{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T02:42:36Z","timestamp":1776134556252,"version":"3.50.1"},"reference-count":37,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,12,11]],"date-time":"2021-12-11T00:00:00Z","timestamp":1639180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2010\/51729-2"],"award-info":[{"award-number":["2010\/51729-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008353","name":"Funda\u00e7\u00e3o de Apoio ao Ensino, Pesquisa e Assist\u00eancia do Hospital das Cl\u00ednicas da Faculdade de Medicina de Ribeir\u00e3o Preto da Universidade de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2016\/09011-3"],"award-info":[{"award-number":["2016\/09011-3"]}],"id":[{"id":"10.13039\/501100008353","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Anais Brasileiros de Dermatologia"],"published-print":{"date-parts":[[2022,3]]},"DOI":"10.1016\/j.abd.2021.06.004","type":"journal-article","created":{"date-parts":[[2022,1,17]],"date-time":"2022-01-17T15:57:41Z","timestamp":1642435061000},"page":"145-156","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"title":["Autoantibodies against desmoglein 2 are not pathogenic in pemphigus"],"prefix":"10.1016","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8820-016X","authenticated-orcid":false,"given":"Marcela Calixto Brand\u00e3o","family":"Miguel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0536-9726","authenticated-orcid":false,"given":"Tamiris Amanda","family":"Julio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8589-0089","authenticated-orcid":false,"given":"Sebastian","family":"Vernal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3744-8947","authenticated-orcid":false,"given":"Nat\u00e1lia Aparecida","family":"de Paula","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6742-6266","authenticated-orcid":false,"given":"Andre","family":"Lieber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2709-1825","authenticated-orcid":false,"given":"Ana Maria","family":"Roselino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.abd.2021.06.004_bib0005","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1016\/S0140-6736(19)31778-7","article-title":"Pemphigus","volume":"394","author":"Schmidt","year":"2019","journal-title":"Lancet"},{"key":"10.1016\/j.abd.2021.06.004_bib0010","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1016\/j.jaci.2020.02.013","article-title":"Autoimmune bullous skin diseases, pemphigus and pemphigoid","volume":"145","author":"Egami","year":"2020","journal-title":"J Allergy Clin Immunol"},{"key":"10.1016\/j.abd.2021.06.004_bib0015","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.4269\/ajtmh.17-0100","article-title":"Spatial Distribution of Pemphigus Occurrence over Five Decades in Southeastern Brazil","volume":"97","author":"Celere","year":"2017","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.abd.2021.06.004_bib0020","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.jaut.2016.04.007","article-title":"Differential HLA class I and class II associations in pemphigus foliaceus and pemphigus vulgaris patients from a prevalent Southeastern Brazilian region","volume":"72","author":"Brochado","year":"2016","journal-title":"J Autoimmun"},{"key":"10.1016\/j.abd.2021.06.004_bib0025","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1006\/excr.1994.1103","article-title":"Identification of the Ubiquitous Human Desmoglein, Dsg2, and the Expression Catalogue of the Desmoglein Subfamily of Desmosomal Cadherins","volume":"211","author":"Sch\u00e4fer","year":"1994","journal-title":"Exp Cell Res"},{"key":"10.1016\/j.abd.2021.06.004_bib0030","doi-asserted-by":"crossref","first-page":"53739","DOI":"10.1371\/journal.pone.0053739","article-title":"Desmoglein 2 is less important than desmoglein 3 for keratinocyte cohesion","volume":"8","author":"Hartlieb","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.abd.2021.06.004_bib0035","doi-asserted-by":"crossref","first-page":"10841","DOI":"10.1128\/JVI.01425-15","article-title":"Intracellular Signaling and Desmoglein 2 Shedding Triggered by Human Adenoviruses Ad3, Ad14, and Ad14P1","volume":"89","author":"Wang","year":"2015","journal-title":"J Virol"},{"key":"10.1016\/j.abd.2021.06.004_bib0040","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1038\/jid.2011.390","article-title":"Desmoglein as a target in skin disease and beyond","volume":"132","author":"Amagai","year":"2012","journal-title":"J Invest Dermatol"},{"key":"10.1016\/j.abd.2021.06.004_bib0045","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1007\/s00432-016-2250-0","article-title":"Desmoglein-2 is overexpressed in non-small cell lung cancer tissues and its knockdown suppresses NSCLC growth by regulation of p27 and CDK2","volume":"143","author":"Cai","year":"2017","journal-title":"J Cancer Res Clin Oncol"},{"key":"10.1016\/j.abd.2021.06.004_bib0050","doi-asserted-by":"crossref","first-page":"1154","DOI":"10.1080\/15384047.2020.1843323","article-title":"Desmoglein-2 