{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T23:56:53Z","timestamp":1788479813690,"version":"build-2803163510"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T00:00:00Z","timestamp":1598486400000},"content-version":"am","delay-in-days":300,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2019,11]]},"DOI":"10.1016\/j.aca.2019.07.035","type":"journal-article","created":{"date-parts":[[2019,7,22]],"date-time":"2019-07-22T17:25:57Z","timestamp":1563816357000},"page":"186-193","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":45,"special_numbering":"C","title":["Isolation and quantitation of exosomes isolated from human plasma via hydrophobic interaction chromatography using a polyester, capillary-channeled polymer fiber phase"],"prefix":"10.1016","volume":"1082","author":[{"given":"Lei","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terri F.","family":"Bruce","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sisi","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4276-5805","authenticated-orcid":false,"given":"R. Kenneth","family":"Marcus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.aca.2019.07.035_bib1","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1083\/jcb.201211138","article-title":"Extracellular vesicles: exosomes, microvesicles, and friends","volume":"200","author":"Raposo","year":"2013","journal-title":"J.\u00a0Cell Biol."},{"issue":"2","key":"10.1016\/j.aca.2019.07.035_bib2","doi-asserted-by":"crossref","first-page":"152","DOI":"10.3390\/genes4020152","article-title":"Signaling pathways in exosomes biogenesis, secretion and fate","volume":"4","author":"Urbanelli","year":"2013","journal-title":"Genes"},{"issue":"1\u20132","key":"10.1016\/j.aca.2019.07.035_bib3","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.jim.2011.06.024","article-title":"A\u00a0filtration-based protocol to isolate human plasma membrane-derived vesicles and exosomes from blood plasma","volume":"371","author":"Grant","year":"2011","journal-title":"J.\u00a0Immunol. Methods"},{"issue":"1","key":"10.1016\/j.aca.2019.07.035_bib4","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1479-5876-9-9","article-title":"Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages","volume":"9","author":"L\u00e4sser","year":"2011","journal-title":"J.\u00a0Transl. Med."},{"issue":"22","key":"10.1016\/j.aca.2019.07.035_bib5","doi-asserted-by":"crossref","first-page":"3354","DOI":"10.1002\/pmic.201300282","article-title":"Comparative proteomics evaluation of plasma exosome isolation techniques and assessment of the stability of exosomes in normal human blood plasma","volume":"13","author":"Kalra","year":"2013","journal-title":"Proteomics"},{"issue":"1","key":"10.1016\/j.aca.2019.07.035_bib6","doi-asserted-by":"crossref","DOI":"10.3390\/bioengineering6010008","article-title":"Characterization of plasma-derived extracellular vesicles isolated by different methods: a comparison study","volume":"6","author":"Serrano-Pertierra","year":"2019","journal-title":"Bioengin"},{"key":"10.1016\/j.aca.2019.07.035_bib7","doi-asserted-by":"crossref","first-page":"24316","DOI":"10.1038\/srep24316","article-title":"Low-density lipoprotein mimics blood plasma-derived exosomes and microvesicles during isolation and detection","volume":"6","author":"Sodar","year":"2016","journal-title":"Sci. Rep."},{"issue":"7","key":"10.1016\/j.aca.2019.07.035_bib8","doi-asserted-by":"crossref","first-page":"6641","DOI":"10.1021\/acsnano.7b00549","article-title":"Rapid isolation and detection of exosomes and associated biomarkers from plasma","volume":"11","author":"Ibsen","year":"2017","journal-title":"ACS Nano"},{"key":"10.1016\/j.aca.2019.07.035_bib9","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.jprot.2012.12.029","article-title":"Characterization and proteomic analysis of ovarian cancer-derived exosomes","volume":"80","author":"Liang","year":"2013","journal-title":"J.\u00a0Proteomics"},{"issue":"8","key":"10.1016\/j.aca.2019.07.035_bib10","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0103310","article-title":"Isolation and characterization of CD34+ blast-derived exosomes in acute myeloid leukemia","volume":"9","author":"Hong","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.aca.2019.07.035_bib11","doi-asserted-by":"crossref","first-page":"29289","DOI":"10.3402\/jev.v5.29289","article-title":"Isolation of biologically active and morphologically intact exosomes from plasma of patients with cancer","volume":"5","author":"Hong","year":"2016","journal-title":"J.