptides gramicidin S and PGLa as revealed by transmission and scanning electron microscopy","volume":"54","author":"Hartmann","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.abb.2025.110303_bib21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000660","article-title":"The bacterial defensin resistance protein MprF consists of separable domains for lipid lysinylation and antimicrobial peptide repulsion","volume":"5","author":"Ernst","year":"2009","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.abb.2025.110303_bib22","series-title":"Alteration of Zeta Potential and Membrane Permeability in Bacteria: a Study with Cationic Agents","first-page":"1","author":"Halder","year":"2015"},{"key":"10.1016\/j.abb.2025.110303_bib23","doi-asserted-by":"crossref","first-page":"6193","DOI":"10.1038\/s41467-021-26517-z","article-title":"The cell envelope of Staphylococcus aureus selectively controls the sorting of virulence factors","volume":"12","author":"Zheng","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.abb.2025.110303_bib24","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1093\/nar\/gku340","article-title":"SWISS-MODEL: modelling protein tertiary and quaternary structure using evolutionary information","volume":"42","author":"Biasini","year":"2014","journal-title":"Nucleic Acids Res."},{"issue":"41","key":"10.1016\/j.abb.2025.110303_bib25","article-title":"ChemDraw Ultra 10.0 cambridge soft, 100 cambridge park drive","volume":"128","author":"Mills","year":"2006","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.abb.2025.110303_bib26","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1007\/s10822-010-9352-6","article-title":"Ligand docking and binding site analysis with PyMOL and Autodock\/Vina","volume":"24","author":"Seeliger","year":"2010","journal-title":"J. Comput. Aided Mol. Des."},{"key":"10.1016\/j.abb.2025.110303_bib27","doi-asserted-by":"crossref","first-page":"13","DOI":"10.3389\/fmicb.2016.00013","article-title":"Phenotypic modifications in Staphylococcus aureus cells exposed to high concentrations of vancomycin and teicoplanin","volume":"7","author":"Gon\u00e7alves","year":"2016","journal-title":"Front. Microbiol."},{"issue":"7811","key":"10.1016\/j.abb.2025.110303_bib28","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1038\/s41586-020-2236-6","article-title":"The architecture of the Gram-positive bacterial cell wall","volume":"582","author":"Pasquina-Lemonche","year":"2020","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.abb.2025.110303_bib29","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.chembiol.2012.11.006","article-title":"Structural properties of model phosphatidylcholine flippases","volume":"20","author":"Langer","year":"2013","journal-title":"Chem. Biol."},{"issue":"10","key":"10.1016\/j.abb.2025.110303_bib30","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1016\/j.bpj.2023.04.018","article-title":"High curvature promotes fusion of lipid membranes: predictions from continuum elastic theory","volume":"122","author":"Golani","year":"2023","journal-title":"Biophys. J."},{"key":"10.1016\/j.abb.2025.110303_bib31","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.colsurfb.2012.04.024","article-title":"Lipid exchange between membranes: effects of membrane surface charge, composition, and curvature","volume":"97","author":"Zhu","year":"2012","journal-title":"Colloids Surf. B Biointerfaces"},{"issue":"7","key":"10.1016\/j.abb.2025.110303_bib32","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1016\/j.bbamem.2018.04.007","article-title":"Lipid flip-flop vs. lateral diffusion in the relaxation of hemifusion diaphragms","volume":"1860","author":"Gardner","year":"2018","journal-title":"Biochim. Biophys. Acta Biomembr."},{"issue":"3","key":"10.1016\/j.abb.2025.110303_bib33","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S0169-409X(99)00030-7","article-title":"Lipid vesicles and membrane fusion","volume":"38","author":"Cevc","year":"1999","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.abb.2025.110303_bib34","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0158457","article-title":"FRAP to characterize molecular diffusion and interaction in various membrane environments","volume":"11","author":"Pincet","year":"2016","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.abb.2025.110303_bib35","doi-asserted-by":"crossref","DOI":"10.1038\/srep29369","article-title":"Micro-motors: a motile bacteria based system for liposome cargo transport","volume":"6","author":"Dogra","year":"2016","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.abb.2025.110303_bib36","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1038\/s41467-022-29272-x","article-title":"Membrane thickness, lipid phase and sterol type are determining factors in the permeability of membranes to small solutes","volume":"13","author":"Frallicciardi","year":"2022","journal-title":"Nat. Commun."