as a prognostic and biomarker in ovarian cancer","volume":"21","author":"Kim","year":"2020","journal-title":"Cancer Biol Ther"},{"key":"10.1016\/j.abd.2021.06.004_bib0055","doi-asserted-by":"crossref","first-page":"148","DOI":"10.4161\/cam.3.2.7539","article-title":"Increased expression of Dsg2 in malignant skin carcinomas: A tissue-microarray based study","volume":"3","author":"Brennan","year":"2009","journal-title":"Cell Adh Migr"},{"key":"10.1016\/j.abd.2021.06.004_bib0060","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1006\/excr.1997.3890","article-title":"The expression of desmoglein isoforms in cultured human keratinocytes is regulated by calcium, serum, and protein kinase C","volume":"239","author":"Denning","year":"1998","journal-title":"Exp Cell Res"},{"key":"10.1016\/j.abd.2021.06.004_bib0065","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1046\/j.1365-2133.1999.02604.x","article-title":"Internalization of constitutive desmogleins with the subsequent induction of desmoglein 2 in pemphigus lesions","volume":"140","author":"Iwatsuki","year":"1999","journal-title":"Br J Dermatol"},{"key":"10.1016\/j.abd.2021.06.004_bib0070","doi-asserted-by":"crossref","first-page":"1629","DOI":"10.2353\/ajpath.2009.080778","article-title":"The role of desmoglein-3 in the diagnosis of squamous cell carcinoma of the lung","volume":"174","author":"Savci-heijink","year":"2009","journal-title":"Am J Pathol"},{"key":"10.1016\/j.abd.2021.06.004_bib0075","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1186\/1471-2407-10-240","article-title":"Identification of valid reference genes for gene expression studies of human stomach cancer by reverse transcription-qPCR","volume":"10","author":"Rho","year":"2010","journal-title":"BMC Cancer"},{"key":"10.1016\/j.abd.2021.06.004_bib0080","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real- time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.abd.2021.06.004_bib0085","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.1038\/jid.2012.232","article-title":"IgG autoantibody response against keratinocyte cadherins in endemic pemphigus foliaceus (fogo selvagem)","volume":"132","author":"Flores","year":"2012","journal-title":"J Invest Dermatol"},{"key":"10.1016\/j.abd.2021.06.004_bib0090","doi-asserted-by":"crossref","first-page":"779","DOI":"10.3389\/fimmu.2018.00779","article-title":"Humoral Epitope Spreading in Autoimmune Bullous Diseases","volume":"9","author":"Didona","year":"2018","journal-title":"Front Immunol"},{"key":"10.1016\/j.abd.2021.06.004_bib0095","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.7754\/Clin.Lab.2015.150628","article-title":"Anti-Desmoglein 1 and 3 Autoantibody Levels in Endemic Pemphigus Foliaceus and Pemphigus Vulgaris from Brazil","volume":"62","author":"Oliveira","year":"2016","journal-title":"Clin Lab"},{"key":"10.1016\/j.abd.2021.06.004_bib0100","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1056\/NEJM200007063430104","article-title":"The prevalence of antibodies against desmoglein 1 in endemic pmphigus foliaceus in Brazil. Cooperative Group on Fogo Selvagem Research","volume":"343","author":"Warren","year":"2000","journal-title":"N Engl J Med"},{"key":"10.1016\/j.abd.2021.06.004_bib0105","doi-asserted-by":"crossref","first-page":"2044","DOI":"10.1038\/sj.jid.5700388","article-title":"Prevalence of anti-desmoglein-3 antibodies in endemic regions of Fogo selvagem in Brazil","volume":"126","author":"Hilario-Vargas","year":"2006","journal-title":"J Invest Dermatol"},{"key":"10.1016\/j.abd.2021.06.004_bib0110","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1046\/j.1365-2133.2001.04132.x","article-title":"The severity of cutaneous and oral pemphigus is related to desmoglein 1 and 3 antibody levels","volume":"144","author":"Harman","year":"2001","journal-title":"Br J Dermatol"},{"key":"10.1016\/j.abd.2021.06.004_bib0115","doi-asserted-by":"crossref","DOI":"10.1155\/2009\/187864","article-title":"Detection of autoantibodies against recombinant desmoglein 1 and 3 molecules in patients with pemphigus vulgaris: correlation with disease extent at the time of diagnosis and during follow-up","volume":"2009","author":"Belloni-Fortina","year":"2009","journal-title":"Clin Dev Immunol"},{"key":"10.1016\/j.abd.2021.06.004_bib0120","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1055\/s-2007-1004283","article-title":"Esophageal Nikolsky\u2019s sign in pemphigus vulgaris","volume":"29","author":"Coelho","year":"1997","journal-title":"Endoscopy"},{"key":"10.1016\/j.abd.2021.06.004_bib0125","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1111\/j.1365-2133.1993.tb00192.x","article-title":"An immunohistological study of desmosomal components in pemphigus","volume":"128","author":"Burge","year":"1993","journal-title":"Br J Dermatol"},{"key":"10.1016\/j.abd.2021.06.004_bib0130","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1001\/archderm.1993.01680260066008","article-title":"Autoimmune