\u00a0Extracell. Vesicles"},{"issue":"12","key":"10.1016\/j.aca.2019.07.035_bib12","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0145686","article-title":"Isolation of exosomes from blood plasma: qualitative and quantitative comparison of ultracentrifugation and size exclusion chromatography methods","volume":"10","author":"Baranyai","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.aca.2019.07.035_bib13","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.jim.2014.06.007","article-title":"Isolation of biologically-active exosomes from human plasma","volume":"411","author":"Muller","year":"2014","journal-title":"J.\u00a0Immunol. Methods"},{"issue":"5","key":"10.1016\/j.aca.2019.07.035_bib14","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1111\/bjh.12543","article-title":"Normal prion protein is expressed on exosomes isolated from human plasma","volume":"163","author":"Ritchie","year":"2013","journal-title":"Br. J. Haematol."},{"key":"10.1016\/j.aca.2019.07.035_bib15","first-page":"3","article-title":"Exosomes in plasma of patients with ovarian carcinoma: potential biomarkers of tumor progression and response to therapy","volume":"4","author":"Szajnik","year":"2013","journal-title":"Obstet. Gynecol. Suppl"},{"key":"10.1016\/j.aca.2019.07.035_bib16","doi-asserted-by":"crossref","first-page":"27031","DOI":"10.3402\/jev.v4.27031","article-title":"Optimized exosome isolation protocol for cell culture supernatant and human plasma","volume":"4","author":"Lobb","year":"2015","journal-title":"J.\u00a0Extracell. Vesicles"},{"issue":"10","key":"10.1016\/j.aca.2019.07.035_bib17","doi-asserted-by":"crossref","first-page":"3599","DOI":"10.1021\/acs.jproteome.8b00479","article-title":"Comparison of an optimized ultracentrifugation method versus size-exclusion chromatography for isolation of exosomes from human serum","volume":"17","author":"An","year":"2018","journal-title":"J.\u00a0Proteome Res."},{"issue":"7","key":"10.1016\/j.aca.2019.07.035_bib18","first-page":"3419","article-title":"Isolation of prostate cancer-related exosomes","volume":"34","author":"Mizutani","year":"2014","journal-title":"Anticancer Res."},{"issue":"1","key":"10.1016\/j.aca.2019.07.035_bib19","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1093\/annonc\/mdx542","article-title":"Surfaceome profiling enables isolation of cancer-specific exosomal cargo in liquid biopsies from pancreatic cancer patients","volume":"29","author":"Castillo","year":"2017","journal-title":"Ann. Oncol."},{"issue":"4","key":"10.1016\/j.aca.2019.07.035_bib20","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1002\/elps.201800417","article-title":"Exosome isolation and purification via hydrophobic interaction chromatography using a polyester, capillary-channeled polymer fiber phase","volume":"40","author":"Bruce","year":"2019","journal-title":"Electrophoresis"},{"issue":"1","key":"10.1016\/j.aca.2019.07.035_bib21","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0021-9673(02)01835-6","article-title":"Capillary-channeled polymer fibers as stationary phases in liquid chromatography separations","volume":"986","author":"Marcus","year":"2003","journal-title":"J.\u00a0Chromatogr. A"},{"issue":"1","key":"10.1016\/j.aca.2019.07.035_bib22","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/s00216-008-2457-2","article-title":"Nylon-6 capillary-channeled polymer (C-CP) fibers as a hydrophobic interaction chromatography stationary phase for the separation of proteins","volume":"393","author":"Stanelle","year":"2009","journal-title":"Anal. Bioanal. Chem."},{"issue":"5","key":"10.1016\/j.aca.2019.07.035_bib23","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1002\/jssc.201301205","article-title":"Initial evaluation of protein A modified capillary-channeled polymer fibers for the capture and recovery of immunoglobulin G","volume":"37","author":"Schadock-Hewitt","year":"2014","journal-title":"J.\u00a0Sep. Sci."},{"issue":"48","key":"10.1016\/j.aca.2019.07.035_bib24","doi-asserted-by":"crossref","first-page":"8410","DOI":"10.1039\/C6AY02737G","article-title":"Coupling of capillary-channeled polymer (C-CP) fibers for reversed phase liquid chromatography and ESI-MS for the determination of proteins in a urine matrix","volume":"8","author":"Wang","year":"2016","journal-title":"Anal. Methods."},{"key":"10.1016\/j.aca.2019.07.035_bib25","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.aca.2016.11.065","article-title":"Microwave-assisted grafting polymerization modification of nylon 6 capillary-channeled polymer fibers for enhanced weak cation exchange protein separations","volume":"954","author":"Jiang","year":"2017","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2019.07.035_bib26","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.chroma.2014.05.033","article-title":"Determination of pore size distributions in capillary-channeled polymer fiber stationary phases by inverse size-exclusion chromatography and implications for fast protein separations","volume":"1351","author":"Wang","year":"2014","journal-title":"J.