{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T17:58:14Z","timestamp":1792000694164,"version":"4.4.0"},"reference-count":27,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81972017"],"award-info":[{"award-number":["81972017"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008750","name":"Shanghai Hospital Development Center","doi-asserted-by":"publisher","award":["SHDC2020CR3006A"],"award-info":[{"award-number":["SHDC2020CR3006A"]}],"id":[{"id":"10.13039\/501100008750","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Analytica Chimica Acta"],"published-print":{"date-parts":[[2023,2]]},"DOI":"10.1016\/j.aca.2023.340812","type":"journal-article","created":{"date-parts":[[2023,1,7]],"date-time":"2023-01-07T02:37:44Z","timestamp":1673059064000},"page":"340812","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"special_numbering":"C","title":["An integrated dual-layer microfluidic platform for multiple respiratory viruses screening"],"prefix":"10.1016","volume":"1242","author":[{"given":"Hua","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingsong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9887-8879","authenticated-orcid":false,"given":"Shun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.aca.2023.340812_bib1","doi-asserted-by":"crossref","first-page":"9959","DOI":"10.18632\/aging.103344","article-title":"Why does COVID-19 disproportionately affect older people?","volume":"12","author":"Mueller","year":"2020","journal-title":"Aging"},{"key":"10.1016\/j.aca.2023.340812_bib2","doi-asserted-by":"crossref","first-page":"e238","DOI":"10.1016\/S1473-3099(20)30484-9","article-title":"Comparing SARS-CoV-2 with SARS-CoV and influenza pandemics","volume":"20","author":"Petersen","year":"2020","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.aca.2023.340812_bib3","doi-asserted-by":"crossref","first-page":"473","DOI":"10.3389\/fpubh.2020.00473","article-title":"Modeling the onset of symptoms of COVID-19","volume":"8","author":"Larsen","year":"2020","journal-title":"Front. Public Health"},{"key":"10.1016\/j.aca.2023.340812_bib4","doi-asserted-by":"crossref","first-page":"A6","DOI":"10.1016\/j.vaccine.2020.09.048","article-title":"The disease burden of influenza beyond respiratory illness","volume":"39","author":"Macias","year":"2021","journal-title":"Vaccine"},{"key":"10.1016\/j.aca.2023.340812_bib5","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1097\/QCO.0000000000000504","article-title":"Invasive pulmonary aspergillosis complicating severe influenza: epidemiology, diagnosis and treatment","volume":"31","author":"Vanderbeke","year":"2018","journal-title":"Curr. Opin. Infect. Dis."},{"key":"10.1016\/j.aca.2023.340812_bib6","doi-asserted-by":"crossref","first-page":"e262","DOI":"10.7861\/clinmed.2020-0598","article-title":"Lessons of the month: Co-infection with SARS-CoV-2 and influenza B virus in a patient with community-acquired pneumonia","volume":"20","author":"Coutinho","year":"2020","journal-title":"Clin. Med."},{"key":"10.1016\/j.aca.2023.340812_bib7","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.jmii.2020.05.013","article-title":"Co-infections among patients with COVID-19: the need for combination therapy with non-anti-SARS-CoV-2 agents?","volume":"53","author":"Lai","year":"2020","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"10.1016\/j.aca.2023.340812_bib8","doi-asserted-by":"crossref","first-page":"239","DOI":"10.34172\/aim.2020.04","article-title":"Co-Infection of coronavirus disease 2019 and influenza A: a report from Iran","volume":"23","author":"Khodamoradi","year":"2020","journal-title":"Arch. Iran. Med."},{"key":"10.1016\/j.aca.2023.340812_bib9","doi-asserted-by":"crossref","first-page":"1324","DOI":"10.3201\/eid2606.200299","article-title":"Co-Infection with SARS-CoV-2 and influenza A virus in patient with pneumonia, China","volume":"26","author":"Wu","year":"2020","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.aca.2023.340812_bib10","article-title":"Co-Infection with influenza A and COVID-19","volume":"7","author":"Konala","year":"2020","journal-title":"Eur.J. Case Rep. Intern.Med."},{"key":"10.1016\/j.aca.2023.340812_bib11","doi-asserted-by":"crossref","DOI":"10.1002\/sstr.202100034","article-title":"A colorimetric test to differentiate patients infected with influenza from COVID-19","author":"Kozlowski","year":"2021","journal-title":"Small Struct."},{"key":"10.1016\/j.aca.2023.340812_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.jviromet.2020.113974","article-title":"Value of swab types and collection time on SARS-COV-2 detection using RT-PCR assay","volume":"286","author":"Liu","year":"2020","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.aca.2023.340812_bib13","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2020.25.50.2000568","article-title":"Estimating the false-negative test probability of SARS-CoV-2 by RT-PCR","volume":"25","author":"Wikramaratna","year":"2020","journal-title":"Euro Surveill."