pemphigus. A distinct staining pattern with an anti-desmoglein antibody","volume":"129","author":"Carlotti","year":"1993","journal-title":"Arch Dermatol"},{"key":"10.1016\/j.abd.2021.06.004_bib0135","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1177\/104063870201400110","article-title":"Altered immunohistochemical staining for desmoglein in skin biopsies in canine pemphigus foliaceus","volume":"14","author":"Steeves","year":"2002","journal-title":"J Vet Diagn Invest"},{"key":"10.1016\/j.abd.2021.06.004_bib0140","doi-asserted-by":"crossref","first-page":"2635","DOI":"10.3389\/fimmu.2019.02635","article-title":"Endocytosis of IgG, Desmoglein 1, and Plakoglobin in Pemphigus Foliaceus Patient Skin","volume":"10","author":"Oktarina","year":"2019","journal-title":"Front Immunol"},{"key":"10.1016\/j.abd.2021.06.004_bib0145","doi-asserted-by":"crossref","first-page":"7623","DOI":"10.1074\/jbc.M512447200","article-title":"Desmoglein endocytosis and desmosome disassembly are coordinated responses to pemphigus autoantibodies","volume":"281","author":"Calkins","year":"2006","journal-title":"J Biol Chem"},{"key":"10.1016\/j.abd.2021.06.004_bib0150","doi-asserted-by":"crossref","first-page":"18303","DOI":"10.1074\/jbc.M710046200","article-title":"Pemphigus vulgaris IgG-induced desmoglein-3 endocytosis and desmosomal disassembly are mediated by a clathrin- and dynamin-independent mechanism","volume":"283","author":"Delva","year":"2008","journal-title":"J Biol Chem"},{"key":"10.1016\/j.abd.2021.06.004_bib0155","doi-asserted-by":"crossref","first-page":"8936","DOI":"10.1074\/jbc.M109.087999","article-title":"p38MAPK signaling and desmoglein-3 internalization are linked events in pemphigus acantholysis","volume":"285","author":"Jolly","year":"2010","journal-title":"J Biol Chem."},{"key":"10.1016\/j.abd.2021.06.004_bib0160","doi-asserted-by":"crossref","first-page":"858","DOI":"10.3389\/fimmu.2018.00858","article-title":"Keratin retraction and Desmoglein3 internalization independently contribute to Autoantibody-Induced Cell Dissociation in Pemphigus Vulgaris","volume":"9","author":"Schl\u00f6gl","year":"2018","journal-title":"Front Immunol"},{"key":"10.1016\/j.abd.2021.06.004_bib0165","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1007\/s004030050435","article-title":"Diversity of desmosomal proteins in regenerating epidermis: immunohistochemical study using a human skin organ culture model","volume":"291","author":"Moll","year":"1999","journal-title":"Arch Dermatol Res"},{"key":"10.1016\/j.abd.2021.06.004_bib0170","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1242\/jcs.03392","article-title":"Suprabasal Dsg2 expression in transgenic mouse skin confers a hyperproliferative and apoptosis-resistant phenotype to keratinocytes","volume":"120","author":"Brennan","year":"2007","journal-title":"J Cell Sci"},{"key":"10.1016\/j.abd.2021.06.004_bib0175","doi-asserted-by":"crossref","DOI":"10.1155\/2010\/410278","article-title":"Superficial Dsg2 Expression Limits Epidermal Blister Formation Mediated by Pemphigus Foliaceus Antibodies and Exfoliative Toxins","volume":"2010","author":"Brennan","year":"2010","journal-title":"Dermatol Res Pract"},{"key":"10.1016\/j.abd.2021.06.004_bib0180","doi-asserted-by":"crossref","first-page":"17043","DOI":"10.1074\/jbc.M113.489336","article-title":"Desmoglein 2 compensates for desmoglein 3 but does not control cell adhesion via regulation of p38 mitogen-activated protein kinase in keratinocytes","volume":"289","author":"Hartlieb","year":"2014","journal-title":"J Biol Chem"},{"key":"10.1016\/j.abd.2021.06.004_bib0185","doi-asserted-by":"crossref","first-page":"581370","DOI":"10.3389\/fimmu.2020.581370","article-title":"Dsg2 Upregulation as a Rescue Mechanism in Pemphigus","volume":"11","author":"Sigmund","year":"2020","journal-title":"Front Immunol"}],"container-title":["Anais Brasileiros de Dermatologia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0365059621003032?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0365059621003032?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T23:39:31Z","timestamp":1759793971000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0365059621003032"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3]]},"references-count":37,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["S0365059621003032"],"URL":"https:\/\/doi.org\/10.1016\/j.abd.2021.06.004","relation":{},"ISSN":["0365-0596"],"issn-type":[{"value":"0365-0596","type":"print"}],"subject":[],"published":{"date-parts":[[2022,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Autoantibodies against desmoglein 2 are not pathogenic in pemphigus","name":"articletitle","label":"Article Title"},{"value":"Anais Brasileiros de Dermatologia","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.abd.2021.06.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Sociedade Brasileira de Dermatologia. Published by Elsevier Espa\u00f1a, S.L.U.","name":"copyright","label":"Copyright"}]}}