{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T21:20:06Z","timestamp":1773091206323,"version":"3.50.1"},"reference-count":22,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"publisher","award":["21705053"],"award-info":[{"award-number":["21705053"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"publisher","award":["81871732"],"award-info":[{"award-number":["81871732"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["2017CFB117"],"award-info":[{"award-number":["2017CFB117"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Hubei Province health and family planning scientific research project","award":["J2017Q017"],"award-info":[{"award-number":["J2017Q017"]}]},{"name":"Wuhan Youth Science and Technology Plan","award":["2017050304010293"],"award-info":[{"award-number":["2017050304010293"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2019,10]]},"DOI":"10.1016\/j.aca.2019.05.015","type":"journal-article","created":{"date-parts":[[2019,5,16]],"date-time":"2019-05-16T17:26:40Z","timestamp":1558027600000},"page":"137-143","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Eliminating the secondary structure of targeting strands for enhancement of DNA probe based low-abundance point mutation detection"],"prefix":"10.1016","volume":"1075","author":[{"given":"Zhihao","family":"Ming","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianzhi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junbo","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0455-1080","authenticated-orcid":false,"given":"Xianjin","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.aca.2019.05.015_bib1","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.aca.2016.06.037","article-title":"Electrochemical DNA sensor-based strategy for sensitive detection of DNA demethylation and DNA demethylase activity","volume":"934","author":"Shen","year":"2016","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2019.05.015_bib2","doi-asserted-by":"crossref","first-page":"2311","DOI":"10.1021\/ac048703c","article-title":"Multiple and simultaneous detection of specific bacteria in enriched bacterial communities using a DNA microarray chip with randomly generated genomic DNA probes","volume":"77","author":"Byoung Chan","year":"2005","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2019.05.015_bib3","first-page":"136ra168","article-title":"Noninvasive identification and monitoring of cancer mutations by targeted deep sequencing of plasma DNA","volume":"4","author":"Tim","year":"2012","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.aca.2019.05.015_bib4","doi-asserted-by":"crossref","first-page":"4709","DOI":"10.1021\/cr900323b","article-title":"Binary probes for nucleic acid analysis","volume":"110","author":"Kolpashchikov","year":"2010","journal-title":"Chem. Rev."},{"key":"10.1016\/j.aca.2019.05.015_bib5","doi-asserted-by":"crossref","first-page":"4315","DOI":"10.1002\/anie.201411000","article-title":"Highly sensitive and robust linear probe for detection of mRNA in cells","volume":"54","author":"Asanuma","year":"2015","journal-title":"Angew Chem. Int. Ed. Engl."},{"key":"10.1016\/j.aca.2019.05.015_bib6","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1021\/ja053121d","article-title":"Reagentless, reusable, ultrasensitive electrochemical molecular beacon aptasensor","volume":"128","author":"Abd-Elgawad","year":"2006","journal-title":"J.\u00a0Am. Chem. Soc."},{"key":"10.1016\/j.aca.2019.05.015_bib7","doi-asserted-by":"crossref","first-page":"15311","DOI":"10.1021\/ja905068s","article-title":"An electrochemical sensor for single nucleotide polymorphism detection in serum based on a triple-stem DNA probe","volume":"131","author":"Yi","year":"2009","journal-title":"J.\u00a0Am. Chem. Soc."},{"key":"10.1016\/j.aca.2019.05.015_bib8","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1126\/science.1200520","article-title":"Scaling up digital circuit computation with DNA strand displacement cascades","volume":"332","author":"Lulu","year":"2011","journal-title":"Science"},{"key":"10.1016\/j.aca.2019.05.015_bib9","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.aca.2005.04.044","article-title":"A\u00a0straightforward separation scheme for concomitant Lu\u2013Hf and Sm\u2013Nd isotope ratio and isotope dilution analysis","volume":"543","author":"Le F\u00e8vre","year":"2005","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2019.05.015_bib10","doi-asserted-by":"crossref","first-page":"13090","DOI":"10.1039\/C5CC03761A","article-title":"Highly-efficient T4 DNA ligase-based SNP analysis using a ligation fragment containing a modified nucleobase at the end","volume":"51","author":"Jang","year":"2015","journal-title":"Chem. Commun."