},{"key":"10.1016\/j.aca.2023.340812_bib14","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.jviromet.2016.10.010","article-title":"Rapid and sensitive detection of Zika virus by reverse transcription loop-mediated isothermal amplification","volume":"238","author":"Wang","year":"2016","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.aca.2023.340812_bib15","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.jviromet.2018.02.012","article-title":"Multiplexed RT-LAMP assay for detection of group M HIV-1 in plasma or whole blood","volume":"255","author":"Curtis","year":"2018","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.aca.2023.340812_bib16","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkaa099","article-title":"Real-time kinetics and high-resolution melt curves in single-molecule digital LAMP to differentiate and study specific and non-specific amplification","volume":"48","author":"Rolando","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.aca.2023.340812_bib17","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1126\/science.aar6245","article-title":"CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity","volume":"360","author":"Chen","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.aca.2023.340812_bib18","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1126\/science.aaq0179","article-title":"Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6","volume":"360","author":"Gootenberg","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.aca.2023.340812_bib19","doi-asserted-by":"crossref","first-page":"2339","DOI":"10.1021\/acssynbio.0c00057","article-title":"Application of CRISPR-cas12a enhanced fluorescence assay coupled with nucleic acid amplification for the sensitive detection of african swine fever virus","volume":"9","author":"Tao","year":"2020","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.aca.2023.340812_bib20","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1038\/s41587-020-0513-4","article-title":"CRISPR-Cas12-based detection of SARS-CoV-2","volume":"38","author":"Broughton","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.aca.2023.340812_bib21","doi-asserted-by":"crossref","first-page":"4906","DOI":"10.1038\/s41467-020-18615-1","article-title":"Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection","volume":"11","author":"Nguyen","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.aca.2023.340812_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.virusres.2020.198129","article-title":"An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2","volume":"288","author":"Ali","year":"2020","journal-title":"Virus Res."},{"key":"10.1016\/j.aca.2023.340812_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2020.112642","article-title":"Contamination-free visual detection of SARS-CoV-2 with CRISPR\/Cas12a: a promising method in the point-of-care detection","author":"Chen","year":"2020","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.aca.2023.340812_bib24","doi-asserted-by":"crossref","first-page":"16204","DOI":"10.1021\/acs.analchem.0c04047","article-title":"Isothermal amplification and ambient visualization in a single tube for the detection of SARS-CoV-2 using loop-mediated amplification and CRISPR Technology","volume":"92","author":"Pang","year":"2020","journal-title":"Anal. Chem."},{"key":"10.1016\/j.aca.2023.340812_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2020.112766","article-title":"opvCRISPR: one-pot visual RT-LAMP-CRISPR platform for SARS-cov-2 detection","volume":"172","author":"Wang","year":"2021","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.aca.2023.340812_bib26","doi-asserted-by":"crossref","first-page":"4711","DOI":"10.1038\/s41467-020-18575-6","article-title":"Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay","volume":"11","author":"Ding","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.aca.2023.340812_bib27","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1002\/elps.201300511","article-title":"Fast and versatile fabrication of PMMA microchip electrophoretic devices by laser engraving","volume":"35","author":"Moreira Gabriel","year":"2014","journal-title":"Electrophoresis"}],"container-title":["Analytica Chimica Acta"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267023000338?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0003267023000338?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T11:41:32Z","timestamp":1762083692000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0003267023000338"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2]]},"references-count":27,"alternative-id":["S0003267023000338"],"URL":"https:\/\/doi.org\/10.1016\/j.aca.2023.340812","relation":{},"ISSN":["0003-2670"],"issn-type":[{"value":"0003-2670","type":"print"}],"subject":[],"published":{"date-parts":[[2023,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An integrated dual-layer microfluidic platform for multiple respiratory viruses screening","name":"articletitle","label":"Article Title"},{"value":"Analytica Chimica Acta","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.aca.2023.340812","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"340812"}}