{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:07:21Z","timestamp":1780963641991,"version":"3.54.1"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2020,8]]},"DOI":"10.1016\/j.aca.2020.05.046","type":"journal-article","created":{"date-parts":[[2020,5,23]],"date-time":"2020-05-23T08:28:38Z","timestamp":1590222518000},"page":"152-161","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":37,"special_numbering":"C","title":["Activatable fluorescent probe based on aggregation-induced emission for detecting hypoxia-related pathological conditions"],"prefix":"10.1016","volume":"1125","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2008-1664","authenticated-orcid":false,"given":"Lingfeng","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihe","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6739-0694","authenticated-orcid":false,"given":"Shuizhu","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.aca.2020.05.046_bib1","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/nrd4422","article-title":"Targeting hypoxia signalling for the treatment of ischaemic and inflammatory diseases","volume":"13","author":"Eltzschig","year":"2014","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.aca.2020.05.046_bib2","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1038\/nrc798","article-title":"Papillomaviruses and cancer: from basic studies to clinical application","volume":"2","author":"zur Hausen","year":"2002","journal-title":"Nat. Rev. Canc."},{"key":"10.1016\/j.aca.2020.05.046_bib3","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/j.cell.2014.02.055","article-title":"The intrinsic apoptosis pathway mediates the pro-longevity response to mitochondrial ROS in C.\u00a0elegans","volume":"157","author":"Yee","year":"2014","journal-title":"Cell"},{"key":"10.1016\/j.aca.2020.05.046_bib4","doi-asserted-by":"crossref","first-page":"7313","DOI":"10.7150\/thno.36755","article-title":"An activatable near-infrared chromophore for multispectral optoacoustic imaging of tumor hypoxia and for tumor inhibition","volume":"9","author":"Huang","year":"2019","journal-title":"Theranostics"},{"key":"10.1016\/j.aca.2020.05.046_bib5","doi-asserted-by":"crossref","first-page":"6423","DOI":"10.1158\/0008-5472.CAN-09-1285","article-title":"Epigenetic repression of DNA mismatch repair by inflammation and hypoxia in inflammatory bowel disease\u2013associated colorectal cancer","volume":"69","author":"Edwards","year":"2009","journal-title":"Canc. Res."},{"key":"10.1016\/j.aca.2020.05.046_bib6","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1038\/nrc3064","article-title":"Targeting hypoxia in cancer therapy","volume":"11","author":"Wilson","year":"2011","journal-title":"Nat. Rev. Canc."},{"key":"10.1016\/j.aca.2020.05.046_bib7","doi-asserted-by":"crossref","first-page":"10586","DOI":"10.1039\/C9SC03355F","article-title":"Hypoxia-activated NIR photosensitizer anchoring in the mitochondria for photodynamic therapy","volume":"10","author":"Xu","year":"2019","journal-title":"Chem. Sci."},{"key":"10.1016\/j.aca.2020.05.046_bib8","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1039\/C2SC21074F","article-title":"Enzymatic activation of nitro-aryl fluorogens in live bacterial cells for enzymatic turnover-activated localization microscopy","volume":"4","author":"Lee","year":"2013","journal-title":"Chem. Sci."},{"key":"10.1016\/j.aca.2020.05.046_bib9","doi-asserted-by":"crossref","first-page":"3983","DOI":"10.1038\/s41467-018-06499-1","article-title":"Activatable probes for diagnosing and positioning liver injury and metastatic tumors by multispectral optoacoustic tomography","volume":"9","author":"Wu","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.aca.2020.05.046_bib10","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.aca.2018.03.030","article-title":"Two-photon fluorescent probe for detection of nitroreductase and hypoxia-specific microenvironment of cancer stem cell","volume":"1024","author":"Liu","year":"2018","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2020.05.046_bib11","doi-asserted-by":"crossref","first-page":"2237","DOI":"10.1039\/C6CS00908E","article-title":"Fluorescent chemical probes for accurate tumor diagnosis and targeting therapy","volume":"46","author":"Gao","year":"2017","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.aca.2020.05.046_bib12","doi-asserted-by":"crossref","first-page":"1807960","DOI":"10.1002\/adfm.201807960","article-title":"A\u00a0nanoprobe for diagnosing and mapping lymphatic metastasis of tumor using 3D multispectral optoacoustic tomography owing to aggregation\/Deaggregation induced spectral change","volume":"29","author":"Wu","year":"2019","journal-title":"Adv. Funct. Mater."