},{"key":"10.1016\/j.aca.2019.05.015_bib11","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.bios.2013.02.036","article-title":"Nanodiamond based sponges with entrapped enzyme: a novel electrochemical probe for hydrogen peroxide","volume":"46","author":"Gopalan","year":"2013","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.aca.2019.05.015_bib12","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1016\/j.cell.2013.01.031","article-title":"Beyond secondary structure: primary-sequence determinants license pri-miRNA hairpins for processing","volume":"152","author":"Auyeung","year":"2013","journal-title":"Cell"},{"key":"10.1016\/j.aca.2019.05.015_bib13","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1038\/nrg3296","article-title":"DNA secondary structures: stability and function of G-quadruplex structures","volume":"13","author":"Bochman","year":"2012","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.aca.2019.05.015_bib14","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1534\/genetics.115.176834","article-title":"The rate and molecular spectrum of spontaneous mutations in the GC-rich multichromosome genome of burkholderia cenocepacia","volume":"200","author":"Dillon","year":"2015","journal-title":"Genetics"},{"key":"10.1016\/j.aca.2019.05.015_bib15","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1007\/BF00163235","article-title":"Statistical analysis of vertebrate sequences reveals that long genes are scarce in GC-rich isochores","volume":"40","author":"Duret","year":"1995","journal-title":"J.\u00a0Mol. Evol."},{"key":"10.1016\/j.aca.2019.05.015_bib16","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0047924","article-title":"Genome-wide chromatin remodeling identified at GC-rich long nucleosome-free regions","volume":"7","author":"Schwarzbauer","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.aca.2019.05.015_bib17","doi-asserted-by":"crossref","first-page":"e29","DOI":"10.1093\/nar\/gkp1138","article-title":"New approach to real-time nucleic acids detection: folding polymerase chain reaction amplicons into a secondary structure to improve cleavage of Forster resonance energy transfer probes in 5'-nuclease assays","volume":"38","author":"Kutyavin","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.aca.2019.05.015_bib18","doi-asserted-by":"crossref","first-page":"5461","DOI":"10.1039\/c1cc11396h","article-title":"A\u00a0label-free fluorescent turn-on enzymatic amplification assay for DNA detection using ligand-responsive G-quadruplex formation","volume":"47","author":"Zhao","year":"2011","journal-title":"Chem. Commun."},{"key":"10.1016\/j.aca.2019.05.015_bib19","doi-asserted-by":"crossref","first-page":"7400","DOI":"10.1038\/srep07400","article-title":"A\u00a0G-quadruplex DNA-based, label-free and ultrasensitive strategy for microRNA detection","volume":"4","author":"Yan","year":"2014","journal-title":"Sci. Rep."},{"key":"10.1016\/j.aca.2019.05.015_bib20","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.aca.2011.02.058","article-title":"Fast, selective, and sensitive analysis of low-abundance peptides in human plasma by electromembrane extraction","volume":"716","author":"Balchen","year":"2012","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2019.05.015_bib21","doi-asserted-by":"crossref","first-page":"9422","DOI":"10.1039\/C7CC04816E","article-title":"Endonuclease IV based competitive DNA probe assay for differentiation of low-abundance point mutations by discriminating stable single-base mismatches","volume":"53","author":"Xu","year":"2017","journal-title":"Chem. Commun."},{"key":"10.1016\/j.aca.2019.05.015_bib22","doi-asserted-by":"crossref","first-page":"1964","DOI":"10.1039\/C1CC15360A","article-title":"Ultra-selective and sensitive DNA detection by a universal apurinic\/apyrimidinic probe-based endonuclease IV signal amplification system","volume":"48","author":"Xiao","year":"2012","journal-title":"Chem. Commun."}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267019305598?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267019305598?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T18:42:33Z","timestamp":1565116953000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267019305598"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10]]},"references-count":22,"alternative-id":["S0003267019305598"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2019.05.015","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2019,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Eliminating the secondary structure of targeting strands for enhancement of DNA probe based low-abundance point mutation detection","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2019.05.015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}