\u00a0Chromatogr., A"},{"issue":"23","key":"10.1016\/j.aca.2019.07.035_bib27","doi-asserted-by":"crossref","first-page":"3270","DOI":"10.1002\/jssc.201200523","article-title":"Dynamic evaluation of polypropylene capillary-channeled fibers as a stationary phase in high-performance liquid chromatography","volume":"35","author":"Randunu","year":"2012","journal-title":"J.\u00a0Sep. Sci."},{"key":"10.1016\/j.aca.2019.07.035_bib28","doi-asserted-by":"crossref","DOI":"10.1007\/s00216-019-02022-7","article-title":"Isolation and quantification of human urinary exosomes by hydrophobic interaction chromatography on a polyester capillary-channeled polymer fiber stationary phase","author":"Huang","year":"2019","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.aca.2019.07.035_bib29","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.chroma.2018.11.068","article-title":"Evaluation of protein separations based on hydrophobic interaction chromatography using polyethylene terephthalate capillary-channeled polymer (C-CP) fiber phases","volume":"1585","author":"Wang","year":"2019","journal-title":"J.\u00a0Chromatogr., A"},{"issue":"4","key":"10.1016\/j.aca.2019.07.035_bib30","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1002\/elps.201800417","article-title":"Exosome isolation and purification via hydrophobic interaction chromatography using a polyester, capillary-channeled polymer fiber phase","volume":"40","author":"Bruce","year":"2019","journal-title":"Electrophoresis"},{"key":"10.1016\/j.aca.2019.07.035_bib31","series-title":"Unpublised Results","author":"Kelsey","year":"2019"},{"issue":"3","key":"10.1016\/j.aca.2019.07.035_bib32","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1007\/s00216-012-6163-8","article-title":"Microbore polypropylene capillary channeled polymer (C-CP) fiber columns for rapid reversed-phase HPLC of proteins","volume":"404","author":"Randunu","year":"2012","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.aca.2019.07.035_bib33","unstructured":"S. Huang, R.K. Marcus, unpublished results (2019)."},{"issue":"2","key":"10.1016\/j.aca.2019.07.035_bib34","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0168-1656(01)00237-1","article-title":"Hydrophobic interaction chromatography of proteins","volume":"87","author":"Queiroz","year":"2001","journal-title":"J.\u00a0Biotechnol."},{"issue":"2","key":"10.1016\/j.aca.2019.07.035_bib35","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.chroma.2004.11.002","article-title":"Hydrophobic interaction chromatography correctly refolding proteins assisted by glycerol and urea gradients","volume":"1061","author":"Li","year":"2004","journal-title":"J.\u00a0Chromatogr. A"},{"key":"10.1016\/j.aca.2019.07.035_bib36","series-title":"Exosome Capture and Recovery Characteristics via a Glycerol-Assisted Hydrophobic Interaction Chromatography Method on Polyester Capillary-Channeled Polymer (C-CP) Fiber Phases Manuscript in Preparation","author":"Huang","year":"2019"},{"issue":"3","key":"10.1016\/j.aca.2019.07.035_bib37","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1007\/s12013-013-9744-z","article-title":"Isolation of peripheral blood mononuclear cells using glycerol density gradient","volume":"68","author":"Aimola","year":"2014","journal-title":"Cell Biochem. Biophys."},{"issue":"6","key":"10.1016\/j.aca.2019.07.035_bib38","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1016\/j.nano.2011.04.003","article-title":"Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis","volume":"7","author":"Dragovic","year":"2011","journal-title":"Nanomedicine"},{"key":"10.1016\/j.aca.2019.07.035_bib39","doi-asserted-by":"crossref","first-page":"27031","DOI":"10.3402\/jev.v4.27031","article-title":"Optimized exosome isolation protocol for cell culture supernatant and human plasma","volume":"4","author":"Lobb","year":"2015","journal-title":"J.\u00a0Extracell. Vesicles"}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267019308438?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267019308438?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T19:13:38Z","timestamp":1757704418000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267019308438"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11]]},"references-count":39,"alternative-id":["S0003267019308438"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2019.07.035","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2019,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Isolation and quantitation of exosomes isolated from human plasma via hydrophobic interaction chromatography using a polyester, capillary-channeled polymer fiber phase","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2019.07.035","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}