},{"issue":"1\u201312","key":"10.1016\/j.aca.2020.05.046_bib13","doi-asserted-by":"crossref","first-page":"5834","DOI":"10.1038\/ncomms6834","article-title":"Hypoxia-specific ultrasensitive detection of tumours and cancer cells in\u00a0vivo","volume":"6","author":"Zheng","year":"2015","journal-title":"Nat. Commun."},{"key":"10.1016\/j.aca.2020.05.046_bib14","doi-asserted-by":"crossref","first-page":"13028","DOI":"10.1002\/anie.201305784","article-title":"Development of azo-based fluorescent probes to detect different levels of hypoxia","volume":"52","author":"Piao","year":"2013","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.aca.2020.05.046_bib15","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1039\/C2CC37329G","article-title":"Carbon-dot-based ratiometric fluorescent sensor for detecting hydrogen sulfide in aqueous media and inside live cells","volume":"49","author":"Yu","year":"2013","journal-title":"Chem. Commun."},{"key":"10.1016\/j.aca.2020.05.046_bib16","doi-asserted-by":"crossref","first-page":"5928","DOI":"10.1039\/C5SC01113B","article-title":"H-Rubies, A new family of red emitting fluorescent pH sensors for living cells","volume":"6","author":"Despras","year":"2015","journal-title":"Chem. Sci."},{"key":"10.1016\/j.aca.2020.05.046_bib17","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1021\/acs.analchem.7b04407","article-title":"Real-time monitoring of endogenous cysteine levels in\u00a0vivo by near-infrared turn-on fluorescent probe with large Stokes shift","volume":"90","author":"Qi","year":"2018","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2020.05.046_bib18","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1021\/acsami.5b11023","article-title":"Pyrene derivative emitting red or near-infrared light with monomer\/excimer conversion and its application to ratiometric detection of hypochlorite","volume":"8","author":"Wu","year":"2016","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.aca.2020.05.046_bib19","doi-asserted-by":"crossref","first-page":"5361","DOI":"10.1039\/c1cs15113d","article-title":"Aggregation-induced emission","volume":"40","author":"Hong","year":"2011","journal-title":"Chem. Soc. Rev."},{"issue":"1\u201311","key":"10.1016\/j.aca.2020.05.046_bib20","doi-asserted-by":"crossref","first-page":"1904799","DOI":"10.1002\/adma.201904799","article-title":"Aggregation-induced nonlinear optical effects of AIEgen nanocrystals for ultradeep in\u00a0vivo bioimaging","author":"Zheng","year":"2019","journal-title":"Adv. Mater."},{"key":"10.1016\/j.aca.2020.05.046_bib21","doi-asserted-by":"crossref","first-page":"2471","DOI":"10.1039\/C5NR05051K","article-title":"Fluorescent nanoparticles based on AIE fluorogens for bioimaging","volume":"8","author":"Yan","year":"2016","journal-title":"Nanoscale"},{"key":"10.1016\/j.aca.2020.05.046_bib22","doi-asserted-by":"crossref","first-page":"4228","DOI":"10.1039\/C4CS00325J","article-title":"Biosensing by luminogens with aggregation-induced emission characteristics","volume":"44","author":"Kwok","year":"2015","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.aca.2020.05.046_bib23","doi-asserted-by":"crossref","first-page":"2112","DOI":"10.1021\/acssensors.8b00664","article-title":"An aggregation-induced emission-based \u201cturn-on\u201d fluorescent probe for facile detection of gaseous formaldehyde","volume":"3","author":"Zhao","year":"2018","journal-title":"ACS Sens."},{"key":"10.1016\/j.aca.2020.05.046_bib24","doi-asserted-by":"crossref","first-page":"2918","DOI":"10.1002\/asia.201600945","article-title":"A\u00a0new tetraphenylethylene-derived fluorescent probe for nitroreductase detection and hypoxic-tumor-cell imaging","volume":"11","author":"You","year":"2016","journal-title":"Chem. Asian J."},{"key":"10.1016\/j.aca.2020.05.046_bib25","doi-asserted-by":"crossref","DOI":"10.1002\/chem.201904675","article-title":"An unsymmetrical squaraine-based activatable probe for imaging lymphatic metastasis by responding to tumor hypoxia with MSOT and aggregation-enhanced fluorescent imaging","author":"Lin","year":"2019","journal-title":"Chem. Eur J."