},{"issue":"10","key":"10.1016\/j.abb.2025.110303_bib37","first-page":"1735","volume":"122","author":"Blake","year":"2023","journal-title":"Facilitating flip-flop: Structural tuning of molecule-membrane interactions in living bacteria"},{"issue":"1","key":"10.1016\/j.abb.2025.110303_bib38","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1038\/s41392-024-01866-5","article-title":"Bacterial persisters: molecular mechanisms and therapeutic development","volume":"9","author":"Niu","year":"2024","journal-title":"Signal Transduct. Targeted Ther."},{"key":"10.1016\/j.abb.2025.110303_bib39","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1002\/ptr.4891","article-title":"Effect of corilagin on membrane permeability of Escherichia coli, Staphylococcus aureus and Candida albicans","volume":"27","author":"Li","year":"2013","journal-title":"Phytother Res."},{"key":"10.1016\/j.abb.2025.110303_bib40","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1038\/s41598-020-79713-0","article-title":"Carvacrol exhibits rapid bactericidal activity against Streptococcus pyogenes through cell membrane damage","volume":"11","author":"Wijesundara","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.abb.2025.110303_bib41","doi-asserted-by":"crossref","first-page":"3408","DOI":"10.1039\/D3SC04523D","article-title":"The intricate link between membrane lipid structure and composition and membrane structural properties in bacterial membranes","volume":"15","author":"Lee","year":"2024","journal-title":"Chem. Sci."},{"key":"10.1016\/j.abb.2025.110303_bib42","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s12551-021-00912-8","article-title":"Lipid-mediated antimicrobial resistance: a phantom menace or a new hope?","volume":"14","author":"MacDermott-Opeskin","year":"2022","journal-title":"Biophys. Rev."},{"key":"10.1016\/j.abb.2025.110303_bib43","doi-asserted-by":"crossref","first-page":"2927","DOI":"10.1038\/s41467-021-23248-z","article-title":"Phospholipid translocation captured in a bifunctional membrane protein MprF","volume":"12","author":"Song","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.abb.2025.110303_bib44","doi-asserted-by":"crossref","first-page":"1788","DOI":"10.1021\/cb500086e","article-title":"Phytochemicals perturb membranes and promiscuously alter protein function","volume":"9","author":"Ing\u00f3lfsson","year":"2014","journal-title":"ACS Chem. Biol."},{"key":"10.1016\/j.abb.2025.110303_bib45","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.66376","article-title":"Sensitizing Staphylococcus aureus to antibacterial agents by decoding and blocking the lipid flippase MprF","volume":"11","author":"Slavetinsky","year":"2022","journal-title":"Elife"},{"key":"10.1016\/j.abb.2025.110303_bib46","doi-asserted-by":"crossref","first-page":"3492","DOI":"10.1128\/AAC.00370-12","article-title":"Alanyl-phosphatidylglycerol and lysyl-phosphatidylglycerol are translocated by the same MprF flippases and have similar capacities to protect against the antibiotic daptomycin in Staphylococcus aureus","volume":"56","author":"Slavetinsky","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"issue":"11","key":"10.1016\/j.abb.2025.110303_bib47","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000660","article-title":"The bacterial defensin resistance protein MprF consists of separable domains for lipid lysinylation and antimicrobial peptide repulsion","volume":"5","author":"Ernst","year":"2009","journal-title":"PLoS Pathog."},{"issue":"10325","key":"10.1016\/j.abb.2025.110303_bib48","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis","volume":"399","author":"Murray","year":"2022","journal-title":"Lancet"},{"issue":"1","key":"10.1016\/j.abb.2025.110303_bib49","doi-asserted-by":"crossref","first-page":"57","DOI":"10.3184\/003685002783238870","article-title":"Methicillin resistance in Staphylococcus aureus: mechanisms and modulation","volume":"85","author":"Stapleton","year":"2002","journal-title":"Sci. Prog."}],"container-title":["Archives of Biochemistry and Biophysics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003986125000165?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003986125000165?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T08:09:14Z","timestamp":1746000554000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003986125000165"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":49,"alternative-id":["S0003986125000165"],"URL":"https:\/\/doi.org\/10.1016\/j.abb.2025.110303","relation":{},"ISSN":["0003-9861"],"issn-type":[{"value":"0003-9861","type":"print"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Gymnema saponin-induced lipid flip-flop identifies rigid membrane phenotype of methicillin resistant S. aureus and enhances it's antibiotic susceptibility","name":"articletitle","label":"Article Title"},{"value":"Archives of Biochemistry and Biophysics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.abb.2025.110303","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"110303"}}