},{"key":"10.1016\/j.aca.2020.05.046_bib26","article-title":"Nanoaggregate probe for breast cancer metastasis through multispectral optoacoustic tomography and aggregation-induced NIR-I\/II fluorescence imaging","author":"Ouyang","year":"2019","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.aca.2020.05.046_bib27","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1039\/C3CS60271K","article-title":"Recent progress in the development of near-infrared fluorescent probes for bioimaging applications","volume":"43","author":"Guo","year":"2014","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.aca.2020.05.046_bib28","doi-asserted-by":"crossref","first-page":"2275","DOI":"10.1039\/C5SC04014K","article-title":"A\u00a0unique approach toward near-infrared fluorescent probes for bioimaging with remarkably enhanced contrast","volume":"7","author":"Gong","year":"2016","journal-title":"Chem. Sci."},{"key":"10.1016\/j.aca.2020.05.046_bib29","doi-asserted-by":"crossref","first-page":"1410","DOI":"10.1021\/acs.accounts.7b00087","article-title":"A\u00a0unique \u201cintegration\u201d strategy for the rational design of optically tunable near-infrared fluorophores","volume":"50","author":"Chen","year":"2017","journal-title":"Acc. Chem. Res."},{"key":"10.1016\/j.aca.2020.05.046_bib30","doi-asserted-by":"crossref","first-page":"12378","DOI":"10.1021\/jacs.7b06412","article-title":"Cell-permeant large Stokes shift dyes for transfection-free multicolor nanoscopy","volume":"139","author":"Butkevich","year":"2017","journal-title":"J.\u00a0Am. Chem. Soc."},{"key":"10.1016\/j.aca.2020.05.046_bib31","doi-asserted-by":"crossref","first-page":"26266","DOI":"10.1021\/acsami.5b08539","article-title":"Highly stable near-infrared fluorescent organic nanoparticles with a large Stokes shift for noninvasive long-term cellular imaging","volume":"7","author":"Zhang","year":"2015","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.aca.2020.05.046_bib32","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.aca.2018.05.032","article-title":"A\u00a0turn-on fluorescence probe based on aggregation-induced emission for leucine aminopeptidase in living cells and tumor tissue","volume":"1031","author":"Huang","year":"2018","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2020.05.046_bib33","doi-asserted-by":"crossref","first-page":"6188","DOI":"10.1039\/C7SC02189E","article-title":"Fluorometric probing of the lipase level as acute pancreatitis biomarkers based on interfacially controlled aggregation-induced emission (AIE)","volume":"8","author":"Shi","year":"2017","journal-title":"Chem. Sci."},{"key":"10.1016\/j.aca.2020.05.046_bib34","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.aca.2019.12.063","article-title":"A\u00a0mitochondria-targeting nitroreductase fluorescent probe with large Stokes shift and long-wavelength emission for imaging hypoxic status in tumor cells","volume":"1130","author":"Yang","year":"2020","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.aca.2020.05.046_bib35","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.bios.2014.07.024","article-title":"In\u00a0vivo imaging and detection of nitroreductase in zebrafish by a new near-infrared fluorescence off-on probe","volume":"63","author":"Li","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.aca.2020.05.046_bib36","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.aca.2015.04.006","article-title":"Determination of human serum semicarbazide-sensitive amine oxidase activity via flow injection analysis with fluorescence detection after online derivatization of the enzymatically produced benzaldehyde with 1,2-diaminoanthraquinone","volume":"881","author":"EI-Maghrabey","year":"2015","journal-title":"Anal. Chim. Acta"},{"issue":"1\u20139","key":"10.1016\/j.aca.2020.05.046_bib37","doi-asserted-by":"crossref","first-page":"107779","DOI":"10.1016\/j.dyepig.2019.107779","article-title":"A\u00a0resorufin-based fluorescent turn-on probe responsive to nitroreductase activity and its application to bacterial detection","volume":"171","author":"Yoon","year":"2019","journal-title":"Dyes Pigmt"},{"key":"10.1016\/j.aca.2020.05.046_bib38","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.talanta.2019.02.026","article-title":"An elegant nitroreductase responsive fluorescent probe for selective detection of pathogenic Listeria in\u00a0vitro and in\u00a0vivo","volume":"198","author":"Zhang","year":"2019","journal-title":"Talanta"},{"key":"10.1016\/j.aca.2020.05.046_bib39","doi-asserted-by":"crossref","first-page":"15974","DOI":"10.1021\/acs.analchem.9b04564","article-title":"Evolving a unique red-emitting fluorophore with an optically tunable hydroxy group for Imaging nitroreductase in cells, in tissues, and in\u00a0vivo","volume":"91","author":"Peng","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2020.05.046_bib40","article-title":"Nanoaggregate probe for breast cancer metastasis through multispectral optoacoustic tomography and aggregation-induced NIR-I\/II fluorescence imaging","author":"Ouyang","year":"2020","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.aca.2020.05.046_bib41","doi-asserted-by":"crossref","first-page":"15426","DOI":"10.1021\/acsami.9b04488","article-title":"Nitroreductase-activatable theranostic molecules with high PDT efficiency under mild hypoxia based on a TADF fluorescein derivative","volume":"17","author":"Liu","year":"2019","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.aca.2020.05.046_bib42","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1039\/C8TB02635A","article-title":"Development of a red-light emission hypoxia-sensitive two-photon fluorescent probe for in\u00a0vivo nitroreductase imaging","volume":"7","author":"Gebremedhin","year":"2019","journal-title":"J.\u00a0Mater. Chem. B"},{"key":"10.1016\/j.aca.2020.05.046_bib43","doi-asserted-by":"crossref","first-page":"8656","DOI":"10.1021\/jacs.5b02774","article-title":"A\u00a0\u201ccaged\u201d luciferin for imaging cell-cell contacts","volume":"137","author":"Porterfield","year":"2015","journal-title":"J.\u00a0Am. Chem. Soc."},{"key":"10.1016\/j.aca.2020.05.046_bib44","doi-asserted-by":"crossref","first-page":"13277","DOI":"10.1021\/ja505795s","article-title":"Aminoluciferins extend firefly luciferase bioluminescence into the near-infrared and can be preferred substrates over d-luciferin","volume":"136","author":"Mofford","year":"2014","journal-title":"J.\u00a0Am. Chem. Soc."},{"key":"10.1016\/j.aca.2020.05.046_bib45","doi-asserted-by":"crossref","first-page":"5610","DOI":"10.1021\/acs.analchem.6b01160","article-title":"Real-time bioluminescence imaging of nitroreductase in mouse model","volume":"88","author":"Feng","year":"2016","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2020.05.046_bib46","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1038\/nature04542","article-title":"Structural basis for the spectral difference in luciferase bioluminescence","volume":"440","author":"Nakatsu","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.aca.2020.05.046_bib47","doi-asserted-by":"crossref","first-page":"6585","DOI":"10.1021\/acs.analchem.9b00298","article-title":"Targeted myocardial hypoxia imaging using a nitroreductase-activatable near-infrared fluorescent nanoprobe","volume":"91","author":"Fan","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2020.05.046_bib48","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/S1471-4914(01)02090-1","article-title":"HIF-1 and tumor progression: pathophysiology and therapeutics","volume":"7","author":"Semenza","year":"2001","journal-title":"Trends Mol. Med."},{"key":"10.1016\/j.aca.2020.05.046_bib49","doi-asserted-by":"crossref","first-page":"6025","DOI":"10.7150\/thno.26607","article-title":"Hypoxia-triggered single molecule probe for high-contrast NIR II\/PA tumor imaging and robust photothermal therapy","volume":"8","author":"Meng","year":"2018","journal-title":"Theranostics"},{"key":"10.1016\/j.aca.2020.05.046_bib50","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.bios.2018.08.014","article-title":"Near-infrared off-on fluorescence probe activated by NTR for in\u00a0vivo hypoxia imaging","volume":"119","author":"Zheng","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.aca.2020.05.046_bib51","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1111\/cns.12016","article-title":"AKT\/GSK3\u03b2-dependent autophagy contributes to the neuroprotection of limb remote ischemic postconditioning in the transient cerebral ischemic rat model","volume":"18","author":"Qi","year":"2012","journal-title":"CNS Neurosci. Ther."},{"key":"10.1016\/j.aca.2020.05.046_bib52","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ejpb.2015.09.020","article-title":"Treatment of stroke with liposomal neuroprotective agents under cerebral ischemia conditions","volume":"97","author":"Fukuta","year":"2015","journal-title":"Eur. J. Pharm. Biopharm."}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267020305985?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267020305985?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T19:22:57Z","timestamp":1757704977000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267020305985"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8]]},"references-count":52,"alternative-id":["S0003267020305985"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2020.05.046","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2020,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Activatable fluorescent probe based on aggregation-induced emission for detecting hypoxia-related pathological conditions","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